• Journal of Microbiology and Biotechnology
• /
• v.9 no.4
• /
• pp.475-481
• /
• 1999

The study of lignin, a major component of secondary cell wall, has been partly focused on its removal from the woody part in the kraft pulping industry. Cinnamyl alcohol dehydrogenase (CAD; EC 1.1.l95) catalyzes the synthesis of cinnamyl alcohols from corresponding cinnamaldehydes. A cDNA clone, MdCADl, encoding putative CAD from apples (Malus domestica Borkh. cv Fuji) was characterized in this study. The clone contains an open reading frame of 325 amino acid residues, which shows a greater than 80％ identity with Eucalyptus CADl. MdCADl mRNA was detectable in vegetative tissues and was strongly expressed in the fruit. The expression pattern of MdCADl mRNA in the fruit peel after light exposure was also examined. The mRNA was rapidly increased until 1 day after light exposure and remained stable thereafter, suggesting that MdCADl is light inducible. The inducibility of the MdCADl gene was examined using several environmental stresses. Mechanical wounding of leaves increased the MdCADl mRNA level and the induction was further increased by salicylic acid. Southern blot hybridization showed that there is either one or a few copies of CAD genes in apples. To our knowledge, it is believed that MdCADl is the first CAD clone expressed predominantly in fruit.

• Korean Journal of Medicinal Crop Science
• /
• v.17 no.4
• /
• pp.266-272
• /
• 2009

Cinnamyl alcohol dehydrogenase (CAD, EC 1.1.1.95), catalyzes the reduction of hydroxycinnamaldehydes to give hydroxycinnamyl alcohols, or "monolignols," the monomeric precursors of lignin. Lignins are important components of cell walls and lignified secondary cell walls play crucial roles in long distance transport of water and nutrients during plant growth and development and in plant defense against biotic and abiotic stresses. Here a cDNA clone containing a CAD gene, named as PgCAD, was isolated from a commercial medicinal plant Panax ginseng. PgCAD is predicted to encode a precursor protein of 177 amino acid residues, and its sequence shares high homology with a number of other plant CADS. The expression of PgCAD in adventitious roots and hairy roots of P. ginseng was analyzed using reverse transcriptase (RT)-PCR under various abiotic stresses such as salt, salicylic acid, wounding and chilling treatment that triggered a significant induction of PgCAD at different time points within 2-48 h post-treatment. This study revealed that PgCAD may help the plants to survive against various abiotic stresses.

• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 1997.04a
• /
• pp.724-728
• /
• 1997

A CAD system of grid element for vacuum fluorescent disply has been developed. In order to reduce design man-houre and human erros, it is used to automate the design process using a knowledge base system. In the case of VFD product design, the most important consideration is the short-life cycle. So the development of CAD system for VFD product is needed. The developed system is based on the knowledge base system which is involved in a lot of expert's technology in the practice field. Using C-language under the HP-UNIX system, CIS customer language of the EXCESS CAD/CAM is used as the overall CAD environment. Results of this system will provide effective aids to the designer in this field

• Journal of Korean Institute of Industrial Engineers
• /
• v.17 no.2
• /
• pp.1-16
• /
• 1991

This paper deals with some critical activities of CAPP system such as generation of part description database, part feature recognition, process and operation selection, and sequencing method for turning operation of symmetric rotational parts. The part description database is generated by data conversion module from CAD data, and the part feature is recognized by using both pattern primitives and feature recognition rules. Machining processes and operations are selected based on machining surface features and its sequence is determined by rules acquired from process planning expert. AutoCAD is employed as CAD system and computer program is developed by using Turbo-C on IBM PC/AT compatible system.

• Journal of the Korean Society of Safety
• /
• v.8 no.4
• /
• pp.54-60
• /
• 1993

The purpose of this study is to develop a CAD/CAM system for automatic creation and manufacturing of three dimension objects. The computer system used in this study made RAM 2M, CPU 80386, VGA graphic card. The results in this paper are as follows ; 1. By interconnect PC(personal computer) and NC(numerical control) milling machine with RS232C connector, we was constructed with CAD/CAM system. 2. The developed algorithm in this study is able to be design of three dimension object on the computer CRT and manufacturing of NC milling machine. 3. Because of design and modifying on the PC of objects, we can be saving time, cost and improvable precision of objects. 4. Essentially, we expect industrial accident to reduse according as we takes advantage of CAD/CAM system.

