• 한국수정란이식학회지
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• 제31권2호
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• pp.109-116
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• 2016

The recovery of epididymal sperm in animals is considered as one of the important tools to preserve high value or endangered species. However, there are no appropriate castrating indicators such as months of age in bull, sperm morphology, and motility, particularly in young Korean native bull (Hanwoo). Therefore, this study aimed to investigate sperm number, morphology, and motility of sperm in the epididymis tail of young Hanwoo bulls at 8 and 15 months of age. After castration, epididymal tails were collected and minced with blades to recover sperm. In experiments 1 and 2, sperm number, morphology, and motility were examined. Total number of sperm and percentage of normal sperm from bulls at 8 months of age was lower than that of bulls at 15 months of age after collection (P<0.05). Percentage of abnormal head, tail, proximal cytoplasmic droplet, dead and damaged acrosome of sperm from bulls at 8 months of age were higher than those of bulls at 15 months of age (P<0.05). In experiment 3, sperm motility from bulls at 8 and 15 months of age were examined before freezing and after thawing. Frozen-thawed sperm at 8 months of age showed low total motility and motile sperm with ${\geq}25{\mu}m/sec$ compared to those at 15 months of age and commercially-used sperm (P<0.05). In conclusion, sperm derived from the epididymal tail of bulls at 8 months of age showed high abnormal morphology and poor motility, which are not adequate for AI and IVF. On the other hand, sperm derived from the epididymal tail of bulls at 15 months of age showed high normal morphology and motility.

• 한국축산식품학회지
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• 제31권2호
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• pp.257-265
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• 2011

본 연구는 한우의 거세유무 및 도축월령에 따른 비육능력 및 도체의 이화학적 특성 비교를 구명하기 위하여 생후 13개월령의 비육한우 36두(비거세한우 18두 - 3처리 ${\times}$6두; 거세한우 18두 - 3처리 ${\times}$두)를 공시하여 생후 26개월, 28개월, 및 30개월령까지 비육한 후, 도체특성, 도체의 이화학적 특성 및 지방산 조성을 조사하였다. 도체의 냉도체중은 도축월령이 길어짐에 따라 비거세우와 거세우 모두 증가하였다(p<0.05). 등지방 두께에서 비거세우군은 28개월령이, 거세우군은 30개월령이 가장 두껍게 나타났고(p<0.05), 배최장근 단면적에서 비거세우군과 거세우군 모두 28개월령 때가 가장 넓게 나타났다(p<0.05). 근내지방도, 가열감량 및 보수력은 양 처리군 모두 30 개월령 도축할 때 가장 높았고(p<0.05), 비육기간이 길어질수록 높아지는 경향이었다. 전단력의 경우 비거세우군은 30개월령일 때에, 거세우군에서는 28개월령에 높았다. 도체의 불포화 지방산(unsaturated fatty acid, UFA)은 양 처리군에서 28개월령과 30개월령이 유의적으로 높았다(p<0.05). 이상의 연구결과를 종합해 볼 때, 한우의 장기비육에 있어서 늦게라도 거세를 실시하면 뚜렷한 도체등급의 개선 효과가 있으며, 거세비육우의 도축월령은 육질적측면(마블링 스코어와 보수력)에서 생후 28개월과 30개월령 간에는 거의 차이가 없었다.

• 대한수의학회지
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• 제6권1호
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• pp.18-23
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• 1966

The protein-bound iodine-131, the concentration of iodine-131 in blood, and the excretion rate of I-131 through urine and feces were observed in nine Korean native goats, 3 months age, following administration of $3{\mu}C$ of I-131 per kg of body weight. No signiant differences were found due to sex and castration. 1. The average protein-bound iodine-131 conversion ratio of goats was 16.7% in 24 hours. In castrated group, the lowest proteinbound iodine-131 conversion ratio was observed. 2. The average concentration of iodine-131 in bleed, increased very rapidly by 2 hours(4.75%) and rapidly decreased within 6 hours(0.73%). 3. The average excretion rate of I-131 through urine was highest in 24 hours(19.00%) and decreased rapidly within 48 hours(5.32%). 4. The average excretion ration rate of I-131 though feces was highest in 24 hours(2.55%), and decreased slowly.

