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Phylogeny of the family Ophioglossaceae with special emphasis on genus Mankyua (제주고사리삼을 중심으로한 고사리삼과 식물의 계통)

  • Sun, Byung-Yun;Baek, Tae Gyu;Kim, Young-Dong;Kim, Chan Soo
    • Korean Journal of Plant Taxonomy
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    • v.39 no.3
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    • pp.135-142
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    • 2009
  • Phylogeny of the family Ophioglossaceae and a phylogenetic position of Mankyua were estimated through analyses of chloroplast rbcL gene sequences and spore morphology. Sequence analysis of the rbcL gene clearly indicated that there are two major lineages in the family Ophioglossaceae: Botrychioid lineage and Ophioglossoid lineage. The Botrichioid lineage is composed of three distinct clades: Botrychium, Helminthostachys and Mankyua, where Helminthostachys and Mankyua were placed as sister groups to the Botrychium. Within the genus Botrychium, subgenera Septridium and Botrychium were monophyletic, while taxa of subgen. Botrypus branched as sister of the two, successively, thus making a non-monophyletic group. Ophioglossum formed the Ophioglossoied lineage, where the subgen. Ophioglossum is monophyletic, while subgen. Cheiroglossa and Ophoderma formed a sister relationship with subgen. Ophioglossum. In terms of external morphology and spores, Mankyua is most similar to Helminthostachys, however, patristic distance in the cladogram and trophophore characteristics of the two genera are distinct. Therefore, Mankyua is a well defined genus within the family in terms of morphology as well as molecular phylogeny which places it in basal position of the Botrychioid lineage on the gene tree.

Immunostimulatory activity and structural characteristics of neutral polysaccharides isolated from ginseng leaves fermented by Cordyceps sinensis (동충하초균으로 발효한 인삼잎에서 분리한 중성다당의 면역활성 및 구조적 특성)

  • Cha, Ha Young;Park, Hye-Ryung;Shin, Kwang-Soon
    • Korean Journal of Food Science and Technology
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    • v.53 no.5
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    • pp.570-577
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    • 2021
  • This study elucidated the biological activities and structural characteristics of polysaccharides isolated from ginseng leaves fermented using Cordyceps sinensis (GLF). GLF comprised at least 18 glycosyl linkages, including 4-linked glucose residues (24.0%). To characterize the neutral polysaccharides in GLF, it was further fractionated by anion exchange chromatography, and the unabsorbed fraction (GLF1) was isolated. Peritoneal macrophages stimulated with GLF1 produced various cytokines in a dose-dependent manner. The properties and activities of the four subfractions (PHI, PHIA1-PHIA3) obtained after sequential enzymatic digestion were examined. PHI and PHIA3 primarily comprised glucose, whereas PHI exhibited an iodine-color reaction. Furthermore, the PHIA1-3 fractions indicated that cytokine production was completely inhibited. These results suggest at the immune activities of GLF1 may be due to the α-(1→4)-glucan branched at the C(O)6 position, which was produced by C. sinensis.

An Omnidirectional High Gain Antenna for UHF Band Ground Station (UHF대역 지상국용 무지향 고이득 안테나)

  • Bae, Ki-Hyoung;Chang, Min-Soo;Joo, Jae-Woo;Hwang, Chan-Ho;Hong, Ki-Pyo
    • Journal of the Korea Knowledge Information Technology Society
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    • v.12 no.4
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    • pp.539-550
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    • 2017
  • In this paper, we designed, fabricated and tested an UHF band cylindrical dipole array antenna. In the proposed antenna, cylindrical dipoles were vertically arranged in four stages. A parallel structure feeding circuit was installed inside the cylindrical dipole and mounted so as to be broadband matching. The feeding circuit was installed at the center of the cylindrical dipole to optimize the gain flatness characteristic of the azimuth direction omnidirectional radiation pattern. Minimizing the difference between the signals branched from the feeding circuit and realizing the symmetry of the radiation pattern. The required specifications are more than 11.2% bandwidth in UHF band, above 6dBi antenna gain, standing wave ratio of 2:1 or less, less than ${\pm}1dB$ gain flatness in azimuth radiation pattern, more than 13 degrees in elevation radiation pattern of 3dB beamwidth. We confirmed the possibility of implementation through M&S and verified the result of M&S through production and testing. The test results are 11.2% bandwidth in the UHF band, 6.30 to 8.31 dBi gain, 1.53:1 standing wave ratio or less, within ${\pm}0.2dB$ gain flatness in the azimuth radiation pattern, elevation radiation pattern of 3dB beam width was 15.62 to 15.84 degrees. The test result meets all requirements specifications.

