• 제목/요약/키워드: cell immobilization

검색결과 209건 처리시간 0.022초

형질전환된 식물세포에서 고정화 방법을 통한 hCM-CSF의 생산성 증대 연구 (Enhanced Production of hGM-CSF by Immobilized Transgenic Plant Cell Cultures)

  • 노윤숙;남형진;최홍열;탁사라;김동일
    • KSBB Journal
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    • 제30권2호
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    • pp.82-90
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    • 2015
  • Plant cell immobilization can protect plant cells from shear forces and increase the stability of gene. An additional advantage of immobilization is the easiness for performing continuous culture with cell recycling. Therefore plant cell immobilization can overcome the limitations of plant cell applications. In addition, target protein should be selected from pharmaceutical proteins to get rid of low expression level problem. The enhanced production of human granulocyte-macrophage colony-stimulating factor (hGM-CSF) was investigated in immobilized Nicotiana tabacum suspension cell cultures. When the cells were immobilized in polyurethane foam, specific production of hGM-CSF was higher than that in alginate bead immobilization. Optimum continuous culture condition was the addition of 60 g/L sucrose in growth media with exchanging media every 6 day. Under the same condition, specific hGM-CSF production was 7 times higher in a 500-mL spinner flask than that in 100-mL Erlenmeyer flasks. Therefore, development of an effective immobilization process would be possible when the advantage of easy cell recycling was used. Consequently, enhanced production of target proteins could be possible in immobilized continuous cultures when the advantages of immobilization were applied.

Improvement in microbial stability of photosynthetic bacteria via optimized cell immobilization and lyophilization: Application to the treatment of shrimp aquaculture water

  • Kyoung Sook, Cho;Joong Kyun, Kim
    • 한국해양바이오학회지
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    • 제14권2호
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    • pp.112-123
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    • 2022
  • Photosynthetic bacteria (PSB) play an important role in water purification, and their application is beneficial for sustainable aquaculture. However, maintaining the microbial stability of PSB from subculturing to preservation is a challenging task. Since improvement in the microbial stability of PSB is a crucial parameter, optimized conditions for cell immobilization and lyophilization were investigated. In PSB immobilization, 0.1-M CaCl2 was found to be the most effective divalent metal ion solution in terms of cost-effectiveness, resulting in beads with a 4-mm diameter and high loading (1.91×109 CFU/mL) of viable cells. Maintenance of cell viability, external appearance, and color of PSB beads was best in 3.5% NaCl during storage. In lyophilization, the addition of skim milk (9%) and dextrose (2%) as cryoprotective additives allowed the highest cell viability. Over an 18-week shrimp breeding period, when optimally manufactured beads and lyophilized powder of PSB were applied to shrimp aquaculture water, NH4+, NO3-, and NO2- were more effectively removed by 55%, 100%, and 100%, respectively, compared to controls. Thus, microbial stability of PSB through optimized cell immobilization and lyophilization was successfully enhanced, enabling a wide application.

청간해울탕(淸肝解鬱湯)이 생취에 Immobilization-Stress 및 Cold-Stress 부하후(負荷後) 혈중(血中)Histamine과 Corticosterone 함량(含量) 및 면역능(免疫能)에 미치는 영향(影響) (Effects of Chungganhaewooltang on Serum Levels of Histamine and Corticosterone and Immune Response after Immobilization-Stress or Cold-Stress in Mice)

  • 강복환;정우석;김송백;유심근
    • 대한한방부인과학회지
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    • 제18권4호
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    • pp.85-105
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    • 2005
  • Purpose : Investigate the effects of Chungganhaewooltang(CHT) on immobilization-stress or cold-stress in C576BL/6J mice. Methods : Male C57BL/6J 30 mice of weighting 18${\pm}$2g, were divided into sixs groups including the immobilization-stress group(5heads), after immobilization-stress CHT oral administration(500mg/kg) groups(5heads), cold-stress group(5heads) and after cold-stress CHT oral administration(500mg/kg) groups(5heads). then we observed changes in the serum histamine and corticosterone level and changes immune system Results : Immobilization-stress or cold-stress increased the serum level of histamine and corticosterone. CHT decreased the serum level of histamine and corticosterone increased by cold-stress. CHT inhibited the release of histamine from mast cells at the concentration of 0.1 mg/ml. In addition, immobilization-stress or cold-stress decreased the cell viability of murine thymocytes and splenocytes. CHT increased the cell viability of thymocytes decreased by immobilization-stress or cold-stress, but did not affect the cell viability of splenocytes decreased by immobilization-stress or cold-stress. Also immobilization-stress or cold-stress increased DNA fragmentation of thymocytes and splenocytes. CHT decreased DNA fragmentation of thymocytes increased by immobilization-stress or cold-stress, but did not affect DNA fragmentation of splenocytes increased by immobilization-stress or cold-stress. Immobilization-stress increased the population of thymic $CD4^+$ cells. CHT decreased the population of thymic $CD4^+$ cells increased by immobolization-stress. Immobilization-stress or cold-stress decreased the population of $B220^+$ cells and increased the population of $thy1^+$ cells. CHT decreased the population of $thy1^+$ cells increased by immobilization-stress or cold-stress. Immobilization-stress or cold-stress increased the population of splenic $CD4^+$ cells and $CD8^+$ cells. CHT decreased the population of splenic $CD4^+$ cells increased by immobolization-stress or cold-stress. Immobilization-stress or cold-stress decreased the production of ${\gamma}-interferon$(IFN) interleukin(IL)-2 and IL-4. CHT enhanced the production of ${\gamma}-IFN$ decreased by immobilization-stress or cold-stress but did not affect the production of IL-2 and IL-4 decreased by immobilization-stress or cold-stress. Furthermore, Immobilization- stress or cold-stress decreased the phagocytic activity of peritoneal macrophages and the production of nitric oxide. CHT enhanced the phagocytic activity and nitric oxide production decreased by cold-stress. Conclusion : CHT may be useful for the prevention and treatment of stress via suppression of serum histamine and corticosterone level and enhancement of immune response.

