• 대한한의학회지
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• 제25권2호
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• pp.67-76
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• 2004

Objectives : This study was designed to investigate the protective mechanisms of Palmul-tang on hypoxia-induced cytotoxicity in H9c2 cardiomyoblast cells. Methods : In this study, we used H9c2 cells. Cells were subjected to hypoxia in the absence and presence of $1000\mu\textrm{g}/ml$ Palmul-tang for 24 hrs. Cells were treated with various concentrations of Palmul-tang for 24 hrs. Cell viability was measured by MTT assay. Hypoxia markedly decreased the viability of H9c2 cells, which was characterized with apparent apoptotic features such as chromatin condensation as well as fragmentation of genomic DNA and nuclei. Results : Palmul-tang significantly reduced hypoxia-induced cell death and apoptotic characteristics. Also, Palmul-tang prevented mitochondrial dysfunction including the disruption of mitochondrial membrane permeability transition (MPT) and an increase in expression of apoptogenic Bcl-2 proteins in hypoxia-H9c2 cells. Conclusions; This study suggests that the protective effects of Palmul-tang against hypoxic damages may be mediated by the modulation of Bcl-2, Bax expression.

• 펄프종이기술
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• 제31권2호
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• pp.70-76
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• 1999

This study was to measure the potential of nonwoody fibrous material, kenaf. Whole stalk of kenaf, Hibiscus cannabinus was separated by two parts of bast and core portion, and cooked separately by alkaline method. Morphological characteristic was evaluated using confocal laser scanning microscope (CLSM) and fiber quality analyzer(FQA). The strength properties of handsheets, made by different mixing ration between kenaf base and core fibers, were measured. Cross-sectional area of bast fibers was smaller than that of core fibers, but the bast fibers had a thick cell wall and narrow lumen area. Bast fibers were longer in length than core fibers. Core fibers had thin cell walls, broad lumen areas, and short lengths, and they had collapsed shape even in water. These characteristics of core fibers affected strength properties of handsheet positively. When the amount of core fibers increased, the strength properties of handsheet were increased. When the amount of bast fibers increased, the handsheet had rougher surface and higher air permeability.

• Journal of Microbiology and Biotechnology
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• 제17권9호
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• pp.1573-1578
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• 2007

An increasing number of applications is being developed for the use of nanoparticles in various fields. We investigated possible toxicities of nanoparticles in cell culture and in mice. Nanoparticles tested were Zn (300 nm), Fe (100 nm), and Si (10-20, 40-50, and 90-110 nm). The cell lines used were brain, liver, stomach, and lung from humans. In the presence of nanopaticles, mitochodrial activity decreased zero to 15%. DNA contents decreased zero to 20%, and glutathione production increased zero to 15%. None of them showed a dose dependency. Plasma membrane permeability was not altered by nanoparticles. In the case of Si, different sizes of the nanoparticles did not affect cytotoxicity. The cytotoxicity was also shown to be similar in the presence of micro-sized ($45\;{\mu}m$) Si particles. Organs from mice fed with nanoparticles showed nonspecific hemorrhage, lymphocytic infiltration, and medullary congestion. A treatment with the micro-sized particle showed similar results, suggesting that the acute in vivo toxicity was not altered by nano-sized particles.

• 한국막학회:학술대회논문집
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• 한국막학회 2003년도 The 4th Korea-Italy Workshop
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• pp.29-32
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• 2003

Polymer electrolyte membranes are developed for the applications to facilitated transport membranes, fuel cells and solar cells. The polymer electrolyte membranes containing silver salt show the remarkably high separation performance for olefin/paraffin mixture in the solid state; the propylene permeance is 45 GPU and the ideal selectivity of propylene/propane is 15,000. For fuel cell membranes, the effects of the presence and size of the proton transport channels on the proton conductivity and methanol permeability were investigated. The cell performance for dye-sensitized solar cells employing polymer electrolytes are measured under light illumination. The overall energy conversion efficiency reaches 5.44 % at 10 ㎽/$\textrm{cm}^2$, to our knowledge the highest value ever reported in the polymer electrolytes.

