• Title/Summary/Keyword: cell compatibility

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Ultrastructural Study on the Cellular Compatibility in Self-Parasiting Cuscuta australis (자기기생하는 실새삼(Cuscuta australis)에서 세포 화합성에 관한 미세구조 연구)

  • 이규배
    • Journal of Plant Biology
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    • v.36 no.3
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    • pp.285-292
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    • 1993
  • Cellular compatibility in the self-parasitism of Cuscuta australis R. Brown was studied at the ultrastructural level. The front cells of the haustorium penetrated the host stems independently grew within the host tissues and transformed into elongate, filamentous hyphae. Each hyphal cells contained a large nucleus and dense cytoplasm with abundant cell organelles. Multilamellar structures were contained in the cytoplasm and cell walls of the penetrating hyphal cells. When the hyphal cells did not yet invade the host cells, the middle lamella and the fused cellulosic cell walls of the two partners at the host-parasite interface were preserved well. As the invasion of the parasitic hyphal cells progressed, however, the middle lamella was not found at the interface and the host cell walls and plasma membranes were partially broken down. A hyphal cell penetrated deeply into the host cell had a more darkly stained cytoplasm with numerous of cell organelles. In the host cells attacked by the hyphal cells the limiting membranes of plastids were broken down and several vesicles were arrayed near the cell walls. No plasmodesmatal connections between the host and parasite cell walls were found; however, half-plasmodesmata were observed frequently on the side of the hyphal cell walls. These results suggested that the compatibility response in the self-parasitism of Cuscuta was expressed by cell walls, not by plasmodesmata, between the host and the parasite cells.

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Study of statistical distribution for four-port TEM cell

  • Jeon, Sangbong;Kwon, Jong-Hwa
    • Journal of Multimedia Information System
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    • v.1 no.2
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    • pp.127-132
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    • 2014
  • The transverse electromagnetic (TEM) cells are widely used for electromagnetic compatibility (EMC) testing and field probe calibrations. We propose the verification of TEM mode with statistical method using a four-port TEM cell. The verification results are compared with Normal, Rayleigh, and Gamma distribution. As a result, the 75 % quantile of the Rayleigh distribution is excellent agreement with the true quantiles for a number of calibration points.

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Polymer Surfaces for Cell Adhesion II. Cell Culture on Surface-modified Polymers (세포적합성 고분자 표면에 관한 연구 II. 표면 개질된 고분자에의 세포 배양)

  • 이진호;강길선
    • Journal of Biomedical Engineering Research
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    • v.10 no.2
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    • pp.195-202
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    • 1989
  • Chinese Hamster Ovary( CHO) cells were cultured on the surface-modified polymers described in the previous study( "Polymer Surfaces for Cell Adhesion. 1. Surface Modification of Polymers and ESCA Analysis, " J. of KOSOMBE, Vol. 10, No. 1, 43-51, 1989). Among the physicochemical treatment methods. the chloric acid treatment was found to be the best method of rendering the polymer surfaces adhesive for CHO cells probably due to the high density of hydroxyl groups on the surface. Among the biological methods, the fibronectin treatment was best for CHO cell-compatibility probably due to specific active sites existed on the tell-binding domains of the fibronectin structure. When we compare the cell-compatibility of the chloric acid - and the fibronectin -treated PET surfaces, the number of cells attached on the surfaces were increased by 460.5 % and 559.0 % and, respectively, after 32 hr CHO cell culture, compared to that of untreated PET.eated PET.

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Blood Compatibility of Artificial Blood-Contacting Surface Seeded with Cultured Bovine Endothelial Cells (소폐동맥 내피세포를 이용한 인조혈액접촉표면의 혈액 접합성)

  • 김원곤;곽영태;유세영
    • Journal of Chest Surgery
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    • v.26 no.2
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    • pp.80-85
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    • 1993
  • Synthetic and biosynthetic vascular grafts of small diameter have long been considered to be prone to thrombosis, ultimately leading to the complete graft occlusion. Endothelial cell seeding onto synthetic blood-contacting surfaces has been suggested to be an ideal means to solve this problem. This study described a culture method of bovine endothelial cells and evaluated blood-compatibility and seeding efficiency of cultured endothelial cells. Bovine pulmonary artery endothelial cells were harvested enzymatically and grown to confluence on polystyrene culture flask surfaces using established techniques. The identification of endothelial cells was made through the demonstration of expression of factor VIII R:Ag by immunofluorescent technique. To quantitate the effect of improvement in blood-compatibility of viable endothelial cells, endothelial monolayers were exposed to blood containing $\^$111/In-oxine labeled platelets. Viable endothelial monolayers retained less labeled platelets than control surfaces. The Indium-labeled endothelial cells were seeded onto three different blood-contacting surfaces of Dacron vascular graft immobilized in specially equipped wells and incubated for specific time intervals (t=15, 30, 60, 120 minutes). Longer incubation times showed improved cell adherence in collagen-coated and fibrin-coated Dacron vascular graft groups. However in untreated Dacron grafts, no direct relationship was observed between incubation time and endothelial cell seeding efficiency. This may be due to leakage of endothelial cells through porosity of Dacron grafts in this in-vitro experimental condition.

