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http://dx.doi.org/10.5352/JLS.2013.23.2.242

Isolation and Characterization of a Novel Bacterium, Bacillus subtilis HR-1019, with Insoluble Phosphates Solubilizing Activity  

Lee, Yong-Suk (Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University)
Park, Dong-Ju (Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University)
Kim, Jae Hoon (Haerim Pharmetic LTD.)
Kim, Hyeong Seok (Haerim Pharmetic LTD.)
Choi, Yong-Lark (Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University)
Publication Information
Journal of Life Science / v.23, no.2, 2013 , pp. 242-248 More about this Journal
Abstract
The objective of this study was to develop a mineral phosphate-solubilizing bacterium as a biofertilizer. A mineral phosphate-solubilizing bacterium HR-1019 was isolated from cultivated soils. It was identified as Bacillus subtilis by 16S rDNA analysis. The phosphate-solubilizing activities of the HR-1019 strain against three types of insoluble phosphate, hydroxyapatite, tri-calcium phosphate, and aluminum phosphate were quantitatively determined. When 5% of glucose concentration was used as a carbon source, the strain showed marked mineral phosphate-solubilizing activity. Mineral phosphate solubilization was directly related to pH drop in the culture solution of the strain. The pathogenic activity and antifungal effects of the HR-1019 strain were measured inclear zones formed in PDA media.
Keywords
Insoluble phosphate; mineral phosphate-solubilization; free phosphate; Bacillus subtilis HR-1019; plant pathogen;
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