• 한국패류학회지
• /
• 제19권2호
• /
• pp.107-110
• /
• 2003

The effect of broodstock age on the spawning performance of cultured abalone, Haliotis discus hannai, was assessed for five age groups (3, 4, 5, 6, 7 years old). Spawning performance of the five age groups was assessed in terms of the percentage of spawning abalone, eggs per spawning female, fertilization rates, hatch rates and attachment rates per spawning. The percentage of spawning abalone increased along with broodstock age, reached maximum in female groups of 5- and 6-year age (60%), and in male group of 6-year age (100%), subsequently decreased in 7-year age group. The number of eggs produced per spawning also increased with broodstock age, and the maximum was found in 6-year age. Although the number of spawned eggs for the oldest abalone declined, it still spawned much more eggs than 3, 4, and 5 years old females. The fertilization and hatch rates were obviously larger for 4-6 years old group than the younger and older abalones. The maxima were found in 5-year age group (96.0% and 84.5%), and the minimum were appeared in 7-year age group (79.3% and 58.2%). The attachment rate increased with broodstock age, reached maximum in 6-year age group (33.6%), although the increment gradually declined. The attachment rate for 7-year age group rapidly decreased (16.6%). These results suggested that broodstock age affected the spawning performance of H. discus hannai, which peaked between 5 and 6 years old, and broodstock should be bred during this period for hatchery production.

• 현장농수산연구지
• /
• 제21권1호
• /
• pp.149-157
• /
• 2019

패류 인공종자생산에서 모패관리의 중요성이 커져가고 있으며 최적의 모패 관리 조건과 원하는 시기에 종자생산을 할 수 있는 성숙유도 조건을 구명하는 하는 것이 매우 중요하기 때문에 본 연구에서는 왕우럭 조개의 모패관리 최적조건과 성숙 촉진을 위한 가온 효과를 조사하였다. 모패관리는 실내사육에 비하여 실외사육이 비만도와 생존율 측면에서 더 효과적인 것으로 나타났으며 관리 측면에서도 인위적인 먹이생물 공급이 없으며 자연환경 조건에서 사육하기 때문에 관리가 수월하다는 장점이 있는 것으로 나타났다. 그리고 동계가온을 통한 성숙촉진 유도는 비만도와 생식소 관찰 등을 통하여 수온 18℃ 이상에서 2개월 정도 사육하였을 경우 성성숙이 이루어지는 것으로 나타나 성숙관리가 다른 패류에 비해 용이하다는 것을 알 수 있었다.

• 한국어류학회지
• /
• 제27권1호
• /
• pp.10-15
• /
• 2015

현재 IUCN의 취약 등급인 대왕바리(giant grouper, Epinephelus lanceolatus) 친어의 효율적인 관리 시스템 구축을 위한 기반연구로서 기 개발되어 있는 동종의 microsatellite 마커를 이용한 가계도 분석 효율을 조사하였다. 대왕바리 친어 32마리를 8개의 microsatellite 마커로 분석한 결과 총 52개의 대립유전자가 검출되었으며, 기대치 이형접합율은 0.663, 근친교배계수는 0.011로 조사되어 현재 확보된 대왕바리 친어는 유전 다양성이 비교적 잘 유지되고 있었다. 하지만 유효집단 크기가 35로 추정됨으로써 지속적인 친어 확보의 필요성을 보였다. 해당 마커를 이용한 동일 유전자형 출현 확률은 무작위 집단에서 $6.85{\times}10^{-11}$ 그리고 한쪽 부모의 유전자형 확보 및 양친의 유전자형이 확보된 상태에서의 부권 부정률은 각각 0.00835, 0.00027로 나타났으며, 주좌표 분석 결과 친어의 유전자형은 중복되지 않았다. 따라서 본 연구에 이용한 8개의 microsatellite 마커로도 유전자형 데이터베이스를 기반으로 한 대왕바리 친어 관리 시스템 구축이 가능할 것이며, 이를 활용한 유전 다양성이 높은 자손 생산 및 유전적으로 유사한 개체의 중복 확보를 방지할 수 있어 친어 확보의 효율성을 높일 수 있을 것이다.