Vietnamese Language Classes to Develop Prospective Teachers' Global-Multicultural Competences (예비교사들의 베트남어 습득을 위한 강좌: 글로벌·다문화 교육역량 육성의 일환)

  • Yi, Yunyoung;Bae, Jungok
    • The Journal of the Convergence on Culture Technology
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    • v.7 no.1
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    • pp.469-486
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    • 2021
  • Vietnam is a country where the largest number of Korean companies have branched out, and calibers who can speak Vietnamese are on great demands. In the multicultural classroom, students of Vietnamese background occupy the first or second largest composition among students with multicultural backgrounds; therefore, if teachers can teach in Vietnamese, the positive effects can be promising. This study presents and evaluates a Vietnamese language class developed and operated for the first time in Korea by a university to raise prospective teachers' global-multicultural competences. Two series of the classes were conducted during two vacations of 2018, and 20 students participated. As a results, the groups showed a significant increase in the global-multicultural competences; About 95 percent of the participants demonstrated improvement in the competences. The participants stated opinions: the classes should expand to offer opportunities for many other students who may want to acquire Vietnamese; the participants had better understandings of the Vietnamese cultures, envisioned their role as a Vietnamese speaker and suggested extended opportunities for contacts with Vietnamese cultures. The study hopes that other universities apply the educational model and the case study and that, as a result, teachers can contribute to building harmonious Korean-Vietnamese relations using Vietnamese.

Comparison and analysis on sheep meat quality and flavor under pasture-based fattening contrast to intensive pasture-based feeding system

  • Zhang, Zhichao;Wang, Xiaoqi;Jin, Yan;Zhao, Kai;Duan, Ziyuan
    • Animal Bioscience
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    • v.35 no.7
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    • pp.1069-1079
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    • 2022
  • Objective: The objective of this study was to investigate the effect of 4-month intensive feeding on the meat quality, fatty acid profile, flavor, and growth performance of grazing Hulunbuir sheep (HBS). Methods: The HBS were selected 4-months after birth in a pasture rearing system as the experimental animals (n = 44, female, average body weight 23.8±2.2 kg) then divided equally into pasture-based grazing fattening (PAS) and concentrate-included intensive fattening (CON) groups for another 4-month finishing. When finished fattening, all animals were slaughtered to collect musculus longissimus dorsi subcutaneous adipose tissue and to investigate the influences on meat quality, fatty acid profile, flavor and growth performance. Results: The results showed lambs in CON group got significantly higher live weight, hot carcass weight, and dressing percentage. The CON group had significantly higher value of redness (a*), lightness (L*) and water holding capacity (p<0.05), significantly lower value of Warner-Bratzler shear force than the PAS group (p<0.05). The subcutaneous fat from CON group lambs demonstrated a significantly higher content of C18:1 and C18:2 (p<0.05), but lower C14:0 and C16:0, indicating an increased degree of unsaturated fatty acid. The content of 4-methyloctanoic acid, 4-ethyloctanoic acid and 4-methylnonanoic acid had increased 2 to 4 times, representing a more intense odor in the CON group. However, the values were still lower than most sheep breeds reported, indicating the indoor feeding system could not fundamentally deteriorate the excellent meat characteristic of HBS. Conclusion: It was evident that lambs in CON group exhibited a better meat production performance, improved in meat color, texture and healthier fatty acid profile through pasture-weaned concentrate included intensive fattening system, which offers a good alternative regimen for lamb finishing and has a wide prospection in the HBS meat industry.

