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Spermatogenesis in three Korean shrews and notes on their phylogenetic significance (한국산 땃쥐류 3종의 정장형성 및 이들의 계통 유연관계에 대한 소고찰)

  • 정순정;윤명희
    • Journal of Life Science
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    • v.11 no.3
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    • pp.218-229
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    • 2001
  • The cycle of the seminiferous epithelium and spermiogenesis in three species if the genus Crocidura, the lesser white-toothed shrew, C. suaveolens, the Japanese white-toothed shrew, C. dsinezumi and the big(=Ussuri) white-toothed shrew C. lasiura, in the breeding season were studied with light and electron microscopes. The three species examined are distinguished from each other in the morphology of the seminiferous epithelium and the spermiogenesis, suggesting that these morphological characteristics are useful for the identification of the species. C. dsinezumi and C. lasiura, however, share many characteristics which are not common in C. suaveolens, as follows: In both species, 1) the cycle of the seminiferous epithelium is composed of 10 stages against 11 stages in C. suaveloens; 2) the earliest intermediated type spermatogonia is observed at stage I against stage III in C. suaveolens; 3) the spermatids of step 5 is observed during the stages V-VI against stages V-VII in C. suaveolens; 4) the acrosomal extension occurs during the stages VIII-X against tages IX-XI in C. suaveolens; 5) the condensation of the nucleus occurs simultaneously whereas it begins from the middle and along the nuclear membrane in C. suaveolens; 6) the capitular length in acrosome phase is shorter(about 2/3 of the diameter of the proximal centriole) than in C. suaveolens(longer than the diameter of proximal centriole; 7) length of the post nuclear cap is shorter(less than a half of the nucleus) than in C. suaveolens(about a half on the nucleus). Hudging from the similarities in the spermatogenesis in C. dsinezumi and C. lasiura, the relationship between them seems to be close compared to those with C. suaveolens.

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Localization of Translation Initiation Factors to the Postsynaptic Sites (신경세포 연접후 위치에 단백질합성 해석시작인자(eIF)들의 존재)

  • Choi, Myoung-Kwon;Park, Sung-Dong;Park, In-Sick;Moon, Il-Soo
    • Journal of Life Science
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    • v.21 no.11
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    • pp.1526-1531
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    • 2011
  • Local protein synthesis in neuronal dendrites is important for site-specific regulation of synaptic plasticity. In this study, we investigated whether translation initiation factors (eIFs) are present at the postsynaptic sites. High resolution confocal microscopy showed that the eIF4E and eIF4G (which bind the 5'-terminal mRNA cap), eIF5 (which is important during the 3' direction scanning to find an initiation codon), eIF6 (which mediates upregulation of translation by external stimuli), and eIF5A (which mediate translation upregulation under adverse conditions) were localized to the post-synaptic sites. Immunoblot and detergent extraction experiments also indicated that these eIFs were present in the synapse in association with the postsynaptic density (PSD). Our data provide evidence for the strategic positioning of eIFs at the postsynaptic site for initiation of translation in diverse situations.

Behavior of Pile Groups in Multi-layers Soil under Lateral Loading (다층지반에서 횡하중을 받는 군말뚝의 거동)

  • Kim, Yongmoon;Ahn, Kwangkuk
    • Journal of the Korean GEO-environmental Society
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    • v.13 no.3
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    • pp.85-90
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    • 2012
  • This paper deals with the results for a numerical analysis of single piles and pile groups in multi-layers soil(granite soil-clay-granite soil) subjected to monotonous lateral loading using the ABAQUS finite element software. The investigated variables in this study include free head and embedded capped single pile, pile diameter (0.5m), pile length (10m), and pile groups. Numerical analyses were conducted by variation of spacing piles(s=3D, 4D, 5D) to compare the behaviour of single pile without cap and group pile. The $1{\times}3$ pile group(leading pile, middle pile, trail pile) was selected to investigate the individual pile and group lateral resistance, the distribution of the resistance among the piles. The analysis model of clay and the material of granite soil was modeled by using Druker-Prager constitutive relationship and existing treatise respectively. The pile was considered as a elastic circular concrete pile. As a result, the more pile space was extended, the value of P-multiplier is appeared to be less effective to leading pile. The lateral resistance of single-layer showed approximately 4-20% larger than the multi-layers.