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Immobilization cell을 이용한 도공액 특성 연구 (Investigation on the Rheological Properties of Coating Colors by immobilization cell)

  • 박시한;김경동;김송주;허용성
    • 한국펄프종이공학회:학술대회논문집
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    • 한국펄프종이공학회 2003년도 추계학술발표논문집
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    • pp.139-147
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    • 2003
  • Coater runnability of the paper coating and final product properties can be affected by the immobilization of coating color and the dewatering into the base paper. During the dewatering, the rheological properties and solids content of a coating are dramatically changed. For the purpose of obtaining better coater runnability and high quality of coater paper many papermakers are trying to improve the water retention of paper coatings by using some additives such as thickener and co-binder. In this study, we tried to investigate the rheological properties and the immobilization point of coatings with immobilization cell during the dewatering of coatings.

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가미귀비탕(加味歸脾湯)이 생쥐에 Immobilization Stress 부하후(負荷後) 혈중(血中) Corticosterone과 Histamine 함량(含量) 및 면역능(免疫能) 변화(變化)에 미치는 영향(影響) (Effects of Kamikwibitang on Serum Levels of Corticosterone and Histamine and Immune Response after Immobilization Stress in Mice)

  • 위석;이승준;유심근
    • 대한한방부인과학회지
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    • 제18권1호
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    • pp.94-110
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    • 2005
  • The purpose of this research was to investigate the effects of Kamikwibitang water extract (KKT) on immobilization stress in C57BL/6J mice. KKT decreased the serum level of histamine and corticosterone increased by immobilization stress. In addition, KKT decreased the cell viability of thymocytes and enhanced the cell viability of splenocytes decreased by immobilization stress. Also, KKT decreased the viability of thymocytes and splenocytes in vitro. KKT decreased DNA fragmentation of splenocytes increased by immobilization stress. KKT decreased the population of thymic $CD4^+CD8^-$ cells increased by immobilization stress, and did not affect the population of $B220^+$ cells and the population of $Thy1^+$ cells changed by immobilization stress and enhanced the population of splenic $CD4^-CD8^+$ cells increased by immobilization stress. KKT enhanced the production of ${\gamma}-interferon$ and did not affect the production of interleukin-2 and interleukin-4 decreased by immobilization stress. Also, KKT decreased the phagocytic activity and the level of nitric oxide decreased by immobilization stress. These results indicate that KKT may be useful for the prevention and treatment of stress via suppression of serum histamine and corticosterone level and enhancement of specific-immune response.

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귀비탕이 C57BL/6 Mouse에 Stress 부하 후 특이적 면역반응에 미치는 영향 (Effects of Kwibitang on the Specific Immune Response after Immobilization Stress in C57BL/6 Mice)

  • 이택렬;한미숙;오찬호;은재순
    • 동의생리병리학회지
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    • 제17권5호
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    • pp.1208-1216
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    • 2003
  • Stress is known to influence the immune function via an effect on the central nervous system. To investigated the effects of Kwibitang water extract (KBT) on the specific immune response in C57BL/6 mice stressed by immobilization, we evaluated the changes in the cell viability, DNA fragmentation and subpopulation of thymocytes and splenocytes. KBT enhanced the cell viability of thymocytes and splenocytes decreased by immobilization stress. Also, KBT decreased DNA fragmentation of thymocytes and splenocytes increased by immobilization stress. KBT decreased the population of CD4/sup +/ cells and CD8/sup +/ cells in thymocytes and Thy1/sup +/ cells in splenocytes increased by immobolization stress, but increased the population of B220/sup +/ cells decreased by immobilization stress. In addition, KBT enhanced the production of ν-interferon and IL-2 decreased by immobilization stress. These results indicate that KBT may be useful for the prevention and treatment of stress via enhancement of the specific immune response

Immobilization of Penicillium citrinum by Entrapping Cells in Calcium Alginate for the Production of Neo-Fructooligosaccharides