Community Structure and Ecological Characteristics of Berchemia berchemiaefolia Stands at Mt. Naeyon (내연산 망개나무 임분의 군집구조와 생태적 특성)

  • Yong Sik, Hong;I-Seul, Yun;Dong Pil, Jin;Chan Beom, Kim;Hak Koo, Kim;Jin Woo, Lee;Shin Koo, Kang
    • Journal of Korean Society of Forest Science
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    • v.111 no.4
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    • pp.538-547
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    • 2022
  • In this study, the population and community structure of Berchemia berchemiaefolia stands located at Mt. Naeyon (Gyeongbuk, Korea) were quantified, and multivariate analysis was done to determine the correlations between vegetation group types and environmental factors and to have reference data for the conservation and restoration of this species. In total, there were 164 B. berchemiaefolia trees in Mt. Naeyon. The average DBH of the trees was 24.5 cm, forming a normal distribution. It rarely appeared in an understory vegetation height of 3 m. About37.1% of the trees were branched. B. berchemiaefolia stands were classified into two groups: B. berchemiaefolia-Quercus serrata community and B. berchemiaefolia-Carpinus laxiflora community. Canopy gap, organic matter, exchangeable Ca, and cation exchange capacity were the major site characteristics affecting the distribution pattern of the stands. Currently, B. berchemiaefolia trees dominate in Mt. Naeyon, but depending on different habitat positions, the species was in a natural successional stage to C. laxiflora or C. cordata, which is a shade-tolerant species.

New record and prediction of the potential distribution of the invasive alien species Brassica tournefortii (Brassicaceae) in Korea (국내 침입외래식물 사막갓(Brassica tournefortii; Brassicaceae)의 보고 및 잠재 분포 예측)

  • KANG, Eun Su;KIM, Han Gyeol;NAM, Myoung Ja;CHOI, Mi Jung;SON, Dong Chan
    • Korean Journal of Plant Taxonomy
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    • v.52 no.3
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    • pp.184-195
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    • 2022
  • The invasive alien species Brassica tournefortii Gouan (Brassicaceae) is herein reported for the first time in Korea, from Gunsan-si, Gochang-gun, and Jeju-si. Brassica tournefortii can easily be distinguished from B. juncea and B. napus by its dense stiff hairs at the base of the stem and leaves, basally and distally branched stems, partially dehiscent fruits, and seeds that become mucilaginous in the presence of moisture. Although some taxonomists have classified this species as belonging to Coincya Rouy based on its fruit and seed characteristics, the existence of one vein on the fruit valves and our maximum likelihood analysis using internal transcribed spacer sequences placed it in Brassica. Distribution data, photographs, and a description of B. tournefortii are presented herein. Moreover, potential changes in the distribution of B. tournefortii were predicted under different climate scenarios, but our analysis showed that the probability of the spreading of this species is low. Nevertheless, continuous monitoring is necessary for an accurate assessment. The results of the present study can be used to conduct an invasion risk assessment and can assist with the effective management of this invasive alien species.

Effects of L-glutamine supplementation on degradation rate and rumen fermentation characteristics in vitro

  • Suh, Jung-Keun;Nejad, Jalil Ghassemi;Lee, Yoon-Seok;Kong, Hong-Sik;Lee, Jae-Sung;Lee, Hong-Gu
    • Animal Bioscience
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    • v.35 no.3
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    • pp.422-433
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    • 2022
  • Objective: Two follow-up studies (exp. 1 and 2) were conducted to determine the effects of L-glutamine (L-Gln) supplementation on degradation and rumen fermentation characteristics in vitro. Methods: First, rumen liquor from three cannulated cows was used to test L-Gln (50 mM) degradation rate and ammonia-N production at 6, 12, 24, 36, and 48 h after incubation (exp. 1). Second, rumen liquor from two cannulated steers was used to assess the effects of five levels of L-Gln including 0% (control), 0.5%, 1%, 2%, and 3% at 0, 3, 6, 12, 24, 36, and 48 h after incubation on fermentation characteristics, gas production, and degradability of nutrients (exp. 2). Results: In exp. 1, L-Gln degradation rate and ammonia-N concentrations increased over time (p<0.001). In exp. 2, pH was reduced significantly as incubation time elapsed (p<0.001). Total gas production tended to increase in all groups as incubation time increased. Acetate and propionate tended to increase by increasing glutamine (Gln) levels, whereas levels of total volatile fatty acids (VFAs) were the highest in 0.5% and 3% Gln groups (p<0.001). The branched-chain VFA showed both linear and quadratic effects showing the lowest values in the 1% Gln group particularly after 6 h incubation (p<0.001). L-Gln increased crude protein degradability (p<0.001), showing the highest degradability in the 0.5% Gln group regardless of incubation time (p<0.05). Degradability of acid detergent fiber and neutral detergent fiber showed a similar pattern showing the highest values in 0.5% Gln group (p<0.10). Conclusion: Although L-Gln showed no toxicity when it was supplemented at high dosages (2% to 3% of DM), 0.5% L-Gln demonstrated the positive effects on main factors including VFAs production in-vitro. The results of this study need to be verified in further in-vivo study.

