• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
• /
• pp.233-236
• /
• 2000

Bacillus thuringiensis (bt)는 생물학적 살충제 시장에서 가장 널리 사용되는 미생물 살충제이다. Bt의 고농도로 세포배양을 하면 생물반응기에서 원하는 생산물의 농도를 이룰 수 있을 뿐만 아니라 생산물의 생성속도도 이룰 수 있다. 이런 목적 하에서 우리는 세라믹 막 모듈을 반응기 내부에 장착한 새로운 형태의 생물반응기를 개발하였다. 이 새로운 형태의 생물반응기에서 포도당 제한 상태하의 운전을 통해서 bt의 세포와 포자 수율을 현저하게 증가시킬 수 있었다. 최대로 $1.2\;{\times}\;10^{10}\;CFU/ml$의 포자 수율을 달성할 수 있었다. 세라믹 막 모듈 생물반응기에서 포도당 제한 상태 하에서 bt의 성장특성을 연구하였다. 포도당 제한 상태에서는 세포의 성장은 선형 (linear) 성장특성을 보였는데 이것은 간단한 비구조적 수학적 모델의 결과와 잘 일치하였다.

• 한국식생활문화학회지
• /
• 제36권6호
• /
• pp.633-639
• /
• 2021

Raphanus sativus var. hortensis f. raphanistroides Makino (Korean wild radish [WR]) are root vegetables belonging to the Brassicaceae family. These radish species mostly grow in sea areas in Asia, where they have been traditionally used as a medicinal food to treat various diseases. To investigate the effect of WR on neuronal cell death in SH-SY5Y cells, beta-amyloid was used to develop the cell death model. WR attenuated neuronal cell death in SH-SY5Y and regulated the mitogen-activated protein kinase (MAPK) signaling. WR extract also inhibited acetylcholinesterase inhibitor activity. Additionally, the WR treatment group ameliorated the behavior of the memory-impaired mice in a scopolamine-induced mouse model. In the behavior test, WR treated mice showed shorter escape latency and swimming distance and improved the platform-crossing number and the swimming time within the target quadrant. Furthermore, WR prevented histological loss of neurons in hippocampal CA1 regions induced by scopolamine. This study shows that WR can prevent memory impairment which may be a crucial way for the prevention and treatment of memory dysfunction and neuronal cell death.

• Tissue Engineering and Regenerative Medicine
• /
• 제15권6호
• /
• pp.721-733
• /
• 2018

BACKGROUND: Because three-dimensional (3D) models more closely mimic native tissues, one of the goals of 3D in vitro tissue models is to aid in the development and toxicity screening of new drug therapies. In this study, a 3D skin wound healing model comprising of a collagen type I construct with fibrin-filled defects was developed. METHODS: Optical imaging was used to measure keratinocyte migration in the presence of fibroblasts over 7 days onto the fibrin-filled defects. Additionally, cell viability and growth of fibroblasts and keratinocytes was measured using the $alamarBlue^{(R)}$ assay and changes in the mechanical stiffness of the 3D construct was monitored using compressive indentation testing. RESULTS: Keratinocyte migration rate was significantly increased in the presence of fibroblasts with the cells reaching the center of the defect as early as day 3 in the co-culture constructs compared to day 7 for the control keratinocyte monoculture constructs. Additionally, constructs with the greatest rate of keratinocyte migration had reduced cell growth. When fibroblasts were cultured alone in the wound healing construct, there was a 1.3 to 3.4-fold increase in cell growth and a 1.2 to 1.4-fold increase in cell growth for keratinocyte monocultures. However, co-culture constructs exhibited no significant growth over 7 days. Finally, mechanical testing showed that fibroblasts and keratinocytes had varying effects on matrix stiffness with fibroblasts degrading the constructs while keratinocytes increased the construct's stiffness. CONCLUSION: This 3D in vitro wound healing model is a step towards developing a mimetic construct that recapitulates the complex microenvironment of healing wounds and could aid in the early studies of novel therapeutics that promote migration and proliferation of epithelial cells.