In vitro evaluation of fracture strength of zirconia restoration veneered with various ceramic materials

  • Choi, Yu-Sung;Kim, Sung-Hun;Lee, Jai-Bong;Han, Jung-Suk;Yeo, In-Sung
    • The Journal of Advanced Prosthodontics
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    • v.4 no.3
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    • pp.162-169
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    • 2012
  • PURPOSE. Fracture of the veneering material of zirconia restorations frequently occurs in clinical situations. The purpose of this in vitro study was to compare the fracture strengths of zirconia crowns veneered with various ceramic materials by various techniques. MATERIALS AND METHODS. A 1.2 mm, $360^{\circ}$ chamfer preparation and occlusal reduction of 2 mm were performed on a first mandibular molar, and 45 model dies were fabricated in a titanium alloy by CAD/CAM system. Forty-five zirconia copings were fabricated and divided into three groups. In the first group (LT) zirconia copings were veneered with feldspathic porcelain by the layering technique. In the second group (HT) the glass ceramic was heat-pressed on the zirconia coping, and for the third group (ST) a CAD/CAM-fabricated high-strength anatomically shaped veneering cap was sintered onto the zirconia coping. All crowns were cemented onto their titanium dies with Rely $X^{TM}$ Unicem (3M ESPE) and loaded with a universal testing machine (Instron 5583) until failure. The mean fracture values were compared by an one-way ANOVA and a multiple comparison post-hoc test (${\alpha}$= 0.05). Scanning electron microscope was used to investigate the fractured interface. RESULTS. Mean fracture load and standard deviation was $4263.8{\pm}1110.8$ N for Group LT, $5070.8{\pm}1016.4$ for Group HT and $6242.0{\pm}1759.5$ N for Group ST. The values of Group ST were significantly higher than those of the other groups. CONCLUSION. Zirconia crowns veneered with CAD/CAM generated glass ceramics by the sintering technique are superior to those veneered with feldspathic porcelain by the layering technique or veneered with glass ceramics by the heat-pressing technique in terms of fracture strength.

On a Modified Structure of Taper Type Planar Power Divider/Combiner at 2 GHz (2 GHz 평면 테이퍼형 전력 분배/결합회로의 수정된 구조 연구)

  • 한용인;김인석
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.13 no.10
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    • pp.1005-1016
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    • 2002
  • In this paper, a 2 GHz tapered shape of multiport power divider/combiner modified from the model published by [10] and adopted PBG(Photonic Band Gap) structure is proposed. Parameters determining electrical property of the circuit structure have been analyzed by HFSS simulation. For input matching, balance of output signals and phase linearity at each output port, one circular hole has been etched out on the circuit surface. 1:2 and 1:3 power dividers/combiners designed by this study have been compared with the same circuits designed by the method of [10] in terms of S-parameters. As a result, it has been found that tile modified structure and PBG of power divider/combiner have improved return loss more than 20 dB and another 18 dB. respectively, at 2 GHz.

Selective Epitaxial Growth of Si and SiGe using Si-Ge-H-CI System for Self-Aligned HBT Applications (Si-Ge-H-CI 계를 이용한 자기정렬 HBT용 Si 및 SiGe 의 선택적 에피성장)

