• Korean Journal of Pharmacognosy
• /
• v.38 no.3 s.150
• /
• pp.227-233
• /
• 2007

Seven compounds were isolated from the n-BuOH soluble fraction of stem of Rhododendron mucronulatum as cytotoxic principles against brine shrimp lethality test. On the basis of spectral data, seven compounds were identified as (-)-catechin (1), (+)-epicatechin (2), taxifolin (3), scopoletin (4), quercetin (5), $taxifolin-3-O-{\alpha}-L- arabinopyranoside$ (6), and astragalin (7). Among tested compounds, $taxifolin-3-O-{\alpha}-L-arabinopyranoside$ (6) exhibited potent activity with $LC_{50}$ value at 4.6 ${\mu}g/ml$.

• Journal of Advanced Marine Engineering and Technology
• /
• v.30 no.3
• /
• pp.376-381
• /
• 2006

This study is experimented to observe an influence of experimental conditions on production characteristics of slurry ice by putting ball into test section to disturb ice adhesion. And at this experiment it used ethylene glycol-water solution and the concentration is 20wt%. The experimental apparatus was constructed of ethylene glycol-water solution and slurry ice storage tank. brine tank, pumps for ethylene glycol-water solution and brine circulating, a mass flow-meter data logger for fluid temperature measuring and a vertical circular tube with two copper tubes as test section. The experiments were carried out under various conditions, with mean velocity of fluid at the entry ranging from 0.07 to 0.13m/s and ball diameter is 10mm, 15mm. Also ball filling rate is 33%, 50%.

• Proceedings of the Korean Society of Marine Engineers Conference
• /
• 2006.06a
• /
• pp.49-50
• /
• 2006

This study experimented to observe an influence of experimental conditions on production characteristics of slurry ice by putting ball into test section to disturb ice adhesion. And at this experiment it used ethylene glycol-water solution and the concentration is 20wt%. The experimental apparatus was constructed of ethylene glycol-water solution and slurry ice storage tank, brine tank. pumps for ethylene glycol-water solution and brine circulating, a mass flow-meter, data logger for fluid temperature measuring and a vertical circular tube with two copper tubes as test section. The experiments were carried out under various conditions, with mean velocity of fluid at the entry ranging from 0.07 to 0.13m/s and ball diameter is 10mm, 15mm. Also ball filling rate is 33%, 50%.

• Archives of Pharmacal Research
• /
• v.21 no.2
• /
• pp.153-156
• /
• 1998

The crude extract of Saussurea lappa displayed significant lethality to brine shrimp larvae. Investigation of the causative components by bioactivity-directed fractionation resulted in the isolation of three $C_17$-polyene alcohols. Based on various nmr spectral data, these compounds were identified as shikokiols which had been previously isolated from Cirsium nipponicum and/or Centaurea aegyptica. These $C_17$-polyene alcohols exhibited moderate cytotoxicities against the human tumor cell lines, A549, SK-OV-3, SK-MEL-2, XF498, and HCT15.

• Korean Journal of Pharmacognosy
• /
• v.29 no.3
• /
• pp.193-197
• /
• 1998

Two isoflavonoids were isolated from the flowers of Pueraria lobata (Flos puerariae) guided initially fractionation based on brine shrimp lethality assay. The structures were identified as 4',5,7-trihydroxy-6-methoxyisoflavone (tectorigenin) and 4',7-dihydroxy-6-methoxyisoflavone (glycitein), respectively, on the basis of their spectroscopic and physicochemical evidences.

• Proceedings of the Korean Society of Applied Pharmacology
• /
• 1998.11a
• /
• pp.82-87
• /
• 1998

Brine shrimp lethality test has become one of our routine tools in selecting plant materials for further chemical or bioactivity studies in our laboratory. Usually, once a potentially bioactive sample has been identified, it will then be subjected to more elaborate bioassay procedures. Out of more than 200 plant samples tested we found eight samples to be toxic towards brine shrimp larvae.

• Proceedings of the Korea Society for Energy Engineering kosee Conference
• /
• 2004.05a
• /
• pp.219-224
• /
• 2004

The present study was to investigate the heat transfer enhancement effect of coil wire fins in an ice storage system. For the two cases of tubes with internal brine flows, a finned tube with coil wires on its outer surface and a smooth tube, the rates of ice layer were tested and compared for both the icing processes. The coil fins were made of a stainless steel wire with a dia. of 2.0(mm), and the coil had an outer dia. of 10(mm) and a helix angle of 60($^{\circ}$). The experimental results showed that the coil fins could substantially reduce the thermal resistance of the ice layer, and enhance the heat flow rates between the water in the storage tank and the brine. The ice storing time was found to be shortened by 13(%) for the coil fins to get the same amount of ice layer that was built on the smooth tube wall for 10 hours of operation when the same thermal conditions were provided.

• Archives of Pharmacal Research
• /
• v.22 no.5
• /
• pp.524-528
• /
• 1999

cis-Annonacin (1) and the mixture of (2,4)-cis-and trans-isoannonacins (2 and 3), three known mono-tetrahydrofuran annonaceous acetogenins, have been isolated form the seeds of Annona cherimolia by the use of the brine shrimp lethality test (BST) for bioactivity directed fractionation. Their structures were elucidated based on spectroscopic and chemical methods. 1 showed potent cytotoxicities in the brine shrimp lethality test (BST) and among six human solid tumor cell lines with notable selectivity for the pancreatic cell line (PaCa-2) at about 1,000 times the potency of adriamycin. The mixture of 2 and 3 is over 10,000 times cytotoxic as adriamycin in the pancreatic cell line (PaCa-2). All of the compounds are about 10 to 100 times as cytotoxic as adriamycin in the prostate cell line (PC-3).

• Journal of the East Asian Society of Dietary Life
• /
• v.14 no.5
• /
• pp.438-442
• /
• 2004

This study was conducted to investigate the chemical and microbiological properties of taurine added Kimchi during fermentation at 20℃. A control group was salted with 10% brine solution, and a taurine group was salted with 10% brine solution including 5 ％ taurine and was divided into two groups depending on added taurine concentration after brining; 0% taurine (Taurine I) and 3 ％ taurine added (Taurine II). The pH of Kimchi was markedly decreased over time in all groups and there was no significant difference between groups. Total acidity was the highest in Taurine II followed by Taurine I and. control group during five days of fermentation. The number of total microbe and lactic acid bacteria showed increase similarly in all groups.

• Archives of Pharmacal Research
• /
• v.24 no.4
• /
• pp.300-306
• /
• 2001

A novel and two known bioactive mono-tetrahydrofuran (THF) annonaceous acetogenins, annomocherin (1), annonacin (2) and annomontacin (3), have been isolated from the fractionated ethanolic extracts of the seeds of Annona cherimolia, guided by the brine shrimp lethality test (BST). Their structures were elucidated on the basis of spectroscopic and chemical methods. All compounds have a relative stereochemistry of threo/trans/threo for the mono-THF ring with two flanking hydroxyls. Compound 1 has a double bond at C-23/ 24 of aliphatic chain. Compound 1 was isolated from natural sources for the first time, and was named annomocherin. Two known Compounds 2 and 3 which have never been isolated from this species before, were obtained. Compound 1 exhibited potent and selective cytotoxicities against the breast carcinoma (MCF-7) and kidney carcinoma (A-498) cell lines with 100 to 1,000 times the potency of adriamycin. In brine shrimp lethality test (BST), 1-3 exhibited cytotoxicity.