• Korean Journal of Plant Resources
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• v.19 no.1
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• pp.68-75
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• 2006

This study was conducted to investigate the combination of proper culture medium for differentiation culture of Chrysanthemum zawadskii var. latilobum KITAMURA. The combinations of culture medium were consisted with an inorganic materials such as vermiculite, perlite, saprolite, sand and upland top soil, and with an organic materials such as peat-moss, leaf mold and compost. For the plant growth characteristics, the plant height, number of tiller, root length and root weight in the combination of leaf mold was relatively great as compared to the treatment with peat-moss and compost. It was considered that humus might be contributed to improve the physical properties of soil as well as the sequential nutrient supply. For the combination of peat-moss, the plant growth was not good because of not sufficient nutrient supply, Also, the plant growth in the treatment of compost was so bad because of enhancement of culture medium pH 8.9 and increasement of phosphorous content.

• Proceedings of the Botanical Society of Korea Conference
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• 1987.07a
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• pp.191-211
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• 1987

Common usage of the concept of juvenility implies that there is one physiological phase, the juvenile phase, which manifests itself in the various morphological and physiological phenomena observed in juvenile higher plants. The juvenile phase is often defined as that time from seed germination until the plant attains the ability to flower regulating such behaviour. This definition precludes plants from flowering in the juvenile phase. It is of major interest, therefore, to identify the physiological controls(Bluehreife) regulating such behavior. The length of the juvenile period in higher plants ranges from one year to over 60 years in different species. The long juvenile period of seedling is the main cause of the long duration of the breeding process. I determined the length of the juvenile period in various plants and its control of phase changes in natural system in relation to factors such as plant size and age, shoot morphology, apex size, root system and phytohormonal and nutritional status is reviewed. From the own experimental and observational evidence available it appears that both hormonal and nutritional factors can be involved in control of juvenility but that a specific juvenile or flowering hormone is not involved. Grafting, ringing, scoring, root pruning and fertilization have been used to accelerate flowering, but in most cases these cultured treatments are only successful on plants that were passed the juvenile phase. It is suggested that there are intrinsic difference between the meristematic cells of the apieces of juvenile and adult shoot, which are thus determined with respect to there development potentialities. The problems associated with the maintenance of the determined state through mitosis are discussed. The properties of transitional forms of Ribes nigrum L. intermediate between the juvenile and adult phase, are descrived and there implications discussed. Analogies are drawn between juvenile phenomena in woody perennials and in herbaceous species.

• Food Science of Animal Resources
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• v.39 no.5
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• pp.844-861
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• 2019

Over the last few years, marine environment was found to be a source of surplus natural products and microorganisms with new bioactive secondary metabolites of interest which can divulge nutritional and biological impact on the host. This study aims to assess the possible, inherent and functional probiotic properties of a novel probiotic strain Enterococcus durans F3 (E. durans F3) isolated from the gut of fresh water fish Catla catla. Parameters for evaluating and describing the probiotics described in FAD/WHO guidelines were followed. E. durans F3 demonstrated affirmative results including simulated bile, acid and gastric juice tolerance with exhibited significant bactericidal effect against pathogens Staphylococcus aureus, Salmonella Typhi, Escherichia coli and Pseudomonas aeruginosa. This can be due to the enterocin produced by E. durans F3 strain, which was resolute by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) gel with amplification of the anticipated fragment of a structural gene; enterocin A, followed by antibiotic susceptibility assessment. Effective antioxidant potentiality against ${\alpha}$-diphenyl-${\alpha}$-picrylhydrazyl free radicals including lipase, bile salt hydrolase activity with auto-aggregation and cell surface hydrophobicity was similarly observed. Results are proving the potentiality of E. durans F3, which can also be used as probiotic starter culture in dairy industries for manufacturing new products that imparts health benefits to the host. Finding the potent and novel probiotic strains will also satisfy the current developing market demand for probiotics.