  • Kim, Sang-Hoon;Shim, Kyu-Hwan;Kang, Jin-Young
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2002.11a
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    • pp.182-185
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    • 2002
  • 자기정렬구조의 실리콘-게르마늄 이종접합 트랜지스터에서 $f_{max}$를 높이기 위한 방안으로 베이스의 저항 값을 감소시키고자 외부 베이스에 실리콘 및 실리콘-게르마늄 박막을 저온에서 선택적으로 성장할 수 있는 방법을 연구하였다. RPCVD를 이용하여 $SiH_{2}Cl_{2}$$GeH_{4}$를 소스 가스로 하고 HCI을 첨가하여 선택성을 향상시킴으로써 $675\sim725^{\circ}C$의 저온에서도 실리콘 및 실리콘-게르마늄의 선택적 에피성장이 가능하였다. 고온 공정에 주로 이용되는 $SiH_{2}Cl_{2}$를 이용한 실리콘 증착은 $675^{\circ}C$에서 열분해가 잘 이루어지지 않고 HCl의 첨가에 의한 식각반응이 동시에 진행되어 실리콘 기판에서도 증착이 진행되지 않으나 $700^{\circ}C$ 이상에서는 HCI을 첨가한 경우에 한해서 선택성이 유지되면서 실리콘의 성장이 이루어졌다, 반면 실리콘-게르마늄막은 실리콘에 비해 열분해 온도가 낮고 GeO를 형성하여 잠입시간을 지연하는 효과가 있는 게르마늄의 특성으로 인해 선택성이나 증착속도 모두에서 유리하였으나 실리사이드 공정시에 표면으로 게르마늄이 석출되는 현상 등의 저항성분이 크게 작용하여 실리콘-게르마늄막 만으로는 외부 베이스에의 적용은 적절하지 않았다. 그러나 실리콘막을 실리콘-게르마늄막 위에 Cap 층으로 증착하거나 실리콘막 만으로 외부 베이스에 선택적으로 증착하여 베이스의 저항을 70% 가량 감소시킬 수 있었다.

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Germ cell Differentiation During Spermatogenesis, and Ultrastructural Characteristics of Mature Sperm in Male Phacosoma japonicus (Bivalvia: Veneridae)

  • Kim, Jin-Hee;Chung, Ee-Yung;Choi, Moon-Sul;Lee, Ki-Young;Lee, IL-Ho;Seo, Won-Jae
    • The Korean Journal of Malacology
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    • v.27 no.1
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    • pp.55-65
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    • 2011
  • Some characteristics of germ cell differntiations during spermiogenesis and mature sperm ultrastructure in male Phacosoma japonicus were investigated by transmission electron microscope observations. The morphology of the spermatozoon of this species has a primitive type and is similar to those of other species in the subclass Heterodonta. Morphologies of the sperm nucleus and the acrosome of this species are the cylindrical type and cap shape, respectively. The spermatozoon is approximately 45-50 ${\mu}m$ in length, including a long curved sperm nucleus (about $3.70{\mu}m$ long with 45 $^{\circ}$ of the angle of the nucleus, an acrosome (about $0.55{\mu}m$ in length), and tail flagellum (about 42-$47{\mu}m$)The axoneme of the sperm tail shows a 9+2 structure. As some characteristics of the acrosomal vesicle structures, the basal and lateral parts of basal rings show electron opaque part (region), while the anterior apex part of the acrosomal vesicle shows electron lucent part (region). These characteristics of the acrosomal vesicle were found in the family Veneridae and other several families in the subclass Heterodonta. These common characteristics of the acrosomal vesicle in the subclass Heterodonta can be used for phylogenetic and systematic analysis as a taxonomic key or a significant tool. The number of mitochondria in the sperm midpiece of this species are four, as one of common characteristics appear in most species in the family Veneridae and other families in the subclass Heterodonta. However, exceptionally, only three species in Veneridae of the subclass Heterodonta contain 5 mitochondria. The number of mitochondria in the sperm midpiece can be used for the taxonomic analysis of the family or superfamily levels as a systematic key or tools.