• Korean Journal of Radiology
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• 제25권2호
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• pp.179-188
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• 2024

Objective: ¹⁷⁷Lutetium [Lu] Ludotadipep is a novel prostate-specific membrane antigen targeting therapeutic agent with an albumin motif added to increase uptake in the tumors. We assessed the biodistribution and dosimetry of [¹⁷⁷Lu]Ludotadipep in patients with metastatic castration-resistant prostate cancer (mCRPC). Materials and Methods: Data from 25 patients (median age, 73 years; range, 60-90) with mCRPC from a phase I study with activity escalation design of single administration of [¹⁷⁷Lu]Ludotadipep (1.85, 2.78, 3.70, 4.63, and 5.55 GBq) were assessed. Activity in the salivary glands, lungs, liver, kidneys, and spleen was estimated from whole-body scan and abdominal SPECT/CT images acquired at 2, 24, 48, 72, and 168 h after administration of [¹⁷⁷Lu]Ludotadipep. Red marrow activity was calculated from blood samples obtained at 3, 10, 30, 60, and 180 min, and at 24, 48, and 72 h after administration. Organand tumor-based absorbed dose calculations were performed using IDAC-Dose 2.1. Results: Absorbed dose coefficient (mean ± standard deviation) of normal organs was 1.17 ± 0.81 Gy/GBq for salivary glands, 0.05 ± 0.02 Gy/GBq for lungs, 0.14 ± 0.06 Gy/GBq for liver, 0.77 ± 0.28 Gy/GBq for kidneys, 0.12 ± 0.06 Gy/GBq for spleen, and 0.07 ± 0.02 Gy/GBq for red marrow. The absorbed dose coefficient of the tumors was 10.43 ± 7.77 Gy/GBq. Conclusion: [¹⁷⁷Lu]Ludotadipep is expected to be safe at the dose of 3.7 GBq times 6 cycles planned for a phase II clinical trial with kidneys and bone marrow being the critical organs, and shows a high tumor absorbed dose.

• Asian Pacific Journal of Cancer Prevention
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• 제16권15호
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• pp.6615-6619
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• 2015

Background: The expression of HER-2 in prostate cancer has been linked to disease progression. We analysed the presence of HER-2 expression in primary tumors in men undergoing radical prostatectomy, its association with clinical and pathological findings, and its expression in secondary circulating prostate cells (CPCs) during follow up, as well as links with biochemical failure and the effects of androgen blockade. Materials and Methods: Consecutive men undergoing radical prostatectomy for histologically confirmed prostate cancer were analyzed. HER-2 expression in the primary tumor was assessed using the HercepTest(R), CPCs were identified from blood samples using standard immunocytochemistry with anti-PSA and positive samples with the HercepTest(R) to determine HER-2 expression. The influence of HER-2 expression on the frequency of biochemical failure and effects of androgen blockade was determined. Results: 144 men with a mean age of $64.8{\pm}10.3$ years participated, with a median follow up of 8.2 years. HER-2 was expressed in 20.8% of primary tumors; it was associated with vascular infiltration and older age, but not with other clinical pathological findings. Some 40.3% of men had secondary CPCs detected, of which 38% expressed HER-2. Men CPC (+) had a higher frequency of biochemical failure, but there was no difference in HER-2 expression of CPCs with the frequency of biochemical failure. After androgen blockade, men with HER-2 (+) positive secondary CPCs had a higher frequency of disease progression to castrate resistant disease. Conclusions: HER-2 plays a dual role in the progression of prostate cancer; firstly it may increase the potential of tumor cells to disseminate from the primary tumor via the blood by increasing vascular infiltration. In the presence of androgens, there is no survival advantage of expressing HER-2, but once biochemical failure has occurred and androgen blockade started, HER-2 positive cells are resistant to treatment, survive and grow leading to castration resistant disease.

• Journal of Animal Science and Technology
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• 제52권5호
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• pp.427-434
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• 2010

