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http://dx.doi.org/10.9721/KJFST.2021.53.5.570

Immunostimulatory activity and structural characteristics of neutral polysaccharides isolated from ginseng leaves fermented by Cordyceps sinensis  

Cha, Ha Young (Department of Food Science and Biotechnology, Kyonggi University)
Park, Hye-Ryung (Department of Food Science and Biotechnology, Kyonggi University)
Shin, Kwang-Soon (Department of Food Science and Biotechnology, Kyonggi University)
Publication Information
Korean Journal of Food Science and Technology / v.53, no.5, 2021 , pp. 570-577 More about this Journal
Abstract
This study elucidated the biological activities and structural characteristics of polysaccharides isolated from ginseng leaves fermented using Cordyceps sinensis (GLF). GLF comprised at least 18 glycosyl linkages, including 4-linked glucose residues (24.0%). To characterize the neutral polysaccharides in GLF, it was further fractionated by anion exchange chromatography, and the unabsorbed fraction (GLF1) was isolated. Peritoneal macrophages stimulated with GLF1 produced various cytokines in a dose-dependent manner. The properties and activities of the four subfractions (PHI, PHIA1-PHIA3) obtained after sequential enzymatic digestion were examined. PHI and PHIA3 primarily comprised glucose, whereas PHI exhibited an iodine-color reaction. Furthermore, the PHIA1-3 fractions indicated that cytokine production was completely inhibited. These results suggest at the immune activities of GLF1 may be due to the α-(1→4)-glucan branched at the C(O)6 position, which was produced by C. sinensis.
Keywords
Ginseng leaves; Cordyceps sinensis; fermentation; structural characteristics; ${\alpha}$-glucan;
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