• Title/Summary/Keyword: callus type

Search Result 100, Processing Time 0.029 seconds

Efficient Plant Regeneration Using Mature Seed-Derived Callus in Zoysiagrass (Zoysia japonica Steud.) (성숙종자 유래 캘러스를 이용한 들잔디 (Zoysia japonica Steud.)의 효과적인 식물체 재분화)

  • ;TOHYAMA, kohichi
    • Korean Journal of Plant Tissue Culture
    • /
    • v.28 no.2
    • /
    • pp.61-67
    • /
    • 2001
  • Using mature seed-derived callus, optimal conditions for efficient callus growth and plant regeneration, and regeneration efficiency by callus type were investigated in zoysiagrass (Zoysia japonica steud.). Callus induction was highest when the seeds were cultured on MS medium containing 2 mg/L 2,4-D, 0.2 mg/L BAP, 4 mg/L thiamine-HCl and 100 mg/L $\alpha$-ketoglutaric acid. Callus growth was highest when callus were cultured on MS medium containing 0.5 mg/L 2,4-D, 0.05 mg/L BAP, 4 mg/L thiamine-HCl and 100 mg/L $\alpha$-ketoglutaric acid. Plant regeneration was highest when callus was transferred on MS medium containing 3% maltose and 1 mg/L BAP, or 1 mg/L thidiazuron (TDZ). The combinations and concentrations of 2,4-D and BAP were shown to be critical factors for both the frequency and the type of callus. And four morphologically distinct types of callus were induced from the 2,4-D and BAP treatment. Type I,II and III calli produced shoots upon subculture, while the watery callus, type IV produced roots without shoots. Of four types of callus, type I exhibited the maximum frequency (82%) of shoot regeneration and the minimum frequency (4%) of albinism.

  • PDF

Relationship of Foot Type to Callus Location in Healthy Subjects

  • Jung, Do-Young;Kim, Moon-Hwan;Chang, In-Su
    • Physical Therapy Korea
    • /
    • v.13 no.4
    • /
    • pp.64-70
    • /
    • 2006
  • The purpose of this study was to determine whether a relationship existed between foot type and the location of plantar callus in healthy subjects. Twenty-five healthy subjects with plantar callus were recruited for this study. Foot deformities were classified according to the operational definitions as 1) a compensated forefoot varus, 2) an uncompensated forefoot varus or forefoot valgus, or 3) a compensated rearfoot varus. The location of plantar callus was divided into two regions. Fourteen of the 19 feet with compensated forefoot varus and six of the 9 feet showed plantar callus at the second, third or fourth metatarsal head. Five of the 6 feet with uncompensated forefoot varus and twenty of the 16 feet with forefoot valgus showed plantar callus at the first or fifth metatarsal head. A significant relationship was found between foot type and location of callus (p<.01). The results support the hypothesis that certain foot types are associated with characteristic patterns of pressure distribution and callus formation. We believe diabetic patients with insensitive feet and with the types of foot deformity should be fit with foot orthoses and footwears that accommodate their respective deformity in a position as near to the subtalar joint as possible with the goal of preventing plantar ulceration.

  • PDF

Plant Regeneration and Transformation of Kentucky Bluegrass(Poa pratensis L.) via the Plant Tissue Culture (조직배양을 이용한 Kentucky bluegrass(Poa pratensis L.)의 식물체 재분화 및 형질전화 조건의 검토)

  • Miki Kusano;Koichi Tohyama;Bae, Chang-Hyu;Riu, Key-Zyung;Lee, Hyo-Yeon
    • Journal of Plant Biotechnology
    • /
    • v.30 no.2
    • /
    • pp.115-121
    • /
    • 2003
  • In this study, plant regeneration and Agrobacterium tumefaciens-mediated transformation Kentucky bluegrass(Poa pratensis L.) were evaluated. Three different types of calli were produced depending on the combinations of growth regulators. They were non-friable brown or gray-colored callus (type I), compact, friable and yellow or white-colored callus (typeII), and soft, watery translucent callus with differentiated structure (typeIII). The highest regenerable organogenic callus (typeII) was obtained on the medium containing 1mg/L, 2,4-D and 0.1mg/L BA. Additionally, the production of typeII calli increased significantly when AgNO$_3$ was added to the callus induction and growth medium. The highest frequency of multiple shout formation from typeII callus was obtained on MS medium containing 1mg/L BA and 1mg/L Thidiazuron(TDZ). The organogenic calli(typeII) were inoculated with Agrobacterium tumefaciens strain EHA101 harboring the binary vector pIG121Hm with $\beta$-glucuronidase gene, and various factors were found to influence the transfer-DNA delivery efficiency. The highest transient GUS activity was observed on typeIIcallus. In the present work, we reported the first transient GUS activity of Kentucky bluegrass mediated by Agrobacterium tumefaciens. Our system may contribute to genetic improvement for breed-recalcirtrant grass species, Kentucky bluegrass.

