• Korean Journal of Poultry Science
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• v.43 no.4
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• pp.213-218
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• 2016

This study was conducted to investigate the effect of taurine supplementation on heat shock protein 70 and in vitro protein turnover in broiler chicks under chronic heat stress. Chicks were allocated into 3 groups of 10 birds per group; the control group was maintained at a temperature of $24^{\circ}C$ without taurine (CO group), the heat-stressed group maintained at a temperature of $34^{\circ}C$ without taurine (HO group), and heat-stressed group maintained at a temperature of $34^{\circ}C$ with taurine (HT group). The final body and liver weights of broilers in the HO and HT groups were significantly lower than those of broilers in the CO group (P<0.05). However, these parameters of the broilers in the HT group were significantly higher than those of broilers in the HO group (P<0.05). The heat shock protein 70 (hsp70) concentration in the liver of broilers in the HO group was significantly higher than that of broilers in the CO and HT groups, but the hsp70 concentration in the liver of broilers in the HT group was not different from that of broilers in the CO group. Liver homogenates of 21 day-old broilers were incubated at temperatures of $37^{\circ}C$ and $45^{\circ}C$ to prove the effect of high temperature and taurine on total protein syntheses. Neither high temperature nor taurine supplementation affected protein syntheses in liver homogenates of the broilers. However, the more the temperature increased, the more the degradation rates of cytoplasmic protein in liver homogenates increased; however, taurine supplementation had no effects on the protein syntheses in the liver of the broiler. It is possible that taurine indirectly affected protein turnover via various physiological mechanisms.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.44 no.1
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• pp.95-101
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• 2018

The epidermis which is stratified by epithelial tissue renewal based on keratinocyte differentiation protects the organism from various environmental insults by forming a physical barrier. Autophagy is a mechanism which mediates lysosomal delivery and degradation of protein aggregates, damaged organelles and intracellular microorganisms. Recent reports have shown that autophagy has critical roles for proper terminal differentiation to stratum corneum via removing metabolic organelles and nuclei. However, whether increasing autophagy can activate epidermal differentiation is unknown. Here, we screened a library of natural single compounds and discovered that betaine specifically increased the LC3 positive cytosolic punctate vesicles and LC3-I to LC3-II conversion in HaCaT human keratinocyte cell line, indicating increased autophagy flux. mTOR pathway, which negatively regulates autophagy, was not affected by betaine treatment, suggesting betaine-induced autophagy through an mTOR-independent pathway. Betaine-induced autophagy was also observed in primary human keratinocyte and skin equivalent. Furthermore, epidermal thickness was increased in skin equivalent under betaine treatment. Overall, our finding suggests that betaine as a novel regulator of autophagy may induce epidermal turnover and improve the skin barrier abnormality of the aged epidermis.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.39 no.1
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• pp.20-33
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• 2002

Usage of ATM AAL2 packets becomes dominant to increase transmission efficiency of voice traffic in the backbone network. In case of voice service that uses AAL2 mechanism, if resources of network are enough, connection of new call is accepted. However, due to packets generated by the new call, transmission delay of packets from old calls can increase sharply. To control this behavior, in this paper we present an AAL2 buffer management scheme that allocates a virtual buffer to each call and after calculating its propagation delay variation(PDV), decides to drop packets coming from each call according to the PDV value. We show that this packet dropping algorithm can effectively prevent abrupt QoS degradation of old calls. To do this, we analyze AAL2 packet composition process to find a critical factor in the process that influences the end-to-end delay behavior and model the process by K-policy M/D/1 queueing system and MIN(K, Tc)-policy M/D/1 queueing system. From the mathematical model, we derive the probability generating function of AAL2 packets in the buffer and mean waiting time of packets in the AAL2 buffer. Analytical results show that the AAL2 packet dropping algorithm can provide stable AAL2 packetization delay and ATM cell generation time even if the number of voice sources increases dramatically. Finally we compare the analytical result to simulation data obtained by using the COMNET Ⅲ package.

• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.1
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• pp.30-39
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• 2014

HanPoong Leading (HPL)-04 were prepared with different oriental medicines (balk of Kalopanax pictus balk, Chaenomelis Fructus, Angelica gigas root, Zingiber officinale, Raphanus sativus Linne and Saururus chinensis Baill.) to investigate the protective effects of HPL-04 on cartilage degradation in knee osteoarthritis (OA). Rat articular chondrocytes incubated with rhIL-$1{\alpha}$ markedly increased matrix metalloproteinase (MMP)-2 and 9 activities, decreased cell viability and reduced chondrogenic gene expression. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, MMP-2 and 9 activities and real time RT-PCR indicated that HPL-04 counteracted these harmful effects in dose-dependent manner. In addition, for experimental OA in vivo, monosodium iodoacetate (MIA, 0.5 mg/50 ${\mu}L$) was injected into knee joints of rats and administered HPL-04 to rats for 4 consecutive weeks after MIA treatment. The experimental data showed that treatment with HPL-04 significantly prevented of MMP-2 and 9 activities in articular cartilage. Histopathological and micro-CT evaluations of the knee joints also revealed that HPL-04 effectively ameliorated MIA-induced degenerative OA. In conclusion, HPL-04 has potential applicability for the prevention and treatment of degenerative OA.

