• KOREAN JOURNAL OF CROP SCIENCE
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• v.38 no.6
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• pp.499-506
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• 1993

The contents of sulfur, sulfur-containing protein and amino acids of soybean seeds of 518 genotypes as well as their inheritance and selection efficiency in early breeding generation were measured to facilitate breeding for soybean with high sulfur-containing amino acids. Average seed sulfur content of 518 cultivars was 0.33%, and ranged from 0.20 to 0.45%, and that of 30 wild soybeans was 0.35%, and ranged form 0.19 to 0.62%. Correlation coefficients between seed sulfur content and sulfur-containing protein and amino acids were 0.924$^{**}$ and 0.974$^{**}$, respectively. Seed sulfur content was tended to be high in soybean genotypes with late maturity, seed coat bloom, or green cotyledon. Sulfur content had -0.312$^{**}$ correlation coeficient with sugar content and -0.384$^{**}$ with 100 seed weight. Seed sulfur content was inherited quantitatively, in which additive effect was greater than dominant one, and proportion of genes with positive effects was similar to those with negative ones. Estimated narrow- and broad-sense heritabilities were 0.75 and 0.88 for seed sulfur content, respectively. Heritability measured from selection in early breeding lines for high or low seed sulfur content was 60～62.5% or 50～62,5%, respectively. And selection for high sulfur content increased by 14.7～18.8%, whereas that for low one decreased by 8.8～15.6%, when compared to that of random population. Therefore selection in early generation seemed to be clearly effective.

• Korean Journal of Environmental Biology
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• v.41 no.2
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• pp.109-125
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• 2023

This study was conducted to select target fish species as baseline research for accumulation analysis of major hazardous chemicals entering the aquatic ecosystem in Korea and to analyze the impact on fish community. The test bed was selected from a sewage treatment plant, which could directly confirm the impact of the inflow of harmful chemicals, and the Geum River estuary where harmful chemicals introduced into the water system were concentrated. A multivariable metric model was developed to select target candidate fish species for hazardous chemical analysis. Details consisted of seven metrics: (1) commercially useful metric, (2) top-carnivorous species metric, (3) pollution fish indicator metric, (4) tolerance fish metric, (5) common abundant metric, (6) sampling availability (collectability) metric, and (7) widely distributed fish metric. Based on seven metric models for candidate fish species, eight species were selected as target candidates. The co-occurring dominant fish with target candidates was tolerant (50%), indicating that the highest abundance of tolerant species could be used as a water pollution indicator. A multi-metric fish-based model analysis for aquatic ecosystem health evaluation showed that the ecosystem health was diagnosed as "bad conditions". Physicochemical water quality variables also influenced fish feeding and tolerance guild in the testbed. Eight water quality parameters appeared high at the T1 site, indicating a large impact of discharging water from the sewage treatment plant. T2 site showed massive algal bloom, with chlorophyll concentration about 15 times higher compared to the reference site.

• Korean Journal of Ecology and Environment
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• v.56 no.1
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• pp.94-103
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• 2023

Cyanobacterial resting cells, such as akinetes, are important seed cells for cyanobacteria's early development and bloom. Due to their importance, various methods have been attempted to isolate resting cells present in the sediment. Ludox is a solution mainly used for cell separation in marine sediments, but finding an accurate method for use in freshwater is difficult. This study compared the two most commonly used Ludox methods (direct sediment treatment and sediment distilled water suspension treatment). Furthermore, we proposed a highly efficient method for isolating cyanobacterial resting cells and eDNA amplification from freshwater sediments. Most of the resting cells found in the sediment were akinete to the Nostocale and were similar to those of Dolichospermum, Cylindrospermum, and Aphanizomenon. Twenty times more akinetes were found in the conical tube column using the sediment that had no treatment than in the sample treated by suspending the sediment in distilled water. Akinete separated through Ludox were mainly spread over the upper and lower layers in the column rather than concentrated at a specific depth in the column layer. The mibC, Geo, and 16S rDNA genes were successfully amplified using the sediment directly in the sample. However, the amplification products of all genes were not found in the sample in which the sediment was suspended in distilled water. Therefore, 5 g to 10 g of sediment is used without pretreatment when isolating cyanobacterial resting cells from freshwater sediment. Cell isolation and gene amplification efficiency are high when four times the volume of Ludox is added. The Ludox treatment method presented in this study isolates cyanobacterial resting cells in freshwater sediment, and the same efficiency may not appear in other biotas. Therefore, to apply Ludox to the separation of other biotas, it is necessary to conduct a pre-experiment to determine the sediment pretreatment method and the water layer where the target organism exists.

