• 지구물리와물리탐사
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• 제10권2호
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• pp.131-137
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• 2007

초동발췌 방법과 이와 관련된 오차문제를 고찰하고 자료처리 전산화 측면에서 초동발췌 및 오차계산의 실용적 알고리즘을 제안하였다. 제안한 초동발췌 방법은 2단계로 구성된다. 1) 초동신호 중 트레이스 간 연속성이 양호한 최초의 피크(peak) 또는 트라프(trough)를 발췌한다. 2) 발췌시점 전방 일정구간의 기록을 직선으로 근사화 하고 이 직선의 시간 절편을 초동주시로 한다. 근사화 구간의 길이는 대략 초동 웨이브렛 폭의 1/4보다 다소 작게 설정한다. 초동발췌에 내재된 오차의 정량적인 척도는 기록의 특정시점에 초동의 도착 여부를 판단하는 데 필요한 기록의 길이로 정의한다. 기록의 길이를 나타내는 공식은 신호의 주파수대역과 신호대잡음비의 함수로 표시된다. 3개의 공대공 탄성파기록의 초동을 수동 및 제안한 방법으로 발췌하였고 각 트레이스의 오차한계를 계산하였다. 실험결과 제안한 근사직선 시간절편법의 우수한 성능과 발췌된 초동평가에 있어서 발췌오차의 유용성을 확인하였다.

• Bulletin of the Korean Chemical Society
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• 제30권11호
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• pp.2549-2554
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• 2009

The structures of 1,n-alkanedithiol (n = 2, 4, 6, 8, 10) self-assembled monolayers (SAMs) on a Au(111) substrate were investigated by electrochemical measurements and theoretical calculations. The results of the experimental techniques indicated that the dithiols, except n = 2, showed an upright molecular structure in the SAMs, in which alkanedithiols were bound to the Au surface via only one thiol functionality and the other one faced up to the air. The results also suggested that the formed dithiol SAMs were densely packed and highly oriented. Except ethanedithiol, which was thought to form a bilayer, the reductive desorption peak potentials of 1,n-alkanedithiol (n = 4, 6, 8, 10) SAMs were more negative than those of the corresponding monothiol ones in 0.1 M KOH solutions. This illustrates that the dithiol SAMs had higher stability than the corresponding monothiol ones. The major part of the high stability may be attributed to the van der Waals interaction among the sulfur atoms on top of the dithiol SAMs. The molecular modeling calculation showed that the structures of dithiol SAMs were similar to those of the corresponding monothiol SAMs and that all the dithiol SAMs, except ethanedithiol, were more stable than the corresponding monothiol SAMs. The calculated energy differences between dithiol and monothiol SAMs decreased with the increment of alkyl-chain length.

• 한국공간구조학회논문집
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• 제4권4호
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• pp.113-128
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• 2004

A three-dimensional (3-D) method of analysis is presented for determining the free vibration frequencies and mode shapes of solid paraboloidal and complete (that is, without a top opening) paraboloidal shells of revolution with variable wall thickness. Unlike conventional shell theories, which are mathematically two-dimensional (2-D), the present method is based upon the 3-D dynamic equations of elasticity. The ends of the shell may be free or may be subjected to any degree of constraint. Displacement components $u_r,\;u_{\theta},\;and\;u_z$ in the radial, circumferential, and axial directions, respectively, are taken to be sinusoidal in time, periodic in ${\theta}$, and algebraic polynomials in the r and z directions. Potential (strain) and kinetic energies of the paraboloidal shells of revolution are formulated, and the Ritz method is used to solve the eigenvalue problem, thus yielding upper bound values of the frequencies by minimizing the frequencies. As the degree of the polynomials is increased, frequencies converge to the exact values. Convergence to four digit exactitude is demonstrated for the first five frequencies of the complete, shallow and deep paraboloidal shells of revolution with variable thickness. Numerical results are presented for a variety of paraboloidal shells having uniform or variable thickness, and being either shallow or deep. Frequencies for five solid paraboloids of different depth are also given. Comparisons are made between the frequencies from the present 3-D Ritz method and a 2-D thin shell theory.

