• Journal of Korean society of Dental Hygiene
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• v.13 no.3
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• pp.509-515
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• 2013

Objectives : This study was performed to evaluate the salivary secretion, salivary pH and cariogenic activity using unstimulated whole saliva in patients with hematologic malignancy. Methods : Nineteen patients (9 male, 10 female) who had hematologic malignancy and were treated with chemotherapy or bone marrow transplantation, and nineteen normal volunteers (7 male, 12 female) as control group were included. The mean age of patients group and control group was 45.1 and 46.7 years, respectively. Patients group was examined salivary secretion, salivary pH, and cariogenic activity using unstimulated whole saliva and was compared with control group. Results : In comparison with control group, salivary secretion, salivary pH and salivary buffer capacity were significantly lower in patients with hematologic malignancy (p<0.01). Both cariogenic activity(p<0.01) and the number of Lactobacilli(p<0.05) are higher in patients group than control group. Conclusions : These results suggest that the unstimulated whole salivary secretion, pH and buffer capacity were lower in patients with hematologic malignancy than control group. Cariogenic activity is higher in patients with hematologic malignancy than control group. Such salivary factor and cariogenic activity can increase the possibility of induction of dental caries.

• International Journal of Oral Biology
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• v.39 no.2
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• pp.57-63
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• 2014

The purpose of this study is to evaluate salivary flow rate, salivary pH, and cariogenic activity using unstimulated saliva of the head and neck cancer patients. Twenty three cancer patients (19 males, 4 females) who had undergone chemotherapy and radiation therapy and twenty four healthy volunteers (14 males, 10 females) as a control were included. Salivary flow rate, salivary pH, and cariogenic activity using unstimulated saliva were examined. Compared to saliva of the control group, salivary flow rate (p<0.001) and salivary pH (p<0.001) were significantly lower in head and neck cancer patients. The colony counts of Lactobacilli was higher in head and neck cancer patients (p<0.05) than in control group. These salivary factors and cariogenic activity can increase the prevalence of dental caries in head and neck cancer patients.

• Journal of Microbiology and Biotechnology
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• v.26 no.11
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• pp.1829-1835
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• 2016

Dental caries is caused by cariogenic biofilm, an oral biofilm including Streptococcus mutans. Recently, the prevention of dental caries using various probiotics has been attempted. Lactococcus lactis HY 449 is a probiotic bacterium. The aim of this study was to investigate the effect of L. lactis HY 449 on cariogenic biofilm and to analyze its inhibitory mechanisms. Cariogenic biofilm was formed in the presence or absence of L. lactis HY 449 and L. lactis ATCC 19435, and analyzed with a confocal laser microscope. The formation of cariogenic biofilm was reduced in cultures spiked with both L. lactis strains, and L. lactis HY 449 exhibited more inhibitory effects than L. lactis ATCC 19435. In order to analyze and to compare the inhibitory mechanisms, the antibacterial activity of the spent culture medium from both L. lactis strains against S. mutans was investigated, and the expression of glucosyltransferases (gtfs) of S. mutans was then analyzed by real-time RT-PCR. In addition, the sucrose fermentation ability of both L. lactis strains was examined. Both L. lactis strains showed antibacterial activity and inhibited the expression of gtfs, a nd t he d ifference b etween both strains did not show. In the case of sucrose-fermenting ability, L. lactis HY 449 fermented sucrose but L. lactis ATCC 19435 did not. L. lactis HY 449 inhibited the uptake of sucrose and the gtfs expression of S. mutans, whereby the development of cariogenic biofilm may be inhibited. In conclusion, L. lactis HY 449 may be a useful probiotic for the prevention of dental caries.

• Natural Product Sciences
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• v.17 no.4
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• pp.267-272
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• 2011

In our search for bioactive phenolics from plants, the culms of Phyllostachys bambusoides has been selected for investigation of anti-cariogenic and 1,1-diphenylpicrylhydrazyl (DPPH) radical scavenging agents based on the initial screening results. Fractionation process of n-hexane and $CHCl_3$ extracts afforded four phenolic constituents, ferulic acid (1), vanillin (2), coniferaldehyde (3), and coniferyl alcohol (4) as guided by their DPPH free radical scavenging activities. Additionally, activity-guided fractionation of EtOAc extract with anti-cariogenic activity has resulted in the isolation of coniferaldehyde (3), 2,6-dimethoxy-p-benzoquinone (5), p-methoxycinnamic acid (6), (${\pm}$)-balanophonin (7), and 6-methoxychromanone (8). The structures of 1 - 8 were determined by spectroscopic data interpretation, and also by comparison of their data with the published values. Phenolic compounds 1 - 4 exhibited similar DPPH radical scavenging activities compared with the synthetic antioxidant, butylated hydroxytoluene (BHT), and compounds 3 and 5 - 8 showed significant antibacterial activity against cariogenic oral streptococci, Streptococcus mutans and S. sobrinus.

