• Journal of the Korean Applied Science and Technology
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• v.25 no.3
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• pp.370-379
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• 2008

Akt, a serine/threonine protein kinase as a viral oncogene, is a critical regulator of PI3K-mediated cell proliferation and survival. On translocation, Akt is phosphorylated and activated, ultimately resulting in stimulation of cell growth and survival. As a part of our program toward the novel Akt1 inhibitors with potent activity over PI3K signaling pathway, we found primary hit compound 2 with an $IC_{50}$ value of $620\mu}M$ from protein kinase focused library. Based on the structural features of 2, new 1,3,4-thiadiazole derivatives were designed by the introduction of aromatic and heteroaromatic moieties onto thiadiazole nucleus. In this work, a series of 1,3,4-thiadiazole derivatives 1a-1 were synthesized and evaluated for Akt1 inhibitory activity.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.12 no.8
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• pp.3911-3925
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• 2018

This paper proposes a novel steganography method to hide secret data during the generation of tangle patterns. Different from the traditional steganography based on modifying natural images, we propose to construct stego images according to the secret messages. We first create a model to group a selected image contour, and define some basic operations to generate various pattern cells. During data hiding, we create a cell library to establish the relationships between cells and secret data. By painting the cell inside the image contour, we create a dense tangle pattern to carry secret data. With the proposed method, a recipient can extract the secret data correctly. Experimental results show that the proposed method has a flexible embedding capacity. The constructed stego tangle image has good visual effects, and is secure against adversaries. Meanwhile, the stego tangle pattern is also robust to JPEG compression.

• Animal cells and systems
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• v.7 no.2
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• pp.159-164
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• 2003

The RAD3 gene of Saccharomyces cerevisiae is required for excision repair and is essential for cell viability. The RAD3 encoded protein possesses a single stranded DNA-dependent ATPase and DNA and DNA-RNA helicase activities. To examine the extent of conservation of structure and function of a S. pombe RAD3 during eukaryotic evolution, the RAD3 homolog gene was isolated by screening of genomic DNA library. The isolated gene was designated as HRD3 (homolog of RAD3 gene). Southern blot analysis confirmed that S. pombe chromosome contains the same DNA as HRD3 gene and this gene exists as a single copy in S. pombe. The transcript of 2.8 kb was detected by Northern blot analysis, The level of transcripts increased by ultraviolet (UV) irradiation, indicating that HRD3 is one of the UV-inducible genes in S. pombe. Furthermore, the predicted partial sequence of HRD3 protein has 60％ identity to S. cerevisiae RAD3 gene. This homology was particularly striking in the regions identified as being conserved in a group of DNA helicases. Gene deletion experiments indicate that the HRD3 gene is essential for viability and DNA repair function. These observations suggest evolutionary conservation of other protein components with which HRD3 might interact in mediating its DNA repair and viability functions.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.27 no.6C
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• pp.594-600
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• 2002

A data manipulation unit capable of bit partitioned shift and various multimedia data type conversions in addition to conventional shift, is presented. Utilizing the similarity between the data type conversion and the shift, the addition of small amount of interconnections to conventional barrel shifter enables data type conversion as well as shift operations with minimal hardware overhead. The presented data manipulation unit is composed of the shifter block for conventional shift and a pack and a unpack block. It has been designed with verilog HDL and the VLSI implementation results using compass 0.6 um standard cell are discussed.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.38C no.9
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• pp.781-786
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• 2013

Multiplexing information over parallel data channels based on RF MIMO concept is possible to achieve considerable data rates over large transmission ranges with just a single transmitting element. Visual multiplexing MIMO techniques will send independent streams of bits using the multiple elements of the light transmitter array and recording over a group of camera pixels can further enhance the data rates. The proposed system is a combination of the reliance on computer vision algorithms for tracking and OOK cell frame modulation. LED array are controlled to transmit message in the form of digital information using ON-OFF signaling with ON-OFF pulses (ON = bit 1, OFF = bit 0). A camera captures image frames of the array which are then individually processed and sequentially decoded to retrieve data. To demodulated data transmission, a motion tracking algorithm is implemented in OpenCV (Open source Computer Vision library) to classify the transmission pattern. One of the most advantages of proposed architecture is Computer Vision (CV) based image analysis techniques which can be used to spatially separate signals and remove interferences from ambient light. It will be the future challenges and opportunities for mobile communication networking research.

• The Journal of Information Technology
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• v.3 no.3
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• pp.57-65
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• 2000

We have proposed BIST method and circuit for embedded 16M SDRAM with logic. It can test the AC parameter of embedded 16M SDRAM using the BIST circuit capable of detecting the address of a fail cell of a 16M SDRAM installed in an Merged Memory with Logic(MML) generating the information of repair for redundancy circuit. The function and AC parameter of the embedded memory can also be tested using the proposed BIST method. The total gate of the BIST circuit is approximately 4,500 in the case of synthesizing by $0.25\mu\textrm{m}$ cell library. and verify the result of Verilog simulation. The test time of each one AC parameter is about 200ms using 2Y-March 14N algorithm.