• World Technopolis Review
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• v.3 no.2
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• pp.89-96
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• 2014

Center for Research of Science and Technology ("PUSPIPTEK") has 460 hectares land area, still maintained as a green area with more than 30% green space. There are 47 centers for research and testing technology, technology-based industries, and as well as public supporting facilities in PUSPIPTEK area. Based on the concepts developed to make this area as an ecological region, PUSPIPTEK can be seen as a model of eco-innovation. The purpose of this research is to develop a model of water, energy and waste management with eco-innovation concept. As a new approach in addressing environmental degradation and maintaining the sustainability of ecosystem, studies related to eco-innovation policy that combines the management of water, energy and waste in the region has not been done. In order to achieve the objectives of the research, a series of techniques for collecting data on PUSPIPTEK existing conditions will be carried out, which includes utilities data (water, electricity, sewage) and master plan of this area. The savings over the implementation of the concept of eco-innovation in water, energy, and waste management were calculated and analyzed using quatitative methods. The amount of cost savings and feasibility were then calculated. Eco innovation in water management among other innovations include the provision of alternative sources of water, overflow of rain water and water environments utilization, and use of gravity to replace the pumping function. Eco-innovation in energy management innovations include the use of LED and solar cell for air conditioning. Eco-innovation in waste management includes methods of composting for organic waste management. The research results: (1) The savings that can be achieved with the implementation of eco innovation in the water management is Rp. 3,032,640 daily, or Rp.1,106,913,600 annually; (2) The savings derived from the implementation of eco innovation through replacement of central AC to AC LiBr Solar Powered will be saved Rp.1,933,992,990 annually and the use of LED lights in the Public street lighting PUSPIPTEK saved Rp.163,454,433 annually; (3) Application of eco innovation in waste management will be able to raise awareness of the environment by sorting organic, inorganic and plastic waste. Composting and plastic waste obtained from the sale revenue of Rp. 44,016,000 per year; (4) Overall, implementation of the eco-innovation system in PUSPIPTEK area can saves Rp. 3,248,377,023 per year, compared to the existing system; and (5)The savings are obtained with implementation of eco-innovation is considered as income. Analysis of the feasibility of the implementation of eco-innovation in water, energy, and waste management in PUSPIPTEK give NPV at a 15% discount factor in Rp. 3,895,228,761; 23.20% of IRR and 4.48 years of PBP. Thus the model of eco-innovation in the area PUSPIPTEK is feasible to implement.

• The Korean Journal of Zoology
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• v.32 no.1
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• pp.55-73
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• 1989

The effect of tryptophan or serotonin on the early stage of chick brain development has been morphologically investigated using an electron microscope. The electron micrographs of neural plate cells of 1-day chick embryo treated with tryptophan or serotonin showed irregularity, evagination and disruption of nuclear membrane and nuclear chromatin condenstation, nucleolar margination and segregation. Hypertrophy of stalks, vesicles and vaculoes were seen and dilated and disrupted rough endoplasmic reticulum and underdeveloped neurotubules were also observed. In mesenchyme cells of tryptophan or serotonin administered 18 hr embryo, irregular nuclear membrane, swollen mitochondria, dilated rough endoplasmic reticulum and very large yolk granules were observed. Furthermore, DNA, RNA and protein contents of the embryos treated with typtophan or serotonin were considerably lower than those of control group. The amount of tubulin of the experimental groups was also greatly lower than that of control, suggesting that the impairment of microtubule formation occurred. Tryptophan or serotonin administration might depress the biosynthesis, of nucleic acid and protein including some enzymes tested. It seems that the serotonin formed from exogeneous tryptophan might inhibit the degradation of yolk granule by feedback regulation mechanism so as to impair microtububle and microvilli formation followed by a malformation of chick embryos.

• Journal of Life Science
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• v.27 no.12
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• pp.1403-1409
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• 2017

In this study, the xylitol dehydrogenase (XYL2) gene was expressed in Saccharomyces cerevisiae as a host cell for ease of use in the degradation of lignocellulosic biomass (xylose). To select suitable expression systems for the S.XYL2 gene from S. cerevisiae and the P.XYL2 gene from Pichia stipitis, $pGMF{\alpha}-S.XYL2$, $pGMF{\alpha}-P.XYL2$, $pAMF{\alpha}-S.XYL2$ and $pAMF{\alpha}-P.XYL2$ plasmids with the GAL10 promoter and ADH1 promoter, respectively, were constructed. The mating factor ${\alpha}$ ($MF{\alpha}$) signal sequence was also connected to each promoter to allow secretion. Each plasmid was transformed into S. cerevisiae $SEY2102{\Delta}trp1$ strain and the xylitol dehydrogenase activity was investigated. The GAL10 promoter proved more suitable than the ADH1 promoter for expression of the XYL2 gene, and the xylitol dehydrogenase activity from P. stipitis was twice that from S. cerevisiae. The xylitol dehydrogenase showed $NAD^+$-dependent activity and about 77% of the recombinant xylitol dehydrogenase was secreted into the periplasmic space of the $SEY2102{\Delta}trp1/pGMF{\alpha}-P.XYL2$ strain. The xylitol dehydrogenase activity was increased by up to 41% when a glucose/xylose mixture was supplied as a carbon source, rather than glucose alone. The expression system and culture conditions optimized in this study resulted in large amounts of xylitol dehydrogenase using S. cerevisiae as the host strain, indicating the potential of this expression system for use in bioethanol production and industrial applications.