본 연구는 거세, 월령별 적정 사료 급여관리 및 아미노산 강화 반추위 보호지방산(ruminally protected amino acid-enriched fatty acid, RPAAFA)의 급여가 당대검정 탈락축의 산육능력 및 육질 향상에 미치는 영향을 조사하기 위해서 수행되었다. 당대검정 탈락축은 생후 14개월령에 거세를 실시하였다. 시험구 배치는 거세 한우 16두(15개월령, 평균 체중 $412.9{\pm}24.9kg$)를 12개월 동안 RPAAFA를 급여하지 않는 대조구(control) 및 100g 급여하는 처리구(treatment)의 2처리로 완전임의 배치하였다. 거세한우는 생후 27월령에 도축하였다. 대조구에 비해 처리구의 일당증체량이 높은 경향을 보였으며, 사료요구율은 낮은 경향이었다. RPAAFA의 급여가 배최장근단면적, 등지방두께, 육색, 지방색, 조직감 및 성숙도에 미치는 영향은 없었다. 육량 A등급 및 육질 l등급 이상의 고급육 출현률은 대조구에 비해 처리구에서 높은 경향이었다. 배최장근의 수분, 지방, 단백질 및 회분 함량은 처리간 유사한 수준이었다. RPAAFA의 급여가 배최장근의 보수력, 산화환원전위, 총육색소 및 지방산 함량에 미치는 영향은 없었다. 따라서 거세, 적정 사료급여 관리 및 RPAAFA의 급여는 당대검정 탈락축의 산육능력 및 육질등급 향상에 긍정적인 영향을 미치는 것으로 사료된다.

• 대한비뇨기종양학회지
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• 제16권3호
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• pp.126-134
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• 2018

Purpose: The purpose of this study is to compare the radiation therapy (RT) and radical prostatectomy (RP) of high-risk or locally advanced prostate cancer (PC) patients after neoadjuvant hormonal therapy (NHT). Materials and Methods: This retrospective study evaluated patients underwent RT (42 patients) or RP (152 patients) after NHT at a single center during 2003-2014. Times to biochemical recurrence (BCR), pelvic local recurrence (PLR), metastasis, clinical painful symptom progression (CPSP), castration-resistant PC (CRPC), and overall survival were compared between the RT and RP groups, after adjustment for TN stage, using the Kaplan-Meier method and log-rank test. Results: Significant inter-group differences were observed for age, Gleason score, initial PSA, and clinical and pathological T stages (all p<0.05). During a median follow-up of 71.7 months, the overall incidences of BCR, PLR, metastasis, CPSP, CRPC, and death were 49.5%, 16.5%, 8.3%, 7.7%, 7.7%, and 17.5%, respectively. The median times to BCR were 100 months for RT and 36.2 months for RP (p=0.004), although the median times were not reached for the other outcomes (all p>0.05). The independent predictor of CPSP was RP (hazard ratio, 0.291; p=0.013). Conclusions: Despite significantly different baseline parameters, RP provided better CPSP-free survival than RT among patients with localized high-risk or locally advanced PC.

• 한국임상수의학회지
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• 제22권4호
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• pp.357-364
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• 2005

We surveyed the incidence of urinary calculi for feeding period, urinary lesion, composition of calculi and changes of serum chemistry with Korean native castrated steers, which are growing until 26 through 31 months. Without showing any clinical signs such as urethral obstruction, hydronephrosis and strangury for all group, $37.8\%$ (34 out of 90) of Korean native steers carried urinary calculi in carcass. The incidence of urine calculi far different feeding stages from 26 to 31 months were observed as $13.3-60.0\%$er, no significant differences were detected. The distribution of calculi were kidney $(24.4\%)$, kidney and bladder $(10\%)$ and kidney, bladder and urine $(3.3\%)$. Congestion of urinary bladder was shown in $26.6\%$ of Korean native steers. Korean native steers with urinary calculi $(41.2\%)$ had more possibilities (P<0.05) to bring outbreaks of congestion of urinary bladder than those of without calculi $(17.8\%)$. The main composition of calculi were struvite (magnesium ammonium phosphate crystals), calcium phosphate, calcium carbonate, calcium oxalate, non-oxalate calcium, ammonia and phosphate. The distribution of urine pH was $71.7\%$(above pH 8.0), $12.2\%$ (between pH 7.0 and pH 8.0) and $16.6\%$ (under pH 7.0). Like shown in herbivores, most of the urine pH was distributed as alkaline urine. Serum creatinine was shown significant difference (P<0.05) according to incidence of calculi but other serum chemistry didn't show any difference in serum chemistry. These results suggest that feeding until 26 to 31 months in Korean native steers castrated at 6 months of the age elicits urinary calculi and congestion of urinary bladder, but not clinical abnormality. And adjustment of the dietary Can ratio to a level of 2:1 or greater during feeding period may reduce the possibility of incidence of urinary calculi in Korean native steers.

