• Journal of Plant Biotechnology
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• v.6 no.4
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• pp.241-246
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• 2004

The bulb scales and shoot sections ($7\;\cal{mm}\;\times\;15\;\cal{mm}$) of Lilium oriental hybrid 'Casablanca' were cultured to compare bulblet growth in vitro. Shoots were induced from in vitro grown bulbscales on MS medium with $1.0\;\cal{mg/L}\;BA,\;0.5\;\cal{mg/L}$ IAA, and 30 g/L sucrose. The regenerated shoots were cut into shoot sections, and cultured on MS medium with $2.0\;\cal{mg/L}\;BA,\;0.5\;\cal{mg/L}$ IAA and 30 g/L sucrose for shoot proliferation. Culture of shoot sections stimulated bulblet growth significantly than the bulb scales on MS medium with 60 g/L sucrose. However, the bulblets from shoot sections did not reach ideal size to produce stems with several leaves. Therefore, liquid medium was added into the same vessels to stimulate bulblet growth further. After shoot sections were cultured on MS medium with 60 g/L sucrose and 2 g/L activated charcoal for two months in dark, $20\;\cal{ml}$ liquid media containing various concentrations of sucrose and MS salts were added. Two months later, the added liquid medium stimulated bulblet growth remarkably as compared to bulblets grown without added liquid medium. The added $25\;\cal{ml}$ liquid medium containing 120 g/L sucrose and double strength of MS salts were the most effective for growth of in vitro bulblets. More than $94\%$ bulblets produced by this method sprouted stems with several leaves after cold treatment at $5^{\circ}C$ for three months.

• Journal of the Korean GEO-environmental Society
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• v.6 no.4
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• pp.65-71
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• 2005

Recently, difficulties in soft ground improvement that caused by effectiveness of the ground improvement, the durability and environmentally friendliness of the injection material come to the fore. This paper studies the field applicability of the SCM in reinforcement and seepage cutoff of the back of an existing continuous wall. SCM uses double rod which imposes heavy pressure($10-100kgf/cm^2$) to disturbed, cut, discharge, and mix the ground. It is observed that a bulb is formed by using cement paste and environmentally friendly injection materials with minimal alkali leaching. Unconfined compression test and fish poison tests are performed. Test results indicate that the method results in higher durability, less leaching through use of the environmentally friendly injection material, and faster mobilization of the strength. In addition, field tests confirm the formation of the bulb and the seepage cutoff wall.

• Resources Recycling
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• v.16 no.6
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• pp.39-45
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• 2007

Optimum condition for neutralization has been studied to remove the copper and zinc ions from spent sulfate solutions which resulted from the acid washing of the base of brass bulb. Chemical distribution of copper and zinc species and the variation of solubility of the two ions with solution pH were obtained by considering the complex formation reaction and mass balance. Removal percentage of zinc was more sensitive to solution pH than that of copper. This results from the fact that the solubility of zinc is higher than that of copper. The form of coagulant affected little the removal percentage of zinc, while addition of coagulant as the phase of solution resulted in higher removal percentage of copper than as the phase of solid.

• Horticultural Science & Technology
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• v.16 no.2
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• pp.242-243
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• 1998

This research was conducted to find the most effective and economical artificial propagation method of Lycoris squamigera among chipping, half-chipping, twin-scaling, coring, scooping and notching, in order to investigate the number of bulblets obtained from one bulb and the number of bulbs treated by a man in a day for artificial propagation. We got 42.2 bulblets from one bulb by twin-scaling, 23.2 bulblets by half-chipping, 18.2 bulblets by chipping, 14.3 bulblets by notching, 1 bulblet by coring and also 1 bulblet by scooping. Although we got the most bulblets by twin-scaling, it took the most time to treat the bulbs for artificial propagation (200 bulbs per day) and the bulblets formed were very small. But 400 bulbs were treated in a day by chipping and the bulblets formed were comparatively large. In addition, machines could be used for chipping, so chipping was considered to be the most effective and economical artificial propagation method.

• Journal of agriculture & life science
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• v.43 no.6
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• pp.29-33
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• 2009

The purpose of this study was carried out to investigate the effects of plant growth regulators on in vitro propagation of Liliem cernum Komarov. Small bulblets were poliferated from callus explants after 2 weeks and leaf, root and bulb were formed after 4 weeks culture. Leaf differentiation was promoted vigorously by the combination of TDZ 0.1 mg/L and NAA 0.01 mg/L(87.5%). The rate of root differentiation was the greatest at BA 0.2 mg/L alone(81.8%). The rate of callus formation was the high in medium containing TDZ. The number of bulblets and leaves formed in bulb scales was the greatest at TDZ 0.1 mg/L(5.7). Also, the longest length of total length, leaf and root length were in Zeatin 1.0 mg/L + NAA 0.1 mg/L(10.5 cm). However the longest bulblet was in TDZ 0.1 mg/L(1.4 cm).

• Journal of Korean Neurosurgical Society
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• v.45 no.2
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• pp.103-106
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• 2009

Intracranial schwannomas preferentially arise from the vestibular branch of the eighth nerve, and rarely from the trigeminal nerve, facial nerve, and lower cranial nerves. Anterior cranial fossa schwannomas are extremely uncommon and few details about them have been reported. The patient was a 39-year-old woman whose chief complaints were anosmia and frontal headache for 2 years. The gadolinium (Gd)-enhanced magnetic resonance imaging (MRI) showed an extra-axial mass from ethmoid sinus to right frontal base region near the midline, with solid enhancement in lower portion and multicystic formation in upper portion. The tumor was totally resected via basal subfrontal approach. At operation, the tumor had cystic portion with marginal calcification and the anterior skull base was destructed by the tumor. The olfactory bulb was involved, and the tumor capsule did not contain neoplastic cells. The histopathological diagnosis was schwannoma. We report a rare case of anterior cranial fossa schwannoma with literature review.

