• Title/Summary/Keyword: bottle gourd

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Absence of AVP1 transcripts in wild type watermelon scions grafted onto transgenic bottle gourd rootstocks

  • Kim, Byung Oh;Han, Jeung-Sul;Park, Kyung Il;Jeon, Su Min;Kim, Chang Kil
    • Journal of Plant Biotechnology
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    • v.42 no.1
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    • pp.13-18
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    • 2015
  • In this study we confirmed the stable integration of Arabidopsis AVP1 in the genomes of bottle gourd $T_3$ homozygous lines and its transcription, and additionally evaluated possibility of translocation of the AVP1 mRNA from transgenic bottle gourd rootstocks to wild type watermelon scions. Each AVP1 gene in two bottle gourd T3 lines is abundantly expressed under a field condition. Given the grafting between wild type watermelon scions and AVP1-expressing bottle gourd rootstocks, no translocation of the AVP1 mRNA was detected in leaves, both sexual flowers, and fruits of the scions.

Diversity and origin of bottle gourd, Lagenaria

  • Yuasa, Hiroshi
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2002.11a
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    • pp.96-97
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    • 2002
  • Bottle gourd, Lagenaria siceraria, is one of the oldest cultivated plants. To bigin with, its fruit was used as a complete liquid bottle or container. It was a very widespread cultivated plant in prehistoric times, for example (there) is a report from Peru as early as between 13,000 B.C and 11,000 B.C. The dug-out finds in Japan proved to be about 95,000 years old according to the /sup 14/C analysis. The bottle grourd was the most important plant before the invention of pottery in many areas of Asia, New Guinea, Polynesia, America, and Africa. I would like to suggest that there should be "The Bottle Gourd Age" prior to the Pottery Age. Bottle gourds are also used for various purposes such as food, masks, pipes, musical instruments, medicine, symbols, artistic products and also as penis-sheaths of men's attire. Their purposes number more than 220 including 70 varienties of containers or bottles. I consider that its utilization should be called a culture, as it were, "The gourd culture." The shape and the size of the fruit of bottle gourd have a larger variety than those of any other plant. As for the size, it is reported that they range from those that are shorter than 3cm to those that are ovoid and longer than 60cm in diameter. With regard to the shape of its fruit, the bottle gourd can be classified into 7 groups and even more than 30 races, considering the difference of the size. The seeds are so variable without two horn-like projections, with smooth surface or longitudinalines, white or brown, with smooth corky margin. Generally, it seems that there is no correlation between seem shape and fruit shape. My study shows that the seeds of gourd. My study shows that the seeds of gourd in Asia are so simple in shape and in color except for the size. But the seeds of those in Africa are various and seem to be beyond the confines. Explaining the great diversity of the seeds of those in Africa therefore, they appear to have no correlation among the types of fruit of the bottle gourd with African origin. It might be supposed that another 4 wild Lagenaria spp. distribute only in Africa. The intraspecies hybrid is confirmed between the bottle gourd, Lagenaria siceraria, and wild L. spehaerica. And Fl hybrid is sterile: it has no fertility. However, even though the shapes of the fruit and the seeds are different, they can cross with each other. Moreover, their hybrids grow up to be extremely vigorous and have normal fertility. Fruit shapes of Fl plants are similar to those of their parents, if they have the same character. Whereas, the fruit shape often became different from each other when the cross occurs between those with different fruit shapes. It is shown that dumbbell shapes, HYOTAN in Japanese, is recessive while its bitterness is dominant. Thus the character of F2 hybrids Is segregative. I'll give further particulars of the heredity of the groud in my lecture.

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Cultivation and use of bottle gourd (Lagenaria siceraria) in ancient Korea (한반도 선·역사시대 박의 재배와 이용)

  • KIM Sebin;KIM Minkoo
    • Korean Journal of Heritage: History & Science
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    • v.57 no.1
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    • pp.38-51
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    • 2024
  • Although the bottle gourd (Lagenaria siceraria) is a crop with a cultivation history of about 10,000 years in the Old and New Worlds, archaeological considerations on the cultivation and use of bottle gourds on the Korean Peninsula are extremely rare. Accordingly, we reviewed previous reports on bottle gourds and examined the morphological characteristics of seeds and rind fragments from the Korean Peninsula. The investigation yielded several conclusions. First, bottle gourd cultivation likely began during the Bronze Age alongside the introduction of so-called southern crops. Evidence suggests that bottle gourd remains were more prevalent during the Three-Kingdoms period, indicating its significance as a crop during the historical era. Second, bottle gourd seeds from the Three-Kingdoms period exhibit characteristics of both African and Asian subspecies, showcasing a high level of morphological diversity. Third, rind thickness indicates that bottle gourds found at the Bongseon-ri site were of varieties with large fruits. Taken together, it is concluded that the bottle gourd was introduced to the Korean Peninsula during the Bronze Age, and people cultivated a range of bottle gourd varieties during the Three-Kingdoms period.

