• Bulletin of the Korean Chemical Society
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• 제30권2호
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• pp.297-301
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• 2009

We have developed the yttrium oxide (YNP) or ytterbium oxide (YbNP) nanoparticle/polymer matrices for the size-dependent separation of DNA ranging from 100 bp to 9,000 bp. High separation efficiency (> $10^6$ plates/m) and the baseline resolution for various DNA standards (100 bp, 500 bp, and 1 kbp DNA ladder) were obtained in 10 min with these matrices. The effects of concentrations of both polyethylene oxide (PEO) and nanoparticles were investigated and the highest performance was obtained at 0.02% PEO with 0.02% YNP or YbNP. Similar sieving power for both YNP and YbNP matrices was observed probably due to the similar sizes of nanoparticles, resulting in the formation of comparable sieving networks for DNA separation. For the reduction of electrosmotic flow, either dynamic or permanent coating of the capillary inner wall was compared and it turned out that PEO was superior to polyvinylpyrrolidone (PVP) or polyacrylamide (PAA) for better separation efficiency.

• Bulletin of the Korean Chemical Society
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• 제32권4호
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• pp.1361-1364
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• 2011

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVII)
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• pp.622-622
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• 2005

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 2007년도 Proceedings of The Convention
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• pp.32-37
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• 2007

• 한국방사성폐기물학회:학술대회논문집
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• 한국방사성폐기물학회 2017년도 추계학술논문요약집
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• pp.339-340
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• 2017

• 대한약학회:학술대회논문집
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• 대한약학회 2001년도 Proceedings of the Pharmaceutical Society of Korea
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• pp.218.2-218.2
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• 2001

• 대한약학회:학술대회논문집
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• 대한약학회 2000년도 NEW STRATEGY FOR DRUG DEVELOPMENT IN POST-GENOMIC ERA(대한약학회)
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• pp.248.2-248.2
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• 2000

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.220.1-220.1
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• 2002

• 분석과학
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• 제18권1호
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• pp.35-42
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• 2005

절삭폐수의 여과수 등에 많이 포함된 음이온 계면활성제의 정성 및 정량분석을 위한 기초연구로 $Mg^{2+}$ 이온을 포함하는 완충용액에서의 음이온 계면할성제들의 거동을 알아보고 최적의 분석조건을 찾기 위한 실험을 수행하였다. $Mg^{2+}$ 이온과의 상호작용 결과, 탄소수에 무관하게 카르복실레이트 혹은 술포네이트계 음이온계면활성제는 $Mg^{2+}$의 농도가 증가함에 따라 겉보기 이동도가 크게 변하였다. 겉보기 이동도, 전기삼투 이동도 그리고 전기영동 이동도를 계산하여 비교한 결과, 이러한 겉보기 이동도의 변화는 전기 영동 이동도의 영향보다는 주로 전기삼투 이동도의 변화의 영향임을 알 수 있는 근거를 제시하였다.

• KSBB Journal
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• 제27권4호
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• pp.199-206
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• 2012

Chiral crown ethers are synthetic macrocyclic polyethers that bind protonated chiral primary amines with high selectivity and affinity. They have been widely used to separate or distinguish the enantiomers of chiral compounds containing a primary amino moiety by high-performance liquid chromatography, capillary electrophoresis, and NMR spectroscopy. In this paper, two important chiral crown ethers including chiral binaphthyl unit and (18-crown-6)-2,3,11,12-tetracarboxylic acid as chiral selectors are focused. And several chiral resolution techniques and their applications in chirotechnology using these chiral crown ethers with related chiral recognition mechanism studies are reviewed. Especially, it was shown that the commercially available HPLC columns based on (18-crown-6)-2,3,11,12-tetracarboxylic acid have been developed and successfully applied for the resolution of various primary amino compounds including amino acids.