• Nuclear Engineering and Technology
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• v.49 no.6
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• pp.1287-1300
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• 2017

Based on the observation that ignoring the angle dependency of multigroup resonance cross sections within a fuel pellet would result in nontrivial underestimation of the spatial self-shielding of flux, a parametrized spectral superhomogenization (SPH) factor library (PSSL) method is developed as a practical means of resolving the problem. Region-wise spectral SPH factors are calculated by the normal and transport corrected SPH iterations after ultrafine group slowing down calculations over various light water reactor pin-cell configurations. The parametrization is done with fuel temperature, U-238 number density, fuel radius, moderator source represented by ${\Sigma}_{mod}V_{mod}$, and the number density ratio of resonance nuclides to that of U-238 in a form of resonance interference correction factors. The parametrization is successful in that the root mean square errors of the interpolated SPH factors over the fuel regions of various pin-cells are within 0.1%. The improvement in reactivity error of the PSSL method is shown to be superior to that by the original SPH method in that the reactivity bias of -200 pcm to -300 pcm vanishes almost completely. It is demonstrated that the environment effect takes only about 4% in the reactivity improvement so that the pin-cell based PSSL method is effective in the assembly problems.

• Natural Product Sciences
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• v.8 no.4
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• pp.133-136
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• 2002

GC-MS data on the volatile oil (CS-oil) of Chrysanthemum sibiricum herbs led to the identification of 2-methoxythioanisol, (+)-camphor, geraniol, citral, thymol, eugenol, ${\beta}-caryophyllene$ oxide, ${\beta}-caryophyllene$, ${\beta}-eudesmol$, juniper camphor together with an unknown substance using the mass spectral library and literature data. CS-oil exhibited significant cytotoxicities on HL-60 $(IC_{50}\;12.5\;{\mu}g/ml)$ cell and mild on HepG-2 cell $(IC_{50}\;102.4\;{\mu}g/ml)$, though the antioxidant ability was found not to be potent $(IC_{50}\;97.2\;{\mu}g/ml)$. However, the component eugenol showed potent antioxidant ability but mild cytotoxicity. Methyleugenol with no phenolic OH showed less potent cytotoxic and antioxidative properties than eugenol suggesting that phenolic OH plays an important role for the cytotoxic and antioxidant abilities. The oil-pretreatment prevented lipid peroxidation induced by bromobenzene in the rat. Therefore, it was demonstrated that CS-oil could be a cytotoxic agent with antioxidant properties.

• IMMUNE NETWORK
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• v.13 no.4
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• pp.133-140
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• 2013

Since the discovery of the immunomodulation property of mesenchymal stem cells (MSCs) about a decade ago, it has been extensively investigated whether MSCs can be used for the treatment of immune-related diseases, such as graft versus-host disease (GvHD). However, how to evaluate the efficacy of human MSCs for the clinical trial is still unclear. We used an MHC-mismatched model of GvHD (B6 into BALB/c). Surprisingly, the administration of the human MSCs (hMSCs) could reduce the GvHD-related mortality of the mouse recipients and xenogeneically inhibit mouse T-cell proliferation and $IFN-{\gamma}$ production in vitro. We recently established a new protocol for the isolation of a homogeneous population of MSCs called subfractionation culturing methods (SCM), and established a library of clonal MSC lines. Therefore, we also investigated whether MSCs isolated by the conventional gradient centrifugation method (GCM) and SCM show different efficacy in vivo. Intriguingly, clonal hMSCs (hcMSCs) isolated by SCM showed better efficacy than hMSCs isolated by GCM. Based on these results, the MHC-mismatched model of GvHD may be useful for evaluating the efficacy of human MSCs before the clinical trial. The results of this study suggest that different MSC lines may show different efficacy in vivo and in vitro.

• Biomolecules & Therapeutics
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• v.11 no.2
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• pp.85-90
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• 2003

Microtubule-binding molecules have been developed as anti-cancer agents to overcome the toxicities of current chemotherapeutics and also have potential for use as anti-angiogenic agents. In this work, we examined the effect of novel triazine compounds, Tubulyzines (microTUBUle LYsing triaZINE), derived from the orthogonal synthesis of a triazine library, on endothelial progenitor cell differentiation. When mononuclear cells isolated from human cord blood were cultured on fibronectin-coated plates for 7 days, all the Tubulyzine compounds A, B, and C (TA, TB, and TC) tested decreased the number of adherent cells in a dose-dependent manner in a coo. centration ranges of 2-5 to $80\mu\textrm{M}$. TA ($IC_{50}$=$20\mu\textrm{M}$) showed slightly more potent activity than TB and TC. Adherent cells treated with TA also exhibited a lower level of ability to ac-LDL uptake, with low ratios of positive cells out of total adherent cells, in a dose-dependent manner and weak expression of endothelial lineage markers, KDR, CD31, and vWF at $20\mu\textrm{M}$. Therefore, these results suggest that tubulyzine A (TA) can be effectively used for the inhibition of new vessel growth by inhibiting differentiation of endothelial progenitor cells.