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대전광역시 월평공원과 전북 격포해수욕장 주변 야생화와 토양들로부터 야생효모의 분리 및 국내 미기록 효모들의 특성과 생리 활성 (Isolation of Wild Yeasts and Characterization of Physiological Functionalities of Unrecorded Wild Yeasts Obtained from Flowers and Soils of the Wolpyung Park, Daejeon City and Gykpo Beach, Buan, Jeollabuk-do in Korea)

  • 장지은;박선정;이종수
    • 한국균학회지
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    • 제49권1호
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    • pp.87-100
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    • 2021
  • 많은 사람들이 다녀가는 도시공원과 해변 환경의 효모 특성을 알아보기 위해 대전광역시 월평공원 갑천 누리길과 내원사 주변의 야생화와 토양 75점에서 모두 88균주의 야생 효모들을 분리, 동정하였다. 또한 전북 부안의 격포해수욕장과 격포항 주변 야생화와 토양 35점에서 30균주의 야생 효모들을 분리하였다. 이들 분리 야생 효모들 중 A. porosum ASCM32-1, A. scarabaeorum WP49-1, C. athensensis WP4-90-3, C. berkhoutiae WP34-2, C. bracarensis WP35-1, Cy. amylophila GP19-1, K. gamospora WP9-1, M. carpophila BN-17-1, T. namnaoensis WP4-89-1, V. taibaiensis WP13-2 등 10종의 국내 미기록종 후보 균주들을 선별하여 균학적 특성을 확인하였다. 이들 미기록 효모들은 주로 구형~타원형으로 모두 출아방식으로 영양 증식을 하였고 V. taibaiensis WP13-2와 K. gamospora WP9-1, C. athensensis WP4-90-3 외 7종의 미기록 효모들은 자낭포자를 형성하지 않은 무포자 효모들이었으며A. porosum ASCM32-1만이 의균사를 생성하였다. 대부분의 균주들은 YPD 배지와 YM 배지에서생육하였고V. taibaiensis WP13-2와K. gamospora WP9-1, T. namnaoensis WP4-89-1 균주들은 15% NaCl을 함유한 YPD 배지에서 생육하는 호염성 효모들이었고, Cy. amylophila GP19-1 균주는 40℃에서도 생육하였다. C. athensensis WP4-90-3 균주 등 5균주들이 10% 에탄올 내성을 보였고C. athensensis WP4-90-3 균주는 Li2+ 등 많은 중금속들에 대해서 내성을 보였다. 국내 미기록 효모들과 비병원성 야생 효모 균주 무세포추출물들의 β-glucuronidase 저해 활성은 C. oleophila WP5-19-1의 무세포 추출물이 49.0%로 가장 높았고 미기록 효모인 K. gamospora WP9-1의 무세포 추출물도 27.7%를 보였다. 호중구 elastase 저해 활성은 W. anomalus HO9-2가 38.4%로 우수하여 건강 소재로 생산에 매우 유용한 균주로 활용될 것으로 기대된다.

4종 아크릴릭 레진 인공치의 물리적 성질에 관한 비교 (COMPARISON OF THE PHYSICAL PROPERTIES OF FOUR KINDS OF ACRYLIC RESIN DENTURE TEETH)

