• BMB Reports
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• v.29 no.4
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• pp.321-326
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• 1996

Spinach glycolate oxidase was subjected to a series of chemical modifications aimed at identifying amino acid residues essential for catalytic activity. The oxidase was reversibly inactivated by treatment with pyridoxal 5'-phosphate (PLP). The inactivation by PLP was accompanied by the appearance of an absorption peak of around 430 nm, which was shifted to 325 nm upon reduction with $NaBH_4$. After reduction, the PLP-treated oxidase showed a fluorescence spectrum with a maximum of around 395 nm by exciting at 325 nm. The substrate-competitive inhibitors oxalate and oxaloacetate provided protection against inactivation of the oxidase by PLP. These results suggest that PLP inactivates the enzyme by fonning a Schiff base with lysyl residue(s) at an active site of the oxidase. The enzyme was also inactivated by tryptophan-specific reagent N-bromosuccinimide (NBS). However, competitive inhibitors oxalate and oxaloacetate could not protect the oxidase significantly against inactivation of the enzyme by NBS. The results implicate that the inactivation of the oxidase by NBS is not directly related to modification of the tryptophanyl residue at an active site of the enzyme. Treatments of the oxidase with cysteine-specific reagents iodoacetate, silver nitrate, and 5,5'-dithiobis-2-nitrobenzoic acid did not affect significantly the activity of the enzyme.

• International Journal of Automotive Technology
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• v.2 no.2
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• pp.63-75
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• 2001

This paper describes the development of THC reduction technologies compliant with SULEV regulations. Technologies embodied by the developmental work include improvement of fuel spray atomization, quick warm-up through coolant control shut of, and acceleration of fuel atomization for the fast rise of cylinder head temp inside the water jacket as well as the improvement of combustion state. The technologies likewise entail reduced HC while operating in lean A/F condition during engine warm-up with the cold lean burn technology, individual cylinder A/F control for improvement of catalytic converting efficiency, after-treatment such as thin-wall catalyst, HC-adsorber and EHC and etc, through vehicle application evaluation in cold start. We carried out an experimental as well as a practical study against SULEV regulations, and the feasibility of adopting these items in vehicle was likewise investigated.

• Transactions of the Korean Society of Automotive Engineers
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• v.16 no.6
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• pp.19-25
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• 2008

Selective Catalytic Reduction is effective in the reduction of NOx emission. This research focused to evaluate the performance of a urea-SCR system and was conducted in two procedures. One is SCR reactor test using model gas in order to provide an optimal injection condition itself. In this step, some parametric study on emission temperature, space velocity, aspect ratio and the formation of urea spray were made by using flow visualization and Computation Fluid Dynamics techniques. The basic simulation results contributed in determining the layout for an actual engine test. The other is an engine performance and emission test. The urea injector was placed at the opposite direction of exhaust gases emitted into an exhaust duct and an optimal amount of a reducing agent is estimated accurately under different engine loads and speeds. Furthermore, the variation of NOx emission and applied amount of urea was investigated in terms of modes under the condition of with and without SCR, and other emissions such as PM, CO and NMHC were evaluated quantitatively as well. This research may provide fundamental data for the practical use of urea-SCR in future.

• Bulletin of the Korean Chemical Society
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• v.35 no.1
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• pp.141-146
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• 2014

A series of bimetallic Cu-Zn/$SiO_2$ catalysts were prepared via thermal decomposition of the as-synthesized $CuZn(OH)_4(H_2SiO_3)_2{\cdot}nH_2O$ hydroxides precursors. This highly dispersed Cu-solid base catalyst is extremely effective for hydrogenation of ethyl acetate to ethanol. The reduction and oxidation features of the precursors prepared by coprecipitation method and catalysts were extensively investigated by TGA, XRD, TPR and $N_2$-adsorption techniques. Catalytic activity by ethyl acetate hydrogenation of reaction temperatures between 120 and $300^{\circ}C$, different catalyst calcination and reduction temperatures, different Cu/Zn loadings have been examined extensively. The relation between the performance for hydrogenation of ethyl acetate and the structure of the Cu-solid base catalysts with Zn loading were discussed. The detected conversion of ethyl acetate reached 81.6% with a 93.8% selectivity of ethanol. This investigation of the Cu-Zn/$SiO_2$ catalyst provides a recently proposed pathway for ethyl acetate hydrogenation reaction to produce ethanol over Cu-solid base catalysts.