• The Korean Journal of Physiology and Pharmacology
• /
• 제24권6호
• /
• pp.441-452
• /
• 2020

In vivo animal models are limited in their ability to mimic the extremely complex systems of the human body, and there is increasing disquiet about the ethics of animal research. Many authorities in different geographical areas are considering implementing a ban on animal testing, including testing for cosmetics and pharmaceuticals. Therefore, there is a need for research into systems that can replicate the responses of laboratory animals and simulate environments similar to the human body in a laboratory. An in vitro two-dimensional cell culture model is widely used, because such a system is relatively inexpensive, easy to implement, and can gather considerable amounts of reference data. However, these models lack a real physiological extracellular environment. Recent advances in stem cell biology, tissue engineering, and microfabrication techniques have facilitated the development of various 3D cell culture models. These include multicellular spheroids, organoids, and organs-on-chips, each of which has its own advantages and limitations. Organoids are organ-specific cell clusters created by aggregating cells derived from pluripotent, adult, and cancer stem cells. Patient-derived organoids can be used as models of human disease in a culture dish. Biomimetic organ chips are models that replicate the physiological and mechanical functions of human organs. Many organoids and organ-on-a-chips have been developed for drug screening and testing, so competition for patents between countries is also intensifying. We analyzed the scientific and technological trends underlying these cutting-edge models, which are developed for use as non-animal models for testing safety and efficacy at the nonclinical stages of drug development.

• 한국미생물학회:학술대회논문집
• /
• 한국미생물학회 2008년도 International Meeting of the Microbiological Society of Korea
• /
• pp.171-171
• /
• 2008

We monitored the occurrence of human enteric viruses in urban rivers by cell culture-PCR and RT-nested PCR. Water samples were collected monthly or semimonthly between May 2002 and March 2003 in four urban tributaries. Enteric viruses were detected by RT-nested PCR and cell culture-PCR based on a combination of Buffalo Green monkey kidney (BGMK) and A549 cell lines, followed by phylogenetic analysis of amplicons. By RT-nested PCR analysis, 45 (77.6%), 32 (55.2%), 32 (55.2%), 26 (44.8%), 12 (20.7%), 2 (3.4%), 4 (6.9%), and 4 (6.9%) of 58 samples showed positive results with adenoviruses, enteroviruses, noroviruses (NV) genogroup I (GI) and II (GII), reoviruses, hepatitis A viruses, rotaviruses and sapoviruses, respectively. Adenoviruses were most often detected and only eight (13.8%) samples were negative for adenoviruses and positive for other enteric viruses in the studied sites. Thirty-one (77.5%) of the 40 samples were positive for infectious adenoviruses and/or enteroviruses based on cell culture-PCR, and the frequency of positive samples grown on A549 and BGMK (65.0%) was higher than that grown on BGMK alone (47.5%). The occurrence of each enteric virus, except reoviruses and hepatitis A viruses was not statistically correlated with the water temperature and levels of fecal coliforms according to Binary logistic regression model. By sequence analysis, most strains of adenoviruses and enteroviruses detected in this study are similar to the causative agent of viral diseases in Korea and most NV GI- and GII-grouped strains were closely related to the reference strains from China and Japan, and GII/4-related strains had similar sequences to strains recognized as a worldwide epidemic outbreak. Our results suggested that monitoring human enteric viruses is necessary to improve microbial quality and cell culture-PCR using the combination of A549 and BGMK cells and the adenovirus detection by PCR could be useful for monitoring viral contamination in the aquatic environment.

• 반도체디스플레이기술학회지
• /
• 제20권1호
• /
• pp.99-103
• /
• 2021

Warpage defect detecting of scaffold is very important in biosensor production. Because warpaged scaffold cause problem in cell culture. Currently, there is no detection equipment to warpaged scaffold. In this paper, we produced detection model for shape warpage detection using deep learning based CNN. We confirmed the shape of the scaffold that is widely used in cell culture. We produced scaffold specimens, which are widely used in biosensor fabrications. Then, the scaffold specimens were photographed to collect image data necessary for model manufacturing. We produced the detecting model of scaffold warpage defect using Densenet among CNN models. We evaluated the accuracy of the defect detection model with mAP, which evaluates the detection accuracy of deep learning. As a result of model evaluating, it was confirmed that the defect detection accuracy of the scaffold was more than 95%.