• Journal of The Korean Society of Grassland and Forage Science
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• v.29 no.3
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• pp.227-234
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• 2009

This study was carried out to determine the effect of establishment of organic pasture by hoof cultivation with Korean black goat on productivity of grassland and properties of soil. This study was conducted from August, 2005 to September, 2008 at Cheonan, Korea. Experiment was designed composing with 4 treatment : Standard (Control, T1), Grazing after application of cattle manure (T2), Application of cattle manure after grazing (T3), Application of cattle manure at half of grazing (T4). The establishment ratio of pasture in control treatment increased significantly as compared with that of organic treatment(p<0.05). The yield of dry matter (DM) of pasture in control treatment increased significantly as compared with that of organic treatment. The contents of crude protein (CP), acid detergent fiber (ADF), neutral detergent fiber (NDF) and dry matter digestibility (DMD) of pasture were not influenced by cattle manure and grazing method. Grasses coverage ratio in grassland in second year after grassland development were higher than that of first year. The contents of organic matter (OM) and $P_2O_5$ in soil samples collected in organic treatment at the end of the experiment were higher than those of control. However, The pH, and the concentrations of CEC (Ca, Na and Mg) in soil samples collected in organic treatment were hardly influenced, as compared with those at the beginning of the experiment. This study suggests that the grasses and legumes coverage ratio in grassland can be improved by suitable pasture management.

• Journal of Microbiology and Biotechnology
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• v.18 no.8
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• pp.1386-1392
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• 2008

A gene (sll0158) putatively encoding a glycogen branching enzyme (GBE, E.C. 2.4.1.18) was cloned from Synechocystis sp. PCC6803, and the recombinant protein expressed and characterized. The PCR-amplified putative GBE gene was ligated into a pET-21a plasmid vector harboring a T7 promoter, and the recombinant DNA transformed into a host cell, E. coli BL21(DE3). The IPTG-induced enzymes were then extracted and purified using Ni-NTA affinity chromatography. The putative GBE gene was found to be composed of 2,310 nucleotides and encoded 770 amino acids, corresponding to approx. 90.7 kDa, as confirmed by SDS-PAGE and MALDI-TOF-MS analyses. The optimal conditions for GBE activity were investigated by measuring the absorbance change in iodine affinity, and shown to be pH 8.0 and $30^{\circ}C$ in a 50 mM glycine-NaOH buffer. The action pattern of the GBE on amylose, an $\alpha$-(1,4)-linked linear glucan, was analyzed using high-performance anion-exchange chromatography (HPAEC) after isoamylolysis. As a result, the GBE displayed $\alpha$-glucosyl transferring activity by cleaving the $\alpha$-(1,4)-linkages and transferring the cleaved maltoglycosyl moiety to form new $\alpha$-(1,6)-branch linkages. A time-course study of the GBE reaction was carried out with biosynthetic amylose (BSAM; $M_p{\cong}$8,000), and the changes in the branch-chain length distribution were evaluated. When increasing the reaction time up to 48 h, the weight- and number-average DP ($DP_w$ and $DP_n$) decreased from 19.6 to 8.7 and from 17.6 to 7.8, respectively. The molecular size ($M_p$, peak $M_w{\cong}2.45-2.75{\times}10^5$) of the GBE-reacted product from BSAM reached the size of amylose (AM) in botanical starch, yet the product was highly soluble and stable in water, unlike AM molecules. Thus, GBE-generated products can provide new food and non-food applications, owing to their unique physical properties.

• Korean Journal of Plant Resources
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• v.20 no.4
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• pp.342-347
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• 2007

The present study was conducted to examine the effect of prunus mume extract on the Kimchi fermentation. The physicochemical and microbiological properties of Kimchi with the addition of various levels(0, 0.1, 0.3 or 0.5%)of prunus mume extract was measured up to 30 days at $4^{\circ}C$. pH was decreased sharply in control after 2 days . A typical decreasing pattern in pH values was noted in all treatments during the initial 18 days of fermentation followed by a gradual decrease afterwards. Total acidity was decreased by the addition of prunus mume extract. Reducing sugar content was reduced to approximately 70% by day 10-12 in prunus mume extract treatments. Total vitamin C content was reached to maximum on 6 days of fermentation and then decreased in all treatments. Reducing sugar and total vitamin C contents were slightly increased by the addition of prunus mume. Total viable cell number increased to reach peak on day 8 and then slowly decreased during the fermentation. Lactobacillus cell number in control treatment continued to increase to reach peak on day 12, while the numbers in prunus mume treatments reached peak on day 8-12 and then gradually decreased throughout the fermentation. The results of this study indicate that prunus mume extract causes to delay the Kimchi fermentation by slowing down pH drop and inhibiting the Lactobacillus cell growth.

