Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Isolation and Characterization of a Novel Bacterium, Bacillus subtilis HR-1019, with Insoluble Phosphates Solubilizing Activity

인산가용화 활성을 갖는 바실러스 서브틸리스 HR-1019 분리와 특성

  • Lee, Yong-Suk (Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University) ;
  • Park, Dong-Ju (Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University) ;
  • Kim, Jae Hoon (Haerim Pharmetic LTD.) ;
  • Kim, Hyeong Seok (Haerim Pharmetic LTD.) ;
  • Choi, Yong-Lark (Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University)
  • Received : 2012.11.29
  • Accepted : 2013.01.25
  • Published : 2013.02.28
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Abstract

The objective of this study was to develop a mineral phosphate-solubilizing bacterium as a biofertilizer. A mineral phosphate-solubilizing bacterium HR-1019 was isolated from cultivated soils. It was identified as Bacillus subtilis by 16S rDNA analysis. The phosphate-solubilizing activities of the HR-1019 strain against three types of insoluble phosphate, hydroxyapatite, tri-calcium phosphate, and aluminum phosphate were quantitatively determined. When 5% of glucose concentration was used as a carbon source, the strain showed marked mineral phosphate-solubilizing activity. Mineral phosphate solubilization was directly related to pH drop in the culture solution of the strain. The pathogenic activity and antifungal effects of the HR-1019 strain were measured inclear zones formed in PDA media.

본 연구의 목적은 우수한 미생물 제제 개발용 인산염 가용화 균주의 개발이다. 경작지 토양에서 분리한 인산염 가용화 균주의 특성 및 16S rDNA 염기서열을 조사한 결과 Bacillus subtilis HR-1019로 동정되었다. Bacillus subtilis HR-1019는 hydroxyapatite, tri-calcium phosphate 및 aluminum phosphate 3가지의 난용성 인산염을 모두 가용화하였다. 난용성 인산염의 분해능이 최대가 되는 배양온도는 $37^{\circ}C$이었으며, 배양초기 pH가 5.0이었다. 탄소원으로 glucose를 5% 첨가시 가용화능이 높았으며, 가용화된 유리인산의 함량이 증가함에 따라 pH가 크게 감소하였다. 분리균주 HR-1019가 식물병원균에 대하여 균의 생육을 저지하는 clear zone 확인으로 항균효과를 확인하였다.

Keywords

References

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