• ALGAE
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• v.29 no.4
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• pp.311-319
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• 2014

The biocide sodium hypochlorite (NaOCl) is widely used for controlling algal growth, and this application can be extended to marine environments as well. This study evaluates the biocidal efficiency and cellular toxicity of NaOCl on the harmful dinoflagellate Cochlodinium polykrikoides, with emphasis on pigment production and antioxidant enzyme activity. The test organism showed dose-dependent decrease in growth rate on exposure to NaOCl, and the 72 h $EC_{50}$ was measured to be $0.584mg\;L^{-1}$. NaOCl significantly decreased pigment levels and chlorophyll autofluorescence intensity, indicating possible detrimental effects on the photosystem of C. polykrikoides. Moreover, it significantly increased the activities of antioxidant enzymes, suggesting the production of reactive oxygen species in the cells. These data indicate that NaOCl exerted deleterious effects on the photosynthetic machinery and induced oxidative damage in the dinoflagellate and this biocide could be effectively used for the control of algal blooms.

• Ocean Science Journal
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• v.41 no.1
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• pp.31-41
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• 2006

Seasonal changes of abundance of the main phytoplankton groups of species (diatoms, dinoflagellates, chrysophytes, small flagellates and cryptophytes) and a set of environmental parameters were investigated in coastal and pre-estuarine waters of Peter the Great Bay (East/Japan Sea) in May-October of 1998 and 1999. Three periods of mass development were revealed: spring, summer and autumn blooms, with successive change of species. The conditions favourable for each group of species were determined. Driving mechanisms of the succession include nutrients transport through seasonal pycnocline by turbulent mixing, terrestrial nutrients supply by monsoon floods, nutrients supply by upwellings, and light control by the thickness of upper mixed layer. Summer succession could be explained by a simple SST-MLD diagram similar to Pingree S-kh diagram with sea surface temperature as indicator of stratification (S) and mixed layer depth as indicator of light availability (kh).

• Journal of Korean Society on Water Environment
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• v.33 no.1
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• pp.8-14
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• 2017

Microcystis aeruginosa (M. aeruginosa) is a cyanobacterium species that can form harmful algal blooms in freshwater bodies worldwide. The use of pine needle extract (PNE) to control nuisance algae by allelopathic inhibition will be environmentally friendly and promising. PNE removed successfully upto 98% of M. aeruginosa at the following optimal conditions: pH 7, $25^{\circ}C$ of temperature, 100 rpm of mixing rate, 5 min of mixing time. These results was indicated that the amount of 1 g/L PNE was removed 1g dryweight/L of M. aeruginosa. The kinetic data showed substrate inhibition kinetics and maximum growth rate was obtained when the M. aeruginosa was grown in medium containing 0.5 g/L of initial concentration of PNE. Different substrate inhibition models were fitted to the kinetic data and found the Luong model was best. The model predicted kinetic parameters were in agreement with the experimental findings. The natural extract, PNE, can be a promising inhibition due to its high efficiency and low dose requirements.

• Proceedings of KOSOMES biannual meeting
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• 2001.10a
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• pp.137-144
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• 2001

A Plankton ecosystem model was developed to investigate effects of hydrodynamic processes including advection and diffusion on size-structured phytoplankton dynamics in the mesohaline zone of the York River estuarine system, Virginia, USA. The model included 12 state variables representing the distribution of carbon and nutrients in the surface mixed layer. Groupings of autotrophs and heterotrophs were based on cell site and ecological hierarchy Forcing functions included incident radiation, temperature, wind stress, mean How and tide which includes advective transport and turbulent mixing. The ecosystem model was developed in FORTRAN using differential equations that were solved using the 4th order Runge-Kutta technique. The model showed that microphytoplankton blooms during winter-spring resulted from a combination of vertical advection and diffusion of phytoplankton cells rather than in-situ production in the lower York River estuary.