• 한국지반환경공학회 논문집
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• 제13권1호
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• pp.37-43
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• 2012

일반적으로 수면과 접해 있는 호안이나 제방의 법면은 강우 침투로 인해 지반이 포화되어 침식에 약하고 유수로 인한 붕괴가 발생하기 쉽다. 따라서 사면 및 호안 제방을 축조할 경우, 비탈면 침식 및 세굴로 인해 붕괴가 발생하지 않도록 법면보호용 보강재를 설치해야 한다. 본 논문에서는 이러한 법면보호 보강재 중에서 기존의 3차원 지반보강재인 지오셀의 단점을 보완하고, 보다 친환경적인 보강재로 개발된 지오콤의 원지반과의 마찰특성, 수평투수능력, 식생의 뿌리활착 능력을 파악하기 위해 실내시험을 수행하였다. 중형전단시험 결과, 지오콤 보강지반의 내부마찰력은 기존 보강지반의 강도정수보다 증가하는 것으로 나타났고, 수평투수시험에서도 기존의 보강재보다 수평투수계수가 큰 것으로 나타났다. 이러한 결과는 셀 끝단의 구조가 기존 보강재와 달리 날카로운 돌기를 형성하고 있고, 셀의 내부표면이 망상 구조를 형성하고 있기 때문으로 판단된다.

• 한국구조물진단유지관리공학회 논문집
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• 제8권2호
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• pp.123-136
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• 2004

Terzaghi의 1차원 압밀이론은 그 유도과정에 있어 몇 가지 중요한 가정사항을 내포하고 있으며, 이로인해 이 이론을 연약 점토지반의 압밀거동에 적용하는데는 많은 모순이 발생할 수 밖에 없다. 특히, 미소변형 및 선형 물질함수에 대한 가정은 실제 현장의 압밀현상과 비교할 때 많은 오류를 발생시키는 원인으로 작용한다. 이러한 이유에서, Gibson 등은 물질함수의 비선형성을 고려할 수 있는 1차원 비선형 유한변형률 압밀이론에 대한 엄밀해를 발표하였다. 그러나, 이 이론은 연직배수공법이 적용된 일반적인 연약 점토지반의 압밀현상에는 적용시킬 수 없다는 단점을 내포하고 있다. 본 연구에서는 포화된 지반의 수직 및 수평방향 배수를 고려하며 지반의 자중 및 투수성과 압축성에 대한 물질함수의 비선형적 특성을 반영할 수 있는 압밀모델을 제안하였다. 또한 제안된 모델을 실제 압밀현상에 적용하기 위한 수치해석 기법을 개발하였다. 수치해석에 이용된 물질함수의 특성은 표준압밀 시험 및 로우셀 시험, 개량 표준압밀 시험등을 이용하여 산정되었다.

• Journal of Ginseng Research
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• 제37권1호
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• pp.108-116
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• 2013

For the improvement of ginsenoside bioavailability, the ginsenosides of fermented red ginseng by Phellinus linteus (FRG) were examined with respect to bioavailability and physiological activity. The polyphenol content of FRG ($19.14{\pm}0.50$ mg/g) was significantly higher (p<0.05) compared with that of non-fermented red ginseng (NFRG, $11.31{\pm}1.15$ mg/g). The antioxidant activities in FRG, such as 2,2'-diphenyl-1-picrylhydrazyl, 2,2-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid, and ferric reducing antioxidant power, were significantly higher (p<0.05) than those in NFRG. The HPLC analysis results showed that the FRG had a high level of ginsenoside metabolites. The total ginsenoside contents in NFRG and FRG were $41.65{\pm}1.53$ mg/g and $50.12{\pm}1.43$ mg/g, respectively. However, FRG had a significantly higher content ($33.90{\pm}0.97$ mg/g) of ginsenoside metabolites (Rg3, Rg5, Rk1, compound K, Rh1, F2, and Rg2) compared with NFRG ($14.75{\pm}0.46$ mg/g). The skin permeability of FRG was higher than that of NFRG using Franz diffusion cell models. In particular, after 3 h, the skin permeability of FRG was significantly higher (p<0.05) than that of NFRG. Using a rat everted intestinal sac model, FRG showed a high transport level compared with NFRG after 1 h. FRG had dramatically improved bioavailability compared with NFRG as indicated by skin permeation and intestinal permeability. The significantly greater bioavailability of FRG may have been due to the transformation of its ginsenosides by fermentation to more easily absorbable forms (ginsenoside metabolites).