Establishment of a novel plant regeneration system from suspension-derived callus in the halophytic Leymus chinensis (Trin.)

  • Sun, Yan-Lin;Hong, Soon-Kwan
    • Journal of Plant Biotechnology
    • /
    • v.37 no.2
    • /
    • pp.228-235
    • /
    • 2010
  • The establishment of cell suspension culture and plant regeneration of the halophytic Leymus chinensis (Trin.) are described in this study for the first time. Callus induction solid medium containing Murashige and Shoog (MS) basic salt, $2.0\;mg\;l^{-1}$ 2,4-dichlorophenoxyacetic acid (2,4-D), and $5.0\;mg\;l^{-1}$ L-glutamic acid with $30.0\;g\;l^{-1}$ sucrose and $4.0\;g\;l^{-1}$ gelrite for solidification induced the highest rate of cell division in Type 1 callus among calli of various types. Liquid medium with the same hormone distribution was therefore, used for cell suspension culture from Type 1 callus. Over a 30 d suspension culture at 100 rpm, great amounts of biomass were accumulated, with 71.07% average daily increment and 22.32-fold total fresh weight increment. Comparison of before and after suspension culture, the distribution of different size callus pieces and the maintenance of callus type were basically unaltered, but a slight increase in relative water contents was observed. To induce the potential of plant regeneration, the directly transferring on plant regeneration solid medium containing MS basic salt, $0.2\;mg\;l^{-1}$ $\alpha$-naphthalene acetic acid (NAA), $2.0\;mg\;l^{-1}$ kinetin (Kn), and $2.0\;g\;l^{-1}$ casamino acid and indirectly transferring were simultaneously performed. Even now growth rates of suspension-derived callus on solid medium were approximately half of those of Type 1 callus, but faster somatic embryogenesis was observed. Rooting of all regenerated shoots was successfully performed on half-strength MS medium. All plants appeared phenotypically normal.

Studies on the Transformation of Crop Plants. IV. Biochemical Characteristics of Embryogenic Callus in Rice (곡물류의 형질전환 유도에 관한 연구 (IV) 벼 배발생 세포의 생화학적 특징)

  • 정병균
    • Journal of Plant Biology
    • /
    • v.36 no.4
    • /
    • pp.377-382
    • /
    • 1993
  • Rice (Oryza saliva L.) calli containing both embryogenic callus (EC) and non embryogenic callus (NEC) regions were initiated from the mature seed on MS medium supplemented with 2.0 mg/L 2,4-D, 0.5 mg/L kinetin. The calli were developed into two callus type which can be distinguished by visual examination depending on color and appearance. In order to illucidate the polypeptide composition between EC and NEC, the total protein extracted from two types of callus was analysed by electrophoresis. By one-dimesional anlaysis of SDS-PAGE and Isoelectric focusing, several protein bands showed quantitative and qualitative difference in each type of callus. The further analysis of the total protein with two-dimensional electrophoresis showed at least 20 EC specific protein and 10 NE specific protein. Also 3 specific protein spots showing micro heterogeneity of 90, 65, 50 kD were detected in EC, while a series of acidic heterologous protein spots were visualized in NEC.in NEC.

  • PDF

Organogenesis from Callus Derived from In Vitro Root Tissues of Wild Prunus yedoensis Matsumura

  • Cheong, Eun Ju
    • Journal of Forest and Environmental Science
    • /
    • v.35 no.1
    • /
    • pp.41-46
    • /
    • 2019
  • In vitro organogenesis system of the valuable ornamental species, Prunus yedoensis which is native to Korea, was established through callus culture derived from root tissues. Callus were induced on the medium supplemented with 2,4-D and BA or NAA and kinetin. Organogenesis was differ from the callus type, and NAA and kinetin combination was effective to induce organogenic callus. Growth of callus was influenced by sucrose concentrations. High level of sucrose (over 5%) had adverse effects such as decreased fresh weight and increased mortality of callus. Shoots developed from the callus when $GA_3$ was treated with BA in the medium. Results showed that $GA_3$ is essential for shoot development and elongation from callus in this species.