• KSBB Journal
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• v.21 no.2
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• pp.133-139
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• 2006

In 'solid-phase' PEGylation, the conjugation reaction occurs as the proteins are attached to a solid matrix, and thus it can have distinct advantages over the conventional, solution-phase process. We report a case study: rhIFN-${\alpha}$-2a was first adsorbed to cation exchange resin and then N-terminally PEGylated by aldehyde mPEG of 5, 10, and 20 kD through reductive alkylation. After the PEGylation, salt gradient elution efficiently recovered the mono-PEGylate in a purified form from the unwanted species such as unmodified IFN, unreacted PEG, and others. The mono-PEGylation and its purification were integrated in a single chromatographic step. Depending on the molecular weight of the mPEG aldehyde used, the mono-PEGylation yield ranged 50-64%. We could overcome the major problems of random, or uncontrollable, multi-PEGylation and the post-PEGylation purification difficulties associated with the solution-phase process. N-terminal sequencing and MALDI-TOF MS confirmed that a PEG molecule was conjugated only to the N-terminus. Compared with the unmodified IFN, the mono-PEGylate showed the reduced anti-viral activity as measured by the cell proliferation assay. The bioactivity was reduced more as the higher molecular weight PEG was conjugated. Immunoreactivity, evaluated indirectly by antibody binding activity using a surface plasmon resonance biosensor, also decreased. Nevertheless, trypsin resistance as well as thermal stability was considerably improved.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.34 no.2A
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• pp.177-183
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• 2009

In this paper, we propose an improved anti-collision algorithm. We have designed an anti-collision unit using this algorithm for the 18000-6 Type C Class 1 Generation 2 standard (Gen2). The Gen2 standard uses a Q-algorithm for incremental method on the Dynamic Slot-Aloha algorithm. It has basically enhanced performance over the Slot-Aloha algorithm. Unfortunately, there are several non-clarified parts: initial $Q_{fp}$ value, weighted C, and the ending point of the algorithm. If an incorrect value is selected, it causes degradation in performance. Thus we propose an improved anti-collision algorithm by clearly defining the vague parts of the existing algorithm. Simulation results showed an improved performance of up to 34.8% using an optimized value of C and the initial $Q_{fp}$ value. With the ending condition, performance is 34.7%. The anti-collision unit is designed using the Verilog HDL. The module was synthesized using Synopsys' Design Compiler and the TSMC $0.2{\mu}m$ standard cell library. The synthesized result yielded 3,847 gates, and was guaranteed under the proposed working frequency of 19.2MHz.

• Nutrition Research and Practice
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• v.12 no.2
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• pp.129-134
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• 2018

BACKGROUND/OBJECTIVES: Although several recent studies have reported the anti-cancer effects of extracts or components of Citrus unshiu peel, which has been used for various purposes in traditional medicine, the molecular mechanisms for their effects remain unclear. In the present study, the anti-cancer activity of a water-soluble extract of C. unshiu peel (WECU) in MDA-MB-231 human breast carcinoma cells at the level of apoptosis induction was investigated. MATERIALS/METHODS: Cytotoxicity was evaluated using the MTT assay. Apoptosis was detected using DAPI staining and flow cytometry analyses. Mitochondrial membrane potential, reactive oxygen species (ROS) assay, caspase activity and Western blotting were used to confirm the basis of apoptosis. RESULTS: The results indicated that WECU-induced apoptosis was related to the activation of caspase-8, and -9, representative initiator caspases of extrinsic and intrinsic apoptosis pathways, respectively, and caspase-3 accompanied by proteolytic degradation of poly(ADP-ribose) polymerase and down-regulation of the inhibitors of apoptosis protein family members. WECU also increased the pro-apoptotic BAX to anti-apoptotic BCL-2 ratio, loss of mitochondrial membrane potential and cytochrome c release from mitochondria to cytoplasm. Furthermore, WECU provoked the generation of ROS, but the reduction of cell viability and induction of apoptosis by WECU were prevented when ROS production was blocked by antioxidant N-acetyl cysteine. CONCLUSIONS: These results suggest that WECU suppressed proliferation of MDA-MB-231 cells by activating extrinsic and intrinsic apoptosis pathways in a ROS-dependent manner.