Analysis of LCOE for Korean Onshore Wind Farm Considering Social Discount Rate (사회적 할인율을 고려한 국내육상풍력발전 단지의 LCOE 분석)

  • Lee, Keon-Woo;Ko, Kyung-Nam
    • Journal of the Korean Solar Energy Society
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    • v.40 no.1
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    • pp.1-13
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    • 2020
  • A study on estimation of the Levelized Cost of Energy (LCOE) was conducted for the Korean onshore wind farms. The LCOE was estimated on the basis of the actual wind farm data from Data Analysis, Retrieval Transfer system (DART) run by Financial Supervisory Service. Recently, social discount rate of Korea dropped from 5.5% to 4.5%, which was taken into account for this study. The onshore wind farms studied accounted for 42% of all the onshore wind farms of South Korea. Capital Expenditure (CapEx) and Operation Expenditure (OpEx) were calculated from the actual data, while Capacity Factors (CFs) were obtained from the wind farms of five provinces. Their distributions were estimated using Maximum Likelihood Estimation method, and then Monte Carlo Simulation (MCS) was performed for estimating LCOE, Levelized Fixed Cost (LFC), and Levelized Variable Cost (LVC). As a result, the LCOEs at the two discount rates, 4.5 and 5.5%, were 142 and 152 $/MWh, respectively, which were lower than that of financially viable onshore wind project of Korea. The 1% drop of social discount rate was estimated to result in a 10 $/MWh decrease in LCOE and a 4 $/MWh in LFC, which can be an advantage for wind project investors.

The Formulation and Dissolution Properties of Oral Sustained Release Sulindac Delivery System (설린닥의 경구용 지속성 제제설계 및 용출특성)

  • Rhee, Gye-Ju;Park, Sun-Hee;Suh, Sung-Su;Whang, Sung-Joo
    • YAKHAK HOEJI
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    • v.41 no.1
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    • pp.48-59
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    • 1997
  • Sustained release matrix tablets, pellets, and coated pellets for the delivery of sulindac were prepared using cellulose derivatives at various ratios, and evaluated for the dis solution pattern. The release of sulindac, from matrix tablets prepared with low viscosity HPMC was relatively fast, and especially the tablets made of Metolose SM released all of sulindac within 1 hr. The release of drug from tablets made of other HPMC derivatives were retarded in the order of the following: Pharmacoat 645>Pharmacoat 606>Pharrnacoat 606+HPC-L>HPC-L. The most sustained release pattern was observed with the preparation of high viscous polymer. Metolose 90 SH. While release of sulindac, from matrix type pellet containing 10mg/cap of Metolose 90 SH or 60 SH was completed within 1 hr, a prolonged release formulation (30% in 1 hr) was obtained by the inclusion of EC. Pellets coated with HPMC showed a fast release pattern (${\geq}$ 80% within 2 hrs), whereas pellets coated with HPMC and EC (molar ratio 1 : 1) showed a sustained release pattern (${\geq}$ 80% in 12 hrs), vath the release from EC pellets being the most sustained. Fast (naked) and slow release pellets coated with EC, Metolose 60SH 50cps and propylene glycol. and enteric pellets coated with HPMCP 55 and Myvacet$^{\circledR}$ were prepared, and combined at various ratios for the assessment of dissolution pattern. The result indicates the possibility that the development of 24 hr sustained release delivery systems containing sulindac for oral administration could be achieved by means of combining sustained and fast release pellets at a proper portion.

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Analysis and measurement of low frequency magnetic field according to internal position of electric railway train (전기철도차량 객실 내부 위치에 따른 극저주파 자계 측정 및 분석)

  • Jang, Dong-Uk;Han, Moon-Seob
    • Proceedings of the KSR Conference
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    • 2008.06a
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    • pp.489-494
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    • 2008
  • The measurement of magnetic field is performed about DC and AC magnetic field in electric railway line. The test point is cap, on the converter/inverter box, on the traction motor and on the SIV, the height of measurement is bottom and 60 cm height. In case of AC magnetic field, the selected specific frequency is measured on the converter/inverter box. The AC magnetic field is checked and analysis through BNC output, DAQ cad and notebook PC.

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