• Applied Biological Chemistry
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• v.27
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• pp.29-46
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• 1984

To utilize several species of hard wood as raw materials of feed products, fermentation characteristics of cellulosic substrates to single cell protein was investigated, and results were summarized as follows. Among the microorganisms investigated, Tricoderma viride was selected as one of the most cellulolytic. Mixed culture of fungi did not show a synergistic effect on cellulose degradation. When the fungi were cultured at $28^{\circ}C$ for 7 days in a medium containing wheat bran 25 g, cellulose 0.25 g, proteose peptone 0.025 g and tween 800.025 g, cellulotic activities on carboxy methyl cellulose and filter paper reached maximum at 12 hr. The alkali treatment resulted in increased degradation of substrate from 13 to 18% when treated with enzymes for 12h, and reducing sugar formation increased with decreased size of substrates. Glucose was a very good feedback inhibitor of the enzyme from T.viride than that of xylose. When the substrate was rehydrolyzed, hydrolysis rate was 31% to reducing sugars within 12 hr. Quantative anlysis with HPLC showed the ratio of glucose to xylose in sugar syrups as 1.77 to 1. For the purpose of producing cellulosic-single cell protein from the sawdust of mulberry tree, 15 strains of xylose-assimilating yeast were isolated from 42 samples of rotten woods and compost soils and examined for their ability to utilize xylose. Then three strains were selected by their strong xylose-assimilating activities. The cultivative condition, the growth characteristics, and protein and nucleic acid productivities of three strains were investigated. The results obtained were, 1. Wood hydrolysate of mulberry tree was assimilated by 5 strains of CHS-2, CHS-3, ST-40, CHS-12 and CHS-13. 2. The optimum initial pH and temperature for the growth of strain CHS-13 were 4.4 and $30^{\circ}C$. 3. The specific growth rate of strain CHS-13 was $0.23h^{-1}$ and generation time was 3.01 hrs at the optimum condition. 4. CHS-13 strain assimilated 81 % of sugar in wood hydrolysate. 5. CHS-13 strain was identified as Candida guilliermondii var. guilliermondii 6. When the CHS-13 strain was cultured in the wood hydrolysate containing yeast extract, L-protein content was increased with yeast extract concentration. 7. The L-protein and nucleic acid yields from wood hydrolysate were 0.73 mg/ml and $4.92{\times}10^{-2}\;mg/ml$ respectively. 8. An optimal nucleic acid content of CHS-13 strain was observed in the medium containing 0.2% of yeast extract.

• Food Science and Biotechnology
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• v.14 no.4
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• pp.474-478
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• 2005

Because it possesses anti-inflammatory, antifungal, antiviral, and tissue regenerative properties, propolis has been used for thousands of years in folk medicine for multiple purposes. Although the antimicrobial activity of propolis has already been demonstrated, very few studies have been conducted on bacteria of clinical relevance in dentistry. The aim of this study is to evaluate the antimicrobial, anti-inflammatory, and anti-oxidative activities of 0.1% and 1.0% propolis, both of water-extracted (proAQ) and ethanol-extracted (proAL) propolis, for industrial applications. In studies of antimicrobial activity, the growth of Staphylococcus aureus ATCC 35556, Salmonella enteritidis ATCC 12021, Escherichia coli O157:H7, and Candida parapsilosis KCCM 35428, all general food or clinical pathogens, were tested. The culture medium used was trypticase soy broth including 0.6% yeast extract; after 6 hr of incubation, the turbidities were measured at 620 nm with a spectrophotometer. The results indicate that the antimicrobial effects of both 1.0% proAQ and 1.0% proAL were greater against the growth of S. aureus ATCC 35556 and C. parapsilosis KCCM 35428 rather than those of S. enteritidis ATCC 12021 and E. coli O157:H7. Additionally, it appears that the anti-inflammatory effects of proAL are greater than those of proAQ. The anti-inflammatory effects were evaluated by measurement of the inhibition of hyaluronidase activity in vitro. At a 1% concentration, the anti-inflammatory effects of proAL were greater than those of proAQ. Finally, the anti-oxidative effects of 1% and 10% solutions of each extract sample were measured according to the TBA method at $40^{\circ}C$ for 1, 2, 3, and 5 days and were compared with 1.0% BHT. The results indicate that the anti-oxidative effects at 0.1% for both proAQ and proAL were not significantly different than the anti-oxidative effects at 1.0% BHT (p<0.05). Thus, it appeared that the alcohol-extracted propolis had greater antimicrobial, anti-inflammatory, and anti-oxidative effects than the water-extracted propolis. This is based on the presumption that major biofunctional components were fat-soluble, rather than water-soluble.

