• 정보관리학회지
• /
• 제31권3호
• /
• pp.29-63
• /
• 2014

본 연구에서는 Y세대의 특징을 밝히고 Y세대가 요구하는 차세대디지털도서관서비스를 도출하고자 하였으며, 이들의 요구가 베이비붐세대와 어느 정도 차이를 보이는지를 비교하고자 하였다. 연구결과, 첫째, Y세대가 가장 많이 이용하는 디지털기기는 휴대폰 또는 스마트폰으로 나타났고, 다음으로 데스크탑 PC, 노트북 PC, 디지털 카메라 순으로 나타났으며, 사용비율에 있어서 약간의 차이는 있지만 그 순위는 베이비붐세대와 거의 유사하게 나타났다. 둘째, 이용하는 디지털서비스에 있어서 Y세대와 베이비붐세대는 상당한 차이를 보이고 있는 것으로 분석되었으며, Y세대는 인터넷 포털을 가장 많이 이용하고 베이비붐세대는 이메일서비스를 가장 많이 이용하는 것으로 나타났다. 셋째, Y세대와 베이비붐세대가 차세대디지털도서관에 요구하는 서비스를 클라우드서비스, 무한창조공간, 빅데이터, 증강현실, 구글글래스, 상황인식기술, 시맨틱서비스, SNS서비스, 디지털교과서서비스, RFID 및 QRCode 서비스, 도서관공간구성, 최첨단디스플레이기술, 기타 획기적인 서비스로 구분하여 조사한 결과, Y세대가 가장 높은 요구도를 보인 서비스는 빅데이터서비스였고, 베이비붐세대는 디지털교과서서비스였다.

• 식물조직배양학회지
• /
• 제26권2호
• /
• pp.121-126
• /
• 1999

참나물 (Pimpinella brachycarpa)의 엽병 (petiole)절편체로부터 캘러스가 MS배지 (0.5mg/L 2,4-D와 0.1mg/L BAP)에서 유도되었으며 이들 캘러스로부터 치밀하게 배열된 세포집단(cell cluster)을 선발하여 현탁배양하였다. 이들 현탁배양세포들은 0.1 mg/L NAA가 포함된 MS고체배지에 배양되어 배발생 (embryogenic) 캘러스로 성장하였다. 배발생캘러스는 연한 노란색을 띠며 체세포배로 분화되었으며 이들 체세포배는 MS액체배지에서 발아되어 식물체로 성장하였다. 참나물 현탁배양세포 유래 배발생캘러스로부터 분리한 mRNA로부터 cDNA library를 합성하여 PCR을 수행한 결과 제조된 library의 삽입절편의 크기가 대부분 500bp이상임을 확인하였다. 이들 cDNA library로부터 전체 1.5 $\times$$10^{6}$개의 plaque를 혼성화하여 일차의 screening을 통해 19개의 cDNA clone을, 이차의 screening을 통해 5개의 cDNA clone을 얻었으며 이중 4개의 cDNA clone은 참나물 shoot의 HD-Zip 유전자인 Phz4 유전자와 동일한 약 1.4 kb 정도인 것으로 나타났으나, 1개의 cDNA clone, Phc5는 약 1.5kb정도의 크기를 나타내었다. 1.5kb인 Phc5는 Phz4유전자의 5'쪽으로 163bp의 염기가 추가로 발견되어 총 1,531 bp에 해당하였으며 18개의 polyA tail을 가지고 있었다. Phc5는 284번째에 ATG개시코돈이 있고 302개의 아미노산을 암호화하는 906개의 단백질 암호화 부위와 Homeodomain을 갖고 있었다. Phc5로부터 추정된 단백질은 기존 전사조절자에서 많이 보고된 HD의 구조적 특징을 갖고 있었다.

• Parasites, Hosts and Diseases
• /
• 제44권4호
• /
• pp.303-312
• /
• 2006

Interactions between GRA proteins of dense granules in Toxoplasma gondii and host cell proteins were analyzed by yeast two-hybrid technique. The cMyc-GRA fusion proteins expressed from pGBKT7 plasmid in Y187 yeast were bound to host cell proteins from pGADT7-Rec-HeLa cDNA library transformed to AH109 yeast by mating method. By the selection procedures, a total of 939 colonies of the SD/-AHLT culture, 348 colonies of the $X-\alpha-gal$ positive and PCR, 157 colonies of the $X-\beta-gal$ assay were chosen for sequencing the cDNA and finally 90 colonies containing ORF were selected to analyze the interactions. GRA proteins interacted with a variety of host cell proteins such as enzymes, structural and functional proteins of organellar proteins of broad spectrum. Several specific bindings of each GRA protein to host proteins were discussed presumptively the role of GRA proteins after secreting into the parasitophorous vacuoles (PV) and the PV membrane in the parasitism of this parasite.

