• 제목/요약/키워드: calcium concentration

검색결과 1,651건 처리시간 0.032초

Redistribution of Intracellular Calcium Stores with Shear Stress-induced Cytoskeleton Organization in Human Endothelial Cell

  • 정찬일;장현아;장준근;한동철;민병구
    • 대한의용생체공학회:학술대회논문집
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    • 대한의용생체공학회 1996년도 춘계학술대회
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    • pp.97-99
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    • 1996
  • Intracellular gradients of the free calcium concentration are thought to be critical for the localization of functional responses within a cell. The mechanism of mechanotransduction may be associated with the localized accumulation of calcium stores for shear stress-exposed endothelial cells. The distribution of the calcium stores and the formation of the stress fibers were investigated by the indirect double immunofluorescent staining method with the calreticulin antibody and rhodamine phalloidin under flow condition. The shear stress of steady flow reorganized the cytoskeleton structure including the bundling and translocation to focal contacts. The calcium stores translocated from the cytoplasm to the focal contacting area. Consequently. accumulation of the calcium stores may participate in the shear stress-induced cytoskeleton organization of endothelial cells.

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소 혈청 알부민이 함유된 유사체액 내에서 아파타이트의 생성에 대한 고분해능 전자현미경 분석 (HREM Analysis of Apatite Formation in Modified-Simulated Body Fluid Containing Bovine Serum Albumin)

  • 김우정;이갑호;홍순익
    • 대한금속재료학회지
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    • 제46권2호
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    • pp.105-110
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    • 2008
  • Process of the hydroxyapatite (HA) formation on bioactive titanium metal prepared by NaOH treatment in a modified-simulated body fluid (mSBF) containing bovine serum albumin (BSA) was investigated by high resolution transmission electron microscope attached with energy dispersive X-ray spectrometer (EDX). The amorphous titanate, which was formed on titanium surface by NaOH treatment, combined with the calcium ions in the liquid to form an amorphous calcium titanite. With increasing of soaking time in the liquid, an amorphous calcium titanite combined with the phosphate ions to form an amorphous calcium phosphate with low Ca/P atomic ratio, and it grows as aggregates of plate (or needle)-like substance on titanium surface. The crystalline apatite layers, which are needle-shaped with the c axis parallel to the long axis, are formed in an amorphous calcium phosphate with further increase in soaking time. The formation of needle-shaped apatite layers can be explained by electrostatic effects and difference of concentration between calcium, phosphate, and albumin ions.

치오글리콜산(酸)칼슘의 안정성(安定性) (Decomposition Rate of Calcium Thioglycollate)

  • 이계주;유병태
    • Journal of Pharmaceutical Investigation
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    • 제11권3호
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    • pp.21-26
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    • 1981
  • The rate of decomposition of calcium thioglycollate in aqueous solution was shown to increase with increasing temperature and with diluting concentration of calcium thioglycollate. The effect of additive on oxidation rate of calcium thioglycollate was studied to prepare the more stable depilatory formula using additives, such as thiourea, mannitol, sodium thiocyanide, nicotinamide, triethanolamine and ethylenediamine. In all cases, the rate of oxidation was reduced when the additive was present in the solution, among them triethanolamine and ethylenediamine inhibited the decomposition rate remarkably. It is assumed that the decomposition reaction in aqueous solution is pseudo-first order reaction.

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Evaluation of Salt, Microbial Transglutaminase and Calcium Alginate on Protein Solubility and Gel Characteristics of Porcine Myofibrillar Protein

  • Hong, Geun-Pyo;Chin, Koo-Bok
    • 한국축산식품학회지
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    • 제30권5호
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    • pp.746-754
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    • 2010
  • Response surface methodology was adopted to model and optimize the effects of microbial transglutaminase (TG) and calcium alginate (CA) systems of various ratios on the gelation characteristics of porcine myofibrillar protein (MP) at various salt levels. The CA system consisting of sodium alginate (SA), calcium carbonate (CC) and glucono-$\delta$-lactone (GdL) showed no remarkable changes in the salt-soluble fraction, and only minor effects on electrostatic interactions were observed. Increasing CA concentration caused acid-induced hydrophobic interactions in MPs, resulting in increased MP gel strength. The TG system, containing TG and sodium caseinate (SC), induced cold-set MP gelation by formation of covalent bonding. The main advantage of the combined system was a higher cooking yield when the MP gel was heated. These results indicated that 0.7% TG combined with 0.8% CA system can form a viscoelastic MP gel, regardless of salt levels.