In vitro bioassay for allelopathic substances of Sorghum ( Sorghumbicolor L.) (수수로부터 allelopathy성 물질의 기내선별)

  • 유창연
    • Korean Journal of Plant Resources
    • /
    • v.7 no.2
    • /
    • pp.115-119
    • /
    • 1994
  • These experiments were conducted to determine the effects of Sorghum allelopathic substances on the callus growh of several weeds and crops. 1. When substances extracted from allelopathic Sorghum(Sorghum bicolor L.) were treated on medium, growth of callus of several weeds and crops were in-hibited. The degree of inhibition differed depending on the genotypes, ranging from 50 to 90% com-pared with that of control. 2. The extracts of above 5% Sorghum inhibited the callus growth of Che-nopodium albun L., Commelina communis L., and .Ammaranthus retroflexus L.and showed in-hibition rate of above 70% in callus growth. These results indicate that we could investigate theallelopaihy effect by using in vitro system. 3. The suitable explant for callus induction fromallelopathic plants was immature embryos, the callus induction rate differed depending on the geno-type, growth regulators and concentrations. In general, the addition of 2, 4-D and NAA onto medium increased the rate and amount of callus.

  • PDF

Production of Ginseng Saponins with Cell Culture(II) (세포배양에 의한 고려인삼 성분의 생산 연구(II))

  • Chi, Hyung-Joon;Shin, Kuk-Hyun;Kim, Hyun-Soo;Cho, Hee-Jae
    • Korean Journal of Pharmacognosy
    • /
    • v.20 no.3
    • /
    • pp.162-169
    • /
    • 1989
  • Tissue culture of the roots of Panax ginseng was carried out to enhance the production of ginseng callus as well as to increase its contents of ginsenosides. A long cylinder type callus mass was formed when cultured IK callus by rotary shaking culture method, the growth ratio of the callus being 7.71 which was approximately 4 fold higher than those obtained by other culture methods. Ginsenosides $Rg_1$, Re and $Rb_1$ could be detected from the callus mass by TLC, however, their total contents were revealed to be approximately 9% compared to that of the fresh ginseng root equivalent by HPLC analysis,.

  • PDF

Chromosome Variation in Callus Cells Derived from Different Cytogenetic Type Plants of Scilla scilloides Complex (세포유전적 유형이 다른 무릇(Scilla scilloidise Complex)에서 유도된 캘러스 세포의 염색체 변이)

  • Jae-Wook BANG;Jae-hyun PARK;Eun-Young Choi
    • Korean Journal of Plant Tissue Culture
    • /
    • v.21 no.1
    • /
    • pp.59-63
    • /
    • 1994
  • Chromosome variation in callus cells initiated from different cytogenetic type plane of Scilla scilloides Complex was analysed Considerable variation in both chromosome number and struchure was found in type AA, while no autosomal variation was detected in type BB callus cells. In allotetraploid AABB, two hypoploid cells were fount while a hypoploid cell and three hyperploid cells were found in eutetraploid cells of BBBB. Autosomes in callus cells derived from the plant with B-chromosomes were more stable than those from the plant without B-chromosome. We doubt that B-chromosomes have a selective function for the autosomes in culture of S. scilloides Complex.

  • PDF

In Vitro Propagation of Anthurium andreanum ′Atlanta′ Developed for Pot Culture (분화용 Anthurium andreanum ′Atlanta′의 기내번식)

  • Han, Bong-Hee;Goo, Dae-Hoe
    • Journal of Plant Biotechnology
    • /
    • v.30 no.2
    • /
    • pp.179-184
    • /
    • 2003
  • In order to establish micropropagation system Anthurium andreanum 'Atlanta', dwarf type, shoots of A. andreanum were cultured on medium supplemented with cytokinin. Callus was formed from the base of shoots. high frequency callus induction was obtained on medium with 10.0mg/L BA or 10.0mg/L TDZ(thidiazuron) at more than 71.8%. The shoots were cultured on media with various combinations and concentrations of TDZ, BA and 2.4-D to enhance callus induction. Callus was induced at more than 72.6% and grew vigorously on media containing 10.0mg/L BA and 0.0∼0.5mg/L 2.4-D, or 1.0mg/L TDZ. Stimulation effects of cytokinin by 2.4-D did not occur in combined treatments of cytokinin and 2.4-D. Callus was cut into sections(7${\times}$10mm), and then cultured on media with BA alone or BA and 2.4-D to regenerate shoots and to stimulate the callus growth. Shoot regeneration and callus growth were effective on media with 10.0mg/L BA alone, or 10.0mg/L BA and 0.1mg/L 2.4-D. In combined treatments of BA and 2.4-D, stmulation effects of cytocinin by 2.4-D also did not occur. Callus growth was decreased, accordiong to increasing the concentration of 2.4-D. In cimbined treatments of TDZ and 2.4-D in shoot regeneration and callus proliferation, stimulated effects of cytokinin by 2.4-D did not occur entirely. Media with 0.5∼1.0mg/L TDZ ingibited the regeneration and rooting of shoots, and callus growth from callus sections. Addition of 2.4-D on medium with TDZ ingibited the regeneration and rooting of shoots, and callus growth. Rooted plantdts were acclimatized in greenhouse. The plantlets were survived more than 98% in soil of vermiculite alone or mixed perlite 1 and vermiculite 1.