Tuberculosis and Respiratory Diseases
- Volume 47 Issue 4
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- Pages.478-487
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- 1999
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- 1738-3536(pISSN)
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- 2005-6184(eISSN)
Differential Diagnosis of Pleural Mesothelioma and Metastatic Adenocarcinoma by Immunohistochemistry
면역조직화학염색법을 이용한 흉막의 악성중피종과 전이성 선암의 감별진단
- Ko, Kyung-Haeng (Department of Internal Medicine, Chonnam National University, Medical School) ;
- Park, Chang-Min (Department of Internal Medicine, Chonnam National University, Medical School) ;
- Rim, Myung-Soo (Department of Internal Medicine, Chonnam National University, Medical School) ;
-
Kim, Yoo-Il
(Department of Internal Medicine, Chonnam National University, Medical School) ;
- Jang, Il-Gweon (Department of Internal Medicine, Chonnam National University, Medical School) ;
- Hwang, Joon-Hwa (Department of Internal Medicine, Chonnam National University, Medical School) ;
-
Lim, Sung-Chul
(Department of Internal Medicine, Chonnam National University, Medical School) ;
- Kim, Young-Chul (Department of Internal Medicine, Chonnam National University, Medical School) ;
-
Park, Kyung-Ok
(Department of Internal Medicine, Chonnam National University, Medical School) ;
- Park, Chang-Soo (Department of Pathology, Chonnam National University, Medical School)
- 고경행 (전남대학교 의과대학 내과학교실) ;
- 박창민 (전남대학교 의과대학 내과학교실) ;
- 임명수 (전남대학교 의과대학 내과학교실) ;
-
김유일
(전남대학교 의과대학 내과학교실) ;
- 장일권 (전남대학교 의과대학 내과학교실) ;
- 황준화 (전남대학교 의과대학 내과학교실) ;
-
임성철
(전남대학교 의과대학 내과학교실) ;
- 김영철 (전남대학교 의과대학 내과학교실) ;
-
박경옥
(전남대학교 의과대학 내과학교실) ;
- 박창수 (전남대학교 의과대학 병리학교실)
- Published : 1999.10.30
Abstract
Background : Differential diagnosis of pleural malignant mesothelioma from secondary metastatic adenocarcinoma is often difficult. A variety of pathologic techniques have been developed to make a differential diagnosis of carcinoma from mesothelioma. Immunohistochemistry detecting diverse antigenic substances such as CEA, Leu-M1, Bn-3, S-100 protein, vimentin, CK and EMA has been claimed to be of value as a panel in the differential diagnosis of adenocarcinoma from mesothelioma. The aim of this study was to investigate the suitable antibodies to distinguish mesothelioma from metastatic adenocarcinoma and establish candidate markers in a panel. Methods : Complete, one-hour immunohistochemical staining using antibodies against cytokeratin (CK), epithelial membrane antigen(EMA), S-100 protein, vimentin, B72-3, Leu-M1, and carcino-embryonic antigen(CEA) was applied to cell blocks from 7 mesotheliomas and 7 adenocarcinomas which were confirmed by electron microscopic and histpathologic methods. Results : All adenocarcinomas and 71.4% of mesotheliomas expressed the cytokeratin and EMA. S-100 protein and vimentin were expressed in 57.1% and 42.9% of mesotheliomas and 14.3% and 28.5% of adenocarcinomas, respectively. B72-3 was expressed in all adenocarcinomas, but in none of mesotheliomas. Leu-M1 was positive in 71.4% of the adenocarcinoma and 14.3% of the mesotheliomas. CEA was positive in all adenocarcinomas and 42.9% of mesotheliomas. Leu-M1 and B72-3 were coexpressed in 71.4% of adenocarcinomas but in none of mesothelioma. B72-3 and CEA were coexpressed in all adenocarcinomas, but in none of mesotheliomas. Conclusion : We concluded that B72-3 immunohistochemistry or panel staining of B72-3 and CEA could be recommanded for the differential diagnosis of pleural mesothelioma from metastatic adenocarcinoma.
연구배경 : 전자현미경적 관찰에 의해 진단된 흉막의 악성중피종과 전이성선암 환자를 대상으로 하여 7종의 단일클론항체를 이용한 면역조직화학염색을 시행하고 이들의 발현양상을 비교하여 두 질환을 감별할 수 있는 가장 효과적인 염색방법을 알아보고자 하였다. 방 법 : 면역조직화학염색법은 Fisher사(Fisher scientific)의 1-hour immunohistochemistry방법을 이용하였다. 종양조직은 포르말린으로 고정된 파라핀 조직을 이용하여,