• Korean Journal of Ecology and Environment
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• v.56 no.1
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• pp.36-44
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• 2023

Environmental DNA (eDNA) can exist in both intracellular and extracellular forms in natural ecosystems. When targeting harmful cyanobacteria, extracellular eDNA indicates the presence of traces of cyanobacteria, while intracellular eDNA indicates the potential for cyanobacteria to occur. However, identifying the "actual" potential for harmful cyanobacteria to occur is difficult using the existing sediment eDNA analysis method, which uses silica beads and cannot distinguish between these two forms of eDNA. This study analyzes the applicability of a density gradient centrifugation method (Ludox method) that can selectively analyze intracellular eDNA in sediment to overcome the limitations of conventional sediment eDNA analysis. PCR was used to amplify the extracted eDNA based on the two different methods, and the relative amount of gene amplification was compared using electrophoresis and Image J application. While the conventional bead beating method uses sediment as it is to extract eDNA, it is unknown whether the mic gene amplified from eDNA exists in the cyanobacterial cell or only outside of the cell. However, since the Ludox method concentrates the intracellular eDNA of the sediment through filtration and density gradient, only the mic gene present in the cyanobacteria cells could be amplified. Furthermore, the bead beating method can analyze up to 1 g of sediment at a time, whereas the Ludox method can analyze 5 g to 30 g at a time. This gram of sediments makes it possible to search for even a small amount of mic gene that cannot be searched by conventional bead beating method. In this study, the Ludox method secured sufficient intracellular gene concentration and clearly distinguished intracellular and extracellular eDNA, enabling more accurate and detailed potential analysis. By using the Ludox method for environmental RNA expression and next-generation sequencing (NGS) of harmful cyanobacteria in the sediment, it will be possible to analyze the potential more realistically.

• Journal of The Korean Society of Grassland and Forage Science
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• v.42 no.1
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• pp.10-16
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• 2022

This study was conducted to examine the dry matter yield and weed control of alfalfa according to postemergence herbicides treatment during spring seeding alfalfa. The seeding time of alfalfa was April 21, 2021, the seeding amount was 20 kg/ha, and the seeding method was by 20 cm wide. The alfalfa harvest was carried out at the early bloom stage (10% of flowering), and the harvest date was June 29, 2021. The test treatments were non herbicide (NH), hand weeding (HW), herbicide 1 (Trifluralin, H1), herbicide 2 (S-metolachlor, H2), herbicide 3 (Alachlor, H3), and herbicide 4 (Pendimethalin, H4). Alfalfa plant height was significantly highest in H2 (62.1±1.4 cm) followed by H3 (61.7±1.6 cm), HW (58.5±1.0 cm), H1 (57.2±1.3 cm), H4 (56.1±1.3 cm), and NH (54.1±1.2 cm) (p<0.05). Based on HW, H2 and H3 were high and H1 and H4 were short, but NH was significantly shorter than HW and H1~H4 (p<0.05). The dry matter yield of alfalfa in NH, HW, H1, H2, H3, and H4 were 717.2±94.2, 2,613.8±254.1, 1,667.8±94.1, 2,498.3±120.2, 2,435.0±118.3, and 1,793.7±354.3 kg/ha. HW is the highest among them (p<0.05). The feed composition of alfalfa was 22~24% of the dry matter yield, and the CP content were significantly higher in NH (23.6 %) (p<0.05). The NH had higher (p<0.05) NDF and ADF, but RFV was lower (p<0.05). The weed plant height was NH 98.0±3.3cm, HW 73.3±1.7 cm, H1 91.9±1.5 cm, H2 53.3±5.8 cm, H3 81.4±3.5 cm and H4 96.6±2.2 cm, and H2 was significantly smallest in the group (p<0.05). The weed dry matter yield was NH 4,770.4±232.5 kg/ha, HW 316.3±91.9 kg/ha, H1 2,353.4±173.7 kg/ha, H2 114.5±10.2 kg/ha, H3 752.7±440.6 kg/ha and H4 2,220.6±775.6 kg/ha. The weed control value was HW 94.1%, H1 53.5%, H2 98.2%, H3 84.9%, H4 48.7%, the weed value of H2 is similar to weed control value of HW. Considering the above results, postemergence herbicide treatment controlled weeds by more than 50% compared with no treatment, and among herbicides, H2 (S-metolachlor) was found to be on a similar level to hand weeding.