• Proceedings of the KIEE Conference
• /
• 2001.10a
• /
• pp.30-32
• /
• 2001

There are many difficult problems in analyzing the flow characteristics in a high voltage circuit breaker such as shock wave and complex geometries, which may be either static or in relative motion. Although a variety of mesh generation techniques are now available, the generation of meshes around complicated, multi-component geometries like a gas circuit breaker is still a tedious and difficult task for the computational fluid dynamics. This paper presents the CFD program for analyzing the compressible flow fields in a high voltage gas circuit breaker using the Cartesian cut-cell method based on the CFD-CAD integration, which can achieve the accurate representation of the geometry designed by a CAD tools. This technique is frequently satisfied, and it will be almost universally so in the future, as the CFD-CAD traffic increase.

• International conference on construction engineering and project management
• /
• 2005.10a
• /
• pp.515-519
• /
• 2005

Most information within a construction project roots in drawings and contract documents. Although several methodologies, such as A/E/C and 4D, have employed such a peculiarity to integrate project information, they failed to integrate information in terms of efficiency and consistency. In this research, a MD (Multi-Dimensional) CAD model is developed to improve the process of integrating information for construction projects. This research describes the processes of creating MD CAD objects and embedding information required throughout the project life cycle within MD CAD building components. In addition, a case study is presented to show the efficiency and effectiveness of using MDIIS (Multi-Dimensional Information Integration System) to integrate information for construction projects.

• Korean Journal of Computational Design and Engineering
• /
• v.9 no.4
• /
• pp.373-381
• /
• 2004

Using standard components of a press die is recognized as a way for a cost reduction and a short lead time. It also provides a way for a quick maintenance of a die under repair. It is expected to contribute to integration of CAD/CAM system for manufacturing dies in the future. This paper presents a 3D CAD library which is constructed using the standard components and is used for designing a press die. This 3D CAD library is generated by a database system made of Microsoft Access for standard components and by CATIA V5 R10 API for geometric features. The library is implemented using Visual Basic 6.0 utility of CATIA API function in the Windows NT environment. It creates a 3D model of the standard components of press die easily when a die designer inputs numerical values of geometric features and the BOM of the completely assembled parts. It also generates automatically the assembly drawing of die set by using variables for standard values of die parts. Therefore users can save the cost of time to design the press die components, and even a beginner can use this program with ease. The test results of the 3D CAD library for designing shearing and bending dies verify its usefulness and feasibility.

• Proceedings of the KSME Conference
• /
• 2001.11b
• /
• pp.523-528
• /
• 2001

There are many difficult problems in analyzing the flow characteristics in a high voltage circuit breaker such as shock wave and complex geometries, which may be either static or in relative motion. Although a variety of mesh generation techniques are now available, the generation of meshes around complicated, multicomponent geometries like a gas circuit breaker is still a tedious and difficult task for the computational fluid dynamics. This paper presents the computational method for analyzing the compressible flow fields in a high voltage gas circuit breaker using the Cartesian cut-cell method based on the CFD-CAD integration, which can achieve the accurate representation of the geometry designed by a CAD tools. The technique is frequently satisfied, and it will be almost universally so in the future, as the CFD-CAD traffic increases.

• International Journal of Precision Engineering and Manufacturing
• /
• v.6 no.4
• /
• pp.8-13
• /
• 2005

Interoperability has been the problem of CAD/CAM systems. Starting from 1980's, national and international organizations have addressed the issue through development and release of standards for the exchange of geometric and nongeometric design data. To CAD/CAM vendors, the task of interpreting and implementing these standards falls into their products. This task is a balancing action between users' needs, available development resources, and the technical specifications of standards. This paper explores an area of CAD/CAM systems development, particularly the implementation of the effective exchange files translators'. A new approach is introduced, which proposes to enclose all the translation operations concerning each exchange format to a separate DLL, thus making a 'plug-in.' Then, this plug-in could be used together with the CAD/CAM system or with specialized translation software. This approach allows to create new translators rapidly and to gain the reliable, high-efficiency, and reusable program code. The second part of the paper concerns the possible problems of translators' development. These difficulties often come from the exchange standards' misunderstanding or ambiguity in standards. All examples come from the authors' practice experiences of dealing with CAD/CAM systems.