• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2006년도 한국육종학회 공동심포지엄 및 학술대회
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• pp.5.1-5.1
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• 2006

• 대한수의학회지
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• 제14권2호
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• pp.253-268
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• 1974

A total of 331 dairy cattle in Jeonnam area were examined for the breeding status and hematological values during the period from June to August. 1971 and 1974. The data obtained were analysed according to the status of breeding and the type of farm management. The results obtained in this work were summarized as follows: 1. 331 dairy cows were grouped as pregnant (63.81%) anestrus after parturition (12.45%), pregnancy unknown (11.48%), repeat breeder (10.32%), and others (1.94%). 2. The summery of reproductive histories and clinical examination were as follows. Average of calving interval was 16.5 months, interval from parturition to first breeding 97 days and postpartum interval to first estrus 72 days. Services per conception was 1.6 rate of postpartum estrus (60 days) 12.0%, and the rate of repent breeder 10.3%. 3. Generally, the blood values of RBC, Hb, serum total protein and A/G ratio were lower than those normal values, especially, the cows which showed abnormal values belonged to the repeat breeder and the unknown to conception group. The mean value for serum inorganic phosphorus was the normal value or hyperphosphatemia, on the other hand, the mean value for serum calcium of the repeat breeder group was the lowest than the other group. 4. Follow-up evaluations on the results of the laboratory tests strongly suggest that the problems of repeat breeder had a tendency to occur more frequently in the large herd (A and B type farm), and the Ca/P ratio of almost all the cows showed abnormal values.

• 한국수정란이식학회지
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• 제32권3호
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• pp.171-176
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• 2017

본 연구는 한우 송아지 생산효율 향상을 위한 농가 번식 실태를 조사하였다. 한우 사육 규모별, 지역별로 나누어 45개 농가의 가임암소를 대상으로 조사를 실시하여 다음과 같은 결과를 얻었다. 한우의 최초 분만월령은 평균 28.7개월령이었다. 수태당 인공수정 횟수는 $1.45{\pm}0.32$회였으며, 분만 후 인공수정 일수는 119.8일이었다. 수태율은 소규모 농가가 $75.2{\pm}16.93%$로 중규모($70.6{\pm}17.46%$) 및 대규모 농가($71.4{\pm}11.03%$)보다 높은 것으로 나타났다. 번식률 상위 농가의 사육 방식을 보면, '발정관찰 보조기구를 사용하는 농가'가 '그렇지 않은 농가'보다 송아지 생산율이 10.42% 높았다. 번식소에 대해 IBR과 BVDV 예방접종을 실시한 농가는 유사산 폐사율이 4.41% 감소한 것으로 나타났다. 또한 농가여건에 따라 방목과 운동을 한 경우, 수태율과 분만율이 각각 3.47%, 18.29% 향상되는 것으로 나타났다. 본 조사 결과를 통해 발정관찰, 예방접종, 방목이 한우농가에서 송아지 생산 효율을 높이는데 중요한 지표로 나타났다. 한우 사육 농가의 번식실태 조사를 통해 농가 현장의 번식률 향상을 위한 연구 자료로 활용이 가능할 것이다.

• International Journal of Industrial Entomology and Biomaterials
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• 제15권2호
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• pp.145-155
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• 2007

Mulberry (Morus spp.) is an economically important tree crop being cultivated in India, China and other sericulturally important countries for its foliage to feed the silk producing insect Bombyx mori L. Genetic improvements of mulberry lag behind to the same in many other economically less important crops due to the complexity of its genetics, the breeding behavior, and the lack of basic information on factors governing important agronomic traits. In this review, the general usage and advantages of different molecular markers including isoenzymes, RFLPs, RAPDs, ISSRs, SSRs, AFLPs and SNPs are described to enlighten their applicability in mulberry genetic improvement programs. Application of DNA markers in germplasm characterization, construction of genetic linkage maps, QTL identification and in marker-assisted selection was also described along with its present status and future prospects.

• 현장농수산연구지
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• 제18권1호
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• pp.47-61
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• 2016

Ranging from a variety that a consumer to purchase and consume, and a variety with high quality and pest resistance, sustaining biological diversity as well as hiring global experts who understand breeding policy, it tightly maintains relationship with producer, consumer, and exporter to promote superior species for customized export to exporting country. By carrying out researches on cost saving and how to shorten the period of apple to cultivate, it consolidates its stance as a competent apple exporting country of apple and pear with high competetiveness. In order to compete against apple and pear of New Zealand, farmers argue that they need budget and researchers also argue that "No money, no research." Due to certain limit of governmental budget support, it is believed that it is time to fund our own way to promote apple research center. To come up with the best apple production, all of those who associates with apple such as farmers, researchers, government, exporters, and consumer should do their best to enhance the apple and pear industry.

• Asian-Australasian Journal of Animal Sciences
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• 제26권8호
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• pp.1072-1079
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• 2013

The NLRP12 (NLR family, pyrin domain containing 12) serves as a suppressor factor in the inflammatory response and protects the host against inflammation-induced damage. In the present study, we aimed to study the polymorphisms of NLRP12 gene and its association with susceptibility to non-specific digestive disorder (NSDD) in rabbits. We re-sequenced the entire coding region of the rabbit NLRP12 gene and detected a total of 19 SNPs containing 14 synonymous and five non-synonymous variations. Among them, the coding SNP (c.1682A>G), which would carry a potential functional implication, was subsequently subjected to genotyping for case-control association study (272 cases and 267 controls). The results revealed that allele A was significantly protective against NSDD with an odds ratio value of 0.884 (95% confidence interval, 0.788 to 0.993; p = 0.038). We also experimentally induced NSDD in growing rabbits by feeding a fibre-deficient diet and subsequently investigated NLRP12 mRNA expression. The mRNA expression of NLRP12 in healthy status was significantly higher than that in severe NSDD (p = 0.0016). The highest expression was observed in individuals carrying the protective genotype AA (p = 0.0108). These results suggested that NLRP12 was significantly associated with the NSDD in rabbits. However, the precise molecular mechanism of NLRP12 involving in the development of rabbit NSDD requires further research.

