[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.7747/JFES.2019.35.1.41

Organogenesis from Callus Derived from In Vitro Root Tissues of Wild Prunus yedoensis Matsumura

Cheong, Eun Ju (Division of Forest Science, College of Forest and Environmental Science, Kangwon National University)

Publication Information

Journal of Forest and Environmental Science / v.35, no.1, 2019 , pp. 41-46 More about this Journal

Abstract

In vitro organogenesis system of the valuable ornamental species, Prunus yedoensis which is native to Korea, was established through callus culture derived from root tissues. Callus were induced on the medium supplemented with 2,4-D and BA or NAA and kinetin. Organogenesis was differ from the callus type, and NAA and kinetin combination was effective to induce organogenic callus. Growth of callus was influenced by sucrose concentrations. High level of sucrose (over 5%) had adverse effects such as decreased fresh weight and increased mortality of callus. Shoots developed from the callus when $GA_3$ was treated with BA in the medium. Results showed that $GA_3$ is essential for shoot development and elongation from callus in this species.

Keywords

Prunus yedoensis; callus culture; organogenesis; shoot development; root tissue;

Citations & Related Records

Reference

1	Brar J, Anand M, Sood A. 2015. Low sucrose and high gibberellic acid levels positively regulates relative growth rate and organogenesis in D. membranaceus Munro. Biochem Anal Biochem 4: 198.
2	Cheong EJ, Kim CS, Kinard G, Li R. 2015. Evaluation of the status of the virus and viroid infection in flowering cherry (Prunus yedoensis) collections in Korea and the U.S. J Plant Pathol 97: 155-160.
3	Cheong EJ, Kim CS, Yi JS. 2000. Differentiation of winter buds of Prunus yedoensis Matsumura from Jeju Island depending on the collection time and media conditions. J Korean Soc For Sci 89: 522-526.
4	Cheong EJ, Pooler MR. 2004. Factors affecting somatic embryogenesis in Prunus incisa cv. February Pink. Plant Cell Rep 22: 810-815. DOI
5	Criollo H, Perea M, Toribio M, Munoz J. 2014. Effect of the combination of NAA, kinetin and sucrose on the induction of somatic embryogenesis in lulo (Solanum quitoense Lam.). Agron Colomb 32: 170-179. DOI
6	Druart P. 1981. Somatic embryogenesis and plantlets obtained invitro from Prunus incisa serrula (GM 9). Bull Rech Agron Gembloux 16: 205-220.
7	Karami O, Deljou A, Esna-Ashari M, Ostad-Ahmadi P. 2006. Effect of sucrose concentrations on somatic embryogenesis in carnation (Dianthus caryophyllus L.). Sci Hortic 110: 340-344. DOI
8	Kim CS. 1998. Distribution and taxonomic study of Prunus yedoensis Matsumura (Rosaceae). MS thesis. Jeju National University, Jeju, Korea. (in Korean)
9	Kim YW, Park SY, Park IS, Moon HK. 2007. Somatic embryogenesis and plant regeneration from immature seeds of Magnolia obovata Thunberg. Plant Biotech Rep 1: 237-242. DOI
10	Lloyd G, McCown BH. 1980. Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. Comb Proc Int Plant Propag Soc 30: 421-427.
11	Murashige T, Skoog F. 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15: 473-497. DOI
12	Salaj T, Matusova R, Salaj J. 2015. Conifer somatic embryogenesis-an efficient plant regeneration system for theoretical studies and mass propagation. Dendrobiology 74: 69-76. DOI
13	Takashina T, Nakano H, Kato R. 2003. Efficient plant regeneration culture from leaf explants of in vitro-grown sweet cherry. Acta Hortic 622: 123-127. DOI
14	Lipavska H, Konradova H. 2004. Somatic embryogenesis in conifers: The role of carbohydrate metabolism. In Vitro Cell Dev Biol Plant 40: 23-30. DOI
15	Koh JK, Park YC, Yang DY, Kim ES, Oh MY, Koh SC. 1997. Plant regeneration and somatic embryogenesis from zygotic embryo-derived callus of native Prunus yedoensis in Mt. Halla. Korean J Plant Tiss Cult 24: 345-349.
16	Korea Forest Service. 2012. Reports on landscape species planting in Korea in 2012. Korea Forest Service, Urban Forest and Landscape Division, Daejeon. (in Korean)
17	Lardet L, Dessailly F, Carron MP, Montoro P, Monteuuis O. 2009. Influences of aging and cloning methods on the capacity for somatic embryogenesis of a mature Hevea brasiliensis genotype. Tree Physiol 29: 291-298. DOI
18	Compton ME, Gray DJ. 1996. Effects of sucrose and methylglyoxal bis-(guanylhydrazone) on controlling grape somatic embryogenesis. Vitis 35: 1-6.
19	Vondrakova Z, Eliašova K, Fischerova L, Vagner M. 2011. The role of auxins in somatic embryogenesis of Abies alba. Cent Eur J Biol 6: 587-596. DOI
20	Tsukamoto T, Gao M, Negoro K, Hanada H, Tao R, Kawabe M, Yonemori K. 2007. Somatic embryogenesis and plant regeneration from immature cotyledons of Prunus mume 'Nanko'. Acta Hortic 738: 697-701. DOI
21	Wang PJ, Hu CY. 1980. Regeneration of virus-free plants through in vitro culture. In: Advances in Biomedical Engineering, Volume 18 (Fiechter A, ed). Springer, Berlin, Heidelberg.
22	Bhagwat B, Lane WD. 2004. In vitro shoot regeneration from leaves of sweet cherry (Prunus avium) 'Lapins' and 'Sweetheart'. Plant Cell Tiss Org Cult 78: 173-181. DOI
23	Ainsley PJ, Collins GG, Sedgley M. 2000. Adventitious shoot regeneration from leaf explants of almond (Prunus dulcis mill.). In Vitro Cell Dev Biol Plant 36: 470-474. DOI