• Journal of Marine Life Science
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• v.2 no.2
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• pp.62-69
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• 2017

In order to assess the potential environmental factors in the vicinity of a fish cage farm, we investigated the biotic and abiotic factors during a short-term period in summer 2016 in two inner stations of Tongyeong Obi. High water temperature on August 10^th was apparent among the full depth of up to 29℃, which might have been related to the abnormally high temperatures of large amounts of the Changjiang River discharge along the Tongyeong coast. The concentration of nitrate+nitrite, ammonium, phosphate, and silicate ranged from 0.08 to 5.11 μM, 0.08 to 34.62 μM, 0.01 to 1.15 μM, and 1.46 to 31.79 μM, respectively. The nutrients were mainly supplied by precipitation and leaching from the bottom sediments in the fish culture farm area. It was not retained for a long duration because of the phytoplankton consumption and diffusion by water currents. The chlorophyll a concentration varied from 0.49 ㎍ l^-1 to 7.39 ㎍ l^-1. At that time, Chaetoceros debilis, C. pseudocurvisetus, and Pseudo-nitzschia delicatissima were rapidly proliferated and reached the level of 4.74 × 10⁹ cells l^-1. In particular, the lowest dissolved oxygen was recorded at 4.52 ㎍ l^-1 at the bottom layer after bloom. Therefore, even though phytoplankton blooms in summer have frequently occurred in a fish culture farm area, the oxygen-deficient environments were not found in neither the surface nor bottom layers, which implied that the water masses might be well exchanged from the mouth of the northwest and southeast between Obi and Mireuk Island in the study area.

• Journal of Bio-Environment Control
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• v.32 no.3
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• pp.249-255
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• 2023

Korean melon (Cucumis melo L.) is grown mostly in Northeast Asia area, and as a fruit mainly produced in Korea, the yield per unit area continues to improve, but the cultivation method is limited to soil cultivation, so it is necessary to develop hydroponic cultivation technology for scale and labor-saving is needed. As the ratio of NO₃^- increased, the plant height, the leaf length, the leaf width, and the internode length became longer and larger. On the other hand, the SPAD value decreased. The lower the ratio of NO₃^-, the faster the female flower bloom, and there was no difference in fruit maturity between treatments. There was no difference in the shape of fruit according to the ratio of NO₃^-, and the hardness was higher as the ratio of NO₃^- was lower. The total yield from March to July was KM3 5,650 kg/10a and KM1 4,439 kg/10a, 27% higher in KM3 and, in particular, 36% higher in quantity from March to May, when Korean melon prices were high season. Therefore, it was judged that it would be appropriate to supply NO₃^- suitable for hydroponic cultivation of Korean melon, which was formalized in December and produced from spring, at the level of 6.5 to 10 me·L^-1.

• Journal of Wetlands Research
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• v.25 no.4
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• pp.274-283
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• 2023

Buffer zones, an example of nature-based solutions, offer wide range of environmental, social and economic benefits due to their multifunctionality when applied to watershed areas promoting blue-green connectivity. This study evaluated the effects of buffer zone application to the water quality of Yongdam reservoir tributaries. Particularly, the challenges and improvement of the buffer zone design were identified and suggested, respectively. Water and soil samples were collected from a total of six sites in Yongdam reservoir from September 2021 to April 2022. Water quality analyses revealed that among the sites monitored, downstream of Sangjeonmyeon Galhyeonri (SG_W_D2) was found to have the highest concentration for water quality parameters turbidity, total suspended solids (TSS), chemical oxygen demand (COD), total phosphorus (TP) and total nitrogen (TN). This finding was attributed to the algal bloom observed during the sampling conducted in September and October 2021. It was found through the soil analyses that high TN and TP concentrations were also observed in all the agricultural land uses implying that nutrient accumulation in agricultural areas are high. Highest TN concentration was found in the agricultural area of Jeongcheonmyeon Wolpyeongri (JW_S_A) followed by Jucheonmyeon Sinyangri (JS_S_A) while the lowest TN concentration was found in the original soil of Sangjeonmyeon Galhyeonri (SG_S_O). Among the types of buffer zones identified in this study, Type II-A, Type II-B and Type III were found to have blue-green connectivity. However, initially, blue-green connectivity in these buffer zone types were not considered leading to poor design and poor performance. As such, improvement in the design considering blue-green network and renovation must be considered to optimize the performance of these buffer zones. The findings in this study is useful for designing buffer zones in the future.

