• Journal of Microbiology
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• 제34권4호
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• pp.311-315
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• 1996

The retroviral Gag polyprotein directs the assembly of virion particles and plays an important role in some events after entry into a host cell. The Gag polyprotein of a virus mixture is responsible for inducing murine acquired immunodeficiency syndrome (MAIDS) when injected into susceptible strains of mice. In order to identify the host cellular proteins which interact with the MAIDS virus Gag proteins and possibly mediate the function of the Gag proteins, mouse T-cell leukemic cDNA expression library was screened using the yeast GAL4 two hybrid system. Of 11 individual positive clones, the clone Y1 was selected for the study of protein-protein interaction. Its DNA sequence revealed that it was an exact match to the murine SH3 domain-containing protein SH3P8. It is expressed as 2.4 kbp transcripts in testis at higher levels and in various tissues tested at lower levels. Glutathione S-transferase-Y1 fusion protein binds tightly to $Pr60^{def-gag}$ as well as $Pr65^{eco-gag}$.

• 한국통신학회논문지
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• 제23권6호
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• pp.1503-1513
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• 1998

This paper is related to a performance analysis of B-NT system, which is essential compositional equipment of B-ISDN access network. A simulator enabling performance analysis according to the change of network configuration topology and the change of user traffic is developed in this study. The developed B-NT, system simulator consists of graphic user interface module, simulation program automatic generator module, and B-NT system model library module. As examples of the results of performance analysis using the simulator, end-to-end user cell transmission delay time, queueing delay time in each system, and cell loss rate in the head node switch are presented. The simulator developed in this paper can be utilized in determining the network topology of B-NT system.

• 전자공학회논문지A
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• 제30A권11호
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• pp.132-139
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• 1993

This paper presents a new performance-driven multi-levelizer which transforms a two-level description into a boolean network of the multilevel structure satisfied with user's costraints, such as chip area, the number of wires and literals, maximum delay, function level, fanin, fanout, etc.. The performance of circuits is estimated by reference to the informations in cell library through the cell mapping phase, and multi-levelization of circuits is constructed by the decomposition using the kernel and factoring concepts. Here, the saving cost of a common subexpression is defined to the sum of area and delay saved, when it is substituted. The experiments with MCNC benchmarks show the efficiency of the proposed method.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1998년도 추계학술대회 논문집 학회본부 B
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• pp.612-614
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• 1998

As chip feature size decrease, interconnect delay gains more importance. A accurate timing analysis required to estimate interconnect delay as well as cell delay. In this paper, we present a timing-level delay calculation tool of which the accuracy is bounded within 10% of SPICE results. This delay calculation tool generates delay values in SDF(Standard Delay Format) for parasitic data extracted in SPEF(Standard Parasitic Exchange Format). The efficiency of the tool is easily seen because it uses AWE(Asymptotic Waveform Evaluation) algorithm for interconnect delay calculation, and precharacterized library and effective capacitance model for cell delay calculation.

• Journal of Microbiology and Biotechnology
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• 제18권5호
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• pp.926-932
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• 2008

Human heavy chain (H-) and light chain (L-) ferritins were amplified from a human cDNA library. Each ferritin gene was inserted downstream of the T7 promoter of bacterial expression vectors, and two types of coexpression vectors were constructed. The expression levels of recombinant ferritins ranged about 26-36% of whole-cell protein. H-ferritin exhibited a lower expression ratio compared with L-ferritin, by a coexpression system. However, the coexpression of HL-ferritins was significantly increased above the expression ratio of H-ferritin by cultivation without IPTG induction overnight. Purified recombinant H-, L-, HL-, and LH-ferritins were shown to be homo- and heteropolymeric high molecular complexes and it was indicated that their assembled subunits would be able to work functionally in the cell. Thus, these results indicate an improvement in the expression strategy of H-ferritin for heteropolymeric production and studies of ferritin assembly in Escherichia coli.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2008년 영문 학술대회
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• pp.597-601
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• 2008

Three-dimensional structures of detonation wave propagating in circular tube were investigated. Inviscid fluid dynamics equations coupled with a conservation equation of reaction progress variable were analyzed by a MUSCL-type TVD scheme and four stage Runge-Kutta time integration. Variable-$\gamma$ formulation was used to account for the variable properties between unburned and burned states and the chemical reaction was modeled by using a simplified one-step irreversible kinetics model. The computational code was parallelized based on domain decomposition technique using MPI-II message passing library. The computations were carried out using a home made Windows based PC cluster having 160 AMD AthloxXP and Athlon64 processor. The computational domain consisted of through a roundshaped tube with wall conditions. As an initial condition, analytical ZND solution was distributed over the computational domain with disturbances. The disturbances has circumferential large gradient. The unsteady computational results in three-dimension show the detailed mechanisms of multi-cell mode of detonation wave instabilities resulting diamond shape in smoked-foil record.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.326.1-326.1
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• 2002

