• Annals of Clinical Neurophysiology
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• 제1권2호
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• pp.147-153
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• 1999

Hippocampal slice models can be a powerful tool to study the mechanism of partial epilepsy. Despite the loss of connection with the rest of the brain, in vitro hippocampal slice preparations allow detailed physiological and pharmacological studies, which would be impossible, in vivo. There are several methods to induce electrographic seizures on hippocampal slice models. Those are electrical pulse train stimulation, 0 $Mg^{2+}$ artificial cerebrational fluid and high concentration of extracelluar $K^+$ on bath. Among them, the electrically triggered seizure may mimic the physiological communication between neuronal populations without any deterioration of normal physiologic and chemical status of the hippocampal slice models. Presumably, such communication from hyperexcitable areas to other neuronal populations is involved in the development of epilepsy. Electrographic seizures in hippocampal slice models occur in the network of neurons that are involved in epileptic seizures in the hippocampus in vivo. Because these models have many advantages and are very valuable to research of epileptogenesis on partial epilepsy, I would like to introduce the electrophysiological methods to induce electrographic seizure or epilepsy on hippocampal slice models briefly in this paper.

• Archives of Pharmacal Research
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• 제28권10호
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• pp.1164-1169
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• 2005

Brain microglia are phagocytic cells that are the major inflammatory response cells of the central nervous system and widely held to play important pathophysiologic roles in Alzheimer's disease (AD) in both potentially neurotoxic responses and potentially beneficial phagocytic responses. In the study, we examined whether ginsonoside Rg3, a by-product of red ginseng, enhances the microglial phagocytosis of $A{\beta}$. We found that Rg3 promoted $A{\beta}$ uptake, internalization, and digestion. Increased maximal $A{\beta}$ uptake was observed at 4 and 8 h after Rg3 pretreatment (25 ${\mu}g/mL$), and the internalized $A{\beta}$ was almost completely digested from cells within 36 h when pretreated with Rg3 comparing with single non-Rg3-treated groups. The expression of MSRA (type A MSR) was also up-regulated by Rg3 treatment in a dose- and time-dependent manner which was coincidently identified in western blots for MSRA proteins in cytosol. These results indicate that microglial phagocytosis of $A{\beta}$ may be enhanced by Rg3 and the effect of Rg3 on promoting clearance of $A{\beta}$ may be related to the MSRA-associated action of Rg3. Thus, stimulation of the MSRA might contribute to the therapeutic potentials of Rg3 in microglial phagocytosis and digestion in the treatment of AD.

• Journal of Acupuncture Research
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• 제17권3호
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• pp.168-175
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• 2000

Stimulation of the auricle is known to be effective in reducing obesity. The aim of the present study is to investigate whether stimulating a specific auricular acupuncture point is effective on suppression of appetite. Cholesystokinin (CCK) is known to induce a powerful feeding response after central administration and particularly abundant in the cerebral cortex. In food-deprived rats exhibiting a strong drive for feeding, decreased CCK release was markedly detected in the cerebral cortex of the brain. Needling the fasted rats on the specific auricular region increased the CCK level of the cerebral cortex. In conclusion, stimulating the specific auricular points increases CCK-expression in the cerebral cortex of the fasted rats and may be useful for controlling obesity.

• 약학회지
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• 제41권6호
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• pp.773-781
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• 1997

The activity of $Ca^{2+}$calmodulin (CaM)-dependent protein kinase Ia (CaM kinase Ia) is shown to be regulated through direct phosphorylation by CaM kinase I kinase (CaMK IK). In the present study, three distinct CaMKIK peaks were separated from Q-Sepharose colunm chromatography of pig brain homogenate using a Waters 650 Protein Purification System. The purified CaMKIK from the major peak potently and rapidly enhanced CaM kinase Ia activity, reaching a maximal stimulation within 2min at the concentrations of 12-15nM. The activated state of CaM kinase Ia is characterized by a markedly enhanced $V_{max}4 as well as significantly decreased $K_m\;and\;K_a$ values toward peptide substrate and CaM, respectively. These observations suggest the activation process of CaM kinase Ia. The phosphorylation of CaM kinase Ia by CaMKIK may induce its conformational change responsible for the alterations in the kinetic properties, which ultimately leads to the rapid enzyme activation.

• Journal of International Society for Simulation Surgery
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• 제3권1호
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• pp.22-27
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• 2016

Background Craniosynostosis management using distraction osteogensis represent a challenge for surgeons due to the great variability of the skull deformity even within the same etiology. The ability to apply the simulation surgery for improving the preoperative planning for distraction osteogensis could improve the results.Planning and Simulation 14 patients presented with craniosynostosis had been subjected to simulation surgery prior to real surgery. 3D CT scans was obtained upon patient admission. Adjustment of all skull position to Frankfort horizontal plane was done. 3 different distraction osteogensis plans were done for each patient according to the skull morphology. For each plane, movement for each bone segment was done according to the pre-planned distraction vectors. Also the distances of distractions were pre-determined according to the cephalic index as well as brain volume. Intraoperatively, we choose the most appropriate plan for the patient by the surgeon. At the end of distraction, 3D CT scan was obtained, and was compared to the simulation plan. Also the distance and the direction of distraction was compared to that of the plan. Accordingly, the distance was almost matching that of the simulation surgery, however the vector of distraction was not matched.Conclusion Preoperative stimulation planning for craniosynostosis patient is very valuable tool in the surgical management of craniosynostosis patients.