• Journal of Korean Society of Forest Science
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• v.85 no.2
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• pp.210-224
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• 1996

The SLODSVAT consists of interrelated submodels that simulate : the transfer of radiation, water vapour, sensible heat, carbon dioxide and momentum in two canopy layers determined by environmental conditions and ecophysiological properties of the vegetation ; uptake and storage of water in the "root-stem-leaf" system of plants ; interception of rainfall by the canopy layers and infiltration and storage of rain water in the four soil layers. A comparison of the results of modeling experiments and field micro-climatic observations in a spruce forest(Picea abies [L].Karst) in the Soiling hills(Germany) shows, that the SLODSVAT can describe and simulate the short-term(diurnal) as well as the long-term(seasonal) variability of water vapour and sensible heat fluxes adequately to natural processes under different environmental conditions. It proves that it is possible to estimate and predict the transpiration and evapotranspiration rates for spruce forest ecosystems on the patch and landscape scales for one vegetation period, if certain meteorological, botanical and hydrological information for the structure of the atmospheric boundary layer, the canopy and the soil are available.

• Journal of The Korean Society of Grassland and Forage Science
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• v.9 no.1
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• pp.34-42
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• 1989

This pot experiments were conducted to find out the optimal fertilization ratios of ${\Sigma}anion$ : ${\Sigma}cation$, ${\Sigma}A$/${\Sigma}C$, and the optimal application rates of total major nutrients in an orchardgrass/ladino clover mixed sward. The optimum ratios and concentrations in equivalent basis were computed by the Homes systematic variations technique. The results were summarized as follows; 1. The optimum fertilization ratios of ${\Sigma}A$ : ${\Sigma}C$ and the optimum application rates of total nutrients for the high yields by forage species in a mixed sward were obtained (Table 6 in detail); ${\Sigma}A$ : ${\Sigma}C$ = 2 : 1 at 80 and 320 meq/pot, and 3 : 2 at 560 and 800 meq for grass and grass plus legume, and ${\Sigma}A$ : ${\Sigma}C$ = 1 : 2 for legume in general. 2. The optimum application rates of total nutrients for the high yields of grass and grass plus kgum were increased by decreasing the ${\Sigma}A$/ ${\Sigma}C$: ratio, whereas these for legume showed a valible. range without significance. 3. The yields 01 grass and grass plus legume were generally increasing by increasing both the ${\Sigma}A$/ ${\Sigma}C$ ratio and total concentration, but they were significantly higher at the ${\Sigma}A$/ ${\Sigma}C$ = 1.273 than at the 2.125 under the high total ion concentration. The legume yields were proportional to ${\Sigma}C$ ratio and increased by increasing the total ion concentration under the condition of high ${\Sigma}C$ ratio. 4. The efficiencies of ${\Sigma}A$ and ${\Sigma}C$ in relation to the grass and grass plus legume yields were highest with the low ratios of each other and the low rates of total nutrients ${\Sigma}A$ efficiency m the legume yield tended to be similar to that of ${\Sigma}A$ in the grass yield noted above. The ${\Sigma}C$ efficiency in the legume yield, however, was generally proportional to the ${\Sigma}C$ ratio except at the low rate of 80 meqlpot. 5. The yields of grass plus legume, yield components and botanical compositions in a mixed sward were greatly influenced by the ${\Sigma}A$/${\Sigma}C$ ratios, the fertilization rates of total nutrients, and the interaction of ratio and rate noted above. These effects were generally different and opposite accading to grass and legume. In addition, the soil chemical properties and mineral contents of forages were partially influenced by these systematic variations.

