• Communications of the Korean Mathematical Society
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• v.24 no.1
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• pp.127-144
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• 2009

Let f be a diffeomorphism of a compact $C^{\infty}$ manifold, and let p be a hyperbolic periodic point of f. In this paper we introduce the notion of $C^1$-stable inverse shadowing for a closed f-invariant set, and prove that (i) the chain recurrent set $\cal{R}(f)$ of f has $C^1$-stable inverse shadowing property if and only if f satisfies both Axiom A and no-cycle condition, (ii) $C^1$-generically, the chain component $C_f(p)$ of f associated to p is hyperbolic if and only if $C_f(p)$ has the $C^1$-stable inverse shadowing property.

• Journal of Animal Science and Technology
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• v.48 no.6
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• pp.847-860
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• 2006

This study investigated the effects of feeding Charolais steers on diets rich in either n-6 or n-3 polyunsaturated fatty acids (PUFA) and time on feed (TOF) on muscle fatty acid composition and content. Twenty eight steers were fed on ad libitum forage and one of two concentrates varying in the source of fat; soya (high in C18:2 n-6) or whole linseed (high in C18:3 n-3) for either 60 or 90 days in IGER (Institute of Grassland and Environmental Research, UK). The concentrates were fed at approximately 0.73 of total DM intake. TOF influenced carcass weight, conformation and fatness scores, which were higher at 90 v. 60 days (P<0.05). Diet did not affect total fatty acid content of neutral lipid in m. longissimus thoracis but feeding linseed increased total phospholipid fatty acid by approx- imately 15%(P<0.05). Linseed increased the amount and proportion of C18:3 n-3 (P<0.001) and the proportion of CLA (cis-9, trans-11 conjugated linoleic acid), while soya increased the content (P<0.05) and proportion (P<0.001) of C18:2 n-6 in muscle neutral lipid. In muscle phospholipid, linseed significantly increased the amount of CLA, C18:3 n-3 and its longer chain derivatives as well as C14:0, C16:0, C18:0. C18:1 trans and C18:2 n-6. The amount and proportion of C18:2 n-6 and its longer chain C20 derivatives were higher on feeding soya. TOF (90 v. 60 day) increased the content of C14:0, C16:0, C16:1, CLA, C18:1 n-9, C18:2 n-6 and C18:3 n-3 in muscle neutral lipid. The P:S was not affected by diet or TOF. The ratio of C18:2 n-6 : C18:3 n-3 and sum of n-6 : n-3 fatty acids were higher in muscle from animals fed on linseed v. soya (P<0.001). The study indicates that the PUFA composition of beef muscle may be significantly modified by feeding contrasting dietary lipids, soya vs. linseed. Feeding linseed produced a better balance of muscle fatty acids, more in line with current nutritional recommendations with a lower C18:2 n-6:C18:3 n-3 ratio associated with higher muscle content of C18:3 n-3 and C20:5 n-3 and CLA and lower C20:4 n-6.

• Journal of the Korean GEO-environmental Society
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• v.13 no.2
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• pp.95-102
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• 2012

In this study, practical verifications for an advanced C.I.P(Cast in Place Pile) construction method were carried out. The structural characteristics of the method is to attach an angular joint in the steel-casing. This joint plays an important role in boring vertically, connected pile to pile, and protects the permeation of the ground water. For verifications, experimental research and numerical analysis were performed. In the experimental research, two model-tests were set up with the real scale steel-casing. One is to examine the leakage in the joint of piles and the other is to compare earth pressures in the front and the joint, respectively. In addition, 3 point bending test and compressive loading test were carried out and numerical analysis was performed to simulate the loading test. As a result of model-tests, the leakage in the pile joints was not shown up to 300 KPa of water pressure and stress concentration in the joint is out of the question. From the results of bending and compressive test, it was found that the new advanced C.I.P method is more convenient and superior than the conventional method.

• Korean journal of food and cookery science
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• v.16 no.4
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• pp.363-366
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• 2000

This study was attempted to compare the properties of proteolytic enzymes in fruits(Pear, Kiwifruit, Fig, Pineapple and Papaya) for the application of scientific information to cooking. The results were as follows: 1. The optimum temperature of crude proteolytic enzymes in pear, fig and pineapple is 60$^{\circ}C$ and it was relatively active in 40-70$^{\circ}C$; papaya showed max. activity in 60$^{\circ}C$ and highly stable activity in 40-80$^{\circ}C$, but kiwifruit showed max. activity in 40$^{\circ}C$ and it maintained to 70$^{\circ}C$. 2.. The crude proteolytic enzymes of pear, fig, pineapple and papaya showed opt. pH at pH 7.0 and maintained at pH 5.0-8.0, but max. activity of kiwifruit observed in pH 3.0 and pH 5.0-8.0. 3. As a result of comparison of total activities of fruits per kg unit, the order of activities was pineapple > kiwifruit > papaya > fig > pear.