  • 황종우;정재헌;고영무
    • 대한치과보철학회지
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    • 제33권2호
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    • pp.210-230
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    • 1995
  • To compare the wear resistance of four kinds of commercial acrylic resin teeth [SR-Orthosit PosterioresR(Ivoclar Co., Liechtenstein), Endura PosteriorR(Shofu Inc. Japan), trubyte IPN teethR(Dentsply International Inc., York,), Trubyte BiotoneR(dentsply Inermational Inc. Brazil) by means of the toothbrush abrasion method, the artificial resin teeth were embedded in epoxy resin with the occlusal surfaces aligned in one plane for a total of 40 blocks. There after, each lock was mounted in the arm of the toothbrush abrasion machine(K 236, Japan). Wear measurements were made on the three preconditioned states. Those were as follows : no treatment specimens, thermocycled specimens, and thermocycled specimens which were immeresed applied load of 400g during the buring the brushing cycle. At the end of the 30,000-stroke cycle, each specimen was removed, and weighed. The microhardness of four kinds of commercial resin teeth were determined by means of microhardness tester. Microhardness tests were performed on te no treatment specimens, thermocycled specimens, and thermocycled specimens with immersion in the denture cleansing solution. Finally, the comparison of thermal properties were perfomed using differential scanning calorimeter(DSC-1500). The following results were obtained : 1. In the case of no treatment teeth, the wear amount of endura Posterior(EN) was the greatest among the others(p<0.01), and the wear amount of three kinds of artificial resin teeth was increased in the order of Trubyte IPN teeth(IN), Trubyte Biotone(BN), SR-Orthosit Posteriores(SN) but there was no statistic significance(p>0.01). 2. The wear amount of Trubyte IPN teeth(IT) and Trubyte Biotone(BT), was increased due to thermocycling effect, but that of Endura Posteriores(ET) was decreased conversely(p<0.01). 3. Except for the SR-Orthosit Posteriores(STC), the wear amount of three kinds of artificial resing teeth(that is, ETC, ITC, BTC) was increased due to denture cleansing solution$(Cledent^R)$, but there was no statistic significance(p>0.01). But the wear amount of the SR-Orthosit Posteriores(STC) was the greatest among the others(p<0.01). 4. The wear amount of toothbrush was the greatest in case of contact with occlusal surface of SROrthosit Posteriores resin teeth(p<0.01). 5. the microhardness values(KHN) of the SR-Orthosit Posteriores was the highest among the experimental artificial resin teeth(p<0.01). 6. There was no relationship between microhardness valuse(KHN) and wear amount of four kinds of experimental artificial resin teeth(p<0.01). 7. The differential canning calorimetric property of four kinds of artificial resin teeth did not show endothermal or exothermal peak in the range of $100^{\circ}C$

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Predicting Stock Liquidity by Using Ensemble Data Mining Methods

  • Bae, Eun Chan;Lee, Kun Chang
    • 한국컴퓨터정보학회논문지
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    • 제21권6호
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    • pp.9-19
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    • 2016
  • In finance literature, stock liquidity showing how stocks can be cashed out in the market has received rich attentions from both academicians and practitioners. The reasons are plenty. First, it is known that stock liquidity affects significantly asset pricing. Second, macroeconomic announcements influence liquidity in the stock market. Therefore, stock liquidity itself affects investors' decision and managers' decision as well. Though there exist a great deal of literature about stock liquidity in finance literature, it is quite clear that there are no studies attempting to investigate the stock liquidity issue as one of decision making problems. In finance literature, most of stock liquidity studies had dealt with limited views such as how much it influences stock price, which variables are associated with describing the stock liquidity significantly, etc. However, this paper posits that stock liquidity issue may become a serious decision-making problem, and then be handled by using data mining techniques to estimate its future extent with statistical validity. In this sense, we collected financial data set from a number of manufacturing companies listed in KRX (Korea Exchange) during the period of 2010 to 2013. The reason why we selected dataset from 2010 was to avoid the after-shocks of financial crisis that occurred in 2008. We used Fn-GuidPro system to gather total 5,700 financial data set. Stock liquidity measure was computed by the procedures proposed by Amihud (2002) which is known to show best metrics for showing relationship with daily return. We applied five data mining techniques (or classifiers) such as Bayesian network, support vector machine (SVM), decision tree, neural network, and ensemble method. Bayesian networks include GBN (General Bayesian Network), NBN (Naive BN), TAN (Tree Augmented NBN). Decision tree uses CART and C4.5. Regression result was used as a benchmarking performance. Ensemble method uses two types-integration of two classifiers, and three classifiers. Ensemble method is based on voting for the sake of integrating classifiers. Among the single classifiers, CART showed best performance with 48.2%, compared with 37.18% by regression. Among the ensemble methods, the result from integrating TAN, CART, and SVM was best with 49.25%. Through the additional analysis in individual industries, those relatively stabilized industries like electronic appliances, wholesale & retailing, woods, leather-bags-shoes showed better performance over 50%.

