• Journal of Microbiology and Biotechnology
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• 제11권5호
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• pp.776-780
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• 2001

Based on the static and dynamic adsorption of Streamline DEAE, a modified tank-in-series model including particle size distribution was used to describe the adsorption performance of bovine serum albumin in an expanded bed. The calculated results indicated that the suggested model was able to simulate breakthrough curves under various conditions.

• Archives of Pharmacal Research
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• 제4권1호
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• pp.19-24
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• 1981

The possibility of using a fluorescence probe technique for the study of ibuprofenlysine binding to human and bovine serum albumin was investigated. 1-anilino-8-naphalenesulfonate was used as the probe. The number of binding sites of human and bovine serum albumins for ibuprofenlysine appears to be 4 and 2, respectively. By using this technique, the association constants were found to be $1.533{\times}10^{4}M^{-1}$ and $2.238{\times}10^{4}M^{-1}$, respectively.

• 한국임상수의학회지
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• 제20권4호
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• pp.431-436
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• 2003

이 연구의 목적은 실험적으로 유발한 면역매개성 사구체 질환이 신증후군으로 진행하는 과정과 치유과정에서 단백뇨 배설에 근거한 조기진단 및 치유과정의 감시에 대한 평가를 하는 것이다. 두당 endotoxin 1 $\mu$g과 native bovine serum albumin 5mg 을 9두의 건강한 개에 정맥주사하여 감작시키고, 그 후 1주일부터 양이온화한 bovine serum albumin 120mg을 주 5회 반복 정맥주사한 결과 5두에서 면역매개성 사구체신염과 신증후군이 발생하였다. 사구체신염이 발생한 실험동물에서는 요단백질/ 크레아티닌 비의 증가 (>1.0), 저알부민혈증 (< g/이), BUN 증가 (>40 mg/dl), 부종 등이 나타났다. 이것은 임상병리학적으로 평가 가능한 면역매개성 사구체시염 및 신증후군 모델견이 제작되었음을 제시하는 것이었다. 사구체신염의 유발과 신증후군의 진행과정에 요단백질/크레아티닌 비의 증가가 저알부민혈증, BUN 증가, 고콜레스테롤혈증보다 1-4주 먼저 출현하였다. 사구체신염에 의해 신증후군이 유발된 실험동물 모두에서 prednisolone 을 2.2 mg/kg씩 1일 2회 경구투여한 결과 혈중 알부민 농도의 증가와 콜레스테롤 농도의 감소가 먼저 일어나고, 그 후 1-4 주에 요단백질/크레아티닌 비가 감소하기 시작하였다. 종합적으로 볼때, 면역매개성 사구체신염성 신증후군의 조기진단에는 요 단백질/크레아티닌 비가 유리한 지표로, 그리고 초기 치유과정의 감시에는 혈청 알부민, 콜레스테롤이 더 유리한 임상 지표로 이용될 수 있음을 확인하였다.

• 대한수의학회지
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• 제29권1호
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• pp.57-60
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• 1989

This study was carried out to evaluate the possibility of sex preselection by gradients methods using bovine serum albumin in rabbits. Artificial insemination was performed with sperm from the top and bottom layer of rabbit semen separated by bovine serum albumin gradients. Various characteristics of separated sperm, and the conception rate and secondary sex ratio at artificial insemination with separated sperm were compared. The results obtained were as follows. 1. The sperm from the bottom layer showed significanty high value in motility, percent of normal sperm and progressive motility as compared with control sperm and the sperm and the sperm from the top layer. 2. The conception rate of sperm from the bottom layer was higher than that of the top layer. But secondary sex ratio was not altered by this methods.