• Korean Journal of Poultry Science
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• v.23 no.4
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• pp.209-219
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• 1996

This research was carried out to get the basic information on the current situation of personal computer utilization for poultry farm management, and the results obtained could be used in developing of the software system for the poultry farm management. Survey method by interview and mail was adopted to carry out the research, and the total number of data used to statistical analyses was k6 poultry farmers. 1. The ratio of the poultry farmers who had the personal computer(P/C) was about 25%, and the most of the layer farmers from age 30 to 40 who manage the flock size 20,000 or above had the P/C. 2. About 70% of the farmers who had P/C have bought the system after 1993, and 83% of those farmers had the P/C-486 or above level system. They bought the P/C with intent to apply it to farm management(39%) and education for children(31%). 3. About 50% of the farmers who had P/C obtained the relevant knowledge for operating computer system by themselves, and the 33% of the P/C-possessed farmers used P/C for word-processor. 4. About 35% of the farmers who had P/C applied their system to analyse the management analyses, and the sarne ratio of the farmers answered that they didn't apply the system to their farm management. 5. About 25% of the farmers who had P/C used package software, and the company which installed the automated cage system offered the software. 6. Most of the farmers considered the computerization of the farm management positively, and they answered that they will apply P/C to their management actively from now on. 7. About 56% of the farmers didn't think that they had sufficient knowledge for operating computer system, and a quarter of them pointed that the difficulty of the buying software for poultry farm management was the most biggest problem to spread out the computerization in the poultry farm. 8. Most of the poultry farmers didn't buy P/C because they didn't have the relevant knowledge for computer system, but they wanted to learn basic knowledge for computer, and they had the intention to apply computer to their management positively. In order to expand the computerization for poultry farm management, appropriate education system for the farmers from age 30 to 40 who manage the flock size 20,000 or above and the development of the software that could be applied to poultry farm management on the spot efficiently should be made.

• Journal of Chest Surgery
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• v.17 no.1
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• pp.157-166
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• 1984

These biologic test procedures are designed to test the suitability of P.V.C. made in Korea intended for parenteral preparation, which were based on the U.S. Pharmacopeia XIX "Biologic Test-Plastic Container", Official from July 1, 1975. Healthy adult human blood and rabbits weighing 2\ulcorner.2Kg were used for test materials. Sample P.V.C. were sampled from the medical equipments made in Korea randomly and Control P.V.C. were sampled from the standardized Cobe and Polystan P.V.C. tubes. P.V.C. extract was prepared from a homogeneous P.V.C. samples by incubating 60 square centimeters of the sample per 20 millimeters of sterile pyrogen-free saline at 70\ulcorner for 72 hours or autoclaving at 120\ulcorner for 1 hour. The Implantation Test was designed to evaluate the reaction of living tissue to the plastic by the method of the implantation of the Sample itself into animal tissue. The Systemic Injection Test, the Intracutaneous Test, and the remainders were designed to determine the biological response of animals to plastics by the single-dose injection of specific extracts prepared from a Sample. The results are as follows; 1.Implantation Test - No significant difference for reactions was noted between the Sample treated animal and the Control after 72 hours of implantation. 2.Systemic Toxicity Injection Test - No sign of toxicity and/or death immediately after injection and at 4, 24, 48 hours respectfully after injection. 3.Intracutaneous Test - None of the animals treated with the Sample showed a significantly greater reaction than the observed in the animals treated with Blank. 4.Pyrogen Assay-Only one animal treated with the Sample showed the maximal rise of rectal temperature about 0.2\ulcorner after 3 hours of injection, but remainders showed no change. 5.Hemolytic Index - The positive Control tube of distilled water exhibited complete hemolysis while the negative Control tube and P.V.C. extract were negative demonstrating no hemolysis. 6.Cell Morphology of Erythrocytes and Leukocytes on Stored, Heparinized Human Blood -- There was no significant difference in the morphology of either the Control or Sample extract. 7.Clotting Mechanism of Human Blood in vitro - After allowing to the P.V.C. extract at room temperature for 5 Hours and at 10\ulcorner for 24 hours, there was no appreciable difference in Prothrombin Time under these conditions. 8.Clotting Mechanism of Rabbit in vivo - At the termination of 5 days after intraperitoneal injection of the P.V.C. extract, no significant changes in Clotting Time were observed. According to the above results, it could be concluded that the P.V.C. made in Korea was acceptable for parenteral preparation, especially treated with physiologic saline and/or human blood.man blood.