We applied serological analysis of cDNA expression library technique to identify cancer-associated genes. We screened cDNA expression libraries of human testis and gastric cancer cell lines with sera of patients with gastric cancers. We identified a gene whose expression is testis-specific among normal tissues. We cloned and characterized this novel gene. It contains D-E-A-D box domain and encodes a putative protein of 630 amino acids with possible helicase activity. It showed wide expression in various cancer tissues and cancer cell lines. (omitted)

• 한국발생생물학회:학술대회논문집
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• 한국발생생물학회 2003년도 제3회 국제심포지움 및 학술대회
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• pp.121-121
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• 2003

The standard protocol for the production of transgenic mouse from ES-injected embryo has to process via chimera producing and several times breeding steps, In contrast, tetraploid-ES cell complementation method allows the immediate generation of targeted murine mutants from genetically modified ES cell clones. The advantage of this advanced technique is a simple and efficient without chimeric intermediates. Recently, this method has been significantly improved through the discovery that ES cells derived from hybrid strains support the development of viable ES mice more efficiently than inbred ES cells do. Therefore, the objective of this study was to generate transgenic mice overexpressing human resistin gene by using tetrapioid-ES cell complementation method. Human resistin gene was amplified from human fetal liver cDNA library by PCR and cloned into pCR 2.1 TOPO T-vector and constructed in pCMV-Tag4C vector. Human resistin mammalian expression plasmid was transfected into D3-GL ES cells by lipofectamine 2000, and then after 8~10 days of transfection, the human resistin-expressing cells were selected with G418. In order to produce tetraploid embryos, blastomeres of diploid embryos at the two-cell stage were fused with two times of electric pulse using 60 V 30 $\mu$sec. (fusion rate : 93.5%) and cultured upto the blastocyst stage (development rate : 94.6%). The 15~20 previously G418-selected ES cells were injected into tetraploid blastocysts, and then transferred into the uterus of E2.5d pseudopregnant recipient mice. To investigate the gestation progress, two El9.5d fetus were recovered by Casarean section and one fetus was confirmed to contain human resistin gene by genomic DNA-PCR. Therefore, this finding demonstrates that tetraploid-ES mouse technology can be considered as a useful tool to produce transgenic mouse for the rapid analysis of gene function in vivo.

• 한국통신학회논문지
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• 제36권11C호
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• pp.662-672
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• 2011

본 논문은 새로운 경계선 보존 알고리즘을 이용하여 블록화 현상을 제거하는 디블로킹 필터와 HD해상도의 실시간 영상처리가 가능한 디블로킹 필터의 VLSI구조를 제안한다. 기존의 블록 분류 기반의 접근 방법과 달리 제안된 알고리즘은 픽셀 분류 기반 접근을 사용한다. 또한 제안된 경계선 보존 맵은 픽셀을 경계선 영역과 평탄 영역으로 분류하며, 블록화 현상 제거에 사용되는 오프셋 필터와 경계선 보존 필터의 기반이 된다. 이를 바탕으로 제안된 디블로킹 필터의 VLSI구조는 고연산량 처리를 위하여 블록 전체에 파이프라인 기법을 적용하였다. 또한 블록 버퍼를 위한 메모리 절감 구조는 메모리의 사용을 최적화 시킨다. 본 필터는 VHDL을 이용한 설계를 통하여 CycloneII FPGA상에서 구현된 구조의 동작을 검증 후, Synopsys의 Design Compiler와 ANAM 0.25 ${\mu}m$ CMOS cell library로 합성하여 칩으로 구현하였을 때의 성능을 예측하였다. 제안된 알고리즘의 실험 결과는 세밀한 영상성분을 보존하면서 효과적으로 블록화 현상을 제거하며, 픽셀 분류 기반에서 제안된 알고리즘은 블록 분류 기반보다 PSNR 성능이 우수함을 보였다.

• 대한전자공학회논문지SD
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• 제44권12호
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• pp.93-101
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• 2007

작은 면적과 저전력으로의 구현은 다양한 멀티미디어 하드웨어, 특히 모바일 환경에서 매우 중요한 요구사항이다. 본 논문은 작은 면적과 그에 따른 저전력을 목표로 H.264/AVC 인트라 예측기기 하드웨어 구조를 제안한다. 이미지 프레임을 예측하기 위해 하나의 연산기로 모든 모드 결정과 계산들이 순차적으로 수행기고 그들 중 최적의 값을 선택하는 방식이며, 그 결과로 다른 기존의 논문들 보다 더 작은 면적의 결과를 얻을 수 있었다. 제안된 구조는 Altera Excalibur device를 이용하여 검증되었고, 구현된 하드웨어 구조는 Synopsys Design Compiler와 Samsung STD130 0.18um CMOS Standard Cell Library를 이용하여 합성하였다. 합성결과 크기는 11.9k의 하드웨어 로직 게이트와 1078 byte의 내부 SRAM을 사용하고 최대 동작 주파수는 약 107MHz가 되었다. 제안한 구조는 하나의 QCIF($176\times144$ 화소) 영상 프레임을 처리하는데 879,617클록이 소요되며, 이는 QCIF 영상을 초당 121.5프레임으로 처리가 가능하며, 이는 하드웨어 기반의 실시간 H.264/AVC 부호화 시스템에 적합한 구조임을 보여준다.