• Animal cells and systems
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• 제9권3호
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• pp.161-171
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• 2005

Integrin-linked kinase 1 (ILK1) regulates several protein kinases, including PKB/Akt kinase and glycogen synthase kinase ${\beta}$. ILK1 is also involved distinctively in the cell morphological and structural functions by interacting with the components of the extracellular matrix or integrin. According to the information of serum- and glucocorticoid-induced kinase 1 (SGK1) substrate specificity (R-X-R-X-X(S/T)-${\phi};{\phi}$ indicates a hydrophobic amino acid), two putative phosphorylation sites, $Thr^{181}\;and\;Ser^{246}$, were found in ILK1. We showed that ILK1 fusion protein and two fluorescein-labeled ILK1 peptides, $FITC-^{174}RTRPRNGTLN^{183}$ and $FITC-^{239}CPRLRIFSHP^{248}$, were phosphorylated by SGK1 in vitro. We also identified that 14-3-3 ${\theta}\;{\varepsilon}\;and\;{\xi}$, among several 143-3 isotypes $({\beta},\;{\gamma},\;{\varepsilon},\;{\eta},\;{\sigma},\;{\theta},\;{\tau}\;and\;{\xi})$ formed protein complex with ILK1 in COS-1 cells. Furthermore, the phosphorylation of $Ser^{246}$ by SGK1 induced the binding with 14-3-3. It was also demonstrated that 14-3-3-bound ILK1 has reduced kinase activity. Thus, these data suggest that SGK1 phosphorylates $Thr^{181}\;and\;Ser^{246}$ of ILK1 and the phosphorylation of its $Ser^{246}$ makes ILK1 bind to 14-3-3, resulting in the inhibition of ILK1 kinase activity.

• 한국식품과학회지
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• 제37권2호
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• pp.164-170
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• 2005

미생물유래의 중합화효소(microbial transglutaminase, MTGase)를 이용하면 식품 단백질의 기능특성을 향상시키는 것이 가능하다. 본 연구에서는 MTGase를 이용, 동물성 단백질인 ${\beta}$-casein 및 식물성 단백질인 11S globulin의 동종 혹은 이종간을 bio-hybrid시킴으로써 단백질의 기능특성을 향상시키고자 하였다. 즉, SDS-PAGE에 의한 susceptibility 확인, particle size 분석 및 Reddy and Foster 방법에 의한 유화안정성(emulsion stability) 실험, 그리고 유화안정성에 영향을 주는 bio-hybrid된 단백질이 어떤 형태로서 안정성에 영향을 미치는지 주사형 전자현미경(SEM)을 이용하여 유적(oil droplet)에 결합된 단백질을 고배율로 분석하였다. SDS-PAGE에 의해 동종 흑은 이종의 단백질간의 가교결합이 형성되었으며, 특히 이종간의 결합은 그 밴드가 약하게 형성되었으며 oligomer의 형태를 나타내었다. 또한 유화안정성은 동종간에서는 ${\beta}$-casein이 우수하였으며, glycinin은 안정성이 거의 없는 것으로 나타났다. 그러나, 두 단백질 이종간의 안정성은 glycinin보다 더 우수하였다. 전자현미경 사진에서는 유적에 bio-hybrid된 단백질이 고르게 분포될수록 안정성이 더 우수한 것으로 판단되었다.

• 생명과학회지
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• 제19권7호
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• pp.859-865
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• 2009

미세소관은 세포골격단백질의 중요한 구성 단백질로 축삭돌기 내에서는 세포막 방향으로 정렬되어 있다. Kinesin superfamily (KIFs)는 세포 내에서 미세소관을 따라 세포 내 소포들을 운반하는 분자 자동차 (molecular motor) 단백질이다. 본 연구에서 우리는 효모 two-hybrid system을 사용하여 KIF1A의 coiled-coil 영역과 결합하는 단백질로 미세소관 불안정화 요소인 SCG10 단백질을 분리하였다. SCG10은 KIFs에서 KIF1A와만 특이적으로 결한 하며, KIF1A의 400에서 820아미노산 부위가 SCG10과의 결합에 필수적임을 효모 two-hybrid assay로 확인하였다. 또한 SCG10의 coiled-coil영역은 KIF1A와의 결합에 필수영역임을 확인하였으며 단백질간의 결합은 Glutathione S-transferase pull-down assay를 통하여 확인하였다. 생쥐의 뇌 파쇄액에 SCG10항체로 면역침강을 행하여 KIF1A를 확인한 결과KIF1A는 SCG10과 특이적으로 같이 침강하였다. 이러한 결과들은 KIF1A는 SCG10와 결합하여 SCG10이 포함된 소포를 미세소관을 따라 이동시킴을 시사한다.

