• Journal of Bio-Environment Control
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• v.20 no.4
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• pp.382-386
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• 2011

This study aims to identify the cause for low appearance of flowers in 'Heukgoosul' grapevines, and determine basic characteristics of the axillary buds and types of necrosis in order to find out their influence on necrosis. As for $1^{st}$ to $4^{th}$ node, the closer buds were located to cane, the smaller the size of buds became. However, there was no difference in the size of an axillary bud in the upper part of the shoot above the 4th node. The bud necrosis occurrence in the $1^{st}$ node was the highest with 32% while buds in $4^{th}$ to $10^{th}$ node were normal with 84~96%. The size of buds in July was the largest with 6.40 mm, while buds showed no difference from August to October. The bud necrosis and main bud necrosis occurred most frequently in October, however accessory bud necrosis took place from July demonstrating no difference since then. The analysis on the relationship between shoot vigor and necrosis of axillary buds showed that the shoot diameter and internodes' length have no co-relationship with axillary bud necrosis, but there were negative relationship between the size of buds and necrosis occurrence, which was the most related to accessory bud necrosis. Therefore, despite the low occurrence of bud necrosis and healthy buds in 'Heukgoosul' grapevines, there was little appearance of flowers in the grapevines, which was attributable to the necrosis in the first bud. In conclusion, the research suggests spur pruning with three nodes kept intact yield.

• Korean Journal of Animal Reproduction
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• v.16 no.4
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• pp.387-397
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• 1993

To examine local response of adenosie(purine nucleoside) on the developing mammary gland, Elvax 40P implants containing adenosine were surgically implanted into mammary fat pad of the five week old female ICR mice. Inguinal(the 4th) mammary glands of anesthetized mice were exposed andplaced the implants for 12 days. One gland was treated with an adenosine implant, while the contralateral gland received a blank implant as control. For whole-mount preparations, glands were stained with alum carmine, and for histological observation, micro-selected mammary glands were stained with hematoxylin and eosin Y. Implantation with Elvax 40P did not affect on the damage of neighboring mammary tissue. Adenosie 25 or 250$\mu\textrm{g}$ per slow-release implant stimulated local mammary end bud formation of ovariectomized mice such as end bud size and numbers of end bud per gland in a dose dependent manner(P<0.05), and lower concentration of adenosie(2.5 or 25$\mu\textrm{g}$/implant) increased numbers of end bud(P<0.05) and end bud size(P<0.1) of intact mice. Adenosine treatment and intact ovarian function had moderate interation effects on the stimulation of end bud formation at 2.5$\mu\textrm{g}$ adenosine/implant(P<0.1). In histological observation, adenosine implants increased numbers of mammary epithelial type of cells at mammary duct in the presence or absece ofovary. These results indicate that adenosine should be one of regulators in mouse mammary ductal growth.

• Horticultural Science & Technology
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• v.29 no.5
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• pp.407-412
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• 2011

The germination of the buds in 'Hongisul' grapes is poor and especially the high ratio of shoots where the flowers failed to emerge it was difficult to secure a sufficient amount of harvest. Thus, in this study, the necrosis and the periodical change in the buds were observed morphologically and also the cause of necrosis of the bud was investigated in order to understand what was causing the low germination and flowering. There was no change in the size of the bud after June and based upon external observation, there wasn't any retrogression or withering. However, based on a microscopic examination, the ratio of whole bud and main bud necrosis of the 'Hongisul' grapes continued to increase after August, and specially the ratio of the main bud necrosis continued to increase up to October. As for the size of the buds on the shoots, the buds located on the $1^{st}-3^{rd}$ nodes from the basal part were small whereas the buds located on the $4-10^{th}$ nodes were comparatively larger in its size. The ratio of necrosis of the bud was the highest at the $1^{st}$ and $2^{nd}$ bud, meaning that the buds located at the basal part of the shoot were defective compared to those located at the end of the shoot. It was also found that when the growth of the shoot is active and the shoot diameter is thick, it hindered the development of the buds. Therefore, it was judged that long pruning of 'Hongisul' grapes would help the emergence of the flowers as you could use the buds that have comparatively developed better.