• Bulletin of the Korean Chemical Society
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• v.29 no.7
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• pp.1386-1390
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• 2008

Artemisinin, the effective ingredient of Chinese herb Artemisia annua L (Qinghao in Chinese), has been proved to be effective to antimalarial. Herein, a reliable, sensitive and convenient electrochemical method was developed for the determination of artemisinin utilizing the excellent properties of multi-wall carbon nanotube (MWNT). The electrochemical behavior of artemisinin was investigated. It is found that the reduction peak current of artemisinin remarkably increases and the peak potential shifts positively by 240 mV at the MWNT film-modified electrode. These phenomena indicate that the MWNT film exhibits efficient catalytic activity to the electrochemical reduction of artemisinin. The effects of pH value, amount of MWNT, scan rate and accumulation time were examined. The limit of detection (S/N = 3) is as low as 10 $\mu$ g $L^{-1}$. Finally, this newly developed method was used to determine the content of artemisinin in Artemisia annua L.

• Bulletin of the Korean Chemical Society
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• v.33 no.11
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• pp.3581-3588
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• 2012

A new series of acyclic mononuclear gadolinium(III) complexes have been prepared by Schiff-base condensation derived from 5-methylsalicylaldehyde, diethylenetriamine, tris(2-aminoethyl) amine, triethylenetetramine, N,N-bis(3-aminopropyl)ethylene diamine, N,N-bis(aminopropyl) piperazine, and gadolinium nitrate. All the complexes were characterized by elemental and spectral analyses. Electronic spectra of the complexes show azomethine (CH=N) within the range of 410-420 nm. The fluorescence efficiency of Gd(III) ion in the cavity was completely quenched by the higher chain length ligands. Electrochemical studies of the complexes show irreversible one electron reduction process around -2.15 to -1.60 V The reduction potential of gadolinium(III) complexes shifts towards anodic directions respectively upon increasing the chain length. The catalytic activity of the gadolinium(III) complexes on the hydrolysis of 4-nitrophenylphosphate was determined. All gadolinium(III) complexes were screened for antibacterial activity.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.11 no.6
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• pp.28-35
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• 2012

This study is on SCR reactor, NOx reduction system in Marine that has been an issue nowadays. Especially design data was obtained by numerical on flow uniformity that is one of the design factor in SCR reactor. Also pressure drop on catalyst size inserted into SCR reactor was compared by experiment and numerical analysis. S/W, numerical analysis used for this study was confirmed that the result of numerical analysis used STAR-CCM+, common use CFD code, pressure drop on catalyst is not big different from the result of numerical analysis. In addition, degree of uniformity of liquid on SCR reactor was over 0.9. Whereas it was assured that degree of uniformity of liquid was changed depends on the shape of pipe at the entrance of SCR.

• Journal of the Korean Chemical Society
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• v.37 no.8
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• pp.744-752
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• 1993

Electrochemical reduction of thionyl chloride has been carried out at glassy carbon and microelectrode that modified by macrocyclic compounds. The catalyst molecules of macrocyclic compounds were adsorbed on the electrode surface and reduced thionyl chloride resulting in a generation of oxidized catalyst molecules. The concentration of catalysts and electrode immersion time were found to affect the catalyst performance strongly. Significant improvements in cell performance have been noted in terms of both exchange rate constants of up to 10 times and power densities of up to 220% at glassy carbon electrode. The diffusion coefficients obtained at carbon microelectrode were slightly different from that determined at glassy carbon electrode.

• Journal of Electrochemical Science and Technology
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• v.10 no.3
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• pp.313-320
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• 2019

In this study, cobalt diselenide ($CoSe_2$) and the composites ($CoSe_2@RGO$) of $CoSe_2$ and reduced graphene oxide (RGO) were synthesized by a facile hydrothermal reaction using cobalt ions and selenide source with or without graphene oxide (GO). The formation of $CoSe_2@RGO$ composites was identified by analysis with X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and scanning electron microscopy (SEM). Electrochemical analyses demonstrated that the $CoSe_2@RGO$ composites have excellent catalytic activity for the reduction of $I_3{^-}$, possibly indicating a synergetic effect of $CoSe_2$ and RGO. As a consequence, the $CoSe_2@RGO$ composites were applied as a counter electrode in DSSC for the reduction of redox couple electrolyte, and exhibiting the comparable power conversion efficiency (7.01%) to the rare metal platinum (Pt) based photovoltaic device (6.77%).

• Journal of Institute of Convergence Technology
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• v.8 no.1
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• pp.5-8
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• 2018

Emissions of diesel vehicles have been regulated by NEDC mode for a long time. However, the NEDC mode has been known the control of emission reduction is not reflected properly on actual road conditions. For these reasons, diesel vehicle emissions are regulated in both NEDC mode and WLTC mode from 2017 to 2020, from 2020 onwards, the emissions of diesel vehicles will measure in WLTC mode only and will not be able to exceed 1.5 times the regulated value. The purpose of this study is to analyze the development trend of diesel vehicle after-treatment system in order to comply with the future regulations on diesel vehicle. As a result, it is essential to reduce the NOx emissions of diesel vehicles for Euro 6, the NOx emissions of the test vehicle equipped with SCR were 30% to 50% loss than the test vehicle equipped with LNT despite the higher curb weight and engine displacement.