  • Lim, Jung-Soo;Park, Seung-Won;Lee, Jin-Won;Oh, Kyeong-Keon;Kim, Seung-Wook
    • Journal of Microbiology and Biotechnology
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    • 제15권6호
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    • pp.1317-1322
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    • 2005
  • This work describes neo-fructooligosaccharides (neo-FOSs) production using the immobilized mycelia of Penicillium citrinum. Some critical factors were evaluated to optimize maximal production of neo-FOS. Optimal alginate and cell concentrations were determined to be $1.96\%$ alginate and $7.17\%$ cell, respectively, by statistical analysis. The optimal concentration of $CaCl_{2}$, which is related to bead stability, was determined to be 2 M. It was possible to increase the neo-FOS production by adding 15 units of glucose oxidase to the batch reaction. By co-immobilizing cells and glucose oxidase, neoFOS productivity increased $123\%$ compared with the whole-cell immobilization process. Based on the results above, a co-immobilization technique was developed and it can be utilized for large-scale production.

Curdlan과 활성탄을 이용한 미생물 고정화 담체개발 (Development of Supporting Materials with Curdlan and Activated carbon for Microbial Immobiliaztion)

  • 손효진;박양호;권규혁;이중헌
    • KSBB Journal
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    • 제18권3호
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    • pp.243-247
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    • 2003
  • 커들란과 활성탄의 조성을 몇 가지로 나누어서 실험한 결과 커들란 30 g/L과 활성탄 6 g/L의 비로 제조하여 Autoclave ($121^{\circ}C$, 1 atm, 15 min)에서 가열한 담체의 물성이 다공성 특성 및 세포의 고정화 측면에서 우수함을 보였다. 충전층 반응기를 설치하여 담체를 고정시킨 후 미생물 부착능력을 실험한 결과, 철 산화 세균에 의하여 시간이 증가할수록 철 산화 속도가 빨라지고, 이 과정을 주사전자현미경으로 촬영한 결과 시간이 증가할수록 담체에 부착되는 미생물의 농도가 증가함을 확인할 수 있었다. 그리고 본 실험에서 사용된 담체는 환경 친화적인 고분자로 2차적으로 발생할 수 있는 환경문제를 해결 할 수 있으며 기존의 담체에 비교하여 비표면적이 넓고, 인체에 해가 없는 생물고분자 담체이므로 식품용도의 흡착제 등으로도 유용하게 사용될 수 있다.

화간전(化肝煎)이 생쥐의 Immobilization-Stress 및 Cold-Stress에 미치는 영향(影響) (Effects of Hwaganjeon on Immobilization-Stress or Cold-Stress in Mice)

  • 박종문;고정민;안규환;최창민;유심근
    • 대한한방부인과학회지
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    • 제19권4호
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    • pp.93-116
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    • 2006
  • Purpose : This study is to examine the effects of Hwaganjeon water extract(HGJ) on immobilization-stress or cold-stress in BALB/C mice. Methods : We have Hwaganjeon water extract(HGJ) by freeze-dryer & melt it by a saline solution. We feed HGJ 500mg/Kg to 5mice, and add immobilization-stress by putting mice in plastic cylinder 10 hours, and add cold-stress by putting mice in $4^{\circ}C$ cold room 6 hours. Results : 1. HGJ decreased the serum level of histamine and corticosterone increased by immobilization-stress or cold-stress. HGJ inhibited the release of histamine from mast cells at the concentration of 1 mg/ml. 2. HGJ did not affect the cell viability of thymocytes decreased by immobilization-stress or cold-stress, but increased the cell viability of splenocytes decreased by immobilization-stress or cold-stress. 3. HGJ decreased the population of splenic $CD4^{+}$ and $CD8^{+}$cells increased by immobolization-stress or cold-stress. 4. HGJ enhanced the production of ${\gamma}$-interferon(IFN) and interleukin(IL)-2 decreased by immobilization-stress or cold-stress. Conclusion : These results indicate that HGJ may be useful for the prevention and treatment of stress via suppression of serum histamine and corticosterone level and enhancement of immune response.

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Strontium Alginate를 담체로 한 Photobacterium phosphoreum 고정화 조건의 최적화 (Optimization of the Condition of Immobilized Photobacterium phosphoreum with Strontium Alginate)

  • 이홍주;김현숙;정계훈;이은수;전억한
    • 한국미생물·생명공학회지
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    • 제27권2호
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    • pp.136-144
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    • 1999
  • Since the condition of immobilization must be optimized, it is very important to know whether and on how conditions bacterial cells retain their metabolic activity during immobilization process. A bioluminescence intensity had the maximum value when cell concentrations were between 1.0 and 1.2 measured at O.D660. The strontium alginate was used as an immobilization matrix and two independent factors for immobilization of Photobacterium phosphoreum with strontium alginate were optimized with the response surface methodology(RSM) considering degree of bioluminescence. As a result, the optimum concentration for immobilization was found to be 2.4%(w/w) for sodium alginate and 0.31M for strontium chloride, respectively. A dilution was carried out with 2.5%(w/v) NaCl solution that is an optimum environmental condition for growth of P. phosphoreum. Under the such condition of immobilization, hardness could be predicted as 4.66$\times$104N/$m^2$ and it took different time according to the volume of matrix to be immobilized completely.

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