• 보존과학연구
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• s.5
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• pp.148-166
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• 1984

The Investigation of organisms damaged to papers and cellulose materials of Cultural Property in the Ch'ang Dok Palace The investigation of the airborne fungi, the attached fungi to the papers and cellulose materials of Cultural Properties in addition to the insects inhabiting at the Ku Sonwon Jon, Shin Sonwon Jon and Yonwa ch'anggo in the Ch'ang Dok Palace carried out from Jul. 10 to Jul. 21,1984.The results are summarized as follows ;1. Isolation and identification of the airborne fungi from the three storages were Cladosporium sp., Alternaria alternata, As pergillus cervinus, A. flavus, A. nidulance, A. oryzae, A. terreus, A. versicolor, A. wentii, Penicillium adametzii, P.albicans, P.canescens, P. citreo-viride, P. citrinums, P. decumbens, P. frequentans, P. funiculosum, P.herquei, P.implicatum, P.multicolor, P.nigricans, P.nonatum,P.purpurogenum, P.roqueforti, P.viridicatum, Trichodema viride, Geotrichumcandidum, Curvvlaria lunata, Torula hebarum, T.thermophila, Itersoniliasalmonicolor, Drechsclera avenue, Candida sp., Acremonium sp., and Botrytis sp., It was found that thirty five species in thirteen genera was isolated. Among them, the dominant species was Cladosporium sp., and the order was Penicillium, Aspergillus, Alternaria and so on.2. The attached fungi directly isolated from the papers and cellulose materials of Cultural Properties were twenty-nine species in fourteen genera, namely, Acremonium sp., Albertiniella sp., Alternaria alternata, Aspergillus clavatus,A.niger, A.ornatus, A.versicolor, Botrytis sp., Bysochlamys sp., Carpenteles sp.,Chaetomium globosum, Cladosporium sp., Eurotium sp., Mucor sp., Penicilliumcanescens, P.chermesium, P.citrinum, P.frequentans, P.funiculosum, P.herqueiP .implicatum, P.javanicum, P.luteum, P.purpurogenum, P.thomii, P.viridicatum, Torula thermophila, Trichoderma koningi and T.viride. Among them, the mostfungi distributed on the surface of the papers and cellulose materials was Penicillium and the order was Aspergillus, Alternaria, Cladosporium, Trichodermaand so on.3. The insects collected the three storages were ten genera and ten species including 916 specimens. By classifying the insects collected, the most species of the insects was Stenoscelodes hayashii of 857 specimens occupied about 93% of the total insect. And the other insects were collected as Microgamme costipennisAnobium pertinax, Xenomimetes alni, Anthrenus verbasci, Holoparmecus signatus,Thermobia domestica, Halyomorpha brevis, Drosophila coracine and Brattaorientalis. As described above, it could be known that the most airborne fungi was Cladosporium and the order was Penicillium, Aspergillus, Alternaria in the three storages. And the most attached fungi distributed on the surface of papers and cellulose materials was Penicillium and the other fungi were Aspergillus, Alternaria Cladosporium, Trichoderma and so on. Accordingly, from the results, itwas assumed that the major part of airborne fungi were attached to the papers and cellulose materials of Cultural Properties. The paper and cellulose materials of Cultural Properties in Ch'ang Dok Palace were chiefly damaged by S.hayashii in Coleoptera.

• Korean Journal of Food Science and Technology
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• v.48 no.3
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• pp.241-246
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• 2016

The object of this study was to develop an alternative way to treat human skin pathogens using marine algae. During this study, we observed that the ethanolic extract of the edible brown algae [Sargassum serratifolium (C. Agardh) C. Agardh] exhibited potential antimicrobial activity against pathogenic commensal bacteria related with acne vulgaris (Propionibacterium acnes, Staphylococcus epidermidis, Staphylococcus aureus and Pseudomonas aeruginosa), and Candida albicans which causes cutaneous candidiasis. Among the solvent-soluble fractions from the ethanolic extract, a hexane-soluble fraction showed the strongest antimicrobial activity against all tested human skin pathogens with MIC values ranging from 32 to $512{\mu}g/mL$. In addition, the hexane fraction exhibited a synergistic antimicrobial activity with commercial antibiotics used in the treatment of acne vulgaris or cutaneous candidiasis. Thus, this study suggests that S. serratifolium extract could be a potential source of natural antimicrobial agents or a pharmaceutical component against human skin pathogens.