Taxonomic Review of Cottiusculus nihonkaiensis (Cottoidei: Cottidae) from Korea (한국산 점줄횟대 Cottiusculus nihonkaiensis(둑중개아목, 둑중개과)의 분류학적 재검토)

  • Ju-Won Jin;ChungBae Kang;Jin-Koo Kim
    • Korean Journal of Ichthyology
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    • v.35 no.2
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    • pp.141-147
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    • 2023
  • The genus Cottiusculus Jordan and Starks 1904 (Cottoidei: Cottidae) comprises four species worldwide, three species in Japan, and two species in Korea. Cottiusculus species are characterized by a compressed head and body without scales, except for lateral-line scales, a preopercle with four spines, a curved uppermost preopercular spine with small dorsal spines, and teeth on the upper jaws, vomer, and palatines. Two Cottiusculus species have been recorded in Korea: Cottiusculus gonez and Cottiusculus nihonkaiensis. Among these, "jeom-jul-hoet-dae" specimens were initially identified as Cottiusculus schmidti by many Korean Ichthyologists. However, since C. nihonkaiensis was reported by Kai and Nakabo (2009), most such specimens have been identified as C. nihonkaiensis. To clarify the taxonomic status of "jeom-jul-hoet-dae", we conducted morphological and molecular analyses collected from the coasts of South Korea, and compared our findings with previous reports. Japanese and Korean C. nihonkaiensis specimens mostly consistent in some proportional measurements, our molecular results indicate that they are the same species. C. nihonkaiensis, "jeom-jul-hoet-dae" distinguished from other congers by combination of following characters: simple nasal spines, two branched cirri at the upper jaw, cirri at the opercular, preopercular, and lateral lines, and a number of blotches below the lateral line.

Taxonomic Status of Acheilognathus sp. HR (Cypriniformes: Acheilognathidae) Found in the Dalcheon River, a Tributary of Hangang River, Korea (한강 지류 달천에서 발견된 Acheilognathus sp. HR (Cypriniformes: Acheilognathidae)의 분류학적 위치)

  • Yong Hwi Kim;Bong Han Yun;Mu Sung Sung;In-Chul Bang
    • Korean Journal of Ichthyology
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    • v.34 no.4
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    • pp.231-243
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    • 2022
  • To investigate the taxonomic status of undescribed species Acheilognathus sp. HR from Korean Acheilognathidae discovered from the Dalcheon River, a tributary of the Hangang River, molecular phylogenetic and morphological characteristics were compared and analyzed with previous studies. As a result of molecular phylogenetic analysis, A. sp. HR formed the same genetic clade as the five subspecies of Acheilognathus tabira, but formed a separate monophyletic group based on the unique genotype, showing clear differences. As a result of morphological analyses, the dorsal fin color in males is grayish and the nuptial coloration of the outer edge of the anal fin is white. The outer edges of the dorsal and anal fins are convexly rounded. A black blotch is present on the dorsal fin of the juvenile, but there is a black blotch absent on the dorsal fin of the small adult female. In the counts, the number of branched dorsal rays is 12~13. In the measurements, the length of the barbels is short and the body depth is deep. Therefore, the A. sp. HR of Hangang River is considered at the level of a distinct species distinguished from each other by the five subspecies of A. tabira by molecular phylogenetic, morphological, and limited distributional characteristics.