• Journal of Microbiology
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• v.40 no.1
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• pp.20-25
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• 2002

An extracellular PHB depolymerase was purified from P. simplicissimum LAR13 cultural medium by Sepharose CL-6B chromatography. When the fungus was grown in a basal salt medium with poly(3-hydroxybutyrate) (PHB) as the sole carbon source, PHB depolymerase production reached maximum at its stationary phase. The mycelial growth rate was higher at 37$^{\circ}C$ than at 30$^{\circ}C$ and even higher than at 25$^{\circ}C$, However, the enzyme production was lower at 37$^{\circ}C$ than 30$^{\circ}C$ or 25$^{\circ}C$. The isolated enzyme is composed of a single polypeptide chain with a molecular mass of about 36 kDa as determined by SDS-PAGE. The optimum conditions for the enzyme activity are pH 5.0 and 45$^{\circ}C$. The enzyme was stable for 30 min at a temperature lower than 50$^{\circ}C$, and stable at pH higher than 2.0 but it was unstable at pH 1.0.1 mM Fe$\^$2+/ reduced the enzyme activity by 56% and the enzyme was inhibited almost completely by 4 mM Fe$\^$2+/ . The enzyme was partially inhibited by phenylmethylsulfonyl fluoride and was very sensitive to diazo-DL-norleucine methyl esters dithiothreitol and mercuric ion. However, N-p - tosyl - L - Iysinechloromethyl ketone, p -hydroxymercuricbenzoate and N- acetylimidazole had no influence upon its activity.

• Microbiology and Biotechnology Letters
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• v.16 no.3
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• pp.193-198
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• 1988

Catalase from Aspergillus niger KUF-04 was purified by five steps including gel filtration. The overall purification gave 64-fold purified preparation, a yield of about nine percent. The enzyme showed its maximum absorption at 406 nm. The optimum pH and temperature for the enzyme activity were around pH 7.0 and 6$0^{\circ}C$, respectively. The catalase was found to be stable in the range of pH 4.0 to pH 8.3 and temperature 2$0^{\circ}C$ to 6$0^{\circ}C$. However, it lost nearly all of the activity by heating at 8$0^{\circ}C$ for 20 min. The activity was markedly inhibited by hydroxylamine, potassium cyanide and sodium azide.

• Journal of Applied Biological Chemistry
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• v.61 no.1
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• pp.59-67
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• 2018

This study was conducted to isolate the cellulolytic bacteria able to grow on LB- Carboxymethyl cellulose (CMC) agar trypan blue medium from the mixed forest and Larix leptolepis stands. Three bacterial strains with high activity against both CMC and xylan were isolated. Both API kit test and 16S rRNA gene sequence analysis revealed that the three different isolates belong to the gene Bacillus. Therefore, the isolates named as Bacillus sp. EFL1, Bacillus sp. EFL2, and Bacillus sp. EFP3. The optimum growth temperature of Bacillus sp. EFL1, EFL2, and EFP3 were $37^{\circ}C$. The optimum temperature for CMCase and xylanase from Bacillus sp. EFL1 were $50^{\circ}C$. The optimum pH of Bacillus sp. EFL1 xylanase was pH 5.0 but the optimum pH of CMCase from Bacillus sp. EFL1 was pH 6.0. The optimum temperature of CMCase and xylanase from Bacillus sp. EFL2 was $60^{\circ}C$, respectively. The optimum pH of CMCase of Bacillus sp. EFL2 was 5.0, whereas xylanase showed high activity at pH 3.0-9.0. The optimum temperature for CMCase and xylanase of Bacillus sp. EFP3 was $50^{\circ}C$. The optimum pH for CMCase and xylanse was 5.0 and 4.0, respectively. CMCases from Bacillus sp. EFL1, EFL2, and EFP3 were thermally unstable. Although xylanase from Bacillus sp. EFL1 and EFP3 showed to be thermally unstable, xylanase from Bacillus sp. EFL2 showed to be thermally stable. Therefore, Bacillus sp. EFL2 has great potential for animal feed, biofuels, and food industry applications.

• Journal of Microbiology and Biotechnology
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• v.11 no.2
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• pp.333-336
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• 2001

An isolated phbC gene from Alcaligenes latus was reintroduced into the parent A. latus through the transformation process, and the effect of the amplified phbC gene on the biosynthesis of P(3-hydroxybutyrate-3-hydroxyvalerate) [P(3HB-3HV)] and P(3-hydroxybutyrate-4-hydroxybutyrate) [P(3HB-4HB)] in the transformant A. latus was investigated. The biosynthesis rate and content of the above copolymers increased up to 1.3-fold after enforcing its own phbC gene, and the molar fractions of 3HV and 4HB in P(3HB-3HV) and P(3HB-4HB) also changed remarkably from 35.0 to 48.0% and from 34.0 to 56.0%, respectively, showing a critical role of PHB synthase which catalyzes the polymerizing reactions between eiher 3HV or 4HB from precursor compounds and 3HB.