• KSBB Journal
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• v.29 no.3
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• pp.155-164
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• 2014

Succinic acid, a representative biomass-derived platform chemical, is a major fermentation product of Actinobacillus succinogenes. It is well known that carbon dioxide is consumed during the succinate fermentation, but the biochemical mechanism behind this phenomenon is not yet understood well. In this study, it was found that the addition of carbonic anhydrase (CA)s into media significantly enhances the succinic acid production by A. succinogenes during the fermentation supplied with carbon dioxide. It is likely that the (bi) carbonate produced by the CA activity from gaseous carbon dioxide is favoured by A. succinogenes for consumption and utilization. Therefore, the $MgCO_3$ requirement could be significantly reduced without compromising the succinate productivity. Furthermore, because of too high price of the commercial carbonic anhydrase, it was undertaken to economically overproduce a cyanobacterial carbonic anhydrase by the use of a recombinant Pichia pastoris. An expression vector system was constructed with the carbonic anhydrase gene PCR-cloned from Cyanobacterium Synechocystis sp., and introduced into P. pastoris for fermentation studies. About 95.9 g/L of succinic acid was produced in the production medium with 30 ppm of carbonic anhydrase, approximately 2 fold higher productivity compared to the parallel process with no supplementation of the enzyme. It is expected that this method can provide a valuable way of overcoming inefficiencies inherent in gas supply during $CO_2$-based bioprocesses like succinic acid fermentation.

• Proceedings of the Korean Nuclear Society Conference
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• 1996.11a
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• pp.127-132
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• 1996

Total organic carbon (TOC) derived from organics is one of tile possible contamination in the reactor water system and causes of pH change and high conductivity levels. Measurements of total dissolved oxidizable carbon were carried out and its contribution to conductivities at elevated temperature was studied by using tile thermodynamic equilibrium analysis in carbonic acid system. The calculated conductivities were in good agreement with tile levels observed for tile formation of carbonic acid from salicylic acid solution.

• KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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• v.25 no.2
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• pp.31-35
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• 2019

Kimchi is a refreshing sour food which gives sour and carbonic acid taste of carbon dioxide produced during the fermentation process. So, carbon dioxide injection was tried to raise carbonic acid taste of kimchi stored in the airtight container. First, carbon dioxide injection times of a given gas supply system were determined experimentally to attain initial concentration of 80% for different solid/liquid ratios. Since carbon dioxide is dissolved in kimchi to decrease its concentration during storage, periodical carbon dioxide injection conditions were needed and determined to keep the $CO_2$ concentration above 70%. For the initial flushing to 80% $CO_2$ concentration in model system filled with water, the injection time ranged from 40 to 89 seconds for free volumes of 2-8 L. $CO_2$ injection conditions for the under-ripened storage at $10^{\circ}C$ consisted of longer time at more frequent cycles for watery kimchi than for Chinese cabbage kimchi. At $0^{\circ}C$ of subsequent ripened stage storage of watery kimchi, the periodical injection at 3 hour interval was required because of continuous dissolution of carbon dioxide. However, Chinese cabbage kimchi did not require subsequent $CO_2$ injection during the ripened state storage and needed only flushing to 80% $CO_2$ at time of the container opening and closing. These results can be used as basic information for the programmed control of $CO_2$ injection in the kimchi container system.

• The Journal of Korean Academy of Prosthodontics
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• v.41 no.5
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• pp.596-605
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• 2003

