• The Plant Pathology Journal
• /
• 제17권6호
• /
• pp.367.2-367
• /
• 2001

• 식물병연구
• /
• 제19권1호
• /
• pp.7-11
• /
• 2013

국내 나리 주산단지에서 분리한 Botrytis elliptica 48개 균주와 Botrytis cinerea 23개 균주를 대상으로 농가포장에서 사용되고 있는 살균제에 대한 약제저항성 검정을 실시하였다. 살균제 benomyl과 mancozeb에 대한 $EC_{50}$ 값은 $500-1,000{\mu}g/ml$로 시험에 사용된 모든 약제 중에서 가장 높게 나타나 이 약제들은 나리재배 포장에서 잎마름병에 대한 방제 효과가 거의 없을 것으로 판단되었다. 또한 나리 재배농가에서 잎마름병 방제약제로 가장 많이 사용되고 있는 dicarboximide계 procymidone 살균제와 iprodione 살균제의 경우, $EC_{50}$ 값 $5-50{\mu}g/ml$은 각각 93.7%와 100%이었고, diethofencarb/carbendazim 합제와 fludioxonil 살균제의 $EC_{50}$ 값 $0-0.1{\mu}g/ml$ 범위는 각각 98.0%와 93.8%이었다. 한편, 비가림 포장 재배에서의 잎마름병 방제 효과는 발병 직후 iprodione 약제, diethofencarb/carbendazim 합제, fludioxonil 약제를 교호적으로 4회 살포하였을 때 가장 우수하였다.

• 한국균학회지
• /
• 제27권4호통권91호
• /
• pp.280-282
• /
• 1999

1999년 5월 경남 남해군 비닐하우스 내에 재배중인 네트멜론(품종: VIP)의 성숙기 과일에서 잿빛곰팡이병이 발견되었다. 병정은 멜론 과일의 꽃자리 부분 또는 옆면이 부패되고 그 위에 잿빛곰팡이가 아주 많이 형성되고 과육이 부패되었다. 병원균의 분생포자의 크기는 $8.8{\sim}21.2{\times}6.5{\sim}13.1\;{\mu}m$이고 타원형 또는 도란형이고 무색내지 담황색이다. 분생자병의 폭은 $4.3{\sim}11.4\;{\mu}m$이고 무색이고 윗부분에 분생포자가 아주 많이 형성되었다. 병반에서 분리한 병원균은 Botrytis cenerea로 동정되었고, 분생포자 현탁액을 과일에 인공접종한 결과 포장에서 발병한 과일에서의 병징과 동일한 병징이 형성되었다. 균사생육적온은 $15^{\circ}C$에서 $25^{\circ}C$였다. 조사한 포장에서 발병과율은 2.2%정도였다. 재배기간 중 시설내의 비교적 낮은 온도와 높은 습도는 잿빛곰팡이병의 발생을 야기시킨 중요한 요인이 된 것으로 추정되며 이 병을 국내에서 처음 보고한다.

• 식물병연구
• /
• 제9권3호
• /
• pp.170-173
• /
• 2003

포장에서 참다래 저장병균들에 의한 감염을 최소화할 수 있는 화학적 방제체계를 확립하기 위하여, 국내에서 참다래 저장병 약제로 등록된 베노밀 수화제와 지오판 수화제 등 2가지 약제를 대체할 수 있는 새로운 약제를 선발하기 위하여 본 연구를 실시하였다. 시험에 사용한 8가지 약제 중에서 후루실라졸 수화제, 이프로 수화제 및 터부코나졸 수화제는 모두 실내 시험을 통하여 국내에서 참다래 주요 저장병원균으로 밝혀진 Botryosphaeria dothidea, Diaporthe actinideae 및 Botrytis cinerea에 대해 탁월한 균사생장 저지효과를 나타내었을 뿐만 아니라 포장 시험에서도 베노밀 수화제와 지오판 수화제와 비등하거나 우수한 방제 효과를 나타내었으므로 장차 참다래 저장병 장제약제로 사용할 수 있을 것으로 기대된다.

• The Plant Pathology Journal
• /
• 제25권2호
• /
• pp.193-198
• /
• 2009

A full-length cDNA of OgGRP gene encoding a glycinerich cell wall protein was isolated from wild rice (Oryza grandiglumis). Deduced amino acid sequences of OgGRP are composed of 148 amino acids (16.3 kDa), and show 85.9% homology with Osgrp-2 (Oryza sativa). RT-PCR analysis showed that RNA expression of OgGRP was regulated by defense-related signaling chemicals, such as cantharidin, endothall, jasmonic acid, wounding, or yeast extract treatment. In relation to pathogen stress, the function of OgGRP was analyzed in OgGRP over-expressing Arabidopsis thaliana. Overexpression of OgGRP in Arabidopsis contributed to moderate resistance against fungal pathogen, Botrytis cinerea, by lowering disease rate and necrosis size. In the analysis of the transgenic Arabidopsis lines to check the change of gene expression profile, induction of PR1, PR5 and PDF1.2 was confirmed. The induction seemed to be caused by the interaction of ectopic expression of OgGRP with SA-and JA-dependent signaling pathways.