• Proceedings of the KAIS Fall Conference
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• 2005.05a
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• pp.269-271
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• 2005

본 연구는 수선화 구근의 각 조직으로부터 기내배양시 캘러스형성과 신초재분화를 위한 성장조절제의 효과를 측정하기 써하여 시행되었다. 신초형성과 callus의 형성은 기저부를 포함하는 floral axis에서 $50\%$의 신초발아율을 관찰하였고 scale 에서는 신초형성이 거의 관찰되지 않았다. 그리고 floral axis만을 치상한 경우는 아주 저조한 신초형성율이 관찰되었다. 지저부를 포함한 floral axis의 신초형성은 callus의 형성과 같은 NAA 0.5 mg/L과 BA 1.0 mg/L을 포함하는 MS 배지에서 만족할만한 결과를 얻었다. 신초형성으로부터 모든 신초가 형성되기까지는 약 140일이 소요되었다. 신초가 형성된 구근조직을 NAA 5.0 mg/L과 TDZ 0.02 mg/L을 포함하는 뿌리유도 MS배지에서 뿌리가 유도되는 결과를 얻었다. 본 실험에서는 수선화의 재 분화에 있어서 구근의 조직에 따라 신초형성이 다르게 나타남을 발견 할 수 있었다.

• Journal of Plant Biotechnology
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• v.40 no.2
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• pp.65-71
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• 2013

Lilium cernum Komarvo. is an important endangered plant belonging to the family Liliaceae. A method was developed for the rapid micropropagation of L. cernum through plant regeneration from bulb scales explant-derived calli. The bulb scales segments were cultured on Murashige and Skoog (MS) medium supplemented with 0, 0.5, 1.0, $3.0mg{\cdot}L^{-1}$ kinetin and 0, 0.1, 0.5, $1.0mg{\cdot}L^{-1}$ NAA or 2,4-D for callus induction. In media with $0.5{\sim}3.0mg{\cdot}L^{-1}$ kinetin and $0.1{\sim}1.0mg{\cdot}L^{-1}$ NAA and 2,4-D, 95~100% of explants produced callus. They were then transferred to MS medium supplemented with various concentrations of NAA (0, 0.01, 0.05 and $1.0mg{\cdot}L^{-1}$) in combination with BA (0, 1.0 and $2.0mg{\cdot}L^{-1}$) for bulbet formation. Bulbet induction (78%), weight (468 mg) and size (15.5 mm) were obtained the highest on MS medium containing $2.0mg{\cdot}L^{-1}$ BA and $1.0mg{\cdot}L^{-1}$ NAA. In vitro frequency of plant regeneration was not significantly treated in strength of MS and sucrose concentration. Chlorophyll contents in 1/2MS with $50g{\cdot}L^{-1}$ sucrose treatments were higher than those in control and another treatment. This in vitro propagation protocol will be useful for conservation and mass propagation of this endangered plant.

• FLOWER RESEARCH JOURNAL
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• v.17 no.1
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• pp.36-39
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• 2009

An Asiatic lily cultivar 'Apricot King' was bred in 2007 at National Horticultural Research Institute (NHRI), Rural Development Administration (RDA), Suwon, Korea. The cross was made in 2002 between Asiatic lily 'Orlando', a light orange colored cultivar, and 'Corrida', yellow and pink colored cultivar. Preliminarily selection was done as 'A04-72' in 2004. Multiplication and bulb formation, and characteristic tests were conducted from 2005 to 2006. The evaluation of this line was performed and named as 'Wongyo C1-84' in 2007 that was registered as 'Apricot King' to the registration office of Korea Seed & Variety Service. 'Apricot King' flowers at the end of June and grows average 121.6 cm. It flowers upward-facing, dark green throat and yellow orange (RHS 20A) with the size of flower 17.9 cm. Mean petal length and width is 12.8 cm and 3.7 cm, respectively. Leaves are 16.1 cm long and 2.1 cm wide. It shows light orange stigma and pollen. The weight and size of bulb is 64.2 g and 17.6 cm, respectively. For long-term storage, bulbs can be stored under -1 to $-2^{\circ}C$ for year-round forcing.

• Korean Journal of Plant Resources
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• v.31 no.4
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• pp.355-362
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• 2018

This study was performed to develop the mass propagation system using tissue culture technique to supply the seeds of Elephant garlic (Allium ampeloprasum L.) which has difficulty in propagation. Immature spathe of Elephant garlic was cultured on Murashige & Skoog (MS) medium supplemented with two plant growth regulators, naphthaleneacetic acid (NAA) and kinetin. After 6 weeks of culture, the highest number of shoot (14.9/explant) was obtained when the immature spathe with 10 cm length was cultured right after harvesting. In MS medium supplemented with 2 mg/L kinetin and 0.5 mg/L NAA, the most vigorous growth characteristics was observed, the shoot number was 14.9/explant, its length was 11.3 cm, and its fresh weight was 2.5 g. When the bulblets were cultured in MS medium with 2 mg/L kinetin and 0.5 mg/L NAA, the addition of 30 mg/L adenine improved their proliferation and growth significantly, the highest bulblet formation rate (48%) was obtained. The addition of 7% sucrose also increased the bulblet formation rate at the highest frequency of 98.2%. The shoots were shown be more vigorously proliferated at the secondary subculture stage rather than primary culture stage, their propagation rate was 80% after subculture.