Statement of problem : IPS Empress2 system was developed and used in prosthodontic treatment, but the cost of ingot is expensive for wide application. Purpose : This study was to investigate the possibility on recycling of IPS Empress 2 ceramic for wide application of IPS Empress 2 ceramic in prosthodontic treatment. Material and Method : 1st, 2nd and 3rd pressed disc-shaped($10{\times}1.5mm$) IPS Empress 2 specimens were made with IPS Empress ingot(200, Ivoclar, Liechtenstein) and pressing furnace(IPS Empress EP 500, Ivoclar, Liechtenstein). Vicker's surface hardness and fracture toughness, acid resistance, and pressing accuracy of IPS Empress 2 ceramic were measured and analyzed. Surface hardness was measured by microhardness tester(MTX 70. Matsuzawa, Japan), before and after surface treatment with 0.5% hydrofluoric acid and carbonic acid(Coca cola) for evaluation of acid resistance. Results : The surface hardness of 1st pressed specimen was the higher(5.11 GPa) than those of 2nd pressed(4.89 GPa) and 3rd pressed specimen(4.86 GPa), and the fracture toughness of 1st pressed ($1.58MPam^{1/2}$) and 2nd pressed specimen($1.51MPam^{1/2}$) were higher than that of 3rd pressed specimen($1.39MPam^{1/2}$). The changes of surface hardness of 1st, 2nd, and 3rd pressed specimens after treatment with fluoric acid were 0.17, 0.06, 0.05 (GPa) respectively, and those of 1st, 2nd, and 3rd pressed specimens after treatment with carbonic acid were 0.07, 0.00, 0.05(MPa) respectively. The pressing accuracy of 1st,2nd and 3rd specimen were 77.22%, 85.681%, and 75.05%. The pressing accuracy of 2nd pressed specimen was higher than that of the 3rd specimen. Conclusion : The changes of physical properties according to recycling of IPS Empress 2 from this study were insignificant. Therefore the possibility of recycling of IPS Empress 2 can be suggested from the results.

• Journal of the Society of Naval Architects of Korea
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• v.61 no.1
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• pp.51-60
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• 2024

Hi Air Korea and Hanwha ocean are currently developing an Onboard Carbon dioxide Capture System (OCCS) to absorb CO₂ emitted from ship's engine using a sodium hydroxide(NaOH) solution, and converting the resulting salt into a solid form through a chemical reaction with calcium oxide (CaO). The system process involves the following steps; 1)The reaction of CO₂ gas absorption in water, 2)The reaction between carbonic acid (H₂CO₃) and NaOH solution to produce carbonate or bicarbonate, and 3)The reaction between carbonate or bicarbonate and CaO to form calcium carbonate (CaCO₃). And ultimately, the solid material, CaCO₃, is separated and discharged using a separator. The OCCS has been installed on an ship and the test results have confirmed significant reduction effects of CO₂ in the ship's exhaust gas. A portion of the exhaust gas emitted from the engine was transferred to the OCCS using a blower. The flow rate of the transferred gas ranged from 800 to 1384 m³/hr, and the CO₂ concentration in the exhaust gas was 5.1 vol% for VLSFO, 3.7 vol% for LNG and a 12 wt% NaOH solution was used. The results showed a CO₂ capture efficiency of approximately 42.5 to 64.1 vol% and the CO₂ capture rate approximately 48.4 to 52.2kg/hr. Additionally, to assess the impact of the discharged CaCO₃on the marine ecosystem, we conducted "marine ecotoxicity test" and performed Computational Fluid Dynamics (CFD) analysis to evaluate the dispersion and dilution of the discharged effluent.

• The Korean Journal of Community Living Science
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• v.18 no.2
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• pp.247-263
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• 2007

This study was complied to analyze the professional agricultural techniques level and to suggest a desirable direction for the professional education system for women farmer. The research was conducted by using in-field surveys and interviews. Data was gathered by questionnaire from 147 women farmer in five specialized crop regions: strawberry, cucumber, rose, apple, and oyster mushroom. The results obtained are as follows. First, the technique levels in computer usage and electronic commerce, machinery usage, pesticide and fertilizer utilization, facility automation, and eco-agricultural cultivation were low. Second, the demand for professional education, evaluated through technique level by standard management diagnosis, was recognized. Areas of concern included: cucumber (temperature control, carbonic acid gas control, grading, funds management), strawberry (light control, soil temperature control, irrigation watering, shipping), rose (temperature control, light control, funds management, cooperated management), oyster mushroom (growth cabinet sterilizer), and apple (flower bud pinching, defloration, fruit thinning, funds management). Based on the results of this study, the following are suggestions for the planning of a professional education system for women farmer. First, it needs to address formal education in marketing, machinery usage, facilities automation, and techniques in pesticide and fertilizer utilization. Second, it needs to be a multi- level program with appropriate terminology at every level which is suitable to each age and ability of women farmer. Third, it needs a more comprehensive manual developed by need analysis of women farmer and a larger lecturer pool for professional education.