지하수 관개에 의한 수도의 멸준양상과 그 방지책에 관한 연구 (Studies on the Rice Yield Decreased by Ground Water Irrigation and Its Preventive Methods)

  • 한욱동
    • 한국농공학회지
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    • 제16권1호
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    • pp.3225-3262
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    • 1974
  • The purposes of this thesis are to clarify experimentally the variation of ground water temperature in tube wells during the irrigation period of paddy rice, and the effect of ground water irrigation on the growth, grain yield and yield components of the rice plant, and, furthermore, when and why the plant is most liable to be damaged by ground water, and also to find out the effective ground water irrigation methods. The results obtained in this experiment are as follows; 1. The temperature of ground water in tube wells varies according to the location, year, and the depth of the well. The average temperatures of ground water in a tubewells, 6.3m, 8.0m deep are $14.5^{\circ}C$ and $13.1^{\circ}C$, respercively, during the irrigation period of paddy rice (From the middle of June to the end of September). In the former the temperature rises continuously from $12.3^{\circ}C$ to 16.4$^{\circ}C$ and in the latter from $12.4^{\circ}C$ to $13.8^{\circ}C$ during the same period. These temperatures are approximately the same value as the estimated temperatures. The temperature difference between the ground water and the surface water is approximately $11^{\circ}C$. 2. The results obtained from the analysis of the water quality of the "Seoho" reservoir and that of water from the tube well show that the pH values of the ground water and the surface water are 6.35 and 6.00, respectively, and inorganic components such as N, PO4, Na, Cl, SiO2 and Ca are contained more in the ground water than in the surface water while K, SO4, Fe and Mg are contained less in the ground water. 3. The response of growth, yield and yield components of paddy rice to ground water irrigation are as follows; (l) Using ground water irrigation during the watered rice nursery period(seeding date: 30 April, 1970), the chracteristics of a young rice plant, such as plant height, number of leaves, and number of tillers are inferior to those of young rice plants irrigated with surface water during the same period. (2) In cases where ground water and surface water are supplied separately by the gravity flow method, it is found that ground water irrigation to the rice plant delays the stage at which there is a maximum increase in the number of tillers by 6 days. (3) At the tillering stage of rice plant just after transplanting, the effect of ground water irrigation on the increase in the number of tillers is better, compared with the method of supplying surface water throughout the whole irrigation period. Conversely, the number of tillers is decreased by ground water irrigation at the reproductive stage. Plant height is extremely restrained by ground water irrigation. (4) Heading date is clearly delayed by the ground water irrigation when it is practised during the growth stages or at the reproductive stage only. (5) The heading date of rice plants is slightly delayed by irrigation with the gravity flow method as compared with the standing water method. (6) The response of yield and of yield components of rice to ground water irrigation are as follows: \circled1 When ground water irrigation is practised during the growth stages and the reproductive stage, the culm length of the rice plant is reduced by 11 percent and 8 percent, respectively, when compared with the surface water irrigation used throughout all the growth stages. \circled2 Panicle length is found to be the longest on the test plot in which ground water irrigation is practised at the tillering stage. A similar tendency as that seen in the culm length is observed on other test plots. \circled3 The number of panicles is found to be the least on the plot in which ground water irrigation is practised by the gravity flow method throughout all the growth stages of the rice plant. No significant difference is found between the other plots. \circled4 The number of spikelets per panicle at the various stages of rice growth at which_ surface or ground water is supplied by gravity flow method are as follows; surface water at all growth stages‥‥‥‥‥ 98.5. Ground water at all growth stages‥‥‥‥‥‥62.2 Ground water at the tillering stage‥‥‥‥‥ 82.6. Ground water at the reproductive stage ‥‥‥‥‥ 74.1. \circled5 Ripening percentage is about 70 percent on the test plot in which ground water irrigation is practised during all the growth stages and at the tillering stage only. However, when ground water irrigation is practised, at the reproductive stage, the ripening