Diversity and origin of bottle gourd, Lagenaria

  • Yuasa, Hiroshi
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2002.11b
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    • pp.13-14
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    • 2002
  • Bottle gourd, Lagenaria siceraria, is one of the oldest cultivated plants. To bigin with, its fruit was used as a complete liquid bottle or container. It was a very widespread cultivated plant in prehistoric times, for example (there) is a report from Peru as early as between 13,000 B.C and 11,000 B.C. The dug-out finds in Japan proved to be about 95,000 years old according to the $^{14}$ C analysis. The bottle grourd was the most important plant before the invention of pottery in many areas of Asia, New Guinea, Polynesia, America, and Africa. I would like to suggest that there should be “The Bottle Gourd Age” prior to the Pottery Age.(중략)

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Root Rot of Bottle Gourd Stock of Watermelon Caused by Monosporascus cannonballus in Korea (수박 대목용 참박에 발생한 Monosporascus cannonballus에 의한 검은점뿌리썩음병(黑点根腐病))

  • 박경석;남상현;김충회
    • Korean Journal Plant Pathology
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    • v.10 no.3
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    • pp.175-180
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    • 1994
  • The fungal pathogen Monosporascus annonballus was first isolated in Korea from the rotted roots of bottle gourd stocks of collapsed watermelon plants in fields near Chochiwon, Choongnam province in July, 1993. Perithecia of M. cannonballus were dark brown to black, globose, 220~570 ${\mu}{\textrm}{m}$ in diam. and had many asci. Asci are hyaline, clavate to pyriform, and 50~120$\times$35~570 ${\mu}{\textrm}{m}$ in size. Ascospores were aseptate, dark brown to black, globose, 25~45$\times$30~50 ${\mu}{\textrm}{m}$ in diam, and borne singly in each ascus. The fungus grew in the temperature range of 4 to 34$^{\circ}C$, best at 3$0^{\circ}C$. The optimum pH for growth was 6.8. Mycelial growth rate of M. cannonballus was 25.5mm/day on PDA at 26$^{\circ}C$. Perithecia began to form after 20-day-growth on PDA and produced mature asci after 30 days or later. In the greenhouse inoculation tests, the fungus developed water-soaked lesions on roots of bottle gourd seedlings and was then reisolated from the lesions. Severed damages on watermelon plants by M. cannonballus are greatly concerned in Korea, since no stocks used for watermelon cultivation have reported to be resistant to the fungus.

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Effect of Low-Dose Gamma Radiation to Enhance Germination Rate in Bottle Gourd and Pumpkin Seeds (참박과 호박 종자의 발아촉진을 위한 저선량 감마선의 효과)

  • Kim, Jae-Sung;Baek, Myung-Hwa;Lee, Young-Keun;Lee, Hae-Youn;Yoo, Jun-Cheol
    • Korean Journal of Environmental Agriculture
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    • v.21 no.3
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    • pp.202-207
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    • 2002
  • To establish the seed treatment method of the gamma radiation for germination enhancement of bottle gourd(Lagenaria siceraria Standl.) and pumpkin(Cucurbita ficifolia Bouche), seeds were irradiated with the dose of 1$\sim$20 Gy of the gamma radiation. The germination rate in irradiated group was higher than that of the control, depending on seed condition. In the case of bottle gourd, the germination rate of 70$\sim$80% germinative seed was increased at 4$\sim$20 Gy irradiated groups. Especially, it was the highest at 4 Gy irradiated group. The germination rate of 80$\sim$90% germinative seed was the highest at 4 Gy irradiated group. The germination rate of 70$\sim$80% and 80$\sim$90% germinative seed at 4 days after sowing was increased 66% and 26% at 4 Gy irradiated group, respectively. In the case of pumpkin, the germination rate of 70$\sim$80% and 80$\sim$90% germinative seed was increased at 2 Gy and 8 Gy irradiated group, respectively. Effect of the low-dose gamma radiation on the early growth was enhanced depending on their germination enhancement. Especially, it was the highest at 2 Gy irradiated group of 70$\sim$80% germinative seed of pumpkin. Catalase activity of bottle gourd was increased at 4 Gy irradiated group. Catalase and peroxidase activity of pumpkin were increased at 2 Gy and 8 Gy irradiated group. These results suggest that the germination, early growth and physiological activity of bottle gourd and pumpkin seeds could be promoted by the low dose gamma radiation.

Detection of Cucumber green mottle mosaic virus in Bottle Gourd Seeds by RT-PCR (RT-PCR에 의한 박 종자의 오이녹반모자이크바이러스 검정)

  • Lee, Sook-Kyung;Song, Wan-Yeob;Kim, Hyung-Moo
    • Research in Plant Disease
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    • v.10 no.1
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    • pp.53-57
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    • 2004
  • Cucumber green mottle mosaic virus (CGMMV) was a major pathogen of watermelon and had affected seriously to watermelon production in Korea. Rapid and sensitive detection method of CGMMV associated with bottle gourd (Lagenafia siceraria) seeds was developed by using RT-PCR in this study. A pair of primeri Wmfl and Wmrl, specific for CGMMV was designed from coat protein gene sequences of CGMMV-W and used for amplifying 420 bp product in RT-PCR. To simplify the virus extraction procedure and reduce an inhibitor from the extract for the RT-PCR, some methods using ethanol precipitation, double filtration, polyethylene glycol precipitation and phenol/chloroform/isoamyl alcohol extraction procedure were compared and the phenol/chloroform/isoamyl alcohol extraction procedure was selected by its enhanced sensitivity. This detection method using the selected extraction step and the primers for RT-PCR could reliably detect an infected level of one CGMMV-infested seed in 1,000 seeds. This rapid and sensitive RT-PCR assay provides auseful tool for the specific detection of CGMMV in bottle gourd seed samples containing high levels of back-ground inhibitors.