• Journal of the Korean Institute of Landscape Architecture
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• v.45 no.6
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• pp.28-39
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• 2017

The purpose of this study was to analyze the tendencies of the written test questions regarding the scientific plant names in the subject of scenic planting in the national qualification test for landscape engineers. To achieve this purpose, targeting the 20 questions in the subject of scenic planting, the study chronologically analyzed the types of the presented questions about the scientific names of the plants, the changes in their year-to-year number, and their presentation frequency over the past 11 years from 2006 to 2016. The following was found by an analysis of the tendency of the questions presented over 11 years from 2006 to 2016 regarding the scientific plant names in scenic planting in the written test of the national technical qualification examination for landscape engineers. The number of these questions was 234, and they were classified into 13 items: scientific names, families/genuses/ species, characteristics, barks, roots, foliage, flowers, fruits, properties, places of origin, stalks, leave, and uses. After analyzing the examination question trends per year, the year in which the highest number of questions was given was 2013 at 56.6%; the ratio of questions asking botanical names increased until 2013, although that ratio decreased starting in 2014 due to consistent efforts from the landscaping field. The plant species most frequently asked about were Betula platyphylla and Taxus cuspidata and others frequently asked about included Pinus densiflora, Acer palmatum, Forsythia koreana, Cercis chinensis, Robinia pseudoacacia, Cornus officinalis, Zelkova serrata, Abies holophylla, Camellia japonica, Sophora japonica, Chaenomeles sinensis, which were asked about more than 10 times. The number of plant species presented as question items or choice items was 240 and their presentation frequency was 806, Among these, 66 species were steadily presented more than five times. They were presented 486 times, accounting for 27.5% of all species. The number of the new plant species which began to be presented in 2009 were 119(49.58% including Quercus myrsinaefolia) of all 240 species, indicating that their presentation frequency was gradually increased. As a result of comparative analysis of questions on arbor and shrub, evergreen and leaf abscission that have been presented for recent 11 years, the questions on arbor were more than shrub, the questions on leaf abscission were more than evergreen.

• Korean Journal of Soil Science and Fertilizer
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• v.19 no.3
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• pp.231-238
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• 1986

This field experiment was undertaken to assess the effects of three levels of inclination ($10^{\circ}$, $20^{\circ}$ and $30^{\circ}$) and four rates of $N-P_2O_5-K_2O$ (0-0-0, 14-10-10, 28-25-25, and 42-40-40 kg/10a) on the hilly pasture concerning to establishment, forage yield and quality, and botanical composition in the mixed grass-clover sward set up on a new reclaimed woodland by an oversowing method. This part was concerned with the concentration and mutual balance of mineral nutrients in relation to grass tetany hazard. The results obtained were summarized as follows: 1. Concentration of exchangeable Mg, relative proportions of Mg to CEC, K, and Ca in soil were considerably below the critical level for good forage growth and prevention of grass tetany. These properties were rather worsened by increasing the inclination grade and the NPK rate. 2. Compared with the critical level for the likelihood of tetany (Mg < 0.2%, K > 2.5%, and K/(Ca+Mg) equivalent ratio > 2.2 in forages in DM basis), mean concentration of Mg in mixed forages turned out to range from 0.09 to 0.14%. 2.6% of K concentration and 2.5 of K/(Ca+Mg) equivalent ratio were shown to be at a normal NPK rate. The likelihood of grass tetany was generally enhanced by increasing the inclination grade and the NPK rate. 3. The increasing of the inclination grade and NPK rate rather aggravated the low Mg concentration and unbalanced mutual ratios of mineral nutrients, from which resulted the grass tetany hazard and the depression of forage growth. To prevent these two resulted factors, Mg fertilization on grassland and/or extra Mg supplement to dairy cattle needed to be carried out. 4. The Ca/P ratios in the mixed forages ranging from 1.0 to 2.0 were reduced by the increased rate of NPK, whereas the difference due to inclination grade was yet to be recognized.