• Korean Journal of Environmental Agriculture
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• v.34 no.2
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• pp.149-154
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• 2015

BACKGROUND: Yeast isolates associated with the leaves, stems, and flowers of the tiger lily needed to be identified using isolation methods that have previously been used effectively in yeast biotechnology. A culture-based approach was necessary for the isolation of many yeast strains associated with tiger lily. METHODS AND RESULTS: In this study, the homogenized leaves, stems, and flowers of tiger lily were spreaded onto GPY medium containing chloramphenicol, streptomycin, Triton X-100, and L-sorbose. A total of 82 yeast strains from the leaves, 94 and 97 yeast strains from the stems and flowers were isolated, respectively. Yeast isolates were identified by phylogenetic analysis based on internal transcribed spacer region sequencing. The yeast species isolated from the leaves comprised of 31 isolates of the genus Pseudozyma, 28 of Aureobasidium pullulans, and 11 of the genus Cryptococcus. Those isolated from the stems comprised of 40 of A. pullulans and 11 of Cryptococcus, and 95 of A. pullulans While, 1 isolate each of the genera Rhodotorula and Metschnikowia were isolated from the flowers. CONCLUSION: We identified site-specific yeast communities associated with tiger lily. These yeast isolates may have high potential for application in the field of biotechnology.

• Microbiology and Biotechnology Letters
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• v.39 no.1
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• pp.37-42
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• 2011

I isolated the actinomycete strain KH223 from soil samples collected from the Kye Ryong mountain area. This strain is antagonistic to the multidrug-resistant Acinetobacter baumannii. KH223 was confirmed as belonging to the genus Streptomyces based on the scanning electronmicroscopy(SEM) observations of the diaminopimelicacid(DAP) type and morphological and physiological characteristics. Comparison of the 16S rDNA nucleotide sequences revealed that KH223 has a relationship with Streptomyces galbus. Production of antibiotics by KH223 was most favorable when cultured on a glucose, polypeptone, and yeast extract(PY) medium for 6 days at 27$^{\circ}C$. The supernatant was found to exhibit an antimicrobial effect on various kinds of bacteria and fungi. Particularly, butanol and ethylacetate extracts of KH223 and cyclo(trp-trp) exhibited significant activity against A. baumannii at concentration ranges of 0.8-12.5 ${\mu}g$/mL, 5.0-25 ${\mu}g$/mL and 12.5${\rightarrow}$100 ${\mu}g$/mL, respectively. Moreover, in contrast to cyclo(trp-trp) had shown to activity against Micrococcus luteus JCM 1464 at the concentration of 12.5 ${\mu}g$/mL, the butanol extract of KH223 showed significant activity against Bacillus subtilis IAM 1069 and Micrococcus luteus JCM 1464 at the concentration of 0.4 and 0.8 ${\mu}g$/mL, respectively. These results suggest that KH223 may have a great potential in the production of new antibiotics to combat multidrug-resistant pathogens and further studies may be warranted for the same.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.37 no.4
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• pp.303-308
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• 2011

Lemon and eucalyptus oils were known to have various biological effects such as anti-aging and sterilizing action. However these essential oils were not well studied about their antioxidant activity and antimicrobial activity against specific skin flora. In this study, we investigated antioxidant activity and antimicrobial activity of lemon and eucalyptus oils by using DPPH radical scavening activity and paper disc method. Lemon oil showed the high DPPH scavenging activity, while eucalyptus oil did not. Blending oils of lemon and eucalyptus had little enhancememt onantioxidant activity. Paper disc method demonstrated that each oil had a high antimicrobial effect against C. albicans and P. acnes in a concentration dependent manner. The blending oils had enhancememt on antimicrobial effect against P. acnes. In conclusion, the blending oil of lemon and eucalyptus can be used as a more effective natural agents for cure of skin trouble and acne.

