• Asian-Australasian Journal of Animal Sciences
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• v.20 no.3
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• pp.393-398
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• 2007

The present study was carried out to investigate the serum minerals profile in buffaloes (Bubalus bubalis) suffering from parturient haemoglobinuria (PHU) along with minerals profile of soils and fodders from the disease prone areas and their interrelationships. Serum samples were collected from 60 each of healthy and PHU affected buffaloes randomly selected from field cases. Serum samples were collected from each animal. Fifty composite soil samples were collected where PHU was prevalent. Fifty samples of fodders including leaves and stems being fed to the diseased buffaloes were collected. The difference in the levels of calcium and potassium between upper and lower soil surface of disease prone areas under study were statistically non-significant. The mean values of phosphorous, copper, iron, selenium and molybdenum in upper soil surface were significantly (p<0.05) higher than in lower soil surface. None of the fodders offered to the diseased animals met the dietary requirements of phosphorus and copper whereas none of the fodders was deficient in potassium, iron and selenium rather were having excess of potassium, iron and selenium. The concentration of calcium was adequate in lucerne, berseem, sarson and sorghum, while maize, sugarcane and wheat straw did not meet the required levels for dairy animals. Molybdenum contents in all fodders were adequate to meet the dietary requirements of the dairy buffaloes. Serum phosphorus, copper and selenium were significantly (p<0.001) lower whereas potassium, iron and molybdenum in buffaloes suffering from PHU were significantly (p<0.001) higher than in healthy buffaloes. It was concluded that phosphorous deficient soils play a major role by transferring this deficiency to plants and ultimately reaching to animals where hypophosphataemia is a consistent finding.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.6
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• pp.876-887
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• 2015

Dicer, an ribonuclease type III type endonuclease, is the key enzyme involved in biogenesis of microRNAs (miRNAs) and small interfering RNAs (siRNAs), and thus plays a critical role in RNA interference through post transcriptional regulation of gene expression. This enzyme has not been well studied in the Indian water buffalo, an important species known for disease resistance and high milk production. In this study, the primary coding sequence (5,778 bp) of bubaline dicer (GenBank: AB969677.1) was determined and the bubaline Dicer1 biocomputationally characterized to determine the phylogenetic signature among higher eukaryotes. The evolutionary tree revealed that all the transcript variants of Dicer1 belonging to a specific species were within the same node and the sequences belonging to primates, rodents and lagomorphs, avians and reptiles formed independent clusters. The bubaline dicer1 is closely related to that of cattle and other ruminants and significantly divergent from dicer of lower species such as tapeworm, sea urchin and fruit fly. Evolutionary divergence analysis conducted using MEGA6 software indicated that dicer has undergone purifying selection over the time. Seventeen divergent sequences, representing each of the families/taxa were selected to study the specific regions of positive vis-$\grave{a}$-vis negative selection using different models like single likelihood ancestor counting, fixed effects likelihood, and random effects likelihood of Datamonkey server. Comparative analysis of the domain structure revealed that Dicer1 is conserved across mammalian species while variation both in terms of length of Dicer enzyme and presence or absence of domain is evident in the lower organisms.

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.10
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• pp.1406-1415
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• 2013

The study was conducted to correlate the periparturient immune status in terms of neutrophil functions and cytokine expression in peripheral blood mononuclear cell culture with impending postpartum reproductive disorders in buffaloes. Forty pregnant buffaloes were observed for occurrence of postpartum reproductive disorders (PRD), i.e., metritis, endometritis and delayed uterine involution etc., during one week prepartum to four weeks postpartum period. A representative number (n = 6) of buffaloes that did not develop any PRD were included in group I (healthy, control), while the animals which experienced PRD were assigned into group II (PRD, n = 8). The blood samples were collected at weekly interval from one week prepartum to four weeks postpartum period considering the day of calving as 'd 0'. Differential leucocytes counts, superoxide and hydrogen peroxide production activity in isolated neutrophils and the mRNA expression profile of cytokines i.e., IL-2, IL-4 and IFN-${\gamma}$ in PBMC culture were studied in all the samples. A higher total leucocytes, neutrophil and band cells count along with impaired neutrophil functions i.e., lowered level of production of superoxide and hydrogen peroxide before parturition and during early postpartum period were observed in buffaloes developing PRD. Further, a lower expression of IL-2, IFN-${\gamma}$ and IL-4 mRNA in PBMC culture was observed at calving in buffaloes that subsequently developed PRD at later postpartum. Thus, suppression in neutrophil function and cytokine expression at prepartum to early postpartum period predisposes the buffaloes to develop postpartum reproductive disorders. Hence, monitoring of neutrophils function and cytokine expression profile would be effective to predict certain reproductive disorders at late pregnancy or immediately after parturition in buffaloes. In future, this may be a novel approach for determining suitable management and therapeutic decisions for prevention of commonly occurring reproductive disorders in farm animals.

