• Korean Journal of Breeding Science
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• v.42 no.6
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• pp.699-702
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• 2010

Anthracnose is a major limiting factor of the watermelon production in Korea. A mid-late maturing watermelon line, 'Hanbit' with resistance to anthracnose (Colletotrichum orbiculare) race 1 and 3, was developed by the cross between anthracnose resistant cultivar 'AU-Producer' and high quality inbred line '920533' at the National Institute of Horticultural and Herbal Science, Rural Development Administration (RDA). 'Hanbit' produces 5.4 to 9.5 kg of red flesh fruits with clear stripes on skin. Average soluble solid contents are ranged from 10.4 to 11.2$^{\circ}$Bx. The yield and quality of 'Hanbit' was comparable to or better than those harvested from the popular commercial cultivars. 'Hanbit' can be cultivated in open fields and under the protected green house condition. In addition to the commercial production, it can be used as a resistant material in $F_1$ hybrid breeding program.

• 한국균학회소식:학술대회논문집
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• 2015.05a
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• pp.37-37
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• 2015

Kimchi cabbage (Chinese cabbage), radish and Cabbage are major Brassicaceae vegetables in Korea. Especially, we can easily develop whole plant from one microspore in Kimchi cabbage. To develop clubroot resistant doubled-haploid (DH) inbred lines, we pollinated a clubroot resistant turnip of 'IT 033820' with a Kimchi cabbage (Chinese cabbage) inbred of 'BP 079'. More than 85 DH inbred lines were developed from this combination. We screened about 400 materials including these DH inbred lines, commercial cultivars and breeding materials during 3 years using hydroponic system after inoculating single spore isolation race 4(SSI-04) inoculate. One inbred line derived from this combination selected as clubroot resistant and registered as 'Wonkyo20036ho'. We inoculated 26 DH inbred lines derived from 'Zoong-baek 2ho' using SSI-4, the percent of resistant plants varied from 0 to 83%. However the horticultural traits of highly resistant DH inbred line was poor. Thus we selected one DH line showing 77% resistant with yellow inner leaf and maid good head, was registered as 'Wonkyo20034ho'. Another DH inbred line derived from Korean variety of 'Wol-dong' showing 86% resistant was registered as 'Wonkyo20037ho'. Other DH inbred lines were derived from Chinese cultivar of 'Choon-hi-go-hang-wang' and 'Hwang-shim-zo48' showed 80 and 71% resistant, respectively, was also selected for registration. Even though DH inbred lines derived from turnip showed highly resistant to SSI-04 and provincial inoculate, they showed poor characteristics in horticultural traits. However, commercial seed companies showed interesting for adapting these DH inbred lines in commercial breeding.

• Molecules and Cells
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• v.23 no.2
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• pp.192-197
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• 2007

Bulb color in onions (Allium cepa) is an important trait whose complex inheritance mechanism involves epistatic interactions among major color-related loci. Recent studies revealed that inactivation of dihydroflavonol 4-reductase (DFR) in the anthocyanin synthesis pathway was responsible for the color differences between yellow and red onions, and two recessive alleles of the anthocyanidin synthase (ANS) gene were responsible for a pink bulb color. Based on mutations in the recessive alleles of these two genes, PCR-based markers for allelic selection were developed. In this study, genotype analysis of onions from segregating populations was carried out using these PCR-based markers. Segregating populations were derived from the cross between yellow and red onions. Five yellow and thirteen pink bulbs from one segregating breeding line were genotyped for the two genes. Four pink bulbs were heterozygous for the DFR gene, which explains the continuous segregation of yellow and pink colors in this line. Most pink onions were homozygous recessive for the ANS gene, except for two heterozygotes. This finding indicated that the homozygous recessive ANS gene was primarily responsible for the pink color in this line. The two pink onions, heterozygous for the ANS gene, were also heterozygous for the DFR gene, which indicated that the pink color was produced by incomplete dominance of a red color gene over that of yellow. One pink line and six other segregating breeding lines were also analyzed. The genotyping results matched perfectly with phenotypic color segregation.

