• Journal of Periodontal and Implant Science
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• v.34 no.1
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• pp.113-126
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• 2004

연구목적 : 이번 실험의 목적은 외과적으로 형성된 성견의 1면 골내낭에 새롭게 제조된, Ca/P 비율이 0.6인 비결정성 calcium phosphate를 적용하였을 때 치주조직의 재생에 미치는 영향을 평가하는 것이다. 연구방법 : 6마리 성견의 양측 하악 제2소구치의 원심면, 제4소구치의 근섬면에 외과적으로 1면 골내낭을 형성하여 치은박리소파술을 시행한 부위를 대조군으로, calcium phosphate만을 이식한 부위를 실험 1군, calcium phosphate와 GTR을 동반한 부위를 실험 2군으로 설정하고 실험하여 술 후 8주에 치유결과를 조직학적으로 관찰하였다. 결론 : 1. 접합상피의 치근단 이동은 대조군에서 결손부 깊이의 30.90 ${\pm}$ 9.92%, 실험 1군에서 결손부 깊이의 24.08 ${\pm}$ 9.12%, 실험 2군에서 결손부 깊이의 38.68 ${\pm}$ 12.22%로 나타났으며 대조군과 실험 1,2군간에 통계적 유의차는 없었다. 2. 결합조직 유착은 대조군에서 결손부 깊이의 36.38 ${\pm}$ 9.03%, 실험 1군에서 결손부 깊이의 26.96 ${\pm}$ 4.24%, 실험 2군에서 결손부 깊이의 27.87 ${\pm}$ 9.70%로 나타났으며 대조군과 실험 1,2군간에 통계적 유의차는 없었다. 3. 신생백악질 형성은 대조군에서 결손부 깊이의 32.92 ${\pm}$ 10.51%, 실험 1군에서 결손부 깊이의 49.16 ${\pm}$ 12.70%, 실험 2군에서 결손부 깊이의 39.62 ${\pm}$ 12.14%로 나타났으며 대조군과 실험 1군간에 통계적 유의차가 있었다. 4. 신생골 형성은 대조군에서 결손부 깊이의 27.24 ${\pm}$ 7.49%, 실험 1군에서 결손부 깊이의 43.51 ${\pm}$ 13.34%, 실험 2군에서 결손부 깊이의 36.47 ${\pm}$ 15.11%로 나타났으며 대조군과 실험 1, 2군간에 통계적 유의차는 없었다. 이상의 결과에서 볼 때, calcium phosphate glasses는 신생골 형성에는 통계적으로 유의차는 없었지만 상당히 증가된 양상을 보였고 신생백악질 형성에는 크게 기여함을 알수 있었다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.37 no.5
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• pp.415-420
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• 2011

Purpose: Calcium phosphate cement (CPC) is one of many useful materials for restoring tooth defects, periodontium and maxillofacial area. Chitosan is a biodegradable material that has been shown to promote the growth and differentiation of osteoblasts in culture. This study examined the interaction between odontoblasts and bio-calcium phosphate cement reinforced with chitosan. Materials and Methods: $5{\times}10^3$ odontoblastic cells were seeded into each well. Various concentrations of bio-calcium phosphate cement reinforced with chitosan (10, 20, 50, 100, 200, 500 ${\mu}g$/ml, 1, 2, 4 mg/ml) were diluted and added to the wells. The well was incubated for 24 h, 48 h and 72 h. After incubation, the number of cells was assessed to determine the cell viability. A cytokinesis-block micronucleus assay and chromosomal aberration test were carried out to estimate the extent of chromosomal abnormalities. Microscopic photographs and RT-PCR were performed to examine the adhesion potential of bio-calcium phosphate cement reinforced with chitosan. Results: Bio-CPC-reinforced chitosan did not show significant cytotoxicity. The number of damaged chromosomes in the cells treated with Bio-CPC-reinforced chitosan was similar to that in the control cells. There was no significant increase in the number of chromosomal aberrations in the Bio-CPC reinforced chitosan exposed cells. Microscopic photographs and RT-PCR confirmed the adhesive potential of bio-CPC reinforced chitosan to odontoblasts. Conclusion: Bio-CPC-reinforced chitosan did not affect the odontoblastic cell viability, and had no significant cytotoxic effect. Bio-CPC-reinforced chitosan showed adhesive potential to odontoblasts. These results are expected form the basis of future studies on the effectiveness of dental restorative materials in Bio-CPC reinforced with chitosan.

• Proceedings of the Materials Research Society of Korea Conference
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• 2009.11a
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• pp.42.1-42.1
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• 2009

In recent years, it has been tried to develop the efficacy and bioactivity of Calcium Phosphate cements(CPC) as injectable bone substitute (IBS) by reinforcing them through varying the amount in its compositions and relative concentrations or adding other additives. In this study, the biocompatibility of are inforced Calcium Phosphate-Calcium Sulfate injectable bone substitute (IBS)containing poly ($\varepsilon$-caprolactone)PCL microspheres was evaluated which consisted of solution chitosan and Na-citrate as liquid phase and tetra calcium phosphate (TTCP), dicalciumphosphate anhydrous (DCPA) powder as the solid phase. The in vitrobiocompatibility of the IBS was done using MTT assay and Cellular adhesion and spreading studies. The in vitro experiments with simulated body fluid (SBF) confirmed the formation of apatite on sample surface after 7 and 14 days of incubation in SBF. SEM images for one cell morphologies showed that the cellular attachment was good. MG-63 cells were found to maintain their phenotype on samples and SEM micrograph confirmed that cellular attachment was well. In vitro cytotoxicity tests by an extract dilution method showed that the IBS was cytocompatible for fibroblast L-929.