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Study on Compatibility Analysis between Radio Services (무선통신 업무간 양립성 분석에 관한 연구)

  • Kang, Jeong-Yong;Kim, Jin-Young;Kim, Eun-Cheol;Kim, Jong-Heon;Ryu, Chung-Sang;Oh, Seong-Taek
    • The Journal of The Korea Institute of Intelligent Transport Systems
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    • v.6 no.3
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    • pp.122-137
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    • 2007
  • In this paper, compatibility between different radio services or systems is analyzed when they are in adjacent band or co-channel. The first step of the compatibility analysis is establishing an interference scenario. And an interference pobability from an interfering transmitter to a victim receiver is calculated by means of Monte-Carlo simulation techniques. Then the calculated result is compared with the predefined interference criteria, maximum permissible interference probability and we can determine compatibility in accordance with the active interferer density, channel bandwidth, cell radius, distance between interfering transmitter and victim receiver, and duty cycle. It is assumed that Propagation modes are the free space model and extened Hata model.

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Mechanical Property and Cell Compatibility of Silk/PLGA Hybrid Scaffold; In Vitro Study (실크/PLGA 하이브리드 지지체의 기계적 물성과 세포친화력; in vitro 연구)

  • Song, Yi-Seul;Yoo, Han-Na;Eum, Shin;Kim, On-You;Yoo, Suk-Chul;Kim, Hyung-Eun;Lee, Dong-Won;Khang, Gil-Son
    • Polymer(Korea)
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    • v.35 no.3
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    • pp.189-195
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    • 2011
  • The design of new bioactive scaffolds offering physiologic environment for tissue formation is an important frontier in biomaterials research. In this study, we performed compressive strength, water-uptake ability, and SEM analysis for physical property assessment of 3-D silk/PLGA scaffold, and investigated the adhesion, proliferation, phenotype maintenance, and inflammatory responses of RAW 264.7 and NIH/3T3 for cell compatibility. Scaffolds were prepared by the solvent casting/salt leaching method and their compressive strength and water-uptake ability were excellent at 20 wt% silk content. Result of cell compatibility assay showed that inflammatory responses distinctly decreased, and initial adhesion and proliferation were maximized at 20 wt% silk content. In conclusion, we suggest that silk/PLGA scaffolds may be useful to tissue engineering applications.

Cell-compatibility of physicochemically and biologically modified polymer surfaces (물리화학적 및 생물학적으로 표면개질된 고분자의 세포 적합성 연구)

  • Lee, J.H.;Park, K.H.;Khang, G.S.;Lee, H.B.;Andrade, J.D.
    • Proceedings of the KOSOMBE Conference
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    • v.1989 no.05
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    • pp.1-3
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    • 1989
  • We have treated polymer surfaces such as polyethylene, polystyrene and polyester by various physicochemical and biological surface modification methods to be suitable for cell adhesion. The physicochemical methods we used were $O_2$ plasma discharge, corona discharge, sulfuric acid and chloric acid treatments. For the biological treatments, blood proteins such as plasma protein, serum protein and fibronectin were adsorbed onto the polymer surfaces. Chinese Hamster Ovary (CHO) cells were cultured on the surface-modified polymers and the cell-compatibility of those surfaces were compared. The chloric acid and fibronectin treatments were found to be the best methods of rendering the polymer surfaces adhesive for CHO cells.

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Interaction of Fibroblast Cells onto Chloric Acid-treated Poly($\alpha$-hydroxy acid) Polymer Surfaces (염소산 처리된 Poly($\alpha$-hydroxy acid)계 고분자 표면과 섬유아세포의 상호작용)

  • 이상진;강길선;이진호;이영무;이해방
    • Polymer(Korea)
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    • v.24 no.6
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    • pp.877-885
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    • 2000
  • PLA, PGA and PLGA films were treated with chloric acid mixture solution [70% perchloric acid (HClO$_4$)/potassium chlorate (KClO$_3$) aq. saturated solution, 3 : 2] to increase surface wettability and thus cell compatibility. The surface-treated PLA, PGA, and PLGA films were characterized by the measurement of water contact angle, electron spectroscopy for chemical analysis, and scanning electron microscopy. Surface wettability of chloric acid-treated PLA, PGA, and PLGA film surfaces was gradually increased with increase of treatment time. Unlike EtOH pre-treatment, chloric acid-treated polymer films maintain hydrophilic surface after drying. In cell adhesion test, fibroblasts were cultured on the chloric acid-treated film surfaces for 1 and 2 days. As the surface wettability increased, the cell adhesion on the surface were increased. In conclusion, this study demonstrated that the surface wettability of polymer plays an important role for cell adhesion and proliferation behavior.

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A Study on Compatibility between LTE and WLAN System for Mobile Satellite Wireless Package System

  • Ko, Hojeong;Jung, Soonho;Lee, Ilkyoo;Cho, Juphil;Cha, Jae Sang
    • International Journal of Internet, Broadcasting and Communication
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    • v.7 no.2
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    • pp.130-136
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    • 2015
  • In this paper, we analyzed the adjacent channel interference of the LTE band 40 defined by 3GPP on the WLAN system based on the Monte-Carlo method, and found the guard band required for compatibility between the two systems. This study have a difference compared to the previous studies about cell radius was determined using Extended Hata Model considering practical environment, interference protection distance related to guard band, and the spectrum emission mask improvement effect for minimizing the interference. Simulation results, for no channel interference compatibility of LTE and WLAN for mobile satellite wireless package system, we can find the need 15MHz guard band at specified spectrum emission mask and 10MHz guard band at assumed spectrum emission mask.