First Report of Macrophomina phaseolina Causing Charcoal Rot in Bottle Gourd in Korea

  • Kim, Sang Gyu;Kim, Tae Bok;Lee, Oak Jin
    • The Korean Journal of Mycology
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    • v.49 no.3
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    • pp.399-403
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    • 2021
  • Stem blight symptoms were observed in greenhouse-grown bottle gourds (Lagenaria siceraria) in Wanju, Korea. Infected plants showed leaf chlorosis and wilting; the plants eventually died with the infected leaves remaining attached, and black sclerotia formed on the diseased stem. Based on the morphological characteristics and pathogenicity results and sequence analyses of the internal transcribed spacer, translation elongation factor 1-α, β-tubulin, and calmodulin regions, the isolated fungus was Macrophomina phaseolina. This is the first report of charcoal rot in Lagenaria siceraria caused by Macrophomina phaseolina in Korea.

Effects of Low Dose Gamma Radiation and Seed Moisture Content on Germination and Early Growth of Vegetable Crops (저선량 방사선 조사 시 종자수분함량이 채소류의 발아와 초기생장에 미치는 영향)

  • Baek, Myung-Hwa;Lee, Young-Keun;Lee, Young-Bok;Yang, Seung-Gyun;Kim, Jae-Sung
    • Korean Journal of Environmental Agriculture
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    • v.22 no.3
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    • pp.215-219
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    • 2003
  • To investigate the effects of low dose gamma radiation and seed moisture content (SMC) on germination and early growth of vegetable crops, seeds of chinese cabbage (Brassica campestris L.), radish (Raphanus sativus L.), red pepper (Capcicum annuum L.), figleaf gourd (Cucurbita ficifolia Bouche) and bottle gourd (Lagenaria siceraria Standl), with different SMC were irradiated with different doses ($0{\sim}20\;Gy$) of gamma-ray by irradiator ($^{60}Co$, ca.150 TBq of capacity, AECL). Vegetable crops in which low dose gamma radiation was irradiated in seeds with different moisture content showed different response in seed germination and early growth to low dose gamma radiation. The germination rate of chinese cabbage, figleaf ground and bottle gourd irradiated with $2{\sim}8\;Gy$ showed interactive responses against relative SMC. Also, significant interactions occurred for the early growth between those factors. The stimulating effects of gamma radiation were more pronounced for hydrated seeds of chinese cabbage, radish, figleaf gourd and bottle gourd showing prominent responses with $2{\sim}10\;Gy$ irradiation, particularly for chinese cabbage and bottle gourd. These results suggest that radiation may promote germination and early growth of vegetable crops through interaction with SMC.

Production Techniques for the Metal Artifacts Excavated in Hasong-ri, Yeongwol and Their Conservation Treatment (영월 하송리 출토 금속유물 보존처리 및 제작기법 연구)

  • Lee, Byeong Hoon;Go, Hyeong Sun;Kim, Soo-Ki
    • Conservation Science in Museum
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    • v.21
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    • pp.53-66
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    • 2019
  • This study describes the processes undertaken for the conservation treatment of metal artifacts excavated in Hasong-ri, Yeongwol, Gangwon-do and the results of the related surface composition analysis. X-ray fluorescence analysis (XRF) was applied to analyze the surface composition of a small gilt-bronze wind chime, two clappers, and a small gourd-shaped bronze bottle. The gourd-shaped bronze bottle was investigated using radiography to examine its internal structure. The wind chime and clappers were excavated from the same location. A gilt layer was identified on the wind chime, but surface corrosion made it difficult to identify any such layers on the clappers. The element analysis revealed that the wind chime was made of bronze in a Cu-Sn-Pb ternary system and was gilt-plated using mercury amalgam. The clappers were made from copper with a small amount of gold detected, but did not show any evidence of mercury. Since a thick corrosion layer was affixed to the surface of each clapper, it was impossible to identify the surface composition and determine if the clappers had been gilded. It is possible that the gold detected from the clappers was a foreign substance or had detached from the giltbronze wind chime buried alongside them. The small gourd-shaped bronze bottle was investigated through surface element analysis and radiography to verify if it was a type of silver bottle used as currency during the Goryeo dynasty. The radiography photography identified that a small hole in the middle of the base had been stopped up. The general surface analysis did not detect silver, which suggests that the bottle was made of bronze instead and was not one of the silver bottles used as a means of currency.