Experimental study about the effect of several herbs on collagen synthesis

수종(數種) 한약재의 콜라겐 생합성 효과에 대한 실험적 연구

  • 윤지은 (원광대학교 한의과대학 안이비인후피부과) ;
  • 김남권 (원광대학교 한의과대학 안이비인후피부과) ;
  • 황충연 (원광대학교 한의과대학 안이비인후피부과) ;
  • 조은희 (원광대학교 한의과대학 침구과) ;
  • 박민철 (원광대학교 한의과대학 안이비인후피부과)
  • Received : 2010.11.07
  • Accepted : 2010.12.04
  • Published : 2010.12.25


Background & Objective : This study was performed to find a new anti-aging, anti-wrinkle agent. Ten kinds of hers were evaluated by proliferation, cytotoxicity, collagen synthesis tests in cultured human dermal fibroblast. Methods : MTT assay was carried out to check cell proliferation, cytotoxic effect of each herbal medicine. Collagen synthesis was investigated by the amount of propeptide using procollagen type-Ⅰ C peptide(PIP) ELISA kit(Takara). Results and Conclusions : There are no cytotoxicity in all group herbs by MTT assay. Collagen synthetic ability was greatest in LYCII FRUCTUS(LF) and was excellent in order of Rehmanniae Radix Preparat(RRP) > Nelumbinis Semen(NS) > Asparai Radix(AR) > Schizandrae Frucus(SF).



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