• Proceedings of the Microbiological Society of Korea Conference
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• 2009.05a
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• pp.129-130
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• 2009

• Proceedings of the PSK Conference
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• 2002.04a
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• pp.74-75
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• 2002

• Proceedings of the Korean Society for Bioinformatics Conference
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• 2005.09a
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• pp.278-283
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• 2005

Cell cycle is regulated cooperatively by several genes. The dynamic regulatory mechanism of protein interaction network of cell cycle will be presented taking the budding yeast as a sample system. Based on the mathematical model developed by Chen et at. (MBC, 11,369), at first, the dynamic role of the feedback loops is investigated. Secondly, using a bifurcation diagram, dynamic analysis of the cell cycle regulation is illustrated. The bifurcation diagram is a kind of ‘dynamic road map’ with stable and unstable solutions. On the map, a stable solution denotes a ‘road’ attracting the state and an unstable solution ‘a repelling road’ The ‘START’ transition, the initiation of the cell cycle, occurs at the point where the dynamic road changes from a fixed point to an oscillatory solution. The 'FINISH' transition, the completion of a cell cycle, is returning back to the initial state. The bifurcation analysis for the mutants could be used uncovering the role of proteins in the cell cycle regulation network.

• Journal of Microbiology and Biotechnology
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• v.10 no.5
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• pp.606-611
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• 2000

Dimorphic yeast Candida albicans reversibly switches between the form of yeast and hyphae depending on external conditions. We investigated possible roles of the myosin family in the growth and dimorphic switches of C. albicans with a general myosin ATPase inhibitor, 2,3-butanedione-2-monoxime (BDM). Transition to hyphae as well as proliferation by budding was completely inhibited by BDM at 16 mM. Presence of 16 mM BDM did not affect hyphae-to-bud transition but it blocked budding. The effects of BDM on yeast growth and dimorphic switches were reversible. More than 70% of the BDM-treated cells demonstrated defects in the amount and the polarized localization of F-actin as well as in the shape and migration of the nucleus, suggesting that myosin activities are needed in these cellular processes of C. albicans.

• Journal of Microbiology and Biotechnology
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• v.27 no.6
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• pp.1198-1203
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• 2017

Hrr25, a casein kinase $1{\delta}/{\varepsilon}$ homolog in budding yeast, is essential to set up mono-orientation of sister kinetochores during meiosis. Hrr25 kinase activity coordinates sister chromatid cohesion via cohesin phosphorylation. Here, we investigated the prophase role of Hrr25 using the auxin-inducible degron system and by ectopic expression of Hrr25 during yeast meiosis. Hrr25 mediates nuclear division in meiosis I but does not affect DNA replication. We also found that initiation of meiotic double-strand breaks as well as joint molecule formation were normal in HRR25-deficient cells. Thus, Hrr25 is essential for termination of meiotic division but not homologous recombination.

• Proceedings of the Korean Society for Bioinformatics Conference
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• 2004.11a
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• pp.50-56
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• 2004

Bifurcation analysis of cell cycle regulation in the budding yeast is performed basedon the mathematical model by Chen et al [Molecular biology of cell, 11:369-391, 2000]. On the bifurcation diagram, locations of both stable and unstable solutions of the nonlinear differential equations are presented by taking the mass of cell as a controlparameter. Based on the bifurcation diagram, dynamic mechanism underlying the 'start' transition, initiation of a new round of cell cycle, and the 'finish' transition, completion of cell cycle and returning back to the initial state, is discussed: the 'start' transition is a transition from a stable fixed solution for a small mass and to an oscillatory state for a large mass, and the 'finish' transition is a switching back to the stable fixed solution from the oscillatory state. To understand the role of the genes during the cell cycle regulation, bifurcation diagrams for the mutants are compared with that of the wild type.

• Journal of Sericultural and Entomological Science
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• v.44 no.2
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• pp.56-58
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• 2002

An autotetraploid, Sawonppong 28 was derived from a thick-leaf and highly productive mulberry variety, Huyeoprok, by colchicine solution treatment on growing point and its characteristics were as follows. The number of somatic chromosomes of the derived variety was 2n=56 of an autotetraploid and green tip sprouting stage was medium budding variety with triangular oval shape and thick leaves contained rich chlorophyll. The length of branches was comparatively short and the number of branches was also small. However the branches were thick and non-budding length percentage at basal part of branch was high and cold hardiness was weak.

• Proceedings of the Microbiological Society of Korea Conference
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• 2005.05a
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• pp.93-93
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• 2005

• Journal of Microbiology and Biotechnology
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• v.18 no.6
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• pp.1059-1063
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• 2008

Abnormal phenotypes resulting from haploinsufficiency (HI) are due to the loss of one allele. Recent studies in budding yeast have shown that HI originates from insufficient protein levels or from a stoichiometric imbalance between subunits of protein complexes. In humans, however, HI often involves transcription factors. Therefore, the species differences in HI and the molecular mechanisms of species-specific HI remain under investigation. In this study, HI in fission yeast was systematically surveyed. HI in fission yeast affected genes related to signaling and to basic cellular processes, as observed in budding yeast. These results suggest that there are species differences in HI and that the HI that occurs in fission yeast is intermediate to HI in budding yeast and humans.

• Proceedings of KOSOMES biannual meeting
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• 2004.11a
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• pp.129-135
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• 2004

Plate has cutout inner bottom and girder and floor etc in hull construction absence is used much, and this is strength in case must be situated, but establish in region that high stress interacts sometimes fatally in region that there is no big problem usually by purpose of weight reduction, a person and change of freight, piping etc. Because cutout's existence gnaws in this place, and, elastic budding strength by load rouses large effect in ultimate strength. Therefore, perforated plate elastic budding strength and ultimate strength is one of important design criteria which must examine when decide structural elements size at early structure design step if ship. Therefore, and, reasonable elastic budding strength about perforated plate need design ultimate strength. Calculated ultimate strength change several aspect ratioes and cutout's dimension, and thickness in this investigation. Used program applied ANSYS F.E.M code based on finite element method