percentage is reduced to 50 percent. This means that 20 percent reduction in the ripening percentage by using ground water irrigation at the reproductive stage. \circled6 The weight of 1,000 kernels is found to show a similar tendency as in the case of ripening percentage i. e. the ground water irrigation during all the growth stages and at the reproductive stage results in a decreased weight of the 1,000 kernels. \circled7 The yield of brown rice from the various treatments are as follows; Gravity flow; Surface water at all growth stages‥‥‥‥‥‥514kg/10a. Ground water at all growth stages‥‥‥‥‥‥428kg/10a. Ground water at the reproductive stage‥‥‥‥‥‥430kg/10a. Standing water; Surface water at all growh stages‥‥‥‥‥‥556kg/10a. Ground water at all growth stages‥‥‥‥‥‥441kg/10a. Ground water at the reproductive stage‥‥‥‥‥‥450kg/10a. The above figures show that ground water irrigation by the gravity flow and by the standing water method during all the growth stages resulted in an 18 percent and a 21 percent decrease in the yield of brown rice, respectively, when compared with surface water irrigation. Also ground water irrigation by gravity flow and by standing water resulted in respective decreases in yield of 16 percent and 19 percent, compared with the surface irrigation method. 4. Results obtained from the experiments on the improvement of ground water irrigation efficiency to paddy rice are as follows; (1) When the standing water irrigation with surface water is practised, the daily average water temperature in a paddy field is 25.2$^{\circ}C$, but, when the gravity flow method is practised with the same irrigation water, the daily average water temperature is 24.5$^{\circ}C$. This means that the former is 0.7$^{\circ}C$ higher than the latter. On the other hand, when ground water is used, the daily water temperatures in a paddy field are respectively 21.$0^{\circ}C$ and 19.3$^{\circ}C$ by practising standing water and the gravity flow method. It can be seen that the former is approximately 1.$0^{\circ}C$ higher than the latter. (2) When the non-water-logged cultivation is practised, the yield of brown rice is 516.3kg/10a, while the yield of brown rice from ground water irrigation plot throughout the whole irrigation period and surface water irrigation plot are 446.3kg/10a and 556.4kg/10a, respectivelely. This means that there is no significant difference in yields between surface water irrigation practice and non-water-logged cultivation, and also means that non-water-logged cultivation results in a 12.6 percent increase in yield compared with the yield from the ground water irrigation plot. (3) The black and white coloring on the inside surface of the water warming ponds has no substantial effect on the temperature of the water. The average daily water temperatures of the various water warming ponds, having different depths, are expressed as Y=aX+b, while the daily average water temperatures at various depths in a water warming pond are expressed as Y=a(b)x (where Y: the daily average water temperature, a,b: constants depending on the type of water warming pond, X; water depth). As the depth of water warning pond is increased, the diurnal difference of the highest and the lowest water temperature is decreased, and also, the time at which the highest water temperature occurs, is delayed. (4) The degree of warming by using a polyethylene tube, 100m in length and 10cm in diameter, is 4~9$^{\circ}C$. Heat exchange rate of a polyethylene tube is 1.5 times higher than that or a water warming channel. The following equation expresses the water warming mechanism of a polyethylene tube where distance from the tube inlet, time in day and several climatic factors are given: {{{{ theta omega (dwt)= { a}_{0 } (1-e- { x} over { PHI v })+ { 2} atop { SUM from { { n}=1} { { a}_{n } } over { SQRT { 1+ {( n omega PHI) }^{2 } } } } LEFT { sin(n omega t+ { b}_{n }+ { tan}^{-1 }n omega PHI )-e- { x} over { PHI v }sin(n omega LEFT ( t- { x} over {v } RIGHT ) + { b}_{n }+ { tan}^{-1 }n omega PHI ) RIGHT } +e- { x} over { PHI v } theta i}}}}{{{{ { theta }_{$\infty$ }(t)= { { alpha theta }_{a }+ { theta }_{ w'} +(S- { B}_{s } ) { U}_{w } } over { beta } , PHI = { { cpDU}_{ omega } } over {4 beta } }}}} where $\theta$$\omega$; discharged water temperature($^{\circ}C$) $\theta$a; air temperature ($^{\circ}C$) $\theta$$\omega$';ponded water temperature($^{\circ}C$) s ; net solar radiation(ly/min) t ; time(tadian) x; tube length(cm) D; diameter(cm) ao,an,bn;constants determined from $\theta$$\omega$(t) varitation. cp; heat capacity of water(cal/$^{\circ}C$ ㎥) U,Ua; overall heat transfer coefficient(cal/$^{\circ}C$ $\textrm{cm}^2$ min-1) $\omega$;1 velocity of water in a polyethylene tube(cm/min) Bs ; heat exchange rate between water and soil(ly/min)

