• Korean Journal of Poultry Science
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• v.31 no.4
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• pp.283-291
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• 2004

Feeding trials were conducted with Euglena strains grown under different media. The effect of supplementation of Euglena on the laying performance, egg quality and fatty acid composition of egg yolk was studied. In experiment I, two hundred eighty 32-wk-old ISA Brown layers were randomly assigned to seven dietary treatments for 4 wks. Each treatment consisted of 4 replications with 10 birds each housed in two birds cages. Control diet was formulated to have $17\%$ CP and 2,750 kcal ME/kg. Euglena gracilis Z. (EG) was added to control diet at the level of 0.25, 0.5, $1.0\%$ and Euglena gracilis Z. bleached and DHA enriched (EGBD; a strain mutated by streptomycin and cultivated in DHA enriched medium) at the level of 0.5, 1.0, $2.0\%$ in the diet. In experiment 2, three hundred 84-wk-old ISA brown layers were randomly assigned to five dietary treatments: T1; Control, T2; T1 + EGBD $0.5\%$, T3; T1 + Euglena gracilis Z. DHA enriched (EGD; cultivated in DHA enriched medium) $0.5\%$, T4; T1 + EGD $1.0\%$, T5; T1 + EGD $2.0\%$. Each treatment had 5 replication of 12 birds each housed in two birds cages. In experiments 1 and 2, Euglena suppplementation did not significantly affect egg production but increased egg weight and feed intake. In experiment 1, EG was more effective in increasing egg yolk color score than EGBD. Egg yolk color of EG $1\%$ treatment showed the highest score. EGBD supplementation increased DHA concentration of egg yolk. EGBD $2\%$ treatment showed the highest DHA and the lowest palmitic and stearic acids concentration in the egg yolk. In experiment 2, EGBD $0.5\%$ treatment showed highest DHA level in egg yolk (P<0.05). It was conducted that EGBD is a single cell protein source rich in DHA, that can be used to produce DHA enriched eggs.

• Korean Journal of Poultry Science
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• v.36 no.2
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• pp.103-110
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• 2009

This experiment was conducted using 350 Lohmann Brown layers (67 weeks of age) to evaluate the dietary effect of organic or inorganic Se and Cu on their contents in chicken eggs. The layers were divided into seven groups and fed a commercial diet or one of the six experimental diets containing different levels of Se and Cu (T1, 0.3ppm organic Se; T2, 1.0ppm organic Se; T3, 1.0ppm inorganic Se; T4, 125ppm organic Cu; T5, 250ppm organic Cu; and T6, 250ppm inorganic Cu) for 5 weeks. No significant differences were observed in egg production and its qualities among groups. The contents of blood cholesterol in the birds fed various levels of Se and Cu were not significantly different as compared to control. Se contents in eggs were linearly increased as dietary Se levels increased for both sources, but Se contents from the groups fed organic Se were slightly higher than those fed inorganic Se. Sensory characteristics of eggs were not influenced by dietary treatments. In conclusion, Se contents in eggs were linearly increased as dietary levels of organic or inorganic Se increased, but eggs in layers fed the diet containing organic Se did not show higher Se contents than those in birds fed dietary inorganic Se.

• Journal of Animal Environmental Science
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• v.18 no.3
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• pp.183-190
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• 2012

The objective of this study was to evaluate the effects of an unexpected change in housing environment on stress in poultry. Laying hens (Hy-line Brown), adapted to a free-range housing system for more than a month, were randomly divided into two groups to be subjected to an unexpected change of their housing environment: one half of them were individually housed into conventional battery cages and the other continually left in the same housing system throughout the entire period of the study. The sudden change resulted in an increase in egg production and albumen height (P<0.05), but decrease in eggshell thickness (P<0.05). The change had tendency to increase Haugh unit ($P{\leq}0.061$) and to decrease eggshell color ($P{\leq}0.074$), but did not affect body weight for 5 days thereafter. No significant changes were detected in liver color. Plasma concentrations of corticosterone was acutely increased one day after the change (P<0.05), then returned to similar control levels. The results of the present study indicate that unexpected changes in housing environment cause acute increase in stress hormone concentrations, but interpretation of the results should be cautious due to the experimental conditions.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.7
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• pp.1019-1025
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• 2006