• BMB Reports
• /
• 제45권6호
• /
• pp.365-370
• /
• 2012

Hepatitis B virus (HBV) DNA is often integrated into hepatocellular carcinoma (HCC). Although the relationship between HBV integration and HCC development has been widely studied, the role of HBV integration in HCC development is still not completely understood. In the present study, we constructed a pooled BAC library of 9 established cell lines derived from HCC patients with HBV infections. By amplifying viral genes and superpooling of BAC clones, we identified 2 clones harboring integrated HBV DNA. Screening of host-virus junctions by repeated sequencing revealed an HBV DNA integration site on chromosome 11q13 in the SNU-886 cell line. The structure and rearrangement of integrated HBV DNA were extensively analyzed. An inverted duplicated structure, with fusion of at least 2 HBV DNA molecules in opposite orientations, was identified in the region. The gene expression of cancer-related genes increased near the viral integration site in HCC cell line SNU-886.

• 한국동물번식학회:학술대회논문집
• /
• 한국동물번식학회 2003년도 학술발표대회 발표논문초록집
• /
• pp.97-97
• /
• 2003

For many animals the centrosome consists of a pair of centrioles and surrounding pericentriolar materials (PCMs). PCMs have been known to play roles during cell division. It is known that centrioles are necessary to assemble centrosomal components. However, many types of oocytes undergo meiosis without centrioles. It is known that in nonmurine mammalian species, the sperm introduces an intact proximal centriole unlike sea urchin where two centrioles are introduced. In case of mouse sperm, the presence of centrosome is not clear In this study, a monoclonal antibody was developed to investigate centrosome during mouse germ cell and early embryo development. Results of immunostaining and Western blotting in CHO cells suggest that the monoclonal antibody recognizes a nuclear and centrosomal protein, thus called NCP. The NCP monoclonal antibody was used to screen a cDNA expression library prepared from 12.5 mouse brain to isolate NCP gene. Nucleotide size of NCP gene obtained from immunoscreening was about 5.5kb. It is determined that the NCP may be closely related with pericentriolar material -1 gene (Pcm-1) from the result of sequencing analysis. The molecular weight, 66kDa, calculated by known DNA sequence in database is consistent with that of detected from Western blotting using CHO cell lysates. Therefore, it is assumed that NCP may be alternative splicing form of Pcm-1 of which molecular weight is 228kDa. In mouse oocytes, NCP was distributed in nucleus as in CHO cells. It was shown that the NCP was localized around neck region, probably the centrosome in mouse neck region. Interestingly, dramatic change in distribution of NCP was also shown in male germ cell development. Finally, we observed the cellular distribution of NCP during early embryo development. NCP was detected in nucleus as well as centrosome foci. It is suggested that the centrioles reassembly we occurring in blastocysts and then affects the distribution of NCP.

• 대한전자공학회논문지SD
• /
• 제49권3호
• /
• pp.8-14
• /
• 2012

이 논문에서는 $Radix-4^2$알고리즘을 사용한 저면적 FFT 구조를 제안한다. 큰 point의 FFT는 여러 개의 직렬연결 스테이지로 구성되는데, $Radix-4^2$알고리즘을 사용하면 매 2 스테이지마다 곱셈 종류의 수가 3인 스테이지가 생긴다. 이 사실을 이용하여 곱셈 연산 종류의 수가 3인 스테이지의 구현 면적을 줄이는 구조를 제안하였다. 예를 들면 4096-point FFT는 6개의 스테이지로 구성되는데 $Radix-4^2$ 알고리즘을 사용하면 3개의 스테이지가 곱셈연산 종류의 수가 3이다. 이 3개의 스테이지의 곱셈 연산 하드웨어는 CSD(Canonic Signed Digit) 계수 방식과 CSS(Common Sub-expression Sharing) 기술을 사용하여 구현면적 감소시킬 수 있었다. 제안된 방식을 사용하여 256-point FFT 구조를 설계하여 Verilog-HDL 코딩하였다. 또한 tsmc $0.18{\mu}m$ CMOS 라이브러리를 사용하여 합성하여 구현한 결과 $1.971mm^2$의 cell area를 얻었다. 이와 같은 합성 결과는 기존 구조와 비교하여 약 23%의 cell area 감소 효과를 보였다.