• 한국어업기술학회:학술대회논문집
• /
• 한국어업기술학회 2001년도 춘계 수산관련학회 공동학술대회발표요지집
• /
• pp.99-100
• /
• 2001

Although cultured sweet smelt production recently have been rapidly increased from 89 metric tons (M/T) in 1997 to 500M/T In 1999 in Korea, wild fish have been still used as broodstock for cultured fish seed. This is considered due to having higher sperm and e99 qualify in wild fish broodstock than cultured fish. However, capturing a number of wild sweet smelt broodstock might lead to decreasing of the production of wild fish, In truth, of which production has been greatly decreased so that a number of artificial juvenile have been stocked some rivers in Korea. (omitted)

• 수산해양교육연구
• /
• 제26권4호
• /
• pp.695-704
• /
• 2014

조피볼락 친어 3개 그룹(서해자연산, 서해가두리양식산, 동해자연산)과 치어 5개 그룹(서해자연산, 서해육산양식산, 서해육상 가두리양식산, 서해 축제식 가두리양식산, 서해축제식양식산)에 대하여 사육방법과 크기의 차이에 따라서 9개의 혈청 화학성분이 어떠한 변화를 나타내는지 알아보고자 하였다. 그 결과, 7개 항목인 총단백, 알부민, 총콜레스테롤, AST, ALT, LDH, ALP의 함량에서 친어 및 치어그룹 간에 유의적인 차이가 관찰되었다. 즉, 총단백, 알부민, 총콜레스테롤, ALP는 친어그룹이 치어그룹에 비해 높은 농도를 보였으며, 반대로 AST, ALT, LDH는 치어그룹이 친어그룹에 비하여 높은 농도를 나타내었다. 이러한 결과는 사육방법과 크기(친어, 치어)가 서로 다른 조피볼락의 생리적인 변화와 관련이 있는 것으로 판단된다.

• 한국패류학회지
• /
• 제27권2호
• /
• pp.143-148
• /
• 2011

연구는 양질의 피조개 수정란 확보와 안정적 종묘생산을 위하여 피조개 모패의 먹이생물로 미세조류 3종 (Isochrysis galbana, Tetraselmis tetrathele, Phaeodactylum tricornutum) 을 단독 또는 혼합 공급하여 먹이생물에 따른 피조개 모패의 비만도, 생식소발달, 성숙유도율 및 생존율을 조사하였다. 사육 45일 경과 후 피조개 모패의 완숙기 출현율, 성숙유도율 및 생존율은 3종의 미세조류를 혼합 공급하거나 T. tetrathele를 단독공급한 실험구에서 가장 높았다.

• BMB Reports
• /
• 제40권4호
• /
• pp.501-510
• /
• 2007

Sex-related genes expressed in vitellogenic ovaries of the giant tiger shrimp, Penaeus monodon, were identified by an EST approach. A total of 1051 clones were unidirectionally sequenced from the 5 terminus. Nucleotide sequences of 743 EST (70.7%) significantly matched known genes previously deposited in the GenBank (E-value <$10^{-4}$) whereas 308 ESTs (29.3%) were regarded as newly unidentified transcripts (E-value >$10^{-4}$). A total of 559 transcripts (87 contigs and 472 singletons) were obtained. Thrombospondin (TSP) and peritrophin (79 and 87 clones accounting for 7.5 and 8.3% of clones sequenced, respectively) predominated among characterized transcripts. everal full length transcripts (e.g. cyclophilin, profillin and thioredoxin peroxidase) were also isolated. A gene homologue encoding chromobox protein (PMCBX, ORF of 567 nucleotides encoding a protein of 188 amino acids) which is recognized as a new member of the HP1 family was identified. Expression patterns of 14 of 25 sex-related gene homologues in ovaries and testes of P. monodon broodstock were examined by RT-PCR. Female sterile and ovarian lipoprotein receptor homologues were only expressed in ovaries whereas the remaining transcripts except disulfide isomerase related P5 precursor and adenine nucleotide translocator 2 were higher expressed in ovaries than testes of P. monodon broodstock. A homologue of ubiquitin specific proteinase 9, X chromosome (Usp9X) revealed a preferential expression level in ovaries than testes of broodstock-sized P. monodon (N = 13 and 11, P<0.05) but was only expressed in ovaries of 4-month-old shrimp (N = 5 for each sex).