• Korean Journal of Breeding Science
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• v.41 no.4
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• pp.587-590
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• 2009

New onion hybrid cultivar, "Yeongpunghwang" was developed from cross between "MOS8", male sterile line and "Mokpo 11", pollen line by Mokpo Sub-Station, National Institute of Horticultural & Herbal Science, RDA in 2008. The crossing line was performed yield test in 2003. IT was evaluated as "Mokpo 11" in the regional yield trial test(RYT) from 2006 to 2008 during three years. This line is semi-erect plant growth type and has green leaf colour. It has broad ovate bulb shape and yellow skin colour in bulb characteristics. Plant height, leaf number, and bolting rate was 78cm, 7.9, and 3.9%, respectively. Lodging date as harvesting indicator was on 26 May. The yield potential of "Yeongpunghwang" was out-yielding 6% compare to check variety ("Sunpower") by with 8,344 kg/10a in RYT. It showed moderated cold hardness in all regional location which was conducting RYT, including Muan, Goheung, Changyeong and Andong. So this variety was recommend as a leading variety at southern area of korea.

• Korean Journal Plant Pathology
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• v.14 no.3
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• pp.212-216
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• 1998

Capsicum pepper selections fro PI163192, PI241670, PI244670, PI271322, PI308787, PI322719, and PI369994 were confirmed to be non-hypersensitively resistant to race 3 of Xanthomonas campestris pv. vesicatoria. A resistant cultivar,‘SR’, was shown to be hypersensitive. Four Koran local cultivars, a cytoplasmic male sterile line (A-line) and its maintainer (B-line) were highly susceptible. The resistant selections and cultivars were crossed with a male sterile A-line (Smsms) and fertility of their F1 hybrids was examined by observing he pollen production, testing pollen germination, and quantifying the amount of pollen produced per anther to identify the genotype interacting with the male sterile cytoplasm. The seven resistant PI selections turned out to be restorers (N(S)MsMs) and‘SR’to be a maintainer (Nmsms).

• Journal of Ginseng Research
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• v.22 no.1
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• pp.11-17
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• 1998

To develop a new ginseng varieties with good quality and high yielding, a lot of individual ginseng plants were selected in the farmer's fields in 1972. Among them, a promising line, 7259-3-1, has been developed through comparative cultivation of several lines selected with pure line separation of local races in Korea Ginseng '||'&'||' Tobacco Research Institute. Preliminary and advanced yield trials were performed for 8 years. It was then designated as "KG 101" and tested in the regional yield and adaptation trials for 10 years (1981-1990). KG101 has a green stem with light violet and orange-yellow fruit and flowers 3-7 days later than local race, Takyungjong. Taproot of KG101 was longer than local race Jakyungjong, and root yield of KG101 was 9% higher than local race Jakyungjong. In red ginseng quality, the rates of Chun-Jeesam (Chun and Jee means 1st and 2nd grade, respectively) were 22.3% and 9.4% for KG101 and Jakyungjong, respectively. In these results, it was clarified that KG101 was superior ginseng line with good quality.y.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.1
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• pp.1-6
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• 2001

Additive and dominance genetic variances were estimated for purebred Landrace selected with line breeding from 1989 to 1995 at Miyazaki Livestock Experiment Station, Kawaminami Branch. Ten body measurements, two reproductive traits and fifteen carcass traits were analyzed with single-trait mixed model analysis. The estimates of narrow-sense heritabilities by additive model were in the range of 0.07 to 0.46 for body measurements, 0.05 to 0.14 for reproductive traits, and 0.05 to 0.68 for carcass traits. The additive model tended to slightly overestimate the narrow-sense heritabilities as compared to the additive and dominance model. The proportion of the dominance variance to total genetic variance ranged from 0.11 to 0.91 for body measurements, 0.00 to 0.65 for reproductive traits, and 0.00 to 0.86 for carcass traits. Large differences among traits were found in the ratio of dominance to total genetic variance. These results suggested that dominance effect would affect the expression of all ten body measurements, one reproductive trait, and nine carcass traits. It is justified to consider the dominance effects in genetic evaluation of the selected lines for those traits.