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면역조직화학염색법을 이용한 흉막의 악성중피종과 전이성 선암의 감별진단 (Differential Diagnosis of Pleural Mesothelioma and Metastatic Adenocarcinoma by Immunohistochemistry)

  • 고경행;박창민;임명수;김유일;장일권;황준화;임성철;김영철;박경옥;박창수
    • Tuberculosis and Respiratory Diseases
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    • 제47권4호
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    • pp.478-487
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    • 1999
  • 연구배경 : 전자현미경적 관찰에 의해 진단된 흉막의 악성중피종과 전이성선암 환자를 대상으로 하여 7종의 단일클론항체를 이용한 면역조직화학염색을 시행하고 이들의 발현양상을 비교하여 두 질환을 감별할 수 있는 가장 효과적인 염색방법을 알아보고자 하였다. 방 법 : 면역조직화학염색법은 Fisher사(Fisher scientific)의 1-hour immunohistochemistry방법을 이용하였다. 종양조직은 포르말린으로 고정된 파라핀 조직을 이용하여, $3{\mu}m$ 두께로 잘라서, $85^{\circ}C$에서 가열시킨 후 파라핀을 제거하고, 희석액과 단클론항체를 $45^{\circ}C$에서 15분간 반응시켰다. 각 단계마다 automation buffer로 실온에서 세척하였고, avidin-biotin-peroxidase system을 이용한 immunoperoxidase method로 염색하였다. 조직절편은 $45^{\circ}C$에서 10분간 색소원반응을 시행하였고, 대조염색은 hematoxylin으로 실시하였다. 조직 절편은 최소한 각기 다른 두 구역에서 세포질 또는 세포막이 분명하게 염색될 때 양성으로 판정하였다. 결 과 : 7종의 단일클론항체중 CK, EMA, vimentin, S-100 단백과 Leu-M1은 악성중피종과 전이성선암에서 발현율의 차이는 없었으나, B72-3은 전이성선암에서만 발현되었고, CEA는 전이성선암 전례와 악성중피종 42.9%에서 발현되어서 민감도는 높았으나 특이도는 낮았다. 결 론 : B72-3을 이용한 면역조직화학염색은 악성중피종과 전이성선암의 감별진단에 가장 유용한 방법으로 시사되었으며, 기존에 사용되고 있는 CEA와 B72-3을 함께 이용한다면 악성중피종과 전이성선암에 대한 감별진단력을 높일 수 있을 것으로 사료된다.

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노천굴착에서 발파진동의 크기를 감량 시키기 위한 정밀파실험식 (On the vibration influence to the running power plant facilities when the foundation excavated of the cautious blasting works.)