• Journal of Microbiology and Biotechnology
• /
• 제21권11호
• /
• pp.1109-1115
• /
• 2011

A highly sensitive and specific TaqMan real-time PCR was used to quantify hepatopancreatic parvovirus (HPV) type III infections in wild broodstocks and hatchery-reared postlarvae (PL) of Fenneropenaeus chinensis. Totals of 159 and 162 wild brooders from three locations were captured, and 140 and 180 PL were obtained from seven and six commercial hatcheries in 2007 and 2008, respectively. Among the three wild broodstock groups from 2007, only 1 group showed HPV infection and 3.2% of 159 brooders were positive for HPV infection. In 2008, HPV infections were observed from all three wild broodstock groups with $1.93{\times}10^4$ copies/mg tissue of pleopods. Of 162 brooders, 26.6% were positive for HPV infection. No PL from the two hatcheries collected in 2007 showed HPV infection, and PL from the rest of the five hatcheries had up to $1.74{\times}10^6$ copies/ng of DNA, and PL from three hatcheries showed HPV infections with over 1,000 copies/ng of DNA. The PL from all seven hatcheries collected in 2008 showed up to $2.10{\times}10^5$ HPV copies/ng of DNA. PL from two hatcheries showed less than 100 copies/ng of DNA, but PL from the rest of the hatcheries showed HPV infections with over 1,000 copies/ng of DNA. These results show that HPV type III is widely distributed in Korea in addition to previously reported HPV type I, and they can be effectively detected by type-specific realtime PCR.

• 한국수산과학회지
• /
• 제53권6호
• /
• pp.894-899
• /
• 2020

This study investigated the effects of inclusion of pollock oil (PO) in the diet of broodstock on the egg and larval quality of Walleye Pollock Gadus chalcogrammus. The experimental diet comprised of semi-moist pellets prepared either with or without PO (control; Con). The fish assigned to each diet were fed to visual satiation (two meals per day) for 12 weeks. Eggs spawned from both groups of fish were sampled during the spawning season (three months) and the egg and larval quality was determined. Total egg volume, fertilization, and hatchability of eggs from the PO group were higher than those of the Con group. Fatty acid composition of fertilized eggs was not affected by the experimental diet. Oleic acid, eicosapentaenoic acid, and docosahexaenoic acid content in larvae of the PO group was higher than that in larvae of the Con group. These results demonstrate the beneficial effects of including PO in the broodstock diet of Walleye Pollock, specifically on the quality of the eggs (total egg volume, fertilization, and hatchability) and larvae (fatty acid composition).

• Journal of Microbiology and Biotechnology
• /
• 제11권3호
• /
• pp.394-398
• /
• 2001

Because of the great concern over the possibility of contamination from the rod-shaped nuclear virus (PRDV) from Japan and white spot virus (WSSV) from Taiwan, most eggs used in Korean shrimp farms are currently obtained from local broodstock. In addition, the screening of imported broodstock for any viral presence at the National Fisheries Research and Development Institute is also mandatory. Nonetheless, massive mortality from white spot syndrome continues in Korea. In the present study, we present an improved PCR method to use tissue-extracted DNA instead of viral DNA extracted from a purified virus based on a sucrose density gradient, and produced results within 8 h. In 1998, this modified PCR method was able to detect that diseased Penaeus japonicus were infected within 8 h. In 1998, this modified PCR method was able to detect that diseased Penaeus japonicus were infected only with PRDV, while Fenneropenaeus chinensis were infected with both PRDV and WSSV. In 1999, PRDV and WSSV were detected in F. chinensis with signs of infection, but not with WSSV alone.