• 한국실내디자인학회논문집
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• 제18권6호
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• pp.96-103
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• 2009

Man has an unconscious desire to free oneself from everyday selves and a strong intellectual desire to see and analyze things in a different perspective. This provided a momentum for architectural design that has long been confined to its limits of modernism of being rational and objective. The ever-changing society that we live in filled with various attempts and pluralistic characteristics shows a complex set of attributes that can hardly be defined by a single term. As society moves towards an uncertain and volatile state, the boundary between literature, philosophy and art has dimmed out and converged. Such tendencies have been observable in recent establishments in architecture in the form of introducing external elements and create an alien and farfetched architectural space. Using the 'depaysement' defamiliarization method expressing irrationality and randomness, it is applied in various aspects, sharing the counter-rational structure and features of such architectural space. Hence, it is the purpose of this study to delve into the unique characteristics of depaysement through Magritte's work which reflects the unique pictorial world built under the influence of surrealism, and discover the $D\acute{e}paysement$ tendencies in modern architectural space. It is apparent in Modern architecture that, owing to the development in digital technology, it is showing process changes in design and an interfacial $D\acute{e}paysement$ effect in cyberspace, an interlace of reality and the virtual world. In this study, the focus of analysis was architectural properties such as the program, figure/spatial, material and the objective side, but due to mutual text properties, it is bound to be an overlapping analysis.

• 정보보호학회논문지
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• 제26권5호
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• pp.1089-1097
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• 2016

RSA 시스템에서 암 복호문 이외의 부가 정보가 주어졌을 때 개인키를 알아내는 것은 소인수분해보다 더 쉬울 수 있음이 잘 알려져 있다. 예를 들어, Coppersmith는 RSA 시스템을 구성하는 소수 중 하나의 최상위 또는 최하위 비트의 절반 이상이 주어지면 RSA 모듈러스가 다항식 시간 안에 인수분해될 수 있음을 보였다. 또한 Henecka 등은(p, q, d, $d_p$, $d_q$) 형태의 RSA 개인키 비트 중 23.7%에 해당하는 비트에 에러가 삽입되더라도 원래의 RSA 개인키를 복구할 수 있는 알고리즘을 제안하였고, 이를 위해 후보 키 비트와 에러가 삽입된 RSA 개인키 비트 사이의 서로 매칭이 되는 비트들의 개수를 사용할 것을 제안하였다. 본 논문에서는 Henecka 등의 방법을 확장하여, 후보 키 비트와 에러가 삽입된 개인키 비트 사이의 일치되는 정도를 보여주는 좀 더 일반화된 확률 측도의 사용과 이 측도를 사용한 RSA 개인키 복구 알고리즘을 제시한다.

• Biomolecules & Therapeutics
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• 제25권2호
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• pp.171-176
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• 2017

Streptomyces avermitilis produces clinically useful drugs such as avermectins and oligomycins. Its genome contains approximately 33 cytochrome P450 genes and they seem to play important roles in the biosynthesis of many secondary metabolites. The SAV_7130 gene from S. avermitilis encodes CYP158A3. The amino acid sequence of this enzyme has high similarity with that of CYP158A2, a biflaviolin synthase from S. coelicolor A3(2). Recombinant S. avermitilis CYP158A3 was heterologously expressed and purified. It exhibited the typical P450 Soret peak at 447 nm in the reduced CO-bound form. Type I binding spectral changes were observed when CYP158A3 was titrated with myristic acid; however, no oxidative product was formed. An analog of flaviolin, 2-hydroxynaphthoquinone (2-OH NQ) displayed similar type I binding upon titration with purified CYP158A3. It underwent an enzymatic reaction forming dimerized product. A homology model of CYP158A3 was superimposed with the structure of CYP158A2, and the majority of structural elements aligned. These results suggest that CYP158A3 might be an orthologue of biflaviolin synthase, catalyzing C-C coupling reactions during pigment biosynthesis in S. avermitilis.

• Plant Biotechnology Reports
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• 제2권1호
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• pp.21-31
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• 2008

In plants, the oxygen generated by photosynthesis can be excited to form reactive oxygen species (ROS) under excessive sunlight. Excess ROS including singlet oxygen ($^1O_2$) inhibit the growth, development and photosynthesis of plants. To isolate ROS-resistant crop plants, we used paraquat (PQ), a generator of $O_2{^-}$ as a source of screening and mutagen, and obtained two PQ-resistant lines in Pisum sativum, namely R3-1 and R3-2. Both lines showed greater resistance to PQ than their wild type (WT) siblings with respect to germination, root growth, and shoot growth. Biochemical analysis showed differences in these lines, in which ROS-scavenging enzymes undergo changes with a distinguishable increase in Mn-SOD. We further observed that the cytosolic catalases (CATs) in leaves in both lines were shifted in a native-PAGE analysis compared with that of the WT, indicating that the release of bound $^1O_2$ was enhanced. Phenotypic analysis revealed distinguishable differences in leaf development, and in flowering time and position. In addition, R3-1 and R3-2 showed shorter individual inter-node lengths, dwarf plant height, and stronger branching compared with the WT. These results suggested that PQ-induced ROS-resistant Pisum have the potential pleiotropic effects on flowering time and stem branching, and that ROS including $^1O_2$ plays not only important roles in plant growth and development as a signal transducer, but also appears as a strong inhibitor for crop yield.