• Journal of the korean academy of Pediatric Dentistry
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• v.24 no.3
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• pp.610-627
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• 1997

The purpose of this study was to compare the caries activity and cariogenic factors in children in different residence areas. The subjects of the study were 268 kindergarten children who were selected from the downtown area(D), apartment area(A), suburban area(S), and rural area(R) in and around the city of Iksan, Korea. Caries activity was measured as past caries experience (dft index and dfs index) and salivary reductase activity (Resazurin Disc Test scores). Cariogenic factors were investigated by a questionnaire. The results were as follows: Reductase activity S > R > D > A dt index R, S > D, A ft index D - the highest dft index A - the lowest ds index R - the highest fs index D - the highest Toothbrushing frequency A - the highest, R - the lowest Toothbrushing before bed D, A > S, R Toothbrushing by parents S, R > A, D Sweets intake freqeuncy A - the highest, S - the lowest Distance to dental office D < A < S < R Dental treatment experience D - the highest, A - the lowest Flouride application rate D > S > R > A Sealant application rate D > A, S > R Birth order A, S > R, D Age D > S > R, A

• Journal of Physiology & Pathology in Korean Medicine
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• v.21 no.1
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• pp.70-75
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• 2007

In the present study, we has been isolated the anti-cariogenic component, stigmasterol, from Aralia continentalis (A. continentalis) and identified by MS, $^1$H-NMR and $^{13}$C-NMR and also investigated the anti-cariogenic properties of stigmasterol. The methanol extract of ,A. continentalis showed concentration-dependent inhibitory activity against the growth and acid production of S. mutans. The MeOH extract was suspended in H$_2$O and sequentially partitioned with n-hexane, CHCl$_3$, EtOAc, and n-BuOH. The CHCl$_3$ fraction showed remarkable antibacterial activity against S. mutans. The anti-cariogenic compound, stigmasterol, has been isolated successively through the screening system and various chromatography methods. Anti-cariogenic properties of stigmasterol were also investigated. From this active chloroform subfraction, isolation and identification finally gave (24E)-stigmasta-5,22-dien-3${\beta}$-ol (stigmasterol) {[a]$_D\;^{25}$ -48.33$^{\circ}C$(C 0.28, CHCl$_3$)} by spectroscopic methods (MS, $^1$H-NMR and $^{13}$C-NMR) as an active principle. The compound, stigmasterol, showed significant growth, acid production, adhesion and water-insoluble glucan synthesis inhibitory effect against S. mutans. These results suggest that stigmasterol from ,A. continentalis may inhibit cariogenic properties of S. mutans and these properties may provide some scientific rationales that the local inhabitants used the extracts for treatment of dental diseases.

• Archives of Pharmacal Research
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• v.15 no.3
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• pp.239-241
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• 1992

The methanol extract of Cinnamoni Cortex showed antibacterial action against cariogenic bacterium, Streptococcus mutans OMZ 176. The active principle of the extract was identified to be trans-cinnamaldehyde, which was bactericidal in the minimal inhibitory concentration (MIC) of $100\;\mu$g/ml against the strain. From the results of antibacterial activity of cinnamaldehyde and its derivatives, the acrolein group in the cinnamaldehyde was elucidated to be an essential element for the activity.

• Archives of Pharmacal Research
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• v.13 no.1
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• pp.117-119
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• 1990

A combination of magnolol or honokiol with lysozyme isolated from the egg white of the Korean Ogol fowl (Korean natural monument No.265) exhibited synergistic effect of bactericidal activity against a typical cariogenic bacterium, Streptococcus mutans OMZ 176. The synergistic ratio increased with time dependence.

• Archives of Pharmacal Research
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• v.14 no.1
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• pp.41-43
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• 1991

For the purpose of survey of the antibacterial activity against a cariogenic bacterium Streptococcus mutans OMZ 176 with the introduction of hydroxyl and allyl groups to o-phenylphenol (Fig. 2, 1), 4-hydroxy-o-phenylphenol (2), and 3,6-diallyl-4-hydroxy-o-phenylphenol (4) were synthesized, sucessively. The synthesized compounds, 2 and 4 showed more potent antibacterial activity than the starting material, 1. The hydroxyl group was supposed to the essential element for the antibacterial activity and the introduction of allyl group to phenolic ring to be another element to increase the activity.

• YAKHAK HOEJI
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• v.36 no.1
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• pp.36-39
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• 1992

In a continuous study for the developing of the anticariogenic agents, 3,3'-diacyl-4,4'-biphenol derivatives (Fig. 2, 2 and 3) and 3,3'-bis-(1-hydroxyalkyl)-4,4'-biphenol (Fig. 3, 4 and 5) derivatives are synthesized successively from 4,4'-diphenol (Fig. 2, 1). The synthesized compounds are tested for their antibacterial activity against a cariogenic bacterium, Streptococcus mutans OMZ 176. The acyl derivatives, 2 and 3, do not show antibacterial activity, but the hydroxyalkyl derivatives, 4 and 5, reduced from the acyl group of 2 and 3, show the activity. The antibacterial activity of 2 and 3 may be inhibited due to intramolecular hydrogen bonding between the acyl group and the hydroxyl one (Fig. 4).