• 대한한의학회지
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• 제30권4호
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• pp.93-107
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• 2009

Objective: Membranous nephropathy (MN) is one of the most common causes of nephrotic syndrome in adults. However, there is not a satisfactory treatment for MN. This study aimed to evaluate the effect of Houttuyniae Herba Extract (HHE) on MN induced by cationic bovine serum albumin (cBSA). Methods: Mice were divided into 4 groups. The first group, Normal, was injected with saline. The second group, Control, was treated with cBSA (10mg/kg i.p) only. The third group, HHE-250, was treated with cBSA (10mg/kg i.p) and HHE (250mg/kg, p.o). The fourth group, HHE-500, was treated with cBSA (10mg/kg i.p) and HHE (500mg/kg, p.o). After treatment for 4 weeks, we measured change of body weight, 24 hrs proteinuria, serum albumin, total cholesterol, triglyceride, BUN, creatinine, IgA, IgM, IgG, TNF-${\alpha}$, IL-1${\beta}$ levels and the mRNA expression of IFN-${\gamma}$, IL-6, and IL-10. The morphologic changes of renal glomeruli were also observed with a light microscope and an electron microscope. Results: The levels of 24 hrs proteinuria and serum triglyceride, BUN, IgG, TNF-${\alpha}$, IL-1${\beta}$ significantly decreased in both HHE groups, while the level of serum albumin significantly increased in both HHE groups. The mRNA expression of IFN-${\gamma}$ and IL-6 in splenocytes considerably increased in both HHE groups. The mRNA expression of IL-10 in splenocytes considerably decreased in both HHE groups. In histological findings of kidney tissue, thickening of GBM decreased in both HHE groups. Conclusions: This study shows that HHE might be effective for treatment of acute stage MN. More clinical data and studies are to be done for efficient application.

• 대한수의학회지
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• 제8권1호
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• pp.45-53
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• 1968

1. The albumin, ${\beta}-globurin$ and ${\gamma}-globurin$ fractions of non-vaccinated bovine serum (Control) int declined, and a total protein and ${\alpha}-globurin$ level are advanced on the reversible rather than of vaccinated immumized bovine serum. (Table 5.) 2. Some few exists the to bring about changed in the individuals and sexual in a vaccinated bovine serum, however, Male serum globurin fractions are higher than Female globurin fractions percentage. (Table 1 and 3.) 3. Albumin fractions are Age-ablly variable, so that, younger's are rather lower than adult's such reported as in the another litratures. 4. In the monthly analysis of immunized bovine serum the first week to at dulation for third weeks were slowly advanced as variablly in serum-protein fractions of ater by the Black-leg No. 2 vaccination, then, albumin fractions were illustated as maximum ratio (42.73 3.49%) and increased much as 14.9% more than non-vaccinates, and ${\alpha}-globurin$ fractions indicated the minimum ratio(15.11 2.35%) at for 4th month after vaccination decrease much as about 7.71 % rather non-vaccinated normal bovine serum. (Table 4. and Fig 3.) Next, continuous advanced the ${\beta}-globurin$ fractions at first month as primary crisis in a diagram, and indicated the maximum ratio at 8th months as the second crieir on the its diagram of after vaccinati on, however, few changed in non-vaccinated bovine serum. (Figs 4.) Especially, ${\gamma}-globurin$ fractions are advanced the maximum ratio as 41. 45% 4.48% anp advanced to be widely range much as about 22.55% more than control serum at 5th months of after the vaccination. That is one of the most considerable evalution in Black-leg No. 2 vaccination to Korean calevs as great presence of the maximum immune antibodies at for 5th month after the vaccination. (Table 4. and Fig 4.) 5. In the relationship between vaccinated rabbit and Korean calves, serum protein fraractions were to be changed within the 3 weeks, so that albumin fractions of vaccinated immune bovine serum are increased as directly ratio, while vaccinated rabbit immunized serum showed the decrease as to reciprocal ratio. Although, conclude that ${\gamma}-globurin$ fractions are increased gradually by and large on the vaccinated immune bovine serum(B. P. S.) and vaccinated immune rabbit serum(R. P. S.) together. (Table 6).

