• Title/Summary/Keyword: c-KIT

검색결과 576건 처리시간 0.026초

C-kit Expressing Male Germ Cells were Highly Sensitive to Busulfan Treatment and Apoptosis of Male Germ Cells Induced by Busulfan Treatment was not Caused by Fas/FalsL or p53

  • Ok Do-Won;Lee Mi-Suk;Gwon Deuk-Nam;Kim Jin-Hoe
    • 한국동물번식학회:학술대회논문집
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    • 한국동물번식학회 2002년도 춘계학술발표대회 발표논문초록집
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    • pp.4-4
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    • 2002
  • Male germ cell apoptosis has been extensively explored in rodent. In contrast, very little is known about their susceptibility to apoptosis stimuli of developing germ cell stages at the time when germ cell depletion after busulfan treatment occurs. Furthermore, it is still unanswered how spermatogonial stem cells are resistant to busulfan treatment. Spontaneous apoptosis of germ cells was observed in the testis of adult mice and experimentally induced busulfan treated mice increased this apoptosis to such an extent that there was a decrease in the weight of the testis. (omitted)

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휴대용 하부요로기능 검사 장치의 설계 (Design of ambulatory urodynamics monitoring system)

  • 이상옥;김거식;윤대영;서정환;송철규
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 2005년도 심포지엄 논문집 정보 및 제어부문
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    • pp.48-50
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    • 2005
  • Urodynamics describes a collection of tests designed to evaluate lower urinary tract function and can be performed using retrograde filling of the bladder within a room. In this study, we designed and calibrated the potable urodynamics monitoring system using DSP chip (TMS320VC33, Texas Instrument$^{TM}$, U.S.) and collected pressure and EMG using calibration kit (DPT9022K0122, Medtronics$^{TM}$, U.S.). This system can make patients more comportable and monitor spontaneous urination during daily life.

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3차원 그래픽을 이용한 설비 유지보수 시각화 프로그램 개발 (Development of a 3D-Graphics Based Visualization Application for power equipments Maintenance)

  • 정홍석;박창현;장길수
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 2006년도 추계학술대회 논문집 전력기술부문
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    • pp.246-248
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    • 2006
  • This paper presents a visualization application using 3D-graphics for effective maintenance of power equipments. The maintenance algorithm implemented in the application is based on Condition-Based Maintenance (CBM) and Reliability-Centered Maintenance (RCM). The main frame of the developed application was made up based on visual C++ (MFC). In order to develop the interactive 3D application, the WorldToolKit library based on Open GL was used. The developed application can help the power system operators to intuitively recognize the present state and maintenance information of the equipments.

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수종(數種) 한약재의 콜라겐 생합성 효과에 대한 실험적 연구 (Experimental study about the effect of several herbs on collagen synthesis)

  • 윤지은;김남권;황충연;조은희;박민철
    • 한방안이비인후피부과학회지
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    • 제23권3호
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    • pp.33-41
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    • 2010
  • Background & Objective : This study was performed to find a new anti-aging, anti-wrinkle agent. Ten kinds of hers were evaluated by proliferation, cytotoxicity, collagen synthesis tests in cultured human dermal fibroblast. Methods : MTT assay was carried out to check cell proliferation, cytotoxic effect of each herbal medicine. Collagen synthesis was investigated by the amount of propeptide using procollagen type-Ⅰ C peptide(PIP) ELISA kit(Takara). Results and Conclusions : There are no cytotoxicity in all group herbs by MTT assay. Collagen synthetic ability was greatest in LYCII FRUCTUS(LF) and was excellent in order of Rehmanniae Radix Preparat(RRP) > Nelumbinis Semen(NS) > Asparai Radix(AR) > Schizandrae Frucus(SF).

유전자 재조합 형광 단백질 발현 동물세포의 고정화 및 바이오센서의 개발

  • 이정은;구만복
    • 한국생물공학회:학술대회논문집
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    • 한국생물공학회 2002년도 생물공학의 동향 (X)
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    • pp.53-56
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    • 2002
  • Mammalian cell based biosensor kits are expected to be in assessment of samples toxicity more sensitive and accurate. A recombinant fluorescent Chinese Hamster Ovary (CHO) cell line was known to be responsive to the various toxicants Specially. KFC- AlO cell line. which contain the c-fos SRE::GFP plasmid (pKFG). was found to be able to detect toxicants sensitively. A biosensor kit was developed by using an immobilized KFC-A10 cell line. Immobilized recombinant fluorescent cells within agarose, known as a representative hydrogel matrix, have been maintained in the matrix viably and have shown constant fluorescent levels for long time. Immobilized cells have shown the ability to detect the chemical toxicity in the keep of fluorescent level as the metabolism is inhibited under toxic conditions.