• Proceedings of the KACD Conference
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• 2001.11a
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• pp.567.1-567
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• 2001

A self-setting calcium phosphate cement (CPC), consisting of tetracalcium phosphate (TTCP) and dicalcium phosphate anhydrous (DCP A), reacts with water and hardens fast (30 min) to form hydroxyapatite (HA) under physiological conditions as the final product. Although this CPC is finding increasing use as a biomaterial, it is presently limited to low stress bearing applications because of its relatively low strength and highly brittle nature. Recently the mechanical properties of CPC reinforced with chopped carbon fiber have been reported.

• Proceedings of the KACD Conference
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• 2001.11a
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• pp.566.1-566
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• 2001

The most desirable healing process for endodontic therapy is apical closure by hard tissue such as dentine or cementum. Then, we estimated hard tissue conductivity of tetracalcium phosphate (4CP)/dicalcium phosphate (2CP) compound using mandibular first molars of SD rats. Residual pulp responses to the calcium phosphate compound were examined at several amputation levels of pulp. 2CP was purchased and passed through a $32-\mu\textrm{m}$ sieve. 4CP was obtained from a stoichiometric mixture of 2CP and calcium carbonate (Mol ratio: Ca/P=2.0) by the dry synthetic method at 1, 400(C for 8 hours.(omitted)

• Journal of Life Science
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• v.23 no.2
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• pp.242-248
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• 2013

The objective of this study was to develop a mineral phosphate-solubilizing bacterium as a biofertilizer. A mineral phosphate-solubilizing bacterium HR-1019 was isolated from cultivated soils. It was identified as Bacillus subtilis by 16S rDNA analysis. The phosphate-solubilizing activities of the HR-1019 strain against three types of insoluble phosphate, hydroxyapatite, tri-calcium phosphate, and aluminum phosphate were quantitatively determined. When 5% of glucose concentration was used as a carbon source, the strain showed marked mineral phosphate-solubilizing activity. Mineral phosphate solubilization was directly related to pH drop in the culture solution of the strain. The pathogenic activity and antifungal effects of the HR-1019 strain were measured inclear zones formed in PDA media.

• Journal of Dental Rehabilitation and Applied Science
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• v.24 no.3
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• pp.311-316
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• 2008

Calcium phosphate cement (CPC) has been used as bone substitute successfully due to good biocompatibility and osteoconductivity. One of the important mechanical characteristics of CPC is flowablility, which can be evaluated by measuring rheological parameters. However, there have been few studies that measured rheological properties of CPC. The purpose of this study was to evaluate the rheological properties of CPC paste mixed with 2 kinds of setting solutions, 2% hydroxyprophyl methylcellulose (HPMC) and 35% polyacrylic acid (PAA). The CPC used was dicalcium phosphate dihydrate (DCPD). Rheological properties of CPC paste were measured using rheometer. The statistical analysis was carried out with Mann-whitney test with Bonferronis collection. CPC with both setting solutions showed shear thinning behavior. CPC with 2% HPMC showed signigicantly higher complex viscosity than CPC with 35% PAA(p<0.05).

• KSBB Journal
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• v.13 no.5
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• pp.497-501
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• 1998

A fungus with high phosphate solubilizing activites was isolated from soil using potato dextrose agar-calcium phosphate medium and identified to Penicillium sp. PS-113, based on the morphological characteristics of conidiophore and conidia; flask shape of phialide, simple branching type of conidiophore, and columnar shape of conidial head, in malt extract agar and potato dextrose agar media. The optimum temperature ad initial pH to solubilize rock phosphate in potato dextrose broth-rock phosphate medium were 30$^{\circ}C$ and pH 8.0, respectively. In these conditions phosphate solubilizing activities of Penicillium sp. PS-113 against four types of insoluble phosphate like tricalcium-phosphate, aluminium phosphate, hydroxyapatite and rock phosphate were quantitatively determined. As results, this fungus highly produced free phosphates to the culture broth with the concentrations of 1,283 ppm against tricalcium-phosphate, 585 ppm against rock phosphate, 528 ppm against aluminium phosphate, and 242 ppm against hydroxyapatite, respectively.

• Journal of the Korean Ceramic Society
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• v.26 no.1
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• pp.67-72
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• 1989

In order to define the composition range of bone china body with use of calcium phosphate, kaolin, quartz and feldspar as raw materials, we varied calcium phosphate from 40 to 55wt/o and selected basic composition by the change of raw materials in ternary system. After these bodies were fired at 120$0^{\circ}C$, 123$0^{\circ}C$, 125$0^{\circ}C$ and 128$0^{\circ}C$ their properties were closely tested to determine the compositions and firing conditions of bone china body.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2006.04a
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• pp.428-431
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• 2006

오염된 토양이나 지하수에 존재하는 중금속을 고정화시키기 위한 소스로 세 가지 종류의 뼈를 검토해보았다. 뼈 속에 있는 여러 종류의 calcium phosphate를 XPS로 분석한 결과, $Ca_8H_2(PO_4)_6.5H_2O$(octa-Calcium Phosphate)가 공통적으로 들어 있었고, 참치 뼈에는 유일하게 유기물 형태의 인이 들어 있었다. 증류수를 이용한 실험에서 뼈 속의 인산염은 액상에 쉽게 용출되기 때문에 중금속 고정화에 중요한 소스가 될 수 있다는 것을 알았다.