• Journal of the Korean association of regional geographers
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• v.13 no.3
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• pp.290-300
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• 2007

In this study, web document frequency of city place name is analyzed and it is used as the dataset for rank-size analysis. The search keywords are compared in the context of spatial meaning and the different domain corpus is applied. The acquired search results are applied for the further analysis. Firstly, the rank-size analysis is applied to compare the result between population and document frequency. Secondly, in case of correlation analysis, the significant changes are revealed when the spatial criteria for search keywords are increased. In case of corpus, COM, NET, and ORG shows the higher coefficient values. Lastly, the cluster analysis is applied to classify the list of cities that shows the similarity and difference. These analyses have a significant role in representing the rank-size distribution of city names that are reflected on the web documents in the information society.

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.3
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• pp.313-316
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• 2004

Ultrasonographic studies were conducted on eight Murrah buffaloes daily from day 6 postpartum (pp) onwards till day 77 pp to monitor changes in the cervix, uterine horn and ovarian follicular growth and development. The mean size of horn and cervix on day six ($9.07{\pm}0.74$ and $8.58{\pm}0.00cm$) decreased significantly to $4.09{\pm}0.09$ and $3.56{\pm}0.08cm$ by day 27 pp, respectively. Follicles in 50% of the buffaloes ovulated within 24 to 54 days pp and the size of the largest follicle on different days increased to more than 5 mm. The remaining 50 percent of animals ovulated after 65 days postpartum. Large size follicles (>8.5 mm) appeared in six out of eight buffaloes between 10 to 30 days pp and five animals had ovulated during early postpartum period. Waves pattern of follicular growth was observed during early postpartum period. Ovulatory follicles growth rate was more than the anovulatory follicles and increase in size was more as compared to the subordinate follicle. Anovulatory follicles persisted for longer period. Mean size of large follicle was more from day 6 to 41 pp and again from 50 to 65 pp in cyclic animals. Second large follicle were large during early postpartum (18days), thereafter, its size was more in acyclic animals. Small follicles population was less in cyclic animals upto day 50 postpartum. Mean medium size follicle growth pattern did not differ in cyclic and acyclic groups. Large size follicle number was more in cyclic group (5/8) during 14 to 20 days postpartum. Presence of large follicles (>8.5 mm) showed initiation of ovarian activity.

• Journal of the Korean Society of Clothing and Textiles
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• v.35 no.9
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• pp.1112-1124
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• 2011

The development of computer graphics and Internet technology has created a 3D Web-based virtual world that has transformed the global fashion industry environment. In this study, the application cases of 3D virtual fashion education were analyzed to discuss the necessity and application of a 3D apparel CAD curriculum as part of a special education for global fashion talent at Korean fashion-related colleges. Prior studies, literature, photo data and Internet data (in and out of Korea) were used for this study. The demonstration case studies were conducted for the virtual fashion education 'SLCC 2007' of Buffalo State College (U.S.), 'Ratava's Line (2004)' of SFU/FIT Collaborative Design Project (Canada and U.S.) and '2011 Graduation Fashion Show' of Ueda Fashion College (Japan). The results of the study show that the 3D apparel CAD system (as a core technology of the IT fashion industry) would allow the current mass production concept to change to a new paradigm of 'mass customization' along with new fashion business types that include global fashion companies and Web-based Internet, mobile and virtual-world shopping malls. In addition, it appears that the system should be included in the curriculum of fashion-related colleges and institutes to educate technical designers for the global fashion industry and global fashion talent with comprehensive system operation and management ability, and to promote single proprietor companies.

• Asian Journal of Innovation and Policy
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• v.9 no.2
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• pp.187-206
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• 2020

Under the on-going evolution of the COVID-19 pandemic, estimating the economic impact of the pandemic is highly uncertain and challenging. This situation makes it difficult for policymakers, governors, and economic entities to formulate appropriate responses and decision makings. To provide useful information about the effect of the COVID-19 pandemic on the Korean economy, this study examined macroeconomic impact analysis stemming from the pandemic shocks with different scenarios for the Korean economy. Based on three scenarios using the growth rate of 2020 GDP and consumer expenditure patterns, the 2021 GDP by industry sector was forecast with two new approaches. First, the recovering process of the Korean economy from the shock was analyzed by applying a Flex-IO method. Second, a new forecasting approach combined with an IO coefficient matrix was applied to forecast the future GDP changes. The findings of this study are summarized as follows: First, the total GDP growth rate under the Pessimistic Scenario demonstrates less rebound from the shock than that of the Base Scenario. Second, agriculture, culture, and tourism-related sectors that are suffering from the severe losses of COVID-19 showed lower resilience than other different industries. Third, information and communications technology (ICT) industry maintains a stable growth trend and is expected to take the leading role for the Korean economy in the post-COVID-19 and the Industry 4.0 eras. The findings deliver that it needs to analyze how government expenditure responding the shock into the forecasting model, which can be more useful and reliable to simulate the resilience from the pandemic.