• Korean Journal of Agricultural Science
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• v.47 no.4
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• pp.987-993
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• 2020

This study investigated the effective harvest of 'Kyoho' grapevines by examining their characteristics including bud development, necrosis types and flower primordium formation. The size of the axillary bud did not show any difference in the 5th node or more, but it was smaller because it was closer to the base in the 4th node or less. In the 1st node, the rates of main bud necrosis (MBN), accessory bud necrosis (ABN), and whole bud necrosis (WBN) were high, and the rate of flower primordium formation was low, but there was no significant difference in the other nodes. Therefore, it was expected that using other nodes than the 1st node would be advantageous to secure production. Because the growth progresses after sprouting, the main bud necrosis rate increases, showing a very low flower primordium formation rate in March of the following year. Therefore, a method is needed to increase the storage nutrients in the winter and the rate of flower primordium formation after March. This study found that the thickness of the shoots should be less than 8.5 mm between the 3rd and 4th nodes, and the length should be less than 60 cm for nodes up to the 10th node.

• Korean Journal of Plant Resources
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• v.8 no.3
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• pp.265-274
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• 1995

Characteristics of vegetative spur leaf, spur bud, fruiting supr leaf and flowering in relation to canopy position was investigated on order to promote better spur bud and flower bud differentiation on Citrus junos. There was no difference in vegetative spur leaf characteristics among directions. Vegetative spur leaf number and leaf area showed no difference between top and bottom position. There was no difference in spur bud size among directions, but spur bud at top was larger than that at bottom. Specific leaf weight of vegetative spur was mostly influenced by light interception, and leaf dry weight per spur average leaf dry weight, spur bud length and diameter had also a very high correlation with light interception rate. Shading and GA treatment in spur bud and flower bud differentiation was ineffective than natural light. Fruit thinning enhanced fruit quality as well as flower bud differentiation through an increase of leaf number per fruit, over 40 in Citrus junos.

• Korean Journal of Weed Science
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• v.9 no.1
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• pp.28-33
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• 1989

This study was examined for the effect of tuber size, bud and tuber removal, and tuber cutting on growth of Eleockaris kuroguwai Ohwi. Elongation of plumule and radicle, and the weights of tuber, rhizome and shoot were increased as tuber size is large. The weights of shoot and tuber reduced by bud planting without tuber at 15 days after budding, however number of tubers were increased compared with normal tuber. When cross-cutting tubers were seeded, the weights of shoot, rhizome and tuber were produced as 84%, 85%, 71%, respectively compared with normal tuber, and the number of tubers was not influenced. The weights of tuber, rhizome and shoot were lowed as the bud removal, and there were a great number of tubers at bud removal of 15 days after budding. Tuber of E. k, contained as nonfibrous 78.9gr, phosphorous 77mg, iron 16.8mg, niacin 1.3mg per 100gr edble portion. The positive correlations were observed between shoot weight and tuber weight, and number of tubers.

• Korean Journal of Plant Resources
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• v.15 no.1
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• pp.8-17
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• 2002

To improve new propagation method, I try to propagate wild polygonatum odoratum roots which formate adventitious bud without bud. The obtained results of size of roots and adventitious bud to formate for multiple propagation of wild polygonatum odoratum as the follow. One year grown wild polygonatum odoratum root in 5cm diameter formate 66.5％ adventitious buds. One year grown wild polygonatum odoratum root in 15cm in diameter germinate 113% adventitious bud. Two years grown root in 15cm diameter formate adventitious bud three times more then one year root. Wild polygonatum odoratum in 15cm diameter without any bud in experiment plot makes 4 times of propagation. Sand and Perlite media propagaton plot was increased 3.1∼3.3times more than common culture medium. The best result of mass propagation wild polygonatum odoratum is 10mm diameter root and 5cm cut in length to plant sand media plot.