Two experiments were conducted separately to study the effect of astaxanthin on production performance and egg quality in laying hens and meat quality in finishing pigs. In Experiment 1, four hundred Brown Hy-Line layers, 26 weeks of age, were randomly divided into five treatments according to a single factorial arrangement. Each treatment had four replicates comprising 20 birds each. The dietary treatments were: 0, 0.7, 0.9, 1.1 and 1.3 ppm of astaxanthin fed for 14 days. Then all the birds were fed an astaxanthin-free diet (0 ppm astaxanthin) for an additional 7 days. The results showed that dietary astaxanthin had no significant effect on layer production performance. There was no significant effect (p>0.05) on egg weight, yolk height and Haugh unit (HU) with increasing dietary astaxanthin level and increased storage time. Yolk color was linearly increased (p<0.01) with the increasing dietary astaxanthin level and significantly decreased with the increasing storage time (p<0.05). The TBARS value in yolk decreased linearly (p<0.05) with increasing amount of dietary astaxanthin and storage time. When the diets were replaced with the astaxanthin-free feeds, all parameters concerning egg quality decreased with increasing days of measurement, especially the yolk color, and HU significantly decreased (p<0.05). In experiment 2, thirty-six barrows ($L{\times}Y{\times}D$), $107{\pm}3.1kg$ BW, were randomly divided into three treatments according to a single factorial arrangement. Each treatment had three replicates comprising 4 pigs each. The dietary treatments were: 0, 1.5 and 3.0 ppm of astaxanthin fed for 14 days. The results showed that dietary astaxanthin had no significant effects on production performance. There was a linear effect (p<0.05) on dressing percentage, backf.at thickness and loin muscle area with increasing dietary astaxanthin level. There were no significant effects (p>0.05) on the TBARS value, drip loss, meat color, marbling and $L^*$, $a^*$, $b^*$ values. Cholesterol concentration in meat was not affected by dietary addition of astaxanthin. It could be concluded that astaxanthin supplementation was beneficial to improve egg yolk color; egg quality during storage and it also could improve the meat quality of finishing pigs.

• Journal of Animal Science and Technology
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• v.45 no.5
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• pp.847-856
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• 2003

This study was carried out to investigate the effects of dietary xanthophylls supplementation on pigmentation and antioxidant properties in the egg yolk. ISA Brown laying hens aged 50 weeks were fed five kinds of xanthophyll combination such as control(neither natural nor artificial xanthophylls in feed), T1(Commercial diet containing natural xanthopylls in grain + Lutein 10ppm + Capsantin 10ppm), T2(T1 + Capsanthin 65ppm), T3(T1 + Canthaxanthin 65ppm), T4(T1 + Capsanthin 10ppm + Canthaxanthin 65ppm), and T5(T1 + Capsanthin 65ppm + Canthaxanthin 10ppm). The pH values of all egg yolks were not significantly different during storage or feeding periods. CIE L$^{*}$(lightness) values of T2 ~ T5 egg yolks were lower than those of control and T1. Conversely, the CIE a$^{*}$(redness) value of T2 ~ T5 egg yolks showed significantly higher(P<0.05). Egg yolk from chicks fed xanthophylls increased CIE b$^{*}$ values. The CIE b$^{*}$(yellowness) values of T2 ~ T5 egg yolks were higher than control and T1 during storage at 37$^{\circ}C$ for 48 hours respectively. In the antioxidation experiment, dietary xanthophylls supplementation affected lipid antioxidation of egg yolk during storage. The TBARS(O.D.) of egg yolks from chicks fed xanthophylls were lower than that of control during incubation at 37$^{\circ}C$ for 10 hours. In conclusion, dietary xanthophylls supplementation influence to color difference and retardation of lipid oxidation in egg yolk.

• Journal of the Korea Organic Resources Recycling Association
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• v.11 no.1
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• pp.102-112
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• 2003

The objective of this study was to investigate the effects of food waste and probiotics supplementation on productive performance and egg composition of laying hens. Total 120 layers (Brown Tetran) 22 weeks old were divided into five groups with 4 replicates for each treatments and raised in battery cage. The experimental design was completely randomized. The egg Production rate was significantly increased in layer supplemented with 0%, 0.5%, 1.0% and 1.5% of probiotic supplemented diets compared to control (P<0.05). At feeding with food waste and probiotic supplementation had no effect on the egg shell strength (P>0.05). Haugh unit of the eggs was significantly increased in layers fed with 1.5% of probiotic supplemented diet (P<0.05). The different levels of inclusion of the food waste and probiotic supplementation had not effect on egg shell color, blood spot and albumen index of the eggs (P<0.05). There were not significant differences in over all acceptability, juiciness, texture and color of the eggs from layer fed with different levels of wood waste and probiotic supplementation. The appearance and flavor significantly deteriorated in eggs from layers fed with probiotic diets compared to control (P<0.05).