• 한국미생물·생명공학회지
• /
• 제45권2호
• /
• pp.93-100
• /
• 2017

인간노로바이러스는 바이러스성 식중독 원인의 대부분을 차지한다. 노로바이러스가 건강한 성인에 감염하면 설사 등의 병변을 몇 일간 일으키다 대부분 별다른 처치 없이도 치유되는 경우가 대부분이다. 그러나 면역기능이 약화된 환자에게 감염한 경우, 만성감염 내지 치명적 감염도 가능한 것으로 보고 되고 있다. 1968년에 처음 노로바이러스가 보고된 이후 세포 감염모델과 소동물감염 모델을 만들고자 하는 시도가 이어져 왔으나 대부분 실패하였다. 그러나 최근들어 세포감염 모델 개발에 있어 주목할 만한 기념비적인 연구들이 이루어졌다 것이 고무적이다 할 수 있다. 이번 총설에서는 새로 개발된 감염 모델들의 특징과 장단점을 살펴보고, 이를 더욱 개선할 수 있는 방향에 대하여 살펴보고자 한다.

• Biomaterials and Biomechanics in Bioengineering
• /
• 제3권3호
• /
• pp.141-155
• /
• 2016

Two-dimensional (2D) cell culture and in vivo cancer model systems have been used to understand cancer biology and develop drug delivery systems for cancer therapy. Although cell culture and in vivo model studies have provided critical contribution about disease mechanism, these models present important problems. 2D tissue culture models lack of three dimensional (3D) structure, while animal models are expensive, time consuming, and inadequate to reflect human tumor biology. Up to the present, scaffolds and 3D matrices have been used for many different clinical applications in regenerative medicine such as heart valves, corneal implants and artificial cartilage. While tissue engineering has focused on clinical applications in regenerative medicine, scaffolds can be used in in vitro tumor models to better understand tumor relapse and metastasis. Because 3D in vitro models can partially mimic the tumor microenvironment as follows. This review focuses on different scaffold production techniques and polymer types for tumor model applications in cancer tissue engineering and reports recent studies about in vitro 3D polymeric tumor models including breast, ewing sarcoma, pancreas, oral, prostate and brain cancers.

• Biotechnology and Bioprocess Engineering:BBE
• /
• 제10권3호
• /
• pp.192-197
• /
• 2005

The effects of macronutrients $(NO_3^-,\; NH_4^+\;and\;PO_4^{3-})$ on cell growth and triterpenoids production in Centella asiatica cell suspension cultures were analyzed using the Box­Behnken response surface model experimental design. In screening and optimization experiments, $PO_4^{3-}$ as a single factor significantly influenced cell growth where increasing the phosphate level from 0.1 to 2.4 or 2.6 mM, elevated cell growth from 3.9 to $14\~16g/L$. The optimum values predicted from the response surface model are 5.05mM $NH_4^+$, 15.0mM $NO_3^-$ and 2.6mM $PO_4^{3-}$, yielding 16.0g/L cell dry weight with $99\%$ fitness to the experimental data. While the $NH_4^+-NO_3^-$ interaction influenced cell growth positively in the optimization experiment, $NH_4^+$ and $NO_3^-$ as single factors; and interactions of $NO_3^--PO_4^{3-},\;NH_4^+-PO_4^{3-}$ and $NH_4^+-NO_3^-$ were all negative in the screening experiment. Cell growth and the final pH level were positively affected by $PO_4^{3-}$, but negatively affected by $NH_4^+\;and\;NH_4^+-PO_4^{3-}$ interactions. The different effects of factors and their interactions on cell growth and final pH are influenced by a broad or narrow range of macronutrient concentrations. The productions of triterpenoids however were lower than 4mg/g cell dry weight.

• 한국미생물·생명공학회지
• /
• 제17권3호
• /
• pp.173-177
• /
• 1989

HeLa 세포주의 연속 배양시 세포수가 배지의 이동속도가 증가함에 따라 감소하는 현상을 나타냈으며, 최대 세포수를 유지할 때의 dilution rate은 0.012(1/h)로 wash-out인 0.050(1/h)보다 극히 낮으며, dilution rate이 0.030(1/h)일 때 2.0(mL of cells/L/h)의 최대 세포 생산속도를 보였다. 또한 낮은 배지 이동속도에서 세포수의 감소에 따른 maintenance term의 존재를 확인했다. 더불어 packed cell volume파 산소소비속도의 측정값이 실제 세포증식과 밀접한 관계가 있음이 입증되어 간접방법에 의한 생육도치 측정이 가능하게 되었다. 또한 산소 yield model에 의해 최대 산소 수율, $Y_{O2}^{max}$과 maintenance 산소소비속도, m$_{O2}$가 각각 4.1$\times$$10^5$(cells/mmole $O_2$)와 10.71$\times$$10^{-9}$(mmole $O_2$/ cells/h)로 측정되었다.