• Journal of Plant Biotechnology
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• v.45 no.3
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• pp.228-235
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• 2018

In this study, two pepper varieties, PRH1 (powdery mildew resistance line) and Saengryeg (powdery mildew resistance line), were resequenced using next generation sequencing technology in order to develop InDel markers. The genome-wide discovery of InDel variation was performed by comparing the whole-genome resequencing data of two pepper varieties to the Capsicum annuum cv. CM334 reference genome. A total of 334,236 and 318,256 InDels were identified in PRH1 and Saengryeg, respectively. The greatest number of homozygous InDels were discovered on chromosome 1 in PRH1 (24,954) and on chromosome 10 (29,552) in Saengryeg. Among these homozygous InDels, 19,094 and 4,885 InDels were distributed in the genic regions of PRH1 and Saengryeg, respectively, and 198,570 and 183,468 InDels were distributed in the intergenic regions. We have identified 197,821 polymorphic InDels between PRH1 and Saengryeg. A total of 11,697 primers sets were generated, resulting in the discovery of four polymorphic InDel markers. These new markers will be utilized in order to identify disease resistance genotypes in breeding populations. Therefore, our results will make a one-step advancement in whole genome resequencing and add genetic resource datasets in pepper breeding research.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.60 no.3
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• pp.293-299
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• 2015

High temperature is one of major environmental stress. Heat tolerance managing is difficult through the phenotypic selection, so marker assistant selection (MAS) using molecular markers like as RAPD, SSR etc. was tried to select useful traits for heat tolerance. Fourteen SSR markers reported by previous research were selected for this research. We tried to evaluate 14 SSR markers for MAS using 31 useful wheat resources including 24 crossing line from Turkey, six Korean wheat cultivars and Chinese spring. The average of the number of alleles and PIC values in this study were 6.14 and 0.64, respectively. Two major clades and four sub clades were grouped by phylogenetic tree using UPGMA. Four Korean wheat cultivars were distinct from other Turkey resources in the phylogenetic dendrogram. From the results, we expected that these markers were able to adapt to screening wheat genotyping for heat tolerance.

• Korean Journal of Medicinal Crop Science
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• v.20 no.4
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• pp.277-285
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• 2012

In this study, Expressed Sequence Tag-Simple Sequence Repeat (EST-SSR) analyses were used to clarify the genetic polymorphisms among Korean ginseng cultivars and breeding lines and to classify them into distinct genetic groups. Polymorphic and reproducible bands were produced by 14 primers out of total 30 primers used in this study. Fourteen EST-SSR loci generated a total of 123 bands. Amplified PCR products showed the highly reproducible banding patterns at 110~920 bp. The number of amplified bands for each EST-SSR primers ranged from 2 to 19 with a mean of 8.8 bands. P26 and P35 primers showed 13 and 12 banding patterns, respectively. The number of alleles for each EST-SSR locus ranged from 1.67 to 2.00 with a mean of 1.878 alleles. P34 and P60 primers showed the highest and the lowest genetic polymorphism, respectively. Cluster analysis based on genetic similarity estimated by EST-SSR markers classified Korean cultivars and breeding lines into 4 groups. Group included Gopoong and Chunpoong and 9 breeding lines (55%), group included 2 breeding lines (10%), group included 3 breeding lines (15%), group included Gumpoong and 3 breeding lines (20%). Consequently, the EST-SSR marker developed in this study may prove useful for the evaluation of genetic diversity and differentiation of Korean ginseng cultivars and breeding lines.