• Asian-Australasian Journal of Animal Sciences
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• 제21권8호
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• pp.1220-1225
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• 2008

This study was conducted to investigate the protective effect of genistein on the antioxidative defence system and membrane fluidity in chick skeletal muscle cells after supplementation with 0, 20, 40, and $80{\mu}mol/L$ genistein in $50{\mu}mol/L$ $FeSO_4/H_2O_2$ treated cells for 24 h. Genistein supplementation recovered the decreased activity of total superoxide dismutase induced by $FeSO_4/H_2O_2$, significantly increased glutathione peroxidase activity (p<0.05) and decreased malondialdehyde production (p<0.05). The treatment of 80 mol/L genistein in $FeSO_4/H_2O_2$ treated cells decreased the secretion of creatine kinase (p<0.05). Fluorescence polarization values and microviscosities observed with $FeSO_4/H_2O_2$ treated cells were significantly higher than those observed with no $FeSO_4/H_2O_2$ treated cells. The addition of $80{\mu}mol/L$ genistein improved the increased fluorescence polarization value (p<0.05) caused by $FeSO_4/H_2O_2$ treatment. The microviscosity value was significantly decreased by adding genistein (p<0.05). In conclusion, genistein protected skeletal muscle cells from oxidative damage by improving antioxidative status and membrane fluidity.

• Asian-Australasian Journal of Animal Sciences
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• 제19권9호
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• pp.1240-1244
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• 2006

The complete coding region sequence of the SRY gene in Chinese swamp buffalo was determined by PCR product sequencing. Comparison of swamp and river buffalo SRY gene sequences revealed a single nucleotide polymorphism (SNP, C/G) at the 202 bp site of the coding region. Further, a total of 124 male domestic buffaloes were genotyped at this SNP site using the PCR-SSCP method, and it was found that all Chinese indigenous swamp buffaloes had a guanine (G) at this site, while introduced river buffaloes and crossbred buffaloes showed a cytosine (C). Our findings suggested that this Y-linked SNP displayed type-specific alleles differentiating swamp and river buffaloes, and could be used as an effective marker to detect crossbreeding of swamp buffaloes with introduced river buffaloes in native buffalo populations, and thereby assess genetic diversity status and make proper conservation decisions for indigenous swamp buffaloes. In addition, this SNP can be potentially applied in the study of Asian water buffalo phylogeny from a male perspective.

• Asian-Australasian Journal of Animal Sciences
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• 제24권9호
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• pp.1192-1198
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• 2011

Peri-partum metabolic profile was evaluated in winter and summer calving, with 15 Murrah buffaloes in each seasonal group. In summer calvers, significantly lower values were observed for blood plasma urea nitrogen (BUN) at day 30 pre-partum (p<0.05), on calving day (p<0.05) and at all other stages (p<0.01); plasma non-esterified fatty acids (NEFA) values were significantly lower on day 30 pre-partum (p<0.01) and on day 60 post-partum (p<0.05). This was associated with significant reduction in days to first service (DFS) and service per conception (SPC) and an overall better reproductive performance in terms of service period, risk to first service on days 60, 90 and >90, and pregnancy risk to first service up to days 60 and 90. This may be attributed to better pre-partum nutritional status. Cervical and uterine involution were completed in fewer days, involutional changes took place at a faster pace and there were a lower number of abnormal involutional changes in winter compared to summer season. This may be attributed to better post-partum nutrition and less environmental stress. However, validation requires further targeted cohort investigation with a large sample size.

• Journal of Plant Biotechnology
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• 제42권4호
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• pp.298-311
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• 2015

포도는 전 세계적으로 가장 많이 재배되는 과수 작물 중의 하나로서 재배과정에서 많은 병해충이나 기상재해와 같은 스트레스에 직면한다. 과실의 고품질과 더불어 병충해 저항성인 품종 또는 내재해성 품종을 육성하는 것은 포도 생산에서 매우 중요한 과정이다. 고전적인 교배 육종을 이용한 신품종의 개발은 포장을 관리하는 데에 많은 노동력과 비용이 요구되며 오랜 시간이 소요되는 단점이 있다. 유용형질을 지닌 새로운 품종의 개발에 이용할 분자육종기술은 포도 육종프로램에서 전통적인 교배육종효율을 증진시킬 수 있는 매우 유용한 기술로 여겨진다. 포도의 유전체 해독을 완성함으로써 신품종육성에 활용될 유용유전자를 대량으로 발굴할 수 있고, 기능을 분석하는데 큰 도움을 주고 있다. 포도의 비교유전체, 전사체, 후성유전체, 유전체에 근거한 유전자 발굴, 분자마커 개발 등의 연구는 과실의 품질, 병해와 스트레스에 대한 저항성 기작을 구명하는데 중요한 단서를 제공하고 포도 육종에 유용하게 활용될 것이다.