• Advances in Traditional Medicine
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• v.9 no.1
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• pp.49-57
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• 2009

The antifertility activity of methanol extract of Cuscuta reflexa Roxb. stem (MECR) and Corchorus olitorius Linn. seed (MECO) were studied on male Swiss albino mice. The extracts were found to decrease sperm count, percentage of motile sperm and testosterone level in treated mice when compared with vehicle control after 17 days of treatment. The weight of gonads, epididymis were decreased whereas no significant changes of the body weight of mice were observed after methanol extract treatments. The fertility test showed 100% negative result in MECR and MECO treated mice at medium and high dose level of treatment. MECR and MECO in low (25 mg/kg and 15 mg/kg, respectively), medium (50 mg/kg and 20 mg/kg, respectively) and high (75 mg/kg and 25 mg/kg, respectively) dose level caused a simultaneous fall in testicular ${\Delta}5$-$3{\beta}$-hydroxy steroid dehydrogenase and glucose-6-phosphate dehydrogenase activities which are involved in testicular steroidogenesis. Total cholesterol and ascorbic acid content in testis were increased significantly in gonads. The activities of lactate dehydrogenase, malic dehydrogenase and ascorbic acid oxidase were reduced whereas that of carbonic anhydrase was increased significantly in the testis of MECR and MECO treated mice. All these observations indicate that the methanol extract of C. reflexa stem and C. olitorius seed produced antifertility activity in sexually matured male mice, which may be due to inhibition of gonadal steroidogenesis. This activity may be attributed due to the presence of flavonoids and steroids, respectively.

• 보존과학연구
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• s.24
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• pp.169-184
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• 2003

Yearly mean temperature and relative-humidity of the Seokguram Grotto was measured $19~23^{\circ}C$, 40~44% from May, 1998 to December,2002. The measurement has little differences comparing to the optimum guideline (temperature : $20^{\circ}\pm2^{\circ}C$, relative-humidity : $50^{\circ}\pm5%$). It is necessary to increase humidity in the Seokguram Grotto during winter because of heating and decrease the temperature during summer because of a higher temperature of outside. In addition, the diurnal range keep in $4^{\circ}C$ of temperature and in 10% of relative-humidity. Yearly mean concentration of $CO_2$(carbon dioxide) was measured538~658ppm that is higher than concentration of normal atmosphere(360 ppm). The $CO_2$ has an cumulative effect on the surface of stone cultural properties as a form of carbonic acid($H_2CO_3$) after reaction with water. HVAC (Heating, Ventilation and Air Conditioning) system should be operated to maintain ideal state for the preservation according to the optimum guideline. Also, the entrance into the Seokguram Grotto should be controlled to prevent a sudden fluctuation of humidity and temperature. Human could carry small particles like a microdust, microbe, etc., into the Seokguram Grotto and also could damage the surface by a direct touch.

• Journal of the Korean institute of surface engineering
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• v.50 no.6
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• pp.531-535
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• 2017

Enormous amount of waste oyster-shell (OS) has a major disposal problem in coastal regions. OSs have attracted much attention for recycling, because these are mainly composed of calcium carbonate with rare impurities. In this study, we demonstrate the calcareous deposit films on steel plate by using OSs on the basic of cathodic protection technique. The composition of the OSs was analyzed by wavelength dispersive X-ray fluorescence spectrometer. Carbon dioxide gas was pumped into distilled water to make carbonic acid solution for dissolution of OS. The calcareous deposit was characterized by second electron microscope (SEM), energy dispersive X-ray analysis (EDX) and X-ray diffraction. Corrosion rates were estimated by measurements of anodic polarization and immersion test. It is confirmed that calcareous deposits on steel plate are formed under all condition of cathodic protection by using waste OS from the SEM and EDX results. Calcareous deposits on steel by OS provide good corrosion resistance by acting as a barrier to oxygen supply to the steel surface.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.47 no.4
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• pp.305-316
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• 2021

This study relates to the development of a new gel sheet mask that finally does not require support based on the reactivity and acid/base reaction experiments of Tamarindus indica seed gum (TG), ethanol, and polyols. When TG and a specific alcohol was mixed at a certain mixing ratio, a transparent gel is formed by reaction with each component, and thus a gel sheet mask without support might be obtained using the mixture. In order to maximize skin tone improvement, a carbonation system of acid and base reactions was introduced, and skin brightness and moisturizing power were evaluated using a spectrophotometer and a moisture measuring device. Through this study, it is expected that the gelation reaction by hydrogen bonding of TG, ethanol, and polyols can be developed into various types, and the gel sheet mask formulation introduced in this study is expected to help develop new products in the future.