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http://dx.doi.org/10.3746/jkfn.2014.43.1.030

Effects of HPL-04 on Degenerative Osteoarthritis  

Na, Ji-Young (Dept. of Laboratory Animal Medicine, College of Veterinary Medicine, Chonbuk National University)
Song, Ki-Bbeum (Dept. of Laboratory Animal Medicine, College of Veterinary Medicine, Chonbuk National University)
Kim, Sukho (Dept. of Laboratory Animal Medicine, College of Veterinary Medicine, Chonbuk National University)
Kwon, Young-Bae (Dept. of Pharmacology, Institute for Medical Science, Chonbuk National University)
Kim, Dae-Gi (Dept. of Immunology, Institute for Medical Science, Chonbuk National University)
Lee, Jun-Kyoung (Central Research and Developement, Hanpoong Pharm & Foods)
Jo, Hyoung-Kwon (Central Research and Developement, Hanpoong Pharm & Foods)
Kwon, Jungkee (Dept. of Laboratory Animal Medicine, College of Veterinary Medicine, Chonbuk National University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.43, no.1, 2014 , pp. 30-39 More about this Journal
Abstract
HanPoong Leading (HPL)-04 were prepared with different oriental medicines (balk of Kalopanax pictus balk, Chaenomelis Fructus, Angelica gigas root, Zingiber officinale, Raphanus sativus Linne and Saururus chinensis Baill.) to investigate the protective effects of HPL-04 on cartilage degradation in knee osteoarthritis (OA). Rat articular chondrocytes incubated with rhIL-$1{\alpha}$ markedly increased matrix metalloproteinase (MMP)-2 and 9 activities, decreased cell viability and reduced chondrogenic gene expression. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, MMP-2 and 9 activities and real time RT-PCR indicated that HPL-04 counteracted these harmful effects in dose-dependent manner. In addition, for experimental OA in vivo, monosodium iodoacetate (MIA, 0.5 mg/50 ${\mu}L$) was injected into knee joints of rats and administered HPL-04 to rats for 4 consecutive weeks after MIA treatment. The experimental data showed that treatment with HPL-04 significantly prevented of MMP-2 and 9 activities in articular cartilage. Histopathological and micro-CT evaluations of the knee joints also revealed that HPL-04 effectively ameliorated MIA-induced degenerative OA. In conclusion, HPL-04 has potential applicability for the prevention and treatment of degenerative OA.
Keywords
HPL-04; osteoarthritis; monosodium iodoacetate; chondrocytes; matrix metalloproteinase;
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1 An HJ, Lee CK, Park JH, Kim KH, Lee WR, Park IY, Han SM, Lee KG, Park KK. 2012. Effects of bee venom on papain-induced osteoarthritis in an animal model. Korean J Phamacogn 43: 167-172.   과학기술학회마을
2 Kim TJ. 1996. Korean resources plants. Seoul National University Press, Seoul, Korea. p 169.
3 Guzman RE, Evans MG, Bove S, Morenko B, Kilgore K. 2003. Mono-iodoacetate-induced histologic changes in subchondral bone and articular cartilage of rat femorotibial joints: an animal model of osteoarthritis. Toxicol Pathol 31:619-624.   DOI
4 Bresnihan B. 1999. Pathogenesis of joint damage in rheumatoid arthritis. J Rheumatol 26: 717-719.
5 Nagasa H, Woessner JF. 1999. Matrix metalloproteinases. J Biol Chem 274: 21491-21494.   DOI
6 Halliwell B. 2000. Oral inflammation and reactive species: a missed opportunity? Oral Dis 6: 136-137.
7 van der Kraan PM, Vitters EL, van de Putte LB, van den Berg WB. 1989. Development of osteoarthritis lesions in mice by "metabolic" and "mechanical" alterations in the knee joints. Am J Pathol 135: 1001-1014.
8 Cho HJ, Chang CB, Jung JW, Seong SC, Kim TK. 2009. Prevalence of radiographic knee osteoarthritis in elderly Koreans. Knee Surgery & Related Research 21: 223-231.
9 Kim W, Choi J. 2010. Effects of bee venom and Cervi cornu parvum pharmacoacupuncture in monosodium iodoacetate (MIA)-induced osteoarthritis rat. The Korean Academy of Oriental Rehabilitation Medicine 20: 61-77.