Ca(OH)2촉매를 이용한 플라즈마 반응에 의한 황산화물의 제거에 관한 연구 (A study of decomposition of sulfur oxides using Calcium hydroxide catalyst by plasma reactions)

  • 김다영;우인성;이선희;김도현;김병철
    • 대한안전경영과학회:학술대회논문집
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    • 대한안전경영과학회 2013년 추계학술대회
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    • pp.547-560
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    • 2013
  • In this study, the air pollutant removal such as sulfur oxides was studied. A combination of the plasma discharge in the reactor by the reaction surface discharge reactor Calcium hydroxides catalytic reactor and air pollutants, hazardous gas SOx, changes in gas concentration, change in frequency, the thickness of the electrode, kinds of electrodes and the addition of simulated composite catalyst composed of a variety of gases, including decomposition experiments were performed by varying the process parameters. The experimental results showed the removal efficiency of 98% in the decomposition of sulfur oxides removal experiment when Calcium hydroxides catalysts and the tungsten(W) electrodes were used. It was increased 3% more than if you do not have the catalytic. If added to methane gas was added the removal efficiency increased decomposition.

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Manufacture and Properties of Inorganic Chemical Treated Wood by Introducing of Fluorides

  • Kim, Soung-Joon;Lee, Jong-Shin
    • Journal of the Korean Wood Science and Technology
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    • 제36권2호
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    • pp.73-78
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    • 2008
  • Inorganic chemical treated wood was prepared by impregnation of calcium or magnesium chloride ($CaCl_2$ or $MgCl_2$) solution and immersion in saturated solution of ammonium fluoride ($NH_4F$) as a reactant in order to make an introduction of a refractory fluorides with fungicidal and insecticidal effects in wood. The weight percent gains (WPGs) were increased with increase in concentration of calcium chloride or magnesium chloride solution, and were higher in treatment with calcium chloride than with magnesium chloride. Inorganic substances were produced mainly in the lumina of tracheides. These substances were proved to be the calcium fluoride or magnesium fluoride by the energy dispersive X-ray analyzer in conjunction with a scanning electron microscope (SEM-EDX). The treated wood showed good decay resistance because the weight losses were hardly occurred by the test fungi such as Tyromyces palustris and Trametes versicolor. The fire resistance effect was superior to the treated wood compared with that of the untreated wood.

생쥐 대식세포의 활성시 신호전달에 미치는 인삼성분들의 영향 (The Effects of Ginseng Components on the Signal Transduction in the Activation of Murine Macrophages)

  • 신은경;박한우
    • Journal of Ginseng Research
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    • 제20권2호
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    • pp.159-167
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    • 1996
  • To study the effects of ginseng saponin components on the signal transduction in the ac tivation of murine macrophages, phagocytosis and Intracellular calcium concentration of peritoneal exuded mouse macrophages were examined. The phagocytosis was increased significantly after treatment with total saponin, diol-saponin, $Rg_1$ and $Rg_2$, but triol-saponin was unable to increase phagocytosis. The phagocytosis were increased when H7, a PKC inhibitor, was pretreated and increased significantly by saponin fractions except total saponin. Pertussis toxin, which inactivates G-protein, decreased the phagocytosis. But the phagocytosis was restored to the control level by saponin fractions and the phagocytosis was increased significantly by $Rg_2$ and $Rg_2$. The triol saponin increased phagocytosis approximately by 2-fold as compared with the TMB-8 treated group. Peritoneal exuded macrophages displayed a prominent rise in cytosolic calcium following treatment with triol-saponin, $Rg_1$, $Rg_2$ and $Rg_2$. Incubation of macrophages with PT resulted in an inhibition of cytosolic calcium mobilization, but increased cytosolic calcium mobilization with saponin fraction.