• 생물환경조절학회지
• /
• 제21권2호
• /
• pp.134-139
• /
• 2012

친환경 유기농자재를 이용하여 잿빛곰팡이병균의 포자 발아, 균사생장 억제효과를 기내에서 조사하였다. 그 결과 잿빛곰팡이병의 균사생장 억제 효과는 Bacillus subtilis를 주성분으로 하는 제제가 100%의 억제효과를 보였으며, 그 외의 제제는 20% 이하의 범위에서 억제효과를 나타내었다. 포자발아 억제효과는 유기황 수화제 2종('BTB', '황스타')이 각각 97.7%, 92.3%으로 나타났다. 균사생장 억제와 포자 발아억제에 모두 효과를 보이는 제제는 없었다. 따라서 잿빛곰팡이병 방제를 위해서는 포자발아 억제를 위한 방제와 균사생장억제를 위한 제제를 각각 처리하여야 할 것으로 사료되었다. 아울러 사물 기생성을 가진 잿빛곰팡이병의 방제를 위해서는 잔재물에서 생장을 막는 것이 중요하며 이를 위해 예방을 전제로 한 방제의 경우 유기황을 주성분으로 하는 제제가 적용 가능할 것으로 생각된다.

• The Plant Pathology Journal
• /
• 제17권3호
• /
• pp.149-153
• /
• 2001

A total of 2109 isolates of Botrytis cinerea were collected from infected plants fo strawberry, tomato, and cucumber in Korea from 1994 to 1996. Based on in virtotests for mycelial growth on potato-dextrose agar containing fungicides, the esolates were classified into six phenotypic groups : SSR, SRR, RSS, RRS, RSR, and RRR, representing sensitivity (S) or resistance (R) to carbendazim, procymidone, and diethofencarb. In that order the isolation frequencies of the SSR, SRR, RSS, RRS, RSR, and RRR phenotypes were 28.7, 1.1, 28.8, 39.4, 1.0, and 0.9%, respectively. Three isolates from each SSR, SRR, RSS, RRS, and RSR and an isolate of RRR phenotype were selected and evaluated for their fitness-related characteristics such as pathogenic aggressiveness, mycelial growth rate, sporulation, and sclerotial formation. Competitive abilities of the SSR, SRR, RSS, RRS, and RSR phenotypes were also compared by inculating mixtures of conidial suspensions of two phenotypes to cucumber plant, and then determining re-isolation frequencies from lesions. In general, significant differences in fitness-related characteristics, except pathogenic aggressiveness, were found not only between but also within phenotype groups. In the competitiveness tests, carbendazim-sinsitive phenotypes (SSR and SRR) were found to be more competitive than the resistant ones (RSS and RSR), whereas, the procymidone-resistant phenotypes (SRR and RRS) appeared to be more competitive than the sensitive ones (SSR, RSS, and RSR). There was no consistent dominance in competitiveness between the diethofencarb-resistant and sensitive phenotypes. The RSR phenotype was the least competitive among the five phenotypes.

• The Plant Pathology Journal
• /
• 제36권6호
• /
• pp.579-590
• /
• 2020

Botrytis cinerea, which causes gray mold disease in more than 200 plant species, is an economically important pathogen that is mainly controlled by synthetic fungicides. Synergistic fungicide mixtures can help reduce fungicide residues in the environment and mitigate the development of fungicide-resistant strains. In this study, we screened microbial culture extracts on Botrytis cinerea to identify an antifungal synergist for tebuconazole. Among the 4,006 microbial extracts screened in this study, the culture extract from Schizophyllum commune displayed the most enhanced activity with a sub-lethal dosage of tebuconazole, and the active ingredient was identified as schizostatin. In combination with 5 ㎍/ml tebuconazole, schizostatin (1 ㎍/ml) showed disease control efficacy against gray mold on tomato leaf similar to that achieved with 20 ㎍/ml tebuconazole treatment alone. Interestingly, schizostatin showed demethylation inhibitor (DMI)-specific synergistic interactions in the crossed-paper strip assay using commercial fungicides. In a checkerboard assay with schizostatin and DMIs, the fractional inhibitory concentration values were 0.0938-0.375. To assess the molecular mechanisms underlying this synergism, the transcription levels of the ergosterol biosynthetic genes were observed in response to DMIs, schizostatin, and their mixtures. Treatment with DMIs increased the erg11 (the target gene of DMI fungicides) expression level 15.4-56.6-fold. However, treatment with a mixture of schizostatin and DMIs evidently reverted erg11 transcription levels to the pre-DMI treatment levels. These results show the potential of schizostatin as a natural antifungal synergist that can reduce the dose of DMIs applied in the field without compromising the disease control efficacy of the fungicides.

• The Plant Pathology Journal
• /
• 제35권1호
• /
• pp.71-76
• /
• 2019

$SdhB^{P225F}$ and $SdhB^{H272R}$ mutations have been found associated with boscalid resistance in Botrytis cinerea from strawberry in Shanghai, China. For rapid detection of two mutations, tetra-primers were designed and optimized to gain the relatively high accuracy and specificity based on the ARMS-PCR technique, by which resistance can be identified with different lengths of products on agarose gels. The tetra-primer ARMS-PCR systems for $SdhB^{P225F}$ and $SdhB^{H272R}$ were validated by 9 SdhB-squenced strains repeatedly. Then, sensitivity of 30 more strains were also tested by the methods, which were accordant with genotypes by sequencing and the sensitivity of conidial germination to boscalid by 100%. Thus, the methods developed in this study are proved to be rapid, inexpensive, accurate and practical for resistance detection of Botrytis cinerea caused by $SdhB^{P225F}$ and $SdhB^{H272R}$ mutations.

• 생명과학회지
• /
• 제14권1호
• /
• pp.193-197
• /
• 2004

A continuous enrichment culture procedure was used to isolate bacteria from various soil sources capable of suppressing large patch disease of turfgrass. Six isolates consistently suppressed large patch in turfgrass, and ranged in the spectrum of extracellular enzymes that they expressed. The best disease- suppressing isolate, TBM912, expressed protease, CMCase, and pectinase activity and inhibited the growth of Rhizectonin solani and Betrytis cinerea in vitro. Here we show that this strain also produces an antibiotic that was identified by TLC, SDS-PACE and HPLC analysis as lipopeptide.