  • 허진
    • 화약ㆍ발파
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    • 제9권1호
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    • pp.3-13
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    • 1991
  • 발파에 의한 지반진동의 크기는 화약류의 종류에 따른 화약의 특성, 장약량, 기폭방법, 전새의 상태와 화약의 장전밀도, 자유면의 수, 폭원과 측간의 거리 및 지질조건 등에 따라 다르지만 지질 및 발파조건이 동일한 경우 특히 측점으로부터 발파지점 까지의 거리와 지발당 최대장약량 (W)간에 깊은 함수관계가 있음이 밝혀졌다. 즉 발파진동식은 $V=K{\cdot}(\frac{D}{W^b})^n{\;}{\cdots\cdots\cdots\cdots\cdots\cdots\cdots\cdots\cdots\cdots\cdots}$ (1) 여기서 V ; 진동속도, cm /sec D ; 폭원으로부터의 거리, m W ; 지발 장약량, kg K ; 발파진동 상수 b ; 장약지수 R ; 감쇠지수 이 발파진동식에서 b=1/2인 경우 즉 $D{\;}/{\;}\sqrt{W}$를 자승근 환산거리(Root scaled distance), $b=\frac{1}{3}$인 경우 즉 $D{\;}/{\;}\sqrt[3]{W}$를 입방근환산거리(Cube root scaled distance)라 한다. 이 장약 및 감쇠지수와 발파진동 상수를 구하기 위하여 임의거리와 장약량에 대한 진동치를 측정, 중회귀분석(Multiple regressional analysis)에 의해 일반식을 유도하고 Root scaling과 Cube root scaling에 대한 회귀선(regression line)을 구하여 회귀선에 대한 적합도가 높은 쪽을 택하여 비교, 검토하였다. 위 (1)식의 양변에 log를 취하여 linear form(직선형)으로 바꾸어 쓰면 (2)式과 같다. log V=A+BlogD+ClogW ----- (2) 여기서, A=log K B=-n C=bn (2)식은 다시 (3)식으로 표시할 수 있다. $Yi=A+BXi_{1}+CXi_{2}+{\varepsilon}i{\;}{\cdots\cdots\cdots\cdots\cdots\cdots\cdots\cdots\cdots\cdots\cdots}$(3) 여기서, $Xi_{1},{\;}Xi_{2} ;(두 독립변수 logD, logW의 i번째 측정치. Yi ; ($Xi_1,{\;}Xi_2$)에 대한 logV의 측정치 ${\varepsilon}i$ ; error term 이다. (3)식에서 n개의 자료를 (2)식의 회귀평면으로 대표시키기 위해서는 $S={\sum}^n_{i=1}\{Yi-(A+BXi_{1}+CXi_{2})\}\^2$을 최소로하는 A, B, C 값을 구하면 된다. 이 방법을 최소자승법이 라 하며 S를 최소로 하는 A, B, C의 값은 (4)식으로 표시한다. $\frac{{\partial}S}{{\partial}A}=0,{\;}\frac{{\partial}S}{{\partial}B}=0,{\;}\frac{{\partial}S}{{\partial}C}=0{\;}{\cdots\cdots\cdots\cdots\cdots\cdots\cdots\cdots\cdots\cdots\cdots}$ (4) 위식을 Matrix form으로 간단히 나타내면 식(5)와 같다. [equation omitted] (5) 자료가 많아 계산과정이 복잡해져서 본실험의 정자료들은 전산기를 사용하여 처리하였다. root scaling과 Cube root scaling의 경우 각각 $logV=A+B(logD-\frac{1}{2}W){\;}logV=A+B(logD-\frac{1}{3}W){\;}\}{\;}{\cdots\cdots\cdots\cdots\cdots\cdots\cdots\cdots\cdots}$ (6) 으로 (2)식의 특별한 형태이며 log-log 좌표에서 직선으로 표시되고 이때 A는 절편, B는 기울기를 나타낸다. $\bullet$ 측정치의 검토 본 자료의 특성을 비교, 검토하기 위하여 지금까지 발표된 국내의 몇몇 자료를 보면 다음과 같다. 물론, 장약량, 폭원으로 부터의 거리등이 상이하지만 대체적인 경향성을 추정하는데 참고할수 있을 것이다. 금반 총실측자료는 총 88개이지만 환산거리(5.D)와 진동속도의 크기와의 관계에서 차이를 보이고 있어 편선상 폭원과 측점지점간의 거리에 따라 l00m말만인 A지역과 l00m이상인B지역으로 구분하였다. 한편 A지역의 자료 56개중, 상하로 편차가 큰 19개를 제외한 37개자료와 B지역의 29개중 2개를 낙외한 27개(88개 자료중 거리표시가 안된 12월 1일의 자료3개는 원래부터 제외)의 자료를 computer로 처리하여 얻은 발파진동식은 다음과 같다. $V=41(D{\;}/{\;}\sqrt[3]{W})^{-1.41}{\;}{\cdots\cdots\cdots\cdots\cdots\cdots\cdots\cdots\cdots}$ (7) (-100m)(R=0.69) $V=124(D{\;}/{\;}\sqrt[3]{W})^{-1.66){\;}{\cdots\cdots\cdots\cdots\cdots\cdots\cdots\cdots\cdots}$ (8) (+100m)(R=0.782) 식(7) 및 (8)에서 R은 구한 직선식의 적합도를 나타내는 상관계수로 R=1인때는 모든 측정자료가 하나의 직선상에 표시됨을 의미하며 그 값이 낮을수록 자료가 분산됨을 뜻한다. 본 보고에서는 상관계수가 자승근거리때 보다는 입방근일때가 더 높기 때문에 발파진동식을 입방근($D{\;}/{\;}\sqrt[3]{W}$)으로 표시하였다. 특히 A지역에서는 R=0.69인데 비하여 폭원과 측점지점간의 거리가 l00m 이상으로 A지역보다 멀리 떨어진 B지역에서는 R=0.782로 비교적 높은 값을 보이는 것은 진동성분중 고주파성분의 상당량이 감쇠를 당하기 때문으로 생각된다.

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