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건조새우를 이용한 칼슘강화 건강다과 개발 (Development of Calcium Enriched Healthy Snack using Dried Shrimp)

  • 박은빈;유수인;김연재;백진경
    • 한국식품영양학회지
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    • 제35권1호
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    • pp.1-6
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    • 2022
  • In this study, we developed a healthy snack using dried shrimp powder which is rich in calcium to help prevent bone disease. Different types of yanggaeng were prepared with varying ratios of dried shrimp powder; 1%, 3%, 5%, and 7%. Moisture content, color value, texture properties, and mineral concentration were measured. The L, a, and b chromaticity values showed significant differences in the 5% addition group (p<0.001). The texture properties, hardness, and adhesion decreased significantly as the amount of shrimp powder increased (p<0.001), while chewiness and elasticity significantly increased as the amount increased (p<0.001). Calcium and potassium increased significantly as the amount of shrimp powder increased (p<0.001).

Evaluation of Acid Digestion Procedures to Estimate Mineral Contents in Materials from Animal Trials

  • Palma, M.N.N.;Rocha, G.C.;Valadares Filho, S.C.;Detmann, E.
    • Asian-Australasian Journal of Animal Sciences
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    • 제28권11호
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    • pp.1624-1628
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    • 2015
  • Rigorously standardized laboratory protocols are essential for meaningful comparison of data from multiple sites. Considering that interactions of minerals with organic matrices may vary depending on the material nature, there could be peculiar demands for each material with respect to digestion procedure. Acid digestion procedures were evaluated using different nitric to perchloric acid ratios and one- or two-step digestion to estimate the concentration of calcium, phosphorus, magnesium, and zinc in samples of carcass, bone, excreta, concentrate, forage, and feces. Six procedures were evaluated: ratio of nitric to perchloric acid at 2:1, 3:1, and 4:1 v/v in a one- or two-step digestion. There were no direct or interaction effects (p>0.01) of nitric to perchloric acid ratio or number of digestion steps on magnesium and zinc contents. Calcium and phosphorus contents presented a significant (p<0.01) interaction between sample type and nitric to perchloric acid ratio. Digestion solution of 2:1 v/v provided greater (p<0.01) recovery of calcium and phosphorus from bone samples than 3:1 and 4:1 v/v ratio. Different acid ratios did not affect (p>0.01) calcium or phosphorus contents in carcass, excreta, concentrate, forage, and feces. Number of digestion steps did not affect mineral content (p>0.01). Estimated concentration of calcium, phosphorus, magnesium, and zinc in carcass, excreta, concentrated, forage, and feces samples can be performed using digestion solution of nitric to perchloric acid 4:1 v/v in a one-step digestion. However, samples of bones demand a stronger digestion solution to analyze the mineral contents, which is represented by an increased proportion of perchloric acid, being recommended a digestion solution of nitric to perchloric acid 2:1 v/v in a one-step digestion.

Identification of Lys49-PLA2 from crude venom of Crotalus atrox as a human neutrophil-calcium modulating protein

  • Sultan, Md. Tipu;Li, Hong-Mei;Lee, Yong Zu;Lim, Soon Sung;Song, Dong-Keun
    • The Korean Journal of Physiology and Pharmacology
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    • 제20권2호
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    • pp.177-183
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    • 2016
  • We fortuitously observed a human neutrophil intracellular free-calcium concentration ($[Ca^{2+}]_i$) increasing activity in the commercially available phosphodiesterase I (PDE I), which is actually dried crude venom of Crotalus atrox. As this activity was not observed with another commercially available pure PDE I, we tried to find out the causative molecule(s) present in 'crude' PDE, and identified Lys49-phospholipase A2 (Lys49-PLA2 or K49-PLA2), a catalytically inactive protein which belongs to the phospholipase A2 family, by activity-driven three HPLC (reverse phase, size exclusion, reverse phase) steps followed by SDS-PAGE and LC-MS/MS. K49-PLA2 induced $Ca^{2+}$ influx in human neutrophils without any cytotoxic effect. Two calcium channel inhibitors, 2-aminoetoxydiphenyl borate (2-APB) ($30{\mu}M$) and SKF-96365 ($20{\mu}M$) significantly inhibited K49-PLA2-induced $[Ca^{2+}]_i$ increase. These results suggest that K49-PLA2 modulates $[Ca^{2+}]_i$ in human neutrophils via 2-APB- and SKF-96365-sensitive calcium channels without causing membrane disruption.