• Proceedings of the Microbiological Society of Korea Conference
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• 2008.05a
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• pp.171-171
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• 2008

We monitored the occurrence of human enteric viruses in urban rivers by cell culture-PCR and RT-nested PCR. Water samples were collected monthly or semimonthly between May 2002 and March 2003 in four urban tributaries. Enteric viruses were detected by RT-nested PCR and cell culture-PCR based on a combination of Buffalo Green monkey kidney (BGMK) and A549 cell lines, followed by phylogenetic analysis of amplicons. By RT-nested PCR analysis, 45 (77.6%), 32 (55.2%), 32 (55.2%), 26 (44.8%), 12 (20.7%), 2 (3.4%), 4 (6.9%), and 4 (6.9%) of 58 samples showed positive results with adenoviruses, enteroviruses, noroviruses (NV) genogroup I (GI) and II (GII), reoviruses, hepatitis A viruses, rotaviruses and sapoviruses, respectively. Adenoviruses were most often detected and only eight (13.8%) samples were negative for adenoviruses and positive for other enteric viruses in the studied sites. Thirty-one (77.5%) of the 40 samples were positive for infectious adenoviruses and/or enteroviruses based on cell culture-PCR, and the frequency of positive samples grown on A549 and BGMK (65.0%) was higher than that grown on BGMK alone (47.5%). The occurrence of each enteric virus, except reoviruses and hepatitis A viruses was not statistically correlated with the water temperature and levels of fecal coliforms according to Binary logistic regression model. By sequence analysis, most strains of adenoviruses and enteroviruses detected in this study are similar to the causative agent of viral diseases in Korea and most NV GI- and GII-grouped strains were closely related to the reference strains from China and Japan, and GII/4-related strains had similar sequences to strains recognized as a worldwide epidemic outbreak. Our results suggested that monitoring human enteric viruses is necessary to improve microbial quality and cell culture-PCR using the combination of A549 and BGMK cells and the adenovirus detection by PCR could be useful for monitoring viral contamination in the aquatic environment.

• Food Science of Animal Resources
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• v.37 no.1
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• pp.18-28
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• 2017

Pastirma is a dry-cured meat product, produced from whole beef or water buffalo muscles. This study was carried out to investigate the effect of production stages (raw meat, after curing, after $2^{nd}$ drying and pastirma) on the total lipid, neutral lipid, phospholipid and fatty acid composition of phospholipid fraction of pastirma produced from beef M. Longissimus dorsi muscles. The pH and colour ($L^*$, $a^*$ and $b^*$) analyses were also performed in raw meat and pastirma. It was found that pastirma production stages had significant effects (p<0.01) on the total amounts of lipid, neutral lipid and phospholipid, and the highest amounts of lipid, neutral lipid and phospholipid were detected in pastirma. In pastirma, neutral lipid ratio was determined as $79.33{\pm}2.06%$ and phospholipid ratio as $20.67{\pm}2.06%$. Phospholipids was proportionately lower in pastirma than raw meat. Pastirma production stages affected pentadecanoic acid (15:1) (p<0.01), linoleic acid (18:2n-6) (p<0.05), ${\gamma}-linoleic$ acid (18:3n-6) (p<0.05), erucic acid (22:1n-9) (p<0.05), docosapentaenoic acid (22:5n-6) (p<0.05), total unsaturated fatty acid (${\Sigma}USFA$) (p<0.05) and total saturated fatty acid (${\Sigma}SFA$) (p<0.05) ratios of phospholipid fraction and also the moisture content (p<0.01). Pastirma process also affected pH and colour ($L^*$, $a^*$ and $b^*$) values (p<0.01), and these values were higher in pastirma than raw meat.

• Wind and Structures
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• v.20 no.5
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• pp.701-718
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• 2015

The joint distribution of wind speed and wind direction at a bridge site is vital to the estimation of the basic wind speed, and hence to the wind-induced vibration analysis of long-span bridges. Instead of the conventional way relying on the weather stations, this study proposed an alternate approach to obtain the original records of wind speed and the corresponding directions based on field measurement supported by the Structural Health Monitoring System (SHMS). Specifically, SHMS of Sutong Cable-stayed Bridge (SCB) is utilized to study the basic wind speed with directional information. Four anemometers are installed in the SHMS of SCB: upstream and downstream of the main deck center, top of the north and south tower respectively. Using the recorded wind data from SHMS, the joint distribution of wind speed and direction is investigated based on statistical methods, and then the basic wind speeds in 10-year and 100-year recurrence intervals at these four key positions are calculated. Analytical results verify the reliability of the recorded wind data from SHMS, and indicate that the joint probability model for the extreme wind speed at SCB site fits well with the Weibull model. It is shown that the calculated basic wind speed is reduced by considering the influence of wind direction. Compared to the design basic wind speed in the Specification of China, basic wind speed considering the influence of direction or not is much smaller, indicating a high safety coefficient in the design of SCB. The results obtained in this study can provide not only references for further wind-resistance research of SCB, but also improve the understanding of the safety coefficient for wind-resistance design of other engineering structures in the similar area.