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Changes in Sensitivity Levels of Botrytis spp. Population Isolated from Lily to Fungicides and Control under Field Condition

나리에서 분리한 잎마름병균의 살균제에 대한 감수성 변화와 포장 방제

  • Hahm, Soosang (Agricultural Environment Research Division, Chungnam Agricultural Research and Extension Services) ;
  • Kyeong, Kicheon (Taean Lily Experiment Station, Chungnam Agricultural Research and Extension Services) ;
  • Kim, Byungryun (Agricultural Environment Research Division, Chungnam Agricultural Research and Extension Services) ;
  • Han, Kwangseop (Agricultural Environment Research Division, Chungnam Agricultural Research and Extension Services) ;
  • Choi, Jongjin (Taean Lily Experiment Station, Chungnam Agricultural Research and Extension Services) ;
  • Nam, Yunkyu (Agricultural Environment Research Division, Chungnam Agricultural Research and Extension Services) ;
  • Yu, Seunghun (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
  • 함수상 (충남농업기술원 농업환경연구과) ;
  • 경기천 (충남농업기술원 태안백합시험장) ;
  • 김병련 (충남농업기술원 농업환경연구과) ;
  • 한광섭 (충남농업기술원 농업환경연구과) ;
  • 최종진 (충남농업기술원 태안백합시험장) ;
  • 남윤규 (충남농업기술원 농업환경연구과) ;
  • 유승헌 (충남대학교 응용생물학과)
  • Received : 2012.12.07
  • Accepted : 2013.02.28
  • Published : 2013.03.31

Abstract

Forty eight isolates of Botrytis elliptica and 23 isolates of B. cinerea from several locations in Korea were tested for resistance to fungicides used in the farmer's fields. Isolation frequency of B. elliptica having $EC_{50}$ (effective concentration of 50%) value $500-1000{\mu}g/ml$ to benomyl and mancozeb appeared highly, suggesting that the two fungicides are not effective in controlling leaf blight of lily in the field. The isolates were tested for resistance to fungicides procymidone and iprodione which were most commonly used in the farmer's fields. The rates of $EC_{50}$ value $5-50{\mu}g/ml$ to procymidome and iprodione were 93.7% and 100%, respectively, and those of $0-0.1{\mu}g/ml$ to diethofencarb+carbendazim and fludioxonil were 98.0% and 93.8%, respectively. In the rain-protected cultivation, control of leaf blight of lily was the most effective when iprodine, diethofencarb+carbendazim, and fludioxonil were sprayed alternately four times during the growing season.

국내 나리 주산단지에서 분리한 Botrytis elliptica 48개 균주와 Botrytis cinerea 23개 균주를 대상으로 농가포장에서 사용되고 있는 살균제에 대한 약제저항성 검정을 실시하였다. 살균제 benomyl과 mancozeb에 대한 $EC_{50}$ 값은 $500-1,000{\mu}g/ml$로 시험에 사용된 모든 약제 중에서 가장 높게 나타나 이 약제들은 나리재배 포장에서 잎마름병에 대한 방제 효과가 거의 없을 것으로 판단되었다. 또한 나리 재배농가에서 잎마름병 방제약제로 가장 많이 사용되고 있는 dicarboximide계 procymidone 살균제와 iprodione 살균제의 경우, $EC_{50}$$5-50{\mu}g/ml$은 각각 93.7%와 100%이었고, diethofencarb/carbendazim 합제와 fludioxonil 살균제의 $EC_{50}$$0-0.1{\mu}g/ml$ 범위는 각각 98.0%와 93.8%이었다. 한편, 비가림 포장 재배에서의 잎마름병 방제 효과는 발병 직후 iprodione 약제, diethofencarb/carbendazim 합제, fludioxonil 약제를 교호적으로 4회 살포하였을 때 가장 우수하였다.

Keywords

References

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