• Journal of Food Hygiene and Safety
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• v.24 no.3
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• pp.273-276
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• 2009

We investigated eight active natural antimicrobials for preservation of functional beverages that are usually degraded by yeasts rather than by bacteria due to a high sugar content and a low pH. Five strains of yeasts (S. cerevisiae, Z. bailii, P. membranaefaciens, C. albicans, and P Anomala) were tested with eight natural antimicrobial agents ($\varepsilon$-polylysine, yucca extract, vitamin $B_1$ derivative, scutellaria baicalensis extract, chitooligosaccharid, allyl isothiocyanate, sucrose-fatty acid ester, and oligosaccharide). The lowest minimal inhibitory concentrations (MIC) were 10 ppm for oligosaccharide and sucrose-fatty acid ester against S. cerevisiae and Z. bailii, 10 ppm for allyl isothiocynate against P. membranaefaciens and C. albican, and 10 ppm for allyl isothiocynate and oligosaccharide against P. anomala. No growth were observed for five kinds of yeasts in functional beverages containing sodium benzonate at concentration of 0.015% or higher. The resistance of S. cerevisiae, Z. bailii, and P. Anomala against natural antimicrobial agents was lower than those of P. membranaefaciens and C. albican. Allyl isothiocyanate, oligosaccharide, and sucrose-fatty acid ester showed the highest antimicrobial activities among the eight tested antimicrobials. These results can be applied to develop new natural antimicrobial agents to improve microbial quality of functional beverages.

• 한국생물공학회:학술대회논문집
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• 2000.04a
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• pp.53-56
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• 2000

Two-stage fed-batch culture was peformed to improve the volumetric productivity of erythritol. In the growth phase dissolved oxygen was maintained to 20% and the feed medium was automatically supplied to the fermenter by pH-stat mode. The cell yield was 0.76 g-cell/g-glucose. In two-stage fed-batch culture, 41% of total erythritol conversion yield with 187 g/L of erythritol concentration and 2.79 g/L-h of maximum erythritol Productivity were obtained when 400 g/L of glucose was directly added in the form of non-sterile powder at production phase. The erythritol productivity increased in parallel with cell mass. The metabolic shift in the biosynthetic pathway of erythritol was caused by dissolved oxygen concentration. The production of gluconic acid was observed when the dissolved oxygen in the medium was maintained over 40% during the production phase, whereas the dissolved oxygen concentration lower than 40% caused the production of citric acid. But the butyric acid was produced independently with dissolved oxygen concentration in the medium. The production of organic acids such as gluconic acid, citric acid, and butyric acid was decreased by addition of mineral salts.

• Journal of Life Science
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• v.18 no.2
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• pp.228-233
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• 2008

Antimicrobial activities of ethanol extracts of about 60 endemic Korean herbs and medicinal plants were investigated. When compared to the group treated with phenoxyethanol as a positive control, the growth of Pseudomonas aeruginosa was inhibited by Styrax japonica, Stevia rebaudiana, Morus Leaf, Coptis chinensis and Slavia miltorrhiza Bunge, the latter showing the strongest inhibition (97%). The growth of Staphylococcus aureus was inhibited by Acorus calamus, Pinus densiflora, Ginkgo biloba, Dryopteris crassirhizoma, Chrysanthemum zawadskii, Slavia miltorrhiza Bunge and Coptis chinensis, the latter showing the strongest inhibition (99%). The growth of yeast type fungi Candida albicans was inhibited to about 35% by Scutellaria baicalensis Geogr and about 33% by Coptis chinensis as determined by paper disk method. The growth of bacterium Malassezia furfur was inhibited by Paeonia lactiflora, Rosa multiflora, Coptis chinensis, Citrus aurantium L. subsp, Paeonia suffruticosa Andrews, and Galla rhois, the latter showing the strongest inhibition (57%). The growth of Propionibacterium acnes was inhibited by Galla rhois, Paeonia lactiflora, Morus bombycis, Cucumis sativus, Stevia rebaudiana, Coptis chinensis and Slavia miltorrhiza Bunge. Interestingly, Coptis chinensis and Slavia miltorrhiza Bunge showed the strongest inhibition (99%) similar to that in postive control. The possible components and their action modes were suggested and discussed.