• Asian Pacific Journal of Cancer Prevention
• /
• 제15권18호
• /
• pp.7785-7792
• /
• 2014

Background: Valproic acid (VPA) is a potent anticancer and antiangiogenic agent. However, design and synthesis of chemical derivatives with improved antiangiogenic and anticancer activities are still necessary. In this study a library of novel derivatives of VPA was synthesized and tested. Methods: A human liver cancer cell line (HepG2) and a human normal embryonic kidney cell line (HEK 293) were exposed to various concentrations of VPA derivatives for 24 hours and cell viability was checked by MTT colorimetric assay. Anti-angiogenic properties were evaluated in transgenic zebrafish embryos. Results: N-valproylglycine derivatives suppressed survival almost 70% (p value 0.001) in HepG2 cells but only 10-12% in HEK 293 cells (p value 0.133). They also suppressed angiogenic blood vessel formation by 80% when used between $2-20{\mu}M$ in zebrafish embryos. Valproic acid hydrazides showed moderate level of anticancer activity by affecting 30-50% (p value 0.001) of cell viability in HepG2 cells and 8-10% in HEK293 cells (p value 0.034). Conclusion: The majority of compounds in this study showed potent and stronger antiangiogenic and anticancer activity than VPA. They proved selectively toxic to cancer cells and safer for normal cells. Moreover, these compounds inhibited developmental angiogenesis in zebrafish embryos. Based on the fact that liver is a highly vascularized organ, in case of liver carcinoma these compounds have the potential to target the pathological angiogenesis and could be an effective strategy to treat hepatocellular carcinoma.

• BMB Reports
• /
• 제34권4호
• /
• pp.316-321
• /
• 2001

Dehydroascorbate (DHA) reductase (DHAR, EC 1.8.5.1) catalyzes the reduction of DHA to reduced ascorbate (AsA) using glutathione (GSH) as the electron donor in order to maintain an appropriate level of ascorbate in plant cells. To analyze the physiological role of DHAR in environmental stress adaptation, we developed transgenic tobacco (Nicotiana tabacum cv. Xanthi) plants that express a human DHAR gene isolated from the human fetal liver cDNA library in the chloroplasts. We also investigated the DHAR activity, levels of ascorbate, and GSH. Two transgenic plants were successfully developed by Agrobacterium-mediated transformation and were confirmed by PCR and Southern blot analysis. DHAR activity and AsA content in mature leaves of transgenic plants were approximately 1.41 and 1.95 times higher than in the non-transgenic (NT) plants, respectively In addition, the content of oxidized glutathione (GSSG) in transgenic plants was approximately 2.95 times higher than in the NT plants. The ratios of AsA to DHA and GSSG to GSH were changed by overexpression of DHAR, as expected, even though the total content of ascorbate and glutathione was not significantly changed. When tobacco leaf discs were subjected to methyl viologen at $5\;{\mu}M$, $T_0$ transgenic plants showed about a 50% reduction in membrane damage compared to the NT plants.

• Asian-Australasian Journal of Animal Sciences
• /
• 제17권12호
• /
• pp.1641-1646
• /
• 2004

The objective of this study was to generate transgenic mice expressing human resistin gene by using the tetraploidembryonic stem (ES) cell complementation method. Human resistin gene was amplified from human fetal liver cDNA library by PCR, cloned into $pCR^{(R)}$ 2.1 $TOPO^{(R)}$ vector and constructed in pCMV-Tag4C vector. Mammalian expression plasmid containing human resistin was transfected into D3-GL ES cells by Lipofectamine 2,000, and then after 10-12 days of transfection, the human resistin-expressing cells were selected with G418. In order to produce tetraploid embryos, blastomeres of diploid embryos at the two-cell stage were fused with two times of electric pulse using 60 V 30 $\mu$sec (fusion rate: 2,114/2,256, 93.5%) and cultured up to the blastocyst stage (development rate: 1,862/2,114, 94.6%). The selected 15-20 ES cells were injected into tetraploid blastocysts, and then transferred into the uteri of E 2.5 d pseudopregnant recipient mice. To investigate the gestation progress, two E 19.5 mused fetuses were recovered by Cesarean section of which one fetus was confirmed to contain human resistin gene by genomic DNA-PCR. Therefore, our findings demonstrate that tetraploid-ES mouse technology can be considered as a useful tool to produce transgenic mice for the rapid analysis of gene function in vivo.

• BMB Reports
• /
• 제43권12호
• /
• pp.789-794
• /
• 2010

A novel gene, designated mgt-6, containing four splicing variants, was isolated from a gene trap clone library of C3H/10T1/2 cells transfected with retroviral promoterless gene-trap vector, ROSAFARY. The transcript variants were differentially expressed in murine tissues and cell lines and differentially responded to diverse stimuli including TGF-${\beta}1$ and mitogen-activated protein kinase (MAPK) inhibitors. The mgt-6 gene encoded a protein of 37 or 11 amino acid residuals with cytoplasmic distribution. However, when C3H/10T1/2 cells were treated with 5-azacytidine, the protein translocated into cell nucleus as indicated by fused LacZ or C-terminally tagged EGFP. Our preliminary results suggest that further study on the role of mgt-6 gene in cell transformation and differentiation may be of significance.