• The Korean Journal of Pain
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• 제18권1호
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• pp.85-88
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• 2005

Encephalitis is known as a rare complication of varicella zoster virus (VZV) reactivation. It is usually regarded as a complication of a cutaneous infection in patients with impaired cellular immunity. The reported incidence of herpetic motor involvement range between 0.5 and 31%, but is possibly more frequent as the weakness is readily obscured by pain. A 53-years-old woman, who presented with severe shoulder pain, fever, headache and seizure, which developed the day after skin eruptions, also developed motor paresis 7 days after the seizure. Her cerebrospinal fluid (CSF) was VZV-Polymerase chain reaction (PCR) negative, but VZV specific IgG antibody positive, and her brain MRI was found to be normal. With the early diagnosis and proper treatment, such as intravenous administration of acyclovir, stellate ganglion block and Yamamoto New Scalp Stimulation (YNSS), the patient completely recovered, without psychoneurological sequelae. Herein, we present this case, with a discussion of the relevant literature on the incidence, pathophysiology, diagnosis and management of central nervous system VZV involvement.

• 대한의용생체공학회:학술대회논문집
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• 대한의용생체공학회 1996년도 춘계학술대회
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• pp.14-17
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• 1996

Evoked Potentials signals occur as a result of neuroelectric responses of the brain to sensory stimulation. In this paper, to analysis such signals we utilize a time-frequency analysis technique called wavelet transform. The wavelet analysis is performed based on a single prototype function,which can be thought of as a bandpass filter. Because the wavelet transform in a fine temporal analysis decomposes time-varying signals in EP into a dilated lowpass and a contracted highpass components, EP signal fetures can be obtained and analysed quantitatively at the levels of resolution. In the results, we analyze the VEP signal with the wavelet transform.

• Clinical and Experimental Pediatrics
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• 제63권11호
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• pp.422-428
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• 2020

In today's world, most children are exposed to various manmade electromagnetic fields (EMFs). EMFs are electromagnetic waves less than 300 GHz. A developing child's brain is vulnerable to electromagnetic radiation; thus, their caregivers' concerns about the health effects of EMFs are increasing. EMF exposure is divided into 2 categories: extremely low frequencies (ELFs; 3-3,000 Hz), involving high-voltage transmission lines and in-house wiring; and radiofrequencies (RFs; 30 kHz to 300 GHz), involving mobile phones, smart devices, base stations, WiFi, and 5G technologies. The biological effects of EMFs on humans include stimulation, thermal, and nonthermal, the latter of which is the least known. Among the various health issues related to EMFs, the most important issue is human carcinogenicity. According to the International Agency for Research on Cancer's (IARC's) evaluation of carcinogenic risks to humans, ELFs and RFs were evaluated as possible human carcinogens (Group 2B). However, the World Health Organization's (WHO's) view of EMFs remains undetermined. This article reviews the current knowledge of EMF exposure on humans, specifically children. EMF exposure sources, biological effects, current WHO and IARC opinions on carcinogenicity, and effects of EMF exposures on children will be discussed. As well-controlled EMF experiments in children are nearly impossible, scientific knowledge should be interpreted objectively. Precautionary approaches are recommended for children until the potential health effects of EMF are confirmed.

• 대한의생명과학회지
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• 제25권2호
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• pp.139-148
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• 2019

The role of electroencephalography (EEG) in the development of brain-computer interface (BCI) technology is increasing. In particular, the importance of the analysis of event related potential (ERP) in various situations is becoming more significant in BCI technology. Studies on past maze and fire situations have been difficult due to risks and realistic problems. Nowadays, according to the development of virtual reality (VR) technology, realistic maze and fire situation can be realized. In this study, ERPs (P300, and evented related negativity) were analyzed to collect objective data on case determination in an emergency situation. In order to overcome the limitations of previous methods that evaluating the EEG frequency change, ERPs were derived by setting epochs for stimulation and standardizing them, and evaluated for ERPs in this study. P3a and P3b, which are subcomponents of P300, were analyzed and the evented related negativity (ERN) was analyzed together with error positivity (Pe). As a result of the study, statistically significant changes of ERPs were observed, this result, which has little related research, is considered to be meaningful as medical basic statistics.

• The Journal of Korean Physical Therapy
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• 제34권3호
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• pp.98-103
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• 2022

Purpose: New therapeutic approaches have emerged to improve gait ability in patients with brain damage, such as action observation learning (AOT), auditory cueing, motor imagery etc. We attempted to investigate the effects of AOT with auditory cueing (AOTAC) on gait function in patients with stroke. Methods: The eighteen stroke patients with a unilateral hemiparesis were randomly divided into three groups; the AOTAC, AOT, and control groups. The AOTAC group (n=8) received training via observing a video that showed normal gait with sound of footsteps as an auditory cue; the AOT group (n=6) receive action observation without auditory stimulation; the control group (n=5) observed the landscape video image. Intervention time of three groups was 30 minutes per day, five times a week, for four weeks. Gait parameters, such as cadence, velocity, stride length, stance phase, and swing phase were collected in all patients before and after each training session. Results: Significant differences were observed among the three groups with respect to the parameters, such as cadence, velocity, stride length, and stance/swing phase. Post-hoc analysis indicated that the AOTAC group had a greater significant change in all of parameters, compared with the AOT and control groups. Conclusion: Our findings suggest that AOTAC may be an effective therapeutic approach to improve gait symmetry and function in patients with stroke. We believe that this effect is attributable to the change of cortical excitability on motor related to cortical areas.