10 Jang JH, Kawakita K, Hahn SK, Park HJ, Lee SD, Kim YS, Takahashi N, Shichidou T, Itoh K, Sumiya E, Furuya E, Yamashita H. 2006. Report: report of the 3rd Japan-Korea workshop on acupuncture and EBM-protocol development for the acupuncture trial on the osteoarthritis of the knee. J Korean Acupuncture & Moxibustion Medicine Society 23: 239-254.   과학기술학회마을
11 Lee KG, Huang DS, Yu YB, Ma JY, Ha HK, Shin HK. 2006. A study on compositions, dosages and usages of Sagunjatang, Samultang, Palmultang, Sipjeondaebotang in literature. J Oriental Medical Classics 19: 219-225.   과학기술학회마을
12 Lee RM, Nam SS, Lee SH, Kim YS. 2009. Research trends in evidence based medicine on acupuncture - Randomized controlled trial -. J Korean Acupuncture & Moxibustion Medicine Society 26: 147-158.   과학기술학회마을
13 The Korean Orthopaedic Association. 1994. Orthopedics. 4th ed. Newest Medicine Company, Seoul, Korea. p 1-910.
14 Jang YO. 2007. Pathogenesis of osteoarthritis. Diagnosis and Treatment 27: 395-399.
15 Jhun HJ, Ahn K, Lee SC. 2010. Estimation of the prevalence of osteoarthritis in Korean adults based on the data from the fourth Korea national health and nutrition examination survey. Anesth Pain Med 5: 201-206.
16 Altman R, Asch E, Bloch D, Bole G, Borenstein D, Brandt K, Christy W, Cooke TD, Greenwald R, Hochberg M, Howell D, Kaplan D, Koopman W, Longley III S, Mankin H, McShane DJ, Medsger Jr T, Meenan R, Mikkelsen W, Moskowitz R, Murphy W, Rothschild B, Segal M, Sokoloff L, Wolfe F. 1986. Development of criteria for the classification and reporting of osteoarthritis: classification of osteoarthritis of the knee. Arthritis Rheum 29: 1039-1049.   DOI   ScienceOn
17 Wenz W, Breusch SJ, Graf J, Stratman U. 2002. Ultrastructural findings after intraarticular application of hyaluronan in a canine model of arthropathy. J Orthop Res 18: 604-612.
18 Janf SK. 2007. The effect on intra-articular administration of hyaluronic acid and growth hormone on arthritis in osteoarthritis rat model. MS Thesis. Dongshin University, Naju, Korea.
19 Das SK, Faroqi A. 2008. Osteoarthritis. Best Pract Res Clin Rheumatol 22: 657-675.   DOI   ScienceOn
20 Yoon CH. 2012. Current clinical practice: osteoarthritis update. Korean J Med 82: 170-174.   DOI
21 Zahmatkash M, Vafaeenasab MR. 2011. Comparing analgesic effects of a topical herbal mixed medicine with salicylate in patients with knee osteoarthritis. Pak J Biol Sci 14: 715-719.   DOI
22 Kobayashi K, Imaizumi R, Sumichika H, Tanaka H, Goda M, Fukunari A, Komatsu H. 2003. Sodium iodoacetate-induced experimental osteoarthritis and associated pain model in rats. J Vet Med Sci 65: 1195-1199.   DOI   ScienceOn
23 Lechner M, Steirer I, Brinkhaus B, Chen Y, Kirst-Dungl C, Koschier A, Grantschacher M, Neumann K, Zauner- Dungl A. 2011. Efficacy of individualized Chinese herbal medication in osteoarthritis of hip and knee: a double-blind, randomized-controlled clinical study. J Altern Complement Med 17: 539-547.   DOI   ScienceOn
24 Jang JK. 1994. JoongKyung collection. Daesung press, Seoul, Korea. p 385.
25 Herbal medical professor coeditorship. 1999. Herbal medicine. YoungLim, Seoul, Korea. p 1-775.
26 Janusz MJ, Hookfin EB, Heitmeyer SA, Woessner JF, Freemont AJ, Hoyland JA, Brown KK, Hsieh LC, Almstead NG, De B, Natchus MG, Pikul S, Taiwo YO. 2001. Moderation of iodoacetate-induced experimental osteoarthritis rats by matrix metalloproteinase inhibitors. Osteoarthritis Cartilage 9: 751-760.   DOI   ScienceOn