• Asian-Australasian Journal of Animal Sciences
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• 제15권6호
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• pp.838-843
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• 2002

The objectives of this study were to examine growth performance and meat quality by three different feeding patterns in Korean native cattle (KNC). In each of 3 years, fifteen KNC were randomly assigned in a (3 feeding management)${\times}$(3 sex) factorial design experiment; thus, in total, there were 5 animals in each of the 9 treatments. The three feeding management treatments were longterm (24 month) restriction feeding (LTFR), long-term restriction feeding-hormone implant (LTFR-tH), and short-term (18 month) nonrestriction feeding (STFNR). Three sexes were bull, steer, and heifer. Concentrate diet was fed restriction-feeding method based on body weight in LTFR and LTFR-tH. However, the diet was fed ad libitum in STFNR. Hormonal implantation was made three times with M-$PO^{TM}$ for bulls and with F-$TO^{TM}$ for heifers at 18, 20, 22 month of age in LTFR-tH. Animal were purchased from the local cattle market and managed in two local farms and at the university research unit. Animals were slaughtered at 24 months for long-term trial and at 18 month for short-term trial. The growth rate was the highest in bulls and the lowest in heifers. However, the differences were diminished in F-$TO^{TM}$ implanted heifers. The average daily gain was high in STFNR due to ad libitum feeding. The carcass grade was similar among the treatments on percentage bases. Hormonal implants improved significantly the meat quality grade in all sexes. Castration increased body fat content and improved meat quality grade by intramuscular fat deposition. In conclusion, long-term feeding and hormone treatment increased meat quality grade more than short-term feeding. However, ADG was higher in the short-term trial although feedefficiency was lower.

• 한국축산식품학회지
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• 제26권2호
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• pp.183-188
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• 2006

본 연구는 도체 등급 판정을 받은 비육돈 YL 및 YLD 종중 암퇘지 128두와 거세돈 528두, 총 656두를 공시동물로 성별 및 품종별로 도체 등급 출현율을 조사하고, 도체 및 육질특성을 파악하여 고급 돈육 생산에 필요한 기초적 자료를 얻고자 실시하였다. 상위 도체등급(A와 B등급)은 전체 중 62.19%의 높은 출현율을 보였고, 성별로 암퇘지가 67.96%와 거세돈이 60.80%로 암퇘지가 거세돈보다 높았다. 도체중 전체 평균치는 $88.7{\pm}8.71 kg$, 등지방 두께 평균은 $24.3{\pm}4.94 mm$, 육색도 평균은 $3.5{\pm}0.77$, 보수력 평균은 $52.5{\pm}37.94$, 근내 지방도 평균은 $1.7{\pm}0.88$, 평균 도체등급은 $2.8{\pm}1.19$, 그리고 출하 평균 일령은 $176.8{\pm}8.24$일이었다. 성별 도체 특성에서 도체중은 암컷보다 거세돈에서 약간 높은 경향을 보였고, 등지방 두께는 암컷보다 거세돈에서 유의하게(p<0.05) 두꺼웠다. 육색도, 근내 지방도, 보수력 및 출하일령은 암컷과 거세돈에서 유의차는 없었으나, 도체등급은 암컷이 거세돈보다 다소 높은 경향을 보였고, PSE 출현을은 암컷이 4.7%로 거세돈 12.7%보다 우수하였다. 도체중별 도체 특성에서 등지방 두께는 도체중이 무거울수록 유의하게(p<0.05) 두꺼웠다. 육색도와 출하일령은 도체중간에 유의성은 없었으나, 근내지방도와 보수력은 도체중간에 유의한(p<0.05) 차이를 보였다. 등지방 두께별 도체 특성에서 도체증은 등지방 두께간에 유의성 있게(p<0.05) 등지방 두께가 두꺼울수록 도체중도 함께 무거웠다. 근내 지방도와 PSE 출현율은 등지방 두께간에 유의성 있는 차이는 없었다. YL종과 YLD종에서 등지방 두께가 중간층인 $21{\sim}25 mm$군에서 각각 44.8%와 32.0%로 가장 높은 빈도를 보였다. 이상의 결과로 보면 도체등급과 육질이 양호한 돼지고기를 생산하기 위해서는 너무 단축된 출하일령, 지나친 도체중, 너무 얇은 등지방 두께 등이 되지 않도록 적절하게 관리해야 바람직할 것으로 사료되었다.