• Korean Journal of Poultry Science
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• v.21 no.1
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• pp.41-48
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• 1994

In order to get information for the improvement of feeding systems of egg-type layers, a dietary self-selection study was conducted. Corn diet, soybean meal diet, wheat bran diet, soybean meal+fish meal diet, and limestone diet were individually prepared for self-selection regime. Forty brown layers were offered either a control laying diet (C) or one of the 3 self-selection regime consisted of corn-soybean meal-limestone diet ($T_1$), corn-soybean meal-wheat bran-limestone diet ($T_2$) and corn-soybean meal+fish meal-wheat bran-limestone diet ($T_3$) for one wk each at 31 wk and 41 wk of age. In all dietary treatments, most of the eggs were laid from 0700 h 1300 h and hens consumed more feed, energy and protein on egg forming days than on non-forming days. On egg forming days, hens fed self-selection regime consumed less energy and protein than those fed control diet. On egg forming days, the consumption of feed, energy and protein increased continuously from 1000 h 2200 h in the control diet group but they decreased during the last 3 hours from 1900 h to 2200 h in self-selection regime groups. Up to 1600 h, calcium intake of the self-selection regime groups were much less than that of the control group but after that time, they increased markedly and were much more than that of the control group. The results of the study suggest that the practical self-selection regime for layers should be developed to meet the daily cyclic requirement for nutrients without consuming an excess energy and protein at certain times of the day.

• Korean Journal of Poultry Science
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• v.41 no.2
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• pp.135-142
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• 2014

This study was conducted to evaluate dietary effects of Marigold extract on laying performance, egg quality, oxidative stability of egg yolk and lutein transfer into chicken eggs. A total of one-hundred eighty nine 55-wk-old Hy-Line Brown layers were divided into seven groups and fed control diet or each experimental diet containing 0.1, 0.3, 0.5, 1.0, 1.5 or 2.0% Marigold extract. Egg production, egg weight and daily egg mass were not affected by dietary treatments. The yolk colors in groups fed diets containing Marigold extract were significantly higher than that of control. The Haugh unit were tended to be improved by feeding of diets containing Marigold extract although there were no significant difference in egg shell strength and thickness. The MDA (malondialdehyde) contents in groups fed diets containing Marigold extract above 0.5% were significantly reduced than that of control. After 14d of storage, the Haugh unit values in groups feed diets containing 0.3 and 1.0% Marigold extract were significantly higher than that of control (p<0.05). The concentration of lutein in egg yolk increased by feeding of Marigold extract. When 2% Marigold extract was supplemented to the diet, lutein content of egg was increased as much as 1.71 mg/60 g. These results indicated that the use of Marigold extract in layer diets was effective in egg quality and for the production of lutein fortified eggs.

• Food Science of Animal Resources
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• v.28 no.5
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• pp.645-650
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• 2008

The effects of various levels of dietary dried whole crop of kale (0.1%, 0.3%), dried peel of kale (0.3%), dried whole crop of Angelica keiskei (Koidz) (0.1%, 0.3%), and peel of Angelica keiskei (Koidz) (0.3%) in egg-laying performance were studied with 560 ISA brown layers for a period of 12 weeks. No significant differences were recognized between the treatments and control in egg production, feed intake and egg mass. The quality of egg and eggshell were not different among treatments but egg yolk color was improved in the both of whole crop and peel of kale and Angelica keiskei (Koidz). Vitamin and polyunsaturated fatty acid concentration of eggs increased in treatments compared to control but were not significantly different from controls. In conclusion, dried peel of kale and Angelica keiskei (Koidz) supplementation in laying hens diet improved egg yolk color, and improve vitamin concentration of chicken eggs.

• Korean Journal of Poultry Science
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• v.39 no.2
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• pp.157-161
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• 2012

The objective of this study was to evaluate the effects of feeding dried Thymus vulgaris powder (TVP) on the layer performance, egg quality and egg yolk fatty acid composition. Two hundred eighty eight, 35 weeks old Brown nicks were allocated in the cage for five TVP (0, 0.25, 0.5, 1.0 and 2.0%) diets with four replicates in each treatment. Each basal diet contained 16.0% CP and 2,800 kcal/kg ME and supplied to layers for 8 weeks. Egg production and egg weight were not statistically different but numerically increased in birds fed 2.0% TVP than other treatments. Egg mass was not consistent depending on the TVP addition levels in diets. Feed intake was significantly lower in TVP addition treatments than the control (p<0.05), however, feed conversion was not significantly differed among control and treatments (p<0.05). Dietary addition of 2.0% TVP showed high values on albumin height, Haugh unit and egg yolk color and they are 7.93, 86.79 and 7.20, respectively (p<0.05). Palmitic and arachidonic acids were remarkably lowered when 2.0% TVP added to basal diets and then fed to birds for 8 weeks, in contrast, significantly higher linolenic acid composition was determined in birds fed 2.0% TVP (p<0.05). As the results, the highest unsaturated fatty composition and UFA/SFA ratio were observed in 2.0% TVP treatment as compared to those of control and other treatments. Therefore, the results indicate that the Thymus vulgaris powder addition to basal diet tended to have positive effects on egg quality and egg yolk fatty acids composition, and the optimum addition of Thymus vulgaris powder may be 2.0%.