• Korean Journal of Plant Resources
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• v.36 no.5
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• pp.517-526
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• 2023

The harvest time of the late-ripening 'Fuji' apple (Malus × domestica) is variable, depending on the coloration of the fruit skin. Ethephon, a plant growth regulator, promotes the ethylene production and induces physiological responses associated with fruit maturation in climacteric fruit crops, such as apples. This study aimed to investigate the effect of ethephon treatment on fruit characteristics after fruit enlargement, with the objective of proposing an economical and stable harvest control method for 'Fuji'/M.26 apples. Fruit characteristics were assessed at 10-days intervals following the application of 100 mg/L ethephon and mixture of 100 mg/L ethephon and 0.5% CaCl₂ at 145 days after full bloom (DAFB). Starch contents of ethephon-treated (ET) and ethephon with CaCl₂-treated (EC) apples began to decrease from 155 DAFB, and the starch contents of ET and EC at 10 days before harvest were similar to those of control at harvest time. Red coloration of fruit skin in EC was lower compared to ET but higher than control. The average fruit firmness was low in ET, while the control and EC exhibited similar levels of firmness. Fruit sugar acid ratios did not show significant differences between treatments. However, the titratable acidity of EC was significantly lower than that of the control at 10 days before harvest. Moreover, fruit sugar acid ratio of ET and EC at 10 days before harvest in 2021 was similar to their sugar acid ratio at harvest time. Therefore, it was thought that fruit maturation and skin coloration could be accelerated by 10 days from the harvest time through the combined treatment of 100 mg/L ethephon and 0.5% CaCl₂ at the end of fruit enlargement in 'Fuji'/M.26.

• Journal of Practical Agriculture & Fisheries Research
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• v.25 no.4
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• pp.24-38
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• 2024

Five species of plants (Pimpinella brachycarpa (Kom.) Nakai, Hylotelephium erythrostictum (Miq.) H. Ohba, Magnolia sieboldii K. Koch, Sanguisorba hakusanensis Makino, Agastache rugosa (Fisch. & Mey.) Kuntze) native to the clean area of Mt. Jiri were selected. The collection period was from May to September 2021, and the five species plants were collected in their native habitats with flowers in full bloom. The collected plants were extracted with 70% EtOH and 17 kinds of polyphenol components were analyzed. Next, flowers, leaves and roots were separated from plants, extracted with 70% EtOH for each part and experiments were conducted on DPPH, ABTS, total polyphenols, and total flavonoids. The results are as follows. 1. There were a total of 13 polyphenol components contained in the 5 species of plants native to Mt. Jiri. Among them, the total content of the most detected substance was Sanguisorba hakusanensis Makino, which was 126.2 ppm, and the main substance was ellagic acid (=ELA), which was 122.4 ppm. 2. As a result of examining the DPPH radical scavenging activity, the flowers, stems, and roots of Sanguisorba hakusanensis Makino showed the best scavenging activity, while the flower extracts of other plants showed good scavenging activity. 3. The highest ABTS radical scavenging activity was Sanguisorba hakusanensis Makino flower extract at 13.5 ㎍/㎖, followed by roots at 16.8 ㎍/㎖ and leaves at 22.6 ㎍/㎖;. Although such a large ABTS radical scavenging activity could not be confirmed in other plants, the flower extract was overall better than the leaf and root extracts of other parts. 4. The highest total polyphenol content was found in the leaves of Magnolia sieboldii K. Koch, at 161.4mg GAE/g, followed by the highest content in the roots of Magnolia sieboldii K. Koch and the roots of Agastache rugosa (Fisch. & Mey.) Kuntze, at 130.0mg GAE/g. Also, the lowest was found to be Sanguisorba hakusanensis Makino. 5. The total flavonoid content was 186.2mg CAE/g in the roots of Agastache rugosa (Fisch. & Mey.) Kuntze, 166.9mg CAE/g in the flowers, and the lowest was 116.1mg CAE/g in the leaves. As shown in the results above, Sanguisorba hakusanensis Makino has excellent antioxidant efficacy and has a high polyphenol content, so it is expected to be fully utilized in the cosmetics industry in the future.