• Tuberculosis and Respiratory Diseases
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• v.85 no.1
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• pp.25-36
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• 2022

Background: Only a few studies directly compared the therapeutic efficacy and safety of two pressurized metered-dose inhalers (pMDIs) in asthma. We analyzed the asthma treatment outcomes, safety, and patient preferences using formoterol/beclomethasone (FORM/BDP), a pMDI with extra-fine particles, compared with formoterol/budesonide (FORM/BUD), another pMDI with non-extra-fine particles. Methods: In this randomized, double-blind, double-dummy parallel group study, 40 adult asthmatics were randomized to FORM/BDP group (n=18; active FORM/BDP and placebo FORM/BUD) or FORM/BUD group (n=22; active FORM/BUD and placebo FORM/BDP). During the two visits (baseline and end of 8-week treatment), subjects were asked to answer questionnaires including asthma control test (ACT), asthma control questionnaires (ACQ), and Quality of Life Questionnaire for Adult Korean Asthmatics (QLQAKA). Lung function, compliance with inhaler, and inhaler-handling skills were also assessed. Results: Ten subjects in the FORM/BDP group and 14 in the FORM/BUD group completed follow-up visits. ACT, ACQ, QLQAKA (a primary outcome), and adverse events did not differ between two groups. We found that the increase in forced expiratory volume in 1 second/forced vital capacity and forced expiratory flow at 25% to 75% of the pulmonary volume in the FORM/BDP group was higher than in the FORM/BUD group. Regarding preference, subjects responded that the flume velocity of FORM/BDP was higher, but more adequate than that of FORM/BUD. They also answered that FORM/BDP reached the trachea and bronchus and irritated them significantly more than FORM/BUD. Conclusion: The use of pMDI with extra-fine particles may relieve small airway obstruction more than the one with non-extra-fine particles despite no significant differences in overall treatment outcomes. Some asthmatics have a misconception about the adequacy of high flume velocity of pMDIs.

• The Korean Journal of Ecology
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• v.14 no.3
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• pp.305-316
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• 1991

Growth hadit and demograph in a mature population of polygonatum and polygonatum humile in kanghwa island were studied for two growing seasons. Shoots of two species emerged early spring from the growing apices of the underdground rhizomes which had persisted for up to 1~5 year.after flowering, the ramets produced two rhizome buds at the shoot base. Bacause of the apical dominance in the rhizome system, a new rhizome was developed from only one bud, an actual bud,and the other latent buds were suppressed sothat remained dormant. The latent dud produced a new rhizome only when the actual dud was severed by the herbivores or by the physical obstacles. Therefore, the ramet number is not increased by the new rhizome from the latent bud. however, new ramets dould sometimes grow from latent buds which had been produced more than a year ago. Production of these ramets was main means increasing the ramet numbew and widening the potential zone of exploitation. Changes in size class of each ramet were noyiceable after a tear in small size-classes. Small ramets replaced themselves with larger-sized ramet, while large ramets with similar-sized or smallar-sized ramets. ramet numbers were average 0.82and 1.14 times of those fromthe previous year inp. involucratum and p. humile, though there was between-site variation. Almost all the ramets in the quadrats were alive during the growing season. when the entire rhizome systems were excavater next spring, there were many rhizomesegments without shoots, especially in p. involucratum. therefore, the drcrease of ramet number in p. involucratum in probably due to the climatic factors of winter.

• Journal of the korean academy of Pediatric Dentistry
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• v.24 no.3
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• pp.688-703
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• 1997

The effect of glossopharyngeal nerve transection on the taste buds of the rat vallate papilla was examined by using the method of DNA nick-end labeling (TUNEL) and standard electron microscopic technique at 1, 3, 5, 7, 9 days after denervation. In general, the number and size of taste buds decreased as more days passed after denervation. They started decreasing on day 3 post denervation and virtually all taste buds were disappeared on day 9 post denervation. In studies using TUNEL method, TUNEL postive cells markedly increased in their numbers one day post denervation, as compared with controls. The number of apoptotic taste bud cells per taste bud profile was averaged to be 0.64 and 0.44 for day 1 and 3 post denervation, respectively, whereas it was 0.10 in controls. In electron microscopy, apoptotic cells were identified by the presence of condensed and fragmentary nuclei in a cytoplasm, which resulted in increased density. In control rats, only few apoptotic cells were found. On days 1 and 3 post denervation, nerve fibers almost disappeared from the taste buds and some apoptotic cells were apparent. On days 7 and 9 post denervation, a few taste bud cells were still present in the epithelium of the bottom of the trench wall of the vallate papilla and most of them showed apoptotic changes. The results indicate that the death of taste bud cells in normal conditions is controlled by apoptosis and the decrease and disappearance of taste buds after denervation is also caused by apoptosis of taste bud cells.