• Journal of Environmental Science International
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• v.8 no.1
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• pp.101-106
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• 1999

The relationship between primary productivity and changes in water quality was investigated at Mulgum station, a site downstream of the Nakdong River, Korea. Phytoplankton production was characterized by blooms of Microcystis aeruginosa during the summer and Stephanodiscus hantzschii during the winter. Primary production and secondary production by bacterioplankton ranged from 1.5~53.5 mg-C/ι day and 0.1~0.3 mg-C/ι day, respectively. Distribution of total organic carbon appeared to be highly correlated with phytoplankton biomass, especially during blooms of M. aeruginosa, when particulate organic carbon was 81% of total organic carbon and the main source of organic materials supplied into the water. The correlation coefficient between chlorophyll-a and BOD was 0.86. Thus it was concluded that autochthonous phytoplankton mostly affected the BOD level. Total bacterial numbers were also highly correlated with chlorophyll-a ($r^2$= 0.84) and the bacterial community appears to be regulated by phytoplankton biomass in this area.

• ALGAE
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• v.17 no.4
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• pp.283-292
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• 2002

• Proceedings of the Microbiological Society of Korea Conference
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• 2005.05a
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• pp.33-35
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• 2005

• Proceedings of the Korean Society of Environment and Ecology Conference
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• 2011.04a
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• pp.57-58
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• 2011

• Journal of the korean society of oceanography
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• v.34 no.2
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• pp.86-94
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• 1999

An investigation was conducted to determine limiting nutrients in the bay systems of the southern coastal area of Korea. The elemental composition of C, N, and P in suspended particulate matter was monitored nearly monthly in Chinhae and Koje Bays and seasonally in Deukryang Bay for 2 years. Atomic C:N ratio in particulate matter ranges from 4.3 to 9.6, typical of marine phytoplankton. C:P and N:P ratios vary from the Redfield ratio to 229 (C:P) and 37 (N:P). A constant C:N ratio of 6.87 from regression of particulate C and N concentrations demonstrates that the particulate matter in the systems originates from primary production. C:P and N:P ratios from regression of C on P and N on P are well associated with changes in salinity. The low N:P ratio of 13.1 implies N limitation in the environments of the systems. This seems to result from the low N:P ratio of nutrients released across sediment-water interface. Phytoplankton response, expressed here as the increase of chlorophyll a, to N addition also verifies N limitation for phytoplankton communities. In heavy rainfall season (from June to September), the addition of excessive N via streams into the stratified coastal water proliferates phytoplankton greatly. During the phytoplankton blooms, C:P and N:P ratios are much higher than the Redfield ratio, implying P limitation. This results from the high N:P ratio in nutrients supplied from stream waters. Strong stratification during the blooms also interrupts the supply of nutrients, particularly p, from bottom waters. Dependent upon precipitation, this tendency shows great inter-annual variation.

• Korean Journal of Ecology and Environment
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• v.41 no.4
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• pp.530-540
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• 2008

The temporal and spatial variations of size-structured phytoplankton dynamics in Youngsan Lake were investigated to explore potential mechanims controlling the dynamics in the Youngsan Lake. Field data were collected monthly from February to October, 2003 at 6 stations along the axis of Youngsan Lake. In this study, phytoplankton (chlorophyll $\alpha$) were categorized into three size classes: micro-size ($>20{\mu}m$), nano-size ($2{\sim}20{\mu}m$) and pico-size ($<20{\mu}m$). Water temperature, light attenuation coefficients, PAR (photosynthetically active radiation) and suspended solids were measured to analyze relationship between physical-chemical properties and size structure of phytoplankton. Phytoplankton blooms developed during March, July and October in the upper region of the main stem whereas small-scaled spring bloom was observed in the lower region. The scales of phytoplankton blooms were higher in the upper regions than the lower region and blooms were predominated by micro-size class in upper region but predominated by nano-size class in lower region. Growth of size-structured phytoplankton appeared to be controlled by rather light availability than temperature-dependant metabolisms in the system. Phytoplankton growth may be also supported by ambient nutrients available in the water column from analyses of chlorophyll $\alpha$ vs. nutrient concentrations including nitrite+nitrate and orthophosphate. Growth of nano-sized phytoplankton alone appeared to be supported by orthophosphate as well as nitrite+nitrate indicating that response of phytoplankton to nutrient inputs may be size-dependent.