• 멤브레인
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• 제15권2호
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• pp.165-174
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• 2005

본 연구에서는 SEBS와 여러 가지의 유기화물로 처리된 MMT type의 clay를 이용하여 SSEBS-clay 하이브리드 막을 용액법으로 제조하였다. clay의 함량은 5 phr로 고정하였다. 용액법을 사용하여 clay를 SEBS에 분산시켰으며, 제조된 SSEBS-clay 하이브리드에서 clay의 특성피크가 완전히 박리되거나 이동하는 XRD 결과로부터 고분자의 clay 층간삽입을 확인하였다. Clay의 종류에 따라서 제조된 SSEBS-clay 하이브리드 막의 가스투과도, 기계적 물성 및 열적 성질을 측정하였다. SSEBS-clay 하이브리드 막은 clay 자체의 도입과 층간거리의 확대로 기체분자의 tortuosity를 증가시켜서 가스투과도를 저하시키는 것을 확인하였다.

• Preventive Nutrition and Food Science
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• 제14권3호
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• pp.201-207
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• 2009

In an effort to improve ginsenoside bioavailability, the ginsenosides of fermented red ginseng were examined with respect to bioavailability and physiological activity. The results showed that the fermented red ginseng (FRG) had a high level of ginsenoside metabolites. The total ginsenoside contents in non-fermented red ginseng (NFRG) and FRG were 35715.2 ${\mu}g$/mL and 34822.9 ${\mu}g$/mL, respectively. However, RFG had a higher content (14914.3 ${\mu}g$/mL) of ginsenoside metabolites (Rg3, Rg5, Rk1, CK, Rh1, F2, and Rg2) compared to NFRG (5697.9 ${\mu}g$/mL). The skin permeability of RFG was higher than that of NFRG using Franz diffusion cells. Particularly, after 5 hr, the skin permeability of RFG was significantly (p<0.05) higher than that of NFRG. Using everted instestinal sacs of rats, RFG showed a high transport level (10.3 mg of polyphenols/g sac) compared to NFRG (6.67 of mg of polyphenols/g sac) after 1 hr. After oral administration of NFRG and FRG to rats, serum concentrations were determined by HPLC. Peak concentrations of Rk1, Rh1, Rc, and Rg5 were approximately 1.64, 2.35, 1.13, and 1.25-fold higher, respectively, for FRG than for NFRG. Furthermore, Rk1, Rh1, and Rg5 increased more rapidly in the blood by the oral administration of FRG versus NFRG. FRG had dramatically improved bioavailability compared to NFRG as indicated by skin permeation, intestinal permeability, and ginsenoside levels in the blood. The significantly greater bioavailability of FRG may have been due to the transformation of its ginsenosides by fermentation to more easily absorbable forms (ginsenoside metabolites).

• 한국환경보건학회지
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• 제39권4호
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• pp.360-368
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• 2013

Objectives: Phthalates are used in a large variety of products including as coatings of pharmaceutical tablets, film formers, stabilizers, dispersants, emulsifying agents, and suspending agents. They have been the subject of great public concern in recent years. The extensive uses of this material have attracted attention and issues regarding its safety have been raised. Methods: In this study, three types of phthalate skin permeation were studied using matrixes such as ointments, creams and lotions in vitro. The absorption of phthalate diesters [Dimethyl phthalate (DMP), Di-n-propyl phthalate (DPP) and Di-n-pentyl phthalate (DNPP)] using film former has been measured in vitro through rat skin. Epidermal membranes were set up in Franz diffusion cells and their permeability to PBS measured in order to establish the integrity of the skin before the phthalates were applied to the epidermal surface. Results: Absorption rates for each phthalate ester were determined and permeability assessment made to quantify any irreversible alterations in barrier function due to contact with the esters. Types of phthalate in vitro experimental results quickly appeared in the following order DMP > DPP ${\geq}$ DNPP. Conclusions: In the experimental results, lotion> cream> ointment, and the permeation rate of lotion with a great amount of moisture was the fastest. Skin permeation rate is generally influenced by the chemical characteristics of a given chemical, such as molecular weight and lipophilicity. As the esters became more lipophilic and less hydrophilic, the rate of absorption decreased.