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돈분에서 분리한 Candida utilis의 생균제로서의 특성

  • 조진국;최진영;유숙진;허강칠
    • 한국축산식품학회:학술대회논문집
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    • 한국축산식품학회 2005년도 정기총회 및 제35차 춘계 학술 발표대회
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    • pp.285-289
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    • 2005
  • 본 연구는 생균제로 이용할 수 있는 균주개발을 목적으로 돈분에서 성장능력과 내산성이 우수한 Candida sp. 를 분리하였고, API kit를 이용하여 동정하였을 때 Candida utilis로 확인되었다. Candida utilis는 cellulase, phytase활성이 높은 것으로 관찰되었으며, PDB배지에서 18시간 배양시 $6.5{\times}109\;cfu/ml$로 최대로 성장하는 것으로 나타났다. 또, pH 1.5에서도 약 22%의 미생물수($1.34{\times}107\;cfu/ml$)가 잔존하여, 내산성이 강한 것으로 나타났다. 또, $60^{\circ}C$이상의 고온에서는 사멸하였으나 정상적인 생체온도에서는 열 안전성이 있는 것으로 나타났다. 이상의 결과로부터 분리한 Candida utilis는 생균제 및 단세포단백질 생산에 충분히 이용가치가 있는 것으로 확인되었다.

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Proteomic Analysis of Coprinopsis cinerea under Conditions of Horizontal and Perpendicular Gravity

  • Kim, Ji-Su;Kwon, Young-Sang;Bae, Dong-Won;Kwak, Youn-Sig;Kwack, Yong-Bum
    • Mycobiology
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    • 제45권3호
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    • pp.226-231
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    • 2017
  • Coprinopsis cinerea was employed to investigate the fungal response to gravity. Mycelium growth revealed a consistent growth pattern, irrespective of the direction of gravity (i.e., horizontal vs. perpendicular). However, the fruiting body grew in the direction opposite to that of gravity once the primordia had formed. For the proteomic analysis, only curved-stem samples were used. Fifty-one proteins were identified and classified into 13 groups according to function. The major functional groups were hydrolases and transferases (16%), signal transduction (15%), oxidoreductases and isomerases (11%), carbohydrate metabolism (9%), and transport (5%). To the best of our knowledge, this is the first report on a proteomic approach to evaluate the molecular response of C. cinerea to gravity.

Isolation and Characterization of Lactobacillus buchneri Strains with High ${\gamma}$-Aminobutyric Acid Producing Capacity from Naturally Aged Cheese

  • Park, Ki-Bum;Oh, Suk-Heung
    • Food Science and Biotechnology
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    • 제15권1호
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    • pp.86-90
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    • 2006
  • Two lactic acid bacteria (LAB) with high ${\gamma}$-aminobutyric acid (GABA)-producing capacity were isolated from naturally aged cheese. Examination of the biochemical features using an API kit indicated that the two strains belonged to Lactobacillus. They were gram positive, rod-type bacteria, and fermented arabinose, melezitose, melibiose and xylose, but did not utilize cellobiose or trehalose. 16S rDNA sequencing analysis confirmed that they were Lactobacillus buchneri and Lactobacillus sp. They were accordingly named as Lactobacillus buchneri OPM-1 and Lactobacillus sp. OPM-2, and could produce GABA from MRS broth supplemented with 10 g/L of monosodium glutamate (MSG) at a productivity of 91.7 and 116.7 mg/L/hr, respectively. Cell extracts of L. buchneri OPM-1 and Lactobacillus sp. OPM-2 showed glutamate decarboxylase (GAD) activity, for which the optimum pH and temperature were 5.5 and $30^{\circ}C$, respectively.

FMI 표준을 활용한 관절형 로터/공력 연계시뮬레이션 (Articulated Rotor/Aerodynamics Co-Simulation Using FMI Standard)

  • 백승길;박중용
    • 항공우주시스템공학회지
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    • 제9권4호
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    • pp.1-7
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    • 2015
  • The purpose of this research is to develop co-simulation methodology of codes developed in different modeling and simulation environment. We develop aerodynamic FMU(Functional Mock-up Unit) meeting FMI(Functional Mock-up Interface) specification version2 utilizing Legacy FORTRAN aerodynamic code based on unsteady vortex lattice method. It is concluded that making FMU is possible utilizing Legacy code made in any language which can be compiled and linked with object in FMI API coded in C language. This paper explains QTronic's method of using FMU SDK(Software Development Kit) and suggestion for using FORTRAN properly. Finally, we make articulated rotor/aerodynamics co-simulation by integrating aerodynamics FMU and rotor FMU developed by Modelica.

A Case of Small Bowel GIST Initially Suspected as Peritoneal Seeding of Gastric Cancer

  • Jo, Dae-Hyeun;Song, Jeong-Yoon;Kim, Yong-Ho
    • Journal of Gastric Cancer
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    • 제10권3호
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    • pp.137-140
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    • 2010
  • Gastrointestinal stromal tumors (GISTs) constitute the most common primary mesenchymal tumors of the digestive tract and characteristically express c-kit (CD117). GISTs are the most common non-epithelial tumor of the GI tract and frequently originate from the stomach and small bowel. Specifically, the synchronous occurrence of a GIST with other epithelial tumors is rarely reported. Recently, we discovered one case of a concurrent gastric cancer and a small bowel GIST that was initially suspected to be peritoneal seeding from gastric cancer. The patient was initially admitted with epigastric pain. Gastric cancer with peritoneal seeding was suspected after an evaluation. Following a laparoscopic examination, a distal gastrectomy with D2 lymph node dissection and small-intestine segmental resection was performed. The final pathologic diagnosis was early gastric cancer and high-risk small bowel GIST. The patient refused adjuvant therapy for the GIST, and currently shows no other marked indisposition. He has been disease-free for 14 months.