KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
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Evaluate of SSRs for Heat Tolerance using Korean Major Wheat Cultivars and Heat Resistant Turkey Resources

주요 국산밀 품종과 내고온성 터키 유전자원을 이용한 내고온성 관련 SSR 마커 평가

  • Son, Jae-Han (Crop Breeding Division, National Institute of Crop Science, RDA) ;
  • Kim, Kyeung-Hoon (Department of Southern Area, National Institute of Crop Science, RDA) ;
  • Cheong, Young-Keun (Crop Breeding Division, National Institute of Crop Science, RDA) ;
  • Park, Jong-Chul (Crop Breeding Division, National Institute of Crop Science, RDA) ;
  • Kim, Kyong-Ho (Crop Breeding Division, National Institute of Crop Science, RDA) ;
  • Kim, Yang-Kil (Crop Breeding Division, National Institute of Crop Science, RDA) ;
  • Oh, Young-Jin (Crop Breeding Division, National Institute of Crop Science, RDA) ;
  • Song, Tae-Hwa (Crop Breeding Division, National Institute of Crop Science, RDA) ;
  • Kim, Bo-kyeong (Crop Breeding Division, National Institute of Crop Science, RDA) ;
  • Kang, Chon-Sik (Crop Breeding Division, National Institute of Crop Science, RDA)
  • 손재한 (농촌진흥청, 국립식량과학원, 작물육종과) ;
  • 김경훈 (농촌진흥청, 국립식량과학원, 남부작물부) ;
  • 정영근 (농촌진흥청, 국립식량과학원, 작물육종과) ;
  • 박종철 (농촌진흥청, 국립식량과학원, 작물육종과) ;
  • 김경호 (농촌진흥청, 국립식량과학원, 작물육종과) ;
  • 김양길 (농촌진흥청, 국립식량과학원, 작물육종과) ;
  • 오영진 (농촌진흥청, 국립식량과학원, 작물육종과) ;
  • 송태화 (농촌진흥청, 국립식량과학원, 작물육종과) ;
  • 김보경 (농촌진흥청, 국립식량과학원, 작물육종과) ;
  • 강천식 (농촌진흥청, 국립식량과학원, 작물육종과)
  • Received : 2015.07.01
  • Accepted : 2015.08.25
  • Published : 2015.09.30
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Abstract

High temperature is one of major environmental stress. Heat tolerance managing is difficult through the phenotypic selection, so marker assistant selection (MAS) using molecular markers like as RAPD, SSR etc. was tried to select useful traits for heat tolerance. Fourteen SSR markers reported by previous research were selected for this research. We tried to evaluate 14 SSR markers for MAS using 31 useful wheat resources including 24 crossing line from Turkey, six Korean wheat cultivars and Chinese spring. The average of the number of alleles and PIC values in this study were 6.14 and 0.64, respectively. Two major clades and four sub clades were grouped by phylogenetic tree using UPGMA. Four Korean wheat cultivars were distinct from other Turkey resources in the phylogenetic dendrogram. From the results, we expected that these markers were able to adapt to screening wheat genotyping for heat tolerance.

밀의 등숙기간, 종자 수, 종자무게와 관련된 SSR 마커 14개를 이용하여 터키에서 분양받은 내고온성 유전자원 23개와 국산밀 품종 7개, Chinese spring 1개 등 31개를 분석한 결과, 전체 86개의 대립유전자(평균 6.14개)가 확인되었다. 평균 PIC 값은 0.64로 나타났다. 다형성 분석을 통한 마커 데이터를 이용하여 계통 분석을 한 결과 크게 세 그룹으로 형성되었다. 올밀이 가장 바깥 그룹으로 형성되었고 올그루, 고소, 조품 등이 터키자원과 다르게 단일 그룹으로 형성되었다. 금강, 조경, 백중 등 세 품종은 터키 자원들과 같은 그룹으로 나타났지만 밀접하게 연관되어 있지는 않은 것으로 나타났다. 국내 품종 중 올, 올그루, 조품, 고소 등 4개와 금강, 조경, 백중 등 3개가 서로 다른 그룹으로 형성되었다. 두 그룹의 차이는 파성 II와 III의 차이로 구별되었다.

Keywords

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