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http://dx.doi.org/10.5483/BMBRep.2010.43.12.789

Cloning and characterization of a novel gene with alternative splicing in murine mesenchymal stem cell line C3H/10T1/2 by gene trap screening  

Wang, Mingke (Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, College of Preventive Medicine, Third Military Medical University)
Sun, Huiqin (Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, College of Preventive Medicine, Third Military Medical University)
Jiang, Fan (Department of Chemical Defense and Toxicology, College of Preventive Medicine, Third Military Medical University)
Han, Jing (Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, College of Preventive Medicine, Third Military Medical University)
Ye, Feng (Department of Chemical Defense and Toxicology, College of Preventive Medicine, Third Military Medical University)
Wang, Tao (Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, College of Preventive Medicine, Third Military Medical University)
Su, Yongping (Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, College of Preventive Medicine, Third Military Medical University)
Zou, Zhongmin (Department of Chemical Defense and Toxicology, College of Preventive Medicine, Third Military Medical University)
Publication Information
BMB Reports / v.43, no.12, 2010 , pp. 789-794 More about this Journal
Abstract
A novel gene, designated mgt-6, containing four splicing variants, was isolated from a gene trap clone library of C3H/10T1/2 cells transfected with retroviral promoterless gene-trap vector, ROSAFARY. The transcript variants were differentially expressed in murine tissues and cell lines and differentially responded to diverse stimuli including TGF-${\beta}1$ and mitogen-activated protein kinase (MAPK) inhibitors. The mgt-6 gene encoded a protein of 37 or 11 amino acid residuals with cytoplasmic distribution. However, when C3H/10T1/2 cells were treated with 5-azacytidine, the protein translocated into cell nucleus as indicated by fused LacZ or C-terminally tagged EGFP. Our preliminary results suggest that further study on the role of mgt-6 gene in cell transformation and differentiation may be of significance.
Keywords
C3H/10T1/2 cells; Expression pattern; Gene trapping; Nuclear translocation; Splicing variants;
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