• 대한화학회지
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• 제36권2호
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• pp.329-334
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• 1992

단백질 수용액 중의 초음파흡수 mechanism을 규명하기 위하여 pH 7의 소혈청 albumin(BSA)수용액의 초음파흡수 측정을 주파수 100 kHz에서 1600 MHz에 걸쳐 행하였다. 측정방법은 plano-concave 공명법(0.1${\sim}$10 MHz)과 고분해능 Bragg 반사법(120${\sim}$1600 MHz)을 사용하였다. pH7에서의 초음파흡수스펙트럼은 Davidson-Cole 분포식의 거울상인 분포함수를 사용한 완화곡선과 일치하였다. 이 완화현상을 BSA 분자의 수화평행에 의한 것으로 해석하였다.

• Archives of Pharmacal Research
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• 제6권1호
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• pp.55-62
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• 1983

Binding of six cephalosporins (cefotaxime, cefuroxime, cefazoline, cephalothin, cephaloridine, cephacetrile) to bovine serum albumin was studied. Fluorescence probe technique and difference spectrophotometry were employed to evaluate the nature and degree of association of cephalosporin albumin complex. 1-Anilinonaphthalene-8-sulfonate (ANS) was used as the fluorescence probe. 2-(4'-hydroxybenzeneazo) benzoic acid(HBAB) was employed as the UV spectrophotometric probe. Compentitive bindings between cephalosporins and probes were observed. The number of binding sites of bovine serum albumin for each cephalcsporin is 2. Among six cephaloporins, cefotaxime has the highest binding constant followed by cafazoline, cefuroxime, cephalothin, cephaloridine and cephacetrile.

• 공업화학
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• 제9권2호
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• pp.311-316
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• 1998

물리 화학적인 특성이 다른 다공성 고분자 마이크로겔에 대한 bovine serum albumin (BSA-protein) 단백질의 흡착평형 특성을 연구하였다. 수용액 속에서 고분자와 단백질사이의 소수성 상호작용에 의하여 폴리부틸메타크릴레이트 (PBMA) 마이크로겔이 폴리비닐피리딘 (PVP)과 폴리아크릴로니트릴 (PAN) 마이크로겔보다 높은 흡착특성을 나타내었으며, PBMA 마이크로겔이 PVP와 PAN 마이크로겔보다 비가역적으로 흡착평형 특성을 나타내었다. 그러므로 고분자 마이크로겔의 물리적인 특성과 단백질-고분자 마이크로겔 사이의 정전기적 인력보다는 소수성 상호작용이 단백질의 흡착특성에 중요한 역할을 하고 있음을 알 수 있다. 또한 PBMA, PVP 및 PAN 마이크로겔 모두 Freundlich 흡착 등온식보다는 Langmuir 흡착 등온식에 잘 적용되었다.

• 농업과학연구
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• 제39권4호
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• pp.561-568
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• 2012

The aim of this study was to introduce a simple method for isolation of ${\alpha}$-lactalbumin, ${\beta}$-lactoglobulin and bovine serum albumin from cow's milk, and peptides produced by enzymatic hydrolysis of ${\alpha}$-lactalbumin, ${\beta}$-lactoglobulin and bovine serum albumin with alcalase. Whey protein were precipitated from whey by ammonium sulfate and, ${\alpha}$-lactalbumin and ${\beta}$-lactoglobulin were isolated using Hi Prep 26/60 Sephacryl S-100 column gel filtration chromatography. Bovine serum albumin and ${\beta}$-lactoglobulin were isolated by Mono-Q 5/50 GL column anion exchange chromatography of the 50% Ammonium Sulfate-supernatant. Isolated whey proteins were hydrolyzed by proteolytic alcalase. Tricine SDS-PAGE and reverse-phase HPLC analyses revealed that almost hydrolyzed all the ${\alpha}$-lactalbumin, ${\beta}$-lactoglobulin and bovine serum albumin with alcalase. Molecular weight